FastQCFastQC Report
Wed 25 May 2016
SRR1294661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876834
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT177670.458286323324651No Hit
CCCATGTACTCTGCGTTGATACCAC142310.36707787849570034No Hit
GTACATGGGAAGCAGTGGTATCAAC137050.353510106442525No Hit
GGTATCAACGCAGAGTACTTTTTTT133220.34363091120228517No Hit
TATCAACGCAGAGTACTTTTTTTTT115190.2971238902671613No Hit
ACGCAGAGTACTTTTTTTTTTTTTT72310.18651817436599039No Hit
GAGTACATGGGAAGCAGTGGTATCA68920.17777392583742302No Hit
CATGTACTCTGCGTTGATACCACTG57300.14780101495189116No Hit
GCGTTGATACCACTGCTTCCCATGT45170.11651259765055712No Hit
ACGCAGAGTACATGGGAAGCAGTGG41000.10575639813311584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC250.00600212719.0172481
TACGACC907.309789E-611.6335364
TTACACG1201.2435339E-711.1047394
TAGGCGT700.001468444210.8781126
CGCAATT700.001512784510.83938812
AGCACCG1158.6108776E-710.75987155
CCCCGTA1909.094947E-1210.4828315
CGACCAT1002.350326E-510.4701826
ACCGTTC750.0026070310.15290458
ATAGGCG856.4816716E-410.078255
GTACGAC951.646717E-49.9991233
GGGTACG1106.7741836E-59.5086251
GGTACGA1106.848334E-59.49916652
ATCGCCG1002.802548E-49.48458719
CCCGTAC2106.91216E-119.48434316
CGCCCTA2750.09.311916
CGCGCGC2151.0186341E-109.2967510
TCGCGCG2054.0927262E-109.2859539
TATCGCG1951.6279955E-99.2742887
AACCGTG850.00732212658.9584457
Produced by FastQC (version 0.11.5)