##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294660_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1228804 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.94766130318586 31.0 30.0 33.0 25.0 34.0 2 30.132424699138348 31.0 30.0 34.0 25.0 34.0 3 30.074788168007267 31.0 30.0 34.0 25.0 34.0 4 33.68467957461076 35.0 35.0 37.0 28.0 37.0 5 34.05318993102236 35.0 35.0 37.0 28.0 37.0 6 34.15164257277808 37.0 35.0 37.0 30.0 37.0 7 34.0917151962396 37.0 35.0 37.0 29.0 37.0 8 33.9624569906999 37.0 35.0 37.0 28.0 37.0 9 35.037971067802516 39.0 35.0 39.0 27.0 39.0 10 35.19107441056507 39.0 35.0 39.0 27.0 39.0 11 35.401562006634094 39.0 35.0 39.0 28.0 39.0 12 35.20186701866205 39.0 35.0 39.0 27.0 39.0 13 35.04257228980374 38.0 35.0 39.0 27.0 39.0 14 35.943401876947014 39.0 35.0 41.0 27.0 41.0 15 35.9427337476115 39.0 35.0 41.0 27.0 41.0 16 36.05214012975218 39.0 35.0 41.0 27.0 41.0 17 36.050331867409284 39.0 35.0 41.0 27.0 41.0 18 35.92271672292733 39.0 35.0 41.0 27.0 41.0 19 36.20233983613335 39.0 35.0 41.0 27.0 41.0 20 36.315020133398 39.0 36.0 41.0 27.0 41.0 21 36.35088264686638 39.0 36.0 41.0 27.0 41.0 22 36.37357218889262 39.0 36.0 41.0 27.0 41.0 23 36.355205549461104 39.0 36.0 41.0 27.0 41.0 24 36.36907187802123 39.0 35.0 41.0 27.0 41.0 25 35.17041285672898 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9563794437146171 1101 2 1.0049932359669533 1101 3 1.2936911657646952 1101 4 1.0217145456709744 1101 5 0.7576379772745767 1101 6 0.6425948600942206 1101 7 0.5810256619781384 1101 8 0.6975231177070214 1101 9 1.1123526210407704 1101 10 1.0706217381587138 1101 11 0.9031603770319094 1101 12 1.0515707462549102 1101 13 1.1767029758420335 1101 14 1.1448632947330708 1101 15 1.24459791853959 1101 16 1.2535977351899845 1101 17 1.2602411507762312 1101 18 1.2884112038694084 1101 19 1.0587711912754116 1101 20 1.0176557658154834 1101 21 0.8509768461147118 1101 22 0.888269257962655 1101 23 0.9787455754285759 1101 24 0.9423746593557709 1101 25 1.1590784288724265 1102 1 0.5802236788323683 1102 2 0.6967606358747815 1102 3 1.0523043734302853 1102 4 0.9279378605522339 1102 5 0.6624057483756616 1102 6 0.5726089726106096 1102 7 0.4473822836533117 1102 8 0.6720752201491891 1102 9 0.9140520405806001 1102 10 0.7362598229449944 1102 11 0.6776506139321086 1102 12 0.7365919329615309 1102 13 0.750614187133479 1102 14 0.8867179340210924 1102 15 0.94809610112722 1102 16 0.7501967442653878 1102 17 0.8216653852125688 1102 18 0.867573454770941 1102 19 0.7826084328971135 1102 20 0.06797504580816849 1102 21 0.27430517640856067 1102 22 0.3787416058585933 1102 23 0.583263194555613 1102 24 0.666675264920805 1102 25 0.6268866492787311 1103 1 0.7007726354474357 1103 2 0.7982207264911594 1103 3 1.125200856102964 1103 4 0.8692079304953992 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0.45656667174984733 2207 8 0.3383817717409343 2207 9 -1.2600667050423127 2207 10 -0.1361089412765466 2207 11 0.14879451943910027 2207 12 0.4510426833627932 2207 13 0.6251701501961762 2207 14 0.8723539772246411 2207 15 0.8354349236928726 2207 16 0.9685176343316684 2207 17 0.9100318782635171 2207 18 0.8716184643096838 2207 19 -0.27456384000934264 2207 20 0.39973303958468165 2207 21 -0.12041691730971849 2207 22 0.30774221384138656 2207 23 -0.6086717555615806 2207 24 -1.6060008256215994 2207 25 -0.08193388976513916 2208 1 0.4300892128781193 2208 2 0.11618273583536265 2208 3 0.6646477066980587 2208 4 0.5331952442081871 2208 5 0.2524507356815633 2208 6 0.15914903747920306 2208 7 0.27749222506976423 2208 8 0.4800316711373114 2208 9 -0.3119781738551879 