##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294661_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3876834 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.16655368788037 31.0 30.0 34.0 26.0 34.0 2 30.364339561611356 31.0 30.0 34.0 26.0 34.0 3 30.266589954586657 31.0 30.0 34.0 25.0 34.0 4 33.81549145514097 35.0 35.0 37.0 28.0 37.0 5 34.21112072376584 37.0 35.0 37.0 30.0 37.0 6 34.327819039969214 37.0 35.0 37.0 30.0 37.0 7 34.210409576474 37.0 35.0 37.0 30.0 37.0 8 34.03653806172769 37.0 35.0 37.0 28.0 37.0 9 35.19291359908627 39.0 35.0 39.0 27.0 39.0 10 35.293490513135204 39.0 35.0 39.0 28.0 39.0 11 35.555413515254976 39.0 35.0 39.0 29.0 39.0 12 35.32402109556406 39.0 35.0 39.0 27.0 39.0 13 35.208776800863795 39.0 35.0 39.0 27.0 39.0 14 36.11479289543994 39.0 36.0 41.0 27.0 41.0 15 36.11448336451858 39.0 36.0 41.0 27.0 41.0 16 36.21858789930134 39.0 36.0 41.0 27.0 41.0 17 36.18505718841715 39.0 36.0 41.0 27.0 41.0 18 36.063093235356476 39.0 35.0 41.0 27.0 41.0 19 36.337110642343724 39.0 36.0 41.0 27.0 41.0 20 36.45576983693395 39.0 36.0 41.0 27.0 41.0 21 36.4869483707582 39.0 36.0 41.0 27.0 41.0 22 36.47651073014733 39.0 36.0 41.0 27.0 41.0 23 36.44082284668366 39.0 36.0 41.0 27.0 41.0 24 36.453339503316364 39.0 36.0 41.0 27.0 41.0 25 35.233371869933045 39.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9921645470084322 1101 2 0.9499354394916608 1101 3 1.2519352087684048 1101 4 1.022487210067652 1101 5 0.7308966095932874 1101 6 0.7404503238587452 1101 7 0.7136414935885398 1101 8 0.8733190754502829 1101 9 1.1273423138870484 1101 10 1.1188663000471237 1101 11 0.8168747596434329 1101 12 1.0784151213258255 1101 13 1.210698218194139 1101 14 1.276903175239454 1101 15 1.3718056766034579 1101 16 1.305848808387296 1101 17 1.3193580560703495 1101 18 1.3518988677486945 1101 19 1.2372332864414943 1101 20 1.1354976725245436 1101 21 1.0457778041978543 1101 22 0.9315247688103767 1101 23 0.9816640721499184 1101 24 0.9959154604307372 1101 25 0.9654063970346769 1102 1 0.809145462724711 1102 2 0.8309177886499874 1102 3 1.1030738313809714 1102 4 0.9544861103871582 1102 5 0.7282366954482953 1102 6 0.6509545453345211 1102 7 0.588370448246252 1102 8 0.6897872454378771 1102 9 0.9587570084969244 1102 10 0.8303010431665001 1102 11 0.6900942293786088 1102 12 0.8928576381980804 1102 13 0.8323716744413545 1102 14 1.093648529168675 1102 15 1.186412863909979 1102 16 1.0954765532057351 1102 17 1.1560966787969988 1102 18 1.1692617625444157 1102 19 0.9078863394728813 1102 20 0.10465996971168323 1102 21 0.38873441139938336 1102 22 0.4685112581520485 1102 23 0.6308422146328354 1102 24 0.7137585715033694 1102 25 0.7364590744434949 1103 1 0.605595852395659 1103 2 0.6912877988319508 1103 3 0.9345047476771917 1103 4 0.7614602141575375 1103 5 0.5806484534731666 1103 6 0.5189945815963952 1103 7 0.4108736733443621 1103 8 0.6416143346343333 1103 9 0.6650213336899 1103 10 0.6399560063909959 1103 11 0.4692382225579834 1103 12 0.815406490465783 1103 13 0.6755650166367033 1103 14 0.6424301234761387 1103 15 0.8917318254166133 1103 16 0.7595116533238766 1103 17 0.8252217978977257 1103 18 0.8730863744600441 1103 19 0.7915821559150942 1103 20 -0.13994741854173043 1103 