FastQCFastQC Report
Wed 25 May 2016
SRR1294661_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294661_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876834
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC138810.3580498932892149No Hit
CCCATGTACTCTGCGTTGATACCAC127430.32869604424641347No Hit
GTATCAACGCAGAGTACTTTTTTTT72410.1867761168004614No Hit
GAGTACATGGGAAGCAGTGGTATCA64240.16570221990417955No Hit
CATGTACTCTGCGTTGATACCACTG51060.13170540704089986No Hit
GGTATCAACGCAGAGTACTTTTTTT48850.12600487923909046No Hit
TATCAACGCAGAGTACTTTTTTTTT42260.10900647280745063No Hit
GCGTTGATACCACTGCTTCCCATGT40120.1034865047097709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT500.001503343213.2963684
GTACGAC655.4712473E-513.1500853
GTCGTAC701.0925214E-412.2133161
TACGACC951.044531E-611.9967234
CCGTACC700.001495676110.8541784
AAATCGC1158.776533E-710.74396317
CGCCTTT2150.010.60914914
CTAGGAC3700.010.5240163
ATCGCCG1106.0648035E-610.36174219
TCGCGCG1856.002665E-1110.2674669
GCGCCTT2053.6379788E-1210.19957813
CGCGCGC1901.0004442E-109.9972710
ATCGCGC2054.1836756E-119.7289248
GCGCGCC2054.1836756E-119.72842211
GCGACTA2650.09.68335116
CTTAGAC3450.09.6349253
GTCCTAG3850.09.6226121
AATCGCC1304.213025E-69.50525718
CGCGCCT2002.6375346E-109.50402912
GTCTAGG3500.09.4992451
Produced by FastQC (version 0.11.5)