2208 10 -0.16065622497875864 2208 11 0.0868226884531822 2208 12 0.11301451434870557 2208 13 0.21470737353621416 2208 14 0.6373439168624699 2208 15 0.6084731530691343 2208 16 0.3673103908709052 2208 17 0.38769787423937174 2208 18 0.5452603154163285 2208 19 -1.3542419084198016 2208 20 -0.08920056202497761 2208 21 -0.2214229535270249 2208 22 0.07152491001844652 2208 23 0.2158755281406286 2208 24 0.32075973775867794 2208 25 0.33416268971172514 2209 1 0.21109195559585103 2209 2 -1.373214194015496 2209 3 -0.36412932577888313 2209 4 -0.018190303883699244 2209 5 0.027122444919911004 2209 6 0.2502390819174707 2209 7 0.17215987339115912 2209 8 0.4418545751885574 2209 9 -2.2424725425304857 2209 10 -0.39370841175855276 2209 11 -0.026055134703476313 2209 12 0.23802840380383117 2209 13 0.4388328974384734 2209 14 0.6166938344050692 2209 15 0.6489847966293425 2209 16 0.5313530496843342 2209 17 0.38147347979963087 2209 18 0.6611002097685343 2209 19 0.24541291363928508 2209 20 0.3054169643489999 2209 21 0.3866411641948062 2209 22 0.3303461729156538 2209 23 0.4730033566931908 2209 24 0.5527637603047637 2209 25 0.6335542716227351 2210 1 0.11786370673119251 2210 2 0.13576161260474962 2210 3 -0.03709336172202882 2210 4 0.1202135261486319 2210 5 -0.027397251764647024 2210 6 6.349545766894948E-4 2210 7 -0.12413251539856418 2210 8 0.07465250619550545 2210 9 0.32353506506798624 2210 10 -1.6987047714409442 2210 11 -0.5711294903292412 2210 12 -2.5637301642491934 2210 13 -0.8505980154163169 2210 14 -0.827075614680858 2210 15 -0.999390937374649 2210 16 -0.29205970530423286 2210 17 -0.05424212300084719 2210 18 0.3392581140101214 2210 19 0.14768464291371686 2210 20 0.08430426277710268 2210 21 0.17470244319140704 2210 22 0.07721198301463517 2210 23 -0.013709959265462146 2210 24 0.009019118605159804 2210 25 0.09301035304419258 2211 1 -0.29817271864309447 2211 2 0.03063993744911997 2211 3 -1.2872477065283263 2211 4 -0.5387616243375533 2211 5 -0.533262354403945 2211 6 -0.32168844924077433 2211 7 -0.35586837998671683 2211 8 -0.021774411538714844 2211 9 0.1680198106562969 2211 10 0.09672404987922079 2211 11 -0.23325584727733428 2211 12 -0.40965546136294506 2211 13 -0.2854516450916833 2211 14 -0.7937371365206474 2211 15 -0.26986734834341775 2211 16 -0.01435481257387039 2211 17 -0.19038710422222493 2211 18 0.05805473900369407 2211 19 0.10607585554940613 2211 20 0.007234756166006662 2211 21 -0.34828207087969787 2211 22 -0.14553329660670045 2211 23 -0.6725822132141204 2211 24 -0.35544041733145093 2211 25 -0.36183092904666836 2212 1 -0.327400704793849 2212 2 -0.24909627447101812 2212 3 -2.4880106012280976 2212 4 -1.1915277663442083 2212 5 -0.44376235069697145 2212 6 -0.4923433227959819 2212 7 -0.3099846009518643 2212 8 -0.35853422203833674 2212 9 -0.9825896154304061 2212 10 -0.28833759705400297 2212 11 -0.22583930973683408 2212 12 0.02905310396501193 2212 13 -0.26493467086995537 2212 14 0.04357167643298965 2212 15 0.21358065396973558 2212 16 -1.0428369560929767 2212 17 -1.1519988212172052 2212 18 -0.16572436998868056 2212 19 -0.11689995275568776 2212 20 0.05451939966656738 2212 21 -0.08066135335832314 2212 22 -0.19111644026666852 2212 23 0.16544982984606094 2212 24 -0.04371971537936048 2212 25 0.08487394413784699 2213 1 -3.0491748671774346 2213 2 -1.4834201914629404 2213 3 -2.331533807163872 2213 4 -0.9685826799740767 2213 5 -0.8102422363096764 2213 6 -0.7044235484790207 2213 7 -0.3865931722700253 2213 8 -0.28677358517935403 2213 9 -0.4411647980249853 2213 10 -0.15748587544794646 2213 11 -0.6281173945225476 2213 12 -0.24512280514346685 