21 0.26717973029021636 1103 22 -0.2827952358545218 1103 23 0.28529776860121814 1103 24 0.36814573647312443 1103 25 0.515574941040974 1104 1 0.3368271054757841 1104 2 0.3437825055944792 1104 3 0.6333184654490367 1104 4 0.4273818901495616 1104 5 0.3185573923127123 1104 6 0.22285821162137154 1104 7 0.25483269541557263 1104 8 0.31264184577379694 1104 9 0.6783495283940155 1104 10 0.5826514813372157 1104 11 0.3624687740340491 1104 12 0.507614904821331 1104 13 0.44344103015891534 1104 14 0.7270567985683343 1104 15 0.728505468870928 1104 16 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0.38328434649196197 2207 7 0.2923095622558236 2207 8 0.40228125166679973 2207 9 -1.1731200491091514 2207 10 -0.09163133330819306 2207 11 0.20310761916956466 2207 12 0.5549356933106466 2207 13 0.6298593715719534 2207 14 0.82849104171666 2207 15 0.7624344093148068 2207 16 0.7412141202478821 2207 17 0.7122483688234169 2207 18 0.8302358914113839 2207 19 -0.305206150223448 2207 20 0.22730199846851207 2207 21 -0.27372898127675427 2207 22 0.18521078523777135 2207 23 -0.5907123308542026 2207 24 -1.6784983699090787 2207 25 -0.36231485149060205 2208 1 0.5621744179362906 2208 2 0.2451191726971622 2208 3 0.6337905600142157 2208 4 0.5432700024691357 2208 5 0.4750494088524775 2208 6 0.37603154526861005 2208 7 0.4291866406076821 2208 8 0.5507989494684793 2208 9 -0.13977601077356638 2208 10 0.08390345454299108 2208 11 0.28553976972990824 2208 12 0.2921838784072719 2208 13 0.682402215099529 2208 14 0.8418590916966906 2208 15 0.8267210620260457 2208 16 0.6103419642507006 2208 17 0.5020860453059228 2208 18 0.8425765531297458 2208 19 -1.273453232056056 2208 20 0.01579999257815956 2208 21 -0.1849046792347906 2208 22 0.21796809213169155 2208 23 0.42680040404355424 2208 24 0.5932846714858613 2208 25 0.5996245186205797 2209 1 0.2300038117092562 2209 2 -1.3845720855307668 2209 3 -0.11639951676106364 2209 4 0.19965454718177966 2209 5 0.19994268057615727 2209 6 0.1690642738613306 2209 7 0.2093194344845557 2209 8 0.3624072288749005 2209 9 -1.9743046182528445 2209 10 -0.2751803221236031 2209 11 0.008829596040158094 2209 12 0.37491870301739993 2209 13 0.4406201090850246 2209 14 0.6347797439908476 2209 15 0.798673377707452 2209 16 0.6700935227531772 2209 17 0.5468207483737828 2209 18 0.7842187389157189 2209 19 0.4942784065502579 2209 20 0.4348150832061606 2209 21 0.4457121249109406 2209 22 0.49070502336565625 2209 23 0.40899513135796184 2209 24 0.5125515794231461 2209 25 0.558785880304427 2210 1 0.035776314516930086 2210 2 0.14211579863182777 2210 3 0.033690731907579874 2210 4 0.13785231163889478 2210 5 0.01944984314994258 2210 6 0.004779044058736304 2210 7 -0.014306242009588743 2210 8 0.11122401571241625 2210 9 0.20132981534146666 2210 10 -1.5358850191339215 2210 11 -0.49995828616919624 2210 12 -2.4479824787081697 2210 13 -0.8308357052475728 2210 14 -0.5010331578806912 2210 15 -0.7761434814905286 2210 16 -0.25337517767670903 2210 17 -0.050663068800815836 2210 18 0.21946122107099342 2210 19 0.05082314308027236 2210 20 0.10502278659615172 2210 21 0.15863841604875262 2210 22 0.03128334833948543 2210 23 -0.055755834485346156 2210 24 0.026015184126642055 2210 25 -0.026806804266477968 2211 1 -0.4177061452592312 2211 2 -0.1624628753620243 2211 3 -1.545923147950898 2211 4 -0.6874677868840919 2211 5 -0.5546804748627281 2211 6 -0.4682388827409838 2211 7 -0.4939966406931475 2211 8 -0.1376151448268388 2211 9 0.00902998579201153 2211 10 -0.036763006806033616 2211 11 -0.3173774264452405 2211 12 -0.45615862686373276 2211 13 -0.3676880208469768 2211 14 -0.8682482611716225 2211 15 -0.4904517985069603 2211 16 -0.18716098653733582 2211 17 -0.37990587751881577 2211 18 -0.1946197665003524 2211 19 -0.1618189865176447 2211 20 -0.2223348204234199 2211 21 -0.3704658169253534 2211 22 -0.4303161708977967 2211 23 -0.6979659812552512 2211 24 -0.44347932932949874 2211 25 -0.411137892493727 2212 1 -0.43538314582538007 2212 2 -0.4305873834732168 2212 3 -2.439296098747782 2212 4 -1.444084966120343 2212 5 -0.6465833399075294 2212 6 -0.48261372717563944 2212 7 -0.3285399795889177 2212 8 -0.4105691066658821 2212 9 -0.9470238794075669 2212 10 -0.35492231266202623 2212 11 -0.3995376880588921 2212 12 -0.11921327878681609 2212 13 -0.5517154659609815 2212 14 -0.2047816235071238 2212 15 -0.052054201158092894 2212 16 -1.1746725392878332 2212 17 -1.296403027955435 2212 18 -0.32909910083500193 2212 19 -0.2238773782792265 2212 20 -0.23114109262407823 2212 21 -0.11909259643521608 2212 22 -0.3976360928950129 2212 23 -0.09008912813584402 2212 24 -0.14261528828178172 2212 25 -0.09759569331880158 2213 1 -3.1152636795609183 2213 2 -1.3435347640558106 2213 3 -2.3889878052001308 2213 4 -1.0014219042110213 2213 5 -0.757449746228481 2213 6 -0.6265618389028091 2213 7 -0.3932574830290889 2213 8 -0.22228098512114514 2213 9 -0.25608234108398165 2213 10 -0.0769055940526755 2213 11 -0.38766923430321043 2213 12 -0.2449166893003536 2213 13 -0.5079443636455778 2213 14 -0.18257095155536973 2213 15 -0.2985546799658181 2213 16 -1.6892836473972181 2213 17 -1.0581165733283626 2213 18 -0.2601954785654357 2213 19 -0.11357959206084445 2213 20 -0.11825383603716944 2213 21 0.030726945357855584 2213 22 0.020567826315812 2213 23 0.014050403218874408 2213 24 0.058666456440697345 2213 25 0.0330264175967514 2214 1 -0.8383836911810754 2214 2 -0.6635978741605051 2214 3 -6.2288992201402245 2214 4 -2.087824926196202 2214 5 -1.2209049173836775 2214 6 -0.7804246093414307 2214 7 -0.48693287846818123 2214 8 -0.3199163580648019 2214 9 -0.24311907120215892 2214 10 -0.052986976106240036 2214 11 -0.8253244955891006 2214 12 -0.3350678630477404 2214 13 -5.095622064493625 2214 14 -3.5629322378171437 2214 15 -2.1405471082430196 2214 16 -2.7403238747351253 2214 17 -3.351871857333144 2214 18 -1.083084483738645 2214 19 -0.6327710043193591 2214 20 -0.4030589004880625 2214 21 -0.40768297423215927 2214 22 -0.37213521913314906 2214 23 -0.36481599851818913 2214 24 -0.10198311767317136 2214 25 0.02831178178804805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1502.0 3 207.0 4 161.0 5 462.0 6 76.0 7 78.0 8 240.0 9 663.0 10 1412.0 11 2999.0 12 5200.0 13 8532.0 14 12822.0 15 17471.0 16 21088.0 17 23488.0 18 24659.0 19 25639.0 20 26313.0 21 26825.0 22 27866.0 23 28929.0 24 31297.0 25 34250.0 26 37603.0 27 42904.0 28 50052.0 29 61106.0 30 75181.0 31 94845.0 32 123118.0 33 164260.0 34 220695.0 35 303778.0 36 427852.0 37 648230.0 38 1305031.