FastQCFastQC Report
Wed 25 May 2016
SRR1294660_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294660_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228804
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC62590.5093570658949678No Hit
CCCATGTACTCTGCGTTGATACCAC59330.4828272043385275No Hit
GTATCAACGCAGAGTACTTTTTTTT33040.26887933307508766No Hit
GAGTACATGGGAAGCAGTGGTATCA29230.2378735746302909No Hit
GGTATCAACGCAGAGTACTTTTTTT25550.20792575545001482No Hit
CATGTACTCTGCGTTGATACCACTG24180.19677670320083596No Hit
TATCAACGCAGAGTACTTTTTTTTT21380.17399031904193019No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18640.15169221454357243No Hit
GCGTTGATACCACTGCTTCCCATGT17740.14436801963535276No Hit
ACGCAGAGTACATGGGAAGCAGTGG15370.12508097304370755No Hit
GTATCAACGCAGAGTACATGGGAAG14250.11596641938014525No Hit
TATCAACGCAGAGTACATGGGAAGC12890.10489874707439104No Hit
ACTCTGCGTTGATACCACTGCTTCC12290.10001595046891124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT350.002173705716.2813878
CGATTTC551.953895E-413.82295212
GGCGATT551.9640467E-413.81451110
TTCCGAA655.4309712E-513.15892316
ATTTCCG655.4330478E-513.15838614
TTTCCGA604.0790447E-412.67103915
GTATTGG751.4783103E-512.6663951
GCGATTT604.1001203E-412.66330111
CCGAATG853.9226543E-612.29936318
CTAGGAC1353.7289283E-1011.9607583
AGCGTAC1603.6379788E-1211.8747461
TCCGAAT752.0657218E-411.404417
CGCCCTA1701.0913936E-1111.18078316
TAGGACT2000.010.9225424
GTCCCGC1752.0008883E-1110.8608912
CGGCGAT700.00149432510.8542599
GCCTTAA803.7702048E-410.6872711
CTACACT1355.6988938E-810.5531814
TAGACAG1106.0569528E-610.3613045
GCTACAC1303.8467624E-710.2288843
Produced by FastQC (version 0.11.5)