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65111980907513 21.4052539455199 12.762175062491336 23.18145118291364 2 14.540029710135203 21.786262520736706 41.319927985440174 22.35377978368792 3 17.902252105420864 27.968294352947797 30.087510042865905 24.041943498765434 4 11.669408733062095 17.700690226501138 39.72202543863384 30.907875601802925 5 12.082134504063395 39.27969855696612 35.97342011958314 12.664746819387343 6 29.504185284637813 39.82993514222017 18.15222825849879 12.513651314643218 7 24.983655183610427 33.903782266655 24.187927833473616 16.924634716260954 8 23.804950493516227 37.921925273368394 21.845700619598897 16.427423613516474 9 24.589685361344806 16.92708884519266 22.48975407191431 35.993471721548225 10 14.09223157629558 30.896163590973007 34.60478007603024 20.406824756701173 11 31.721678146964326 26.547336612238492 23.619912328518218 18.111072912278964 12 20.013803388437623 28.39647110231723 32.85534239914145 18.734383110103703 13 26.116228883542696 24.552738006100032 27.46959728288742 21.861435827469855 14 19.58853544646823 23.886749294777104 29.264536857817415 27.260178400937253 15 22.51395187303944 31.240709398636263 24.753917636390995 21.4914210919333 16 20.63711493099711 31.96118214857876 26.93839541214479 20.463307508279343 17 19.454134399439173 31.070078184708567 29.292873759739535 20.182913656112724 18 19.851296160300922 30.401653803264466 30.163388757165194 19.583661279269418 19 20.31383578446556 29.789155133748807 30.315596180611482 19.581412901174147 20 21.582637253834093 29.086254664691953 29.437439053789593 19.893669027684357 21 21.5806210912083 29.659339068185098 28.958773128187076 19.801266712419526 22 20.48640651198903 29.402732119700335 29.686532060230114 20.424329308080516 23 20.32645920382754 29.67568315404637 29.532506538063245 20.46535110406284 24 20.405428533592232 29.31661635896492 29.523001414363627 20.75495369307922 25 20.90213612602204 29.005997755358898 28.812844013963446 21.279022104655617 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4009.0 1 4009.0 2 3969.5 3 3930.0 4 3930.0 5 3930.0 6 6384.0 7 8838.0 8 8838.0 9 8838.0 10 14178.0 11 19518.0 12 19518.0 13 19518.0 14 32642.0 15 45766.0 16 45766.0 17 45766.0 18 70108.5 19 94451.0 20 94451.0 21 94451.0 22 131381.0 23 168311.0 24 168311.0 25 168311.0 26 216112.5 27 263914.0 28 263914.0 29 263914.0 30 311310.5 31 358707.0 32 358707.0 33 358707.0 34 395849.5 35 432992.0 36 432992.0 37 432992.0 38 449497.0 39 466002.0 40 466002.0 41 466002.0 42 459801.0 43 453600.0 44 453600.0 45 453600.0 46 459284.0 47 464968.0 48 464968.0 49 464968.0 50 424681.5 51 384395.0 52 384395.0 53 384395.0 54 332933.0 55 281471.0 56 281471.0 57 281471.0 58 238092.0 59 194713.0 60 194713.0 61 194713.0 62 159668.0 63 124623.0 64 124623.0 65 124623.0 66 93710.5 67 62798.0 68 62798.0 69 62798.0 70 45002.5 71 27207.0 72 27207.0 73 27207.0 74 19067.0 75 10927.0 76 10927.0 77 10927.0 78 7369.5 79 3812.0 80 3812.0 81 3812.0 82 2578.5 83 1345.0 84 1345.0 85 1345.0 86 860.0 87 375.0 88 375.0 89 375.0 90 242.0 91 109.0 92 109.0 93 109.0 94 71.0 95 33.0 96 33.0 97 33.0 98 26.5 99 20.0 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10052016671335424 2 0.05318772998792313 3 0.056566775879493415 4 0.05207857751969777 5 0.04831261797642097 6 0.0687416587865253 7 0.06492411075635429 8 0.06169983032546661 9 0.06737456388382891 10 0.06670391355420428 11 0.06113235696963037 12 0.06249945187232675 13 0.06260262884611516 14 0.0651820531908253 15 0.06765830056174704 16 0.06270580581990357 17 0.06569793805976733 18 0.14225525261076435 19 0.06979922276785645 20 0.06489831651290717 21 0.061777213055807904 22 0.06773568329208833 23 0.0670134444755695 24 0.07756329004543398 25 0.07010875368922166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3876834.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.5070374642439 #Duplication Level Percentage of deduplicated Percentage of total 1 87.2929819450864 66.78527440038292 2 9.38551755565457 14.361162865035663 3 1.905845323738555 4.374317387529591 4 0.5562442941860632 1.7022641221826018 5 0.2505044670076184 0.9582677321156154 6 0.13141862315471156 0.6032669715118122 7 0.0910238973470994 0.4874778107133217 8 0.058509464634081736 0.3581108642218035 9 0.04262895394220446 0.29352734786859874 >10 0.2506859277052588 3.4972175637153713 >50 0.01961220116969184 1.0416122709879685 >100 0.012210212401010044 1.7437212394849255 >500 0.0014255377432451405 0.73350509771848 >1k 0.0012218893722813612 1.8814779493324565 >5k 1.0182411435677727E-4 0.48743665153431837 >10k+ 6.788274290451819E-5 0.6913597256645726 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 13881 0.3580498932892149 No Hit CCCATGTACTCTGCGTTGATACCAC 12743 0.32869604424641347 No Hit GTATCAACGCAGAGTACTTTTTTTT 7241 0.1867761168004614 No Hit GAGTACATGGGAAGCAGTGGTATCA 6424 0.16570221990417955 No Hit CATGTACTCTGCGTTGATACCACTG 5106 0.13170540704089986 No Hit GGTATCAACGCAGAGTACTTTTTTT 4885 0.12600487923909046 No Hit TATCAACGCAGAGTACTTTTTTTTT 4226 0.10900647280745063 No Hit GCGTTGATACCACTGCTTCCCATGT 4012 0.1034865047097709 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.158848689420285E-5 0.0 5 0.0 0.0 0.0 5.158848689420285E-5 0.0 6 0.0 0.0 0.0 5.158848689420285E-5 0.0 7 0.0 0.0 0.0 5.158848689420285E-5 0.0 8 2.5794243447101424E-5 0.0 0.0 5.158848689420285E-5 0.0 9 2.5794243447101424E-5 0.0 0.0 1.031769737884057E-4 0.0 10 2.5794243447101424E-5 0.0 0.0 1.031769737884057E-4 0.0 11 2.5794243447101424E-5 5.158848689420285E-5 0.0 1.5476546068260853E-4 0.0 12 2.5794243447101424E-5 5.158848689420285E-5 0.0 1.5476546068260853E-4 0.0 13 2.5794243447101424E-5 5.158848689420285E-5 0.0 1.8055970412970997E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGCT 50 0.0015033432 13.296368 4 GTACGAC 65 5.4712473E-5 13.150085 3 GTCGTAC 70 1.0925214E-4 12.213316 1 TACGACC 95 1.044531E-6 11.996723 4 CCGTACC 70 0.0014956761 10.854178 4 AAATCGC 115 8.776533E-7 10.743963 17 CGCCTTT 215 0.0 10.609149 14 CTAGGAC 370 0.0 10.524016 3 ATCGCCG 110 6.0648035E-6 10.361742 19 TCGCGCG 185 6.002665E-11 10.267466 9 GCGCCTT 205 3.6379788E-12 10.199578 13 CGCGCGC 190 1.0004442E-10 9.99727 10 ATCGCGC 205 4.1836756E-11 9.728924 8 GCGCGCC 205 4.1836756E-11 9.728422 11 GCGACTA 265 0.0 9.683351 16 CTTAGAC 345 0.0 9.634925 3 GTCCTAG 385 0.0 9.622612 1 AATCGCC 130 4.213025E-6 9.505257 18 CGCGCCT 200 2.6375346E-10 9.504029 12 GTCTAGG 350 0.0 9.499245 1 >>END_MODULE