2213 13 -0.6484547852572504 2213 14 -0.2930535119582416 2213 15 -0.2540373079269287 2213 16 -1.7583043595786947 2213 17 -1.1100754916194973 2213 18 -0.30808631302689804 2213 19 -0.09427947897588496 2213 20 -0.11378256733114966 2213 21 -0.010525711668790905 2213 22 -0.009779731992914265 2213 23 -0.003938043555031356 2213 24 0.025631445883533388 2213 25 0.15032782209896567 2214 1 -0.6562972296385468 2214 2 -0.3343994457789137 2214 3 -6.420737830569259 2214 4 -2.227890028814283 2214 5 -1.1079242647765568 2214 6 -0.5895856291728023 2214 7 -0.3429949969875352 2214 8 -0.21022168873045644 2214 9 -0.08103697108300167 2214 10 0.10225347365017257 2214 11 -0.6411088351144514 2214 12 -0.2903143843623184 2214 13 -4.921902082837729 2214 14 -3.7450539310629694 2214 15 -1.9863398048239418 2214 16 -2.3206131430466286 2214 17 -3.3357238949916734 2214 18 -0.9637111820566702 2214 19 -0.551941035788758 2214 20 -0.11791639069462434 2214 21 -0.23980501273106114 2214 22 -0.1131994653778321 2214 23 0.01179530912159521 2214 24 0.04166220172279367 2214 25 0.2286963763488501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 473.0 3 61.0 4 57.0 5 127.0 6 29.0 7 17.0 8 31.0 9 98.0 10 301.0 11 737.0 12 1542.0 13 2713.0 14 4362.0 15 6372.0 16 7793.0 17 8767.0 18 9281.0 19 9434.0 20 9348.0 21 9245.0 22 9283.0 23 9524.0 24 9834.0 25 10832.0 26 11755.0 27 13631.0 28 15494.0 29 19029.0 30 23716.0 31 29757.0 32 39538.0 33 52103.0 34 71182.0 35 97836.0 36 138338.0 37 209330.0 38 396834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.464993597283815 20.657964620512804 12.259898143049387 23.617143639153994 2 15.358641612425721 21.745584051615342 39.29530469107495 23.60046964488398 3 18.802310177008806 26.828815492809806 29.576160849290506 24.792713480890882 4 12.099709323627836 17.997264222379638 37.880993673514254 32.02203278047827 5 12.114234722457434 39.575137110326594 35.45718640490861 12.85344176230736 6 30.16456374483499 38.81401397749464 17.71666175342475 13.304760524245626 7 25.84723851992518 34.25362311169452 22.502094837294862 17.397043531085433 8 24.404023229984006 37.82257985714146 20.853975512756623 16.91942140011791 9 24.859202413021457 17.583981811134166 21.220126318806777 36.33668945703759 10 14.599745601362873 31.245103803438777 33.80513259836774 20.3500179968306 11 31.872568354778767 26.344417862709047 22.820537457718483 18.962476324793702 12 20.491527618197644 28.548616545431006 31.26617911825994 19.693676718111412 13 27.047538393889546 23.99044020650457 26.265980489552625 22.696040910053256 14 20.02361505826405 24.638974617883928 27.958437497455275 27.378972826396748 15 23.245420809917906 31.007873772089763 24.09224422455682 21.65446119343551 16 20.7744185061773 31.2658178371459 26.62388318333871 21.33588047333809 17 20.397224800899007 30.545023696682467 27.99881109428185 21.058940408136674 18 20.758885639867128 29.525213604164836 29.148207550552712 20.567693205415328 19 20.861574593981405 30.02587215717763 29.34791903000177 19.7646342188392 20 21.828627267360194 28.959019563933957 28.80771971254657 20.404633456159278 21 21.5015955969829 29.127563886986625 28.77913012889362 20.591710387136857 22 21.269199103899307 28.93361248938303 28.130753827198497 21.666434579519166 23 21.014418402396064 29.126133741146276 28.58819199129652 21.271255865161145 24 21.23866346116258 29.512789440079224 28.275380164966187 20.973166933792005 25 21.381984251917206 28.645256071253193 28.116085309037192 21.85667436779241 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2816.0 1 2816.0 2 2706.5 3 2597.0 4 2597.0 5 2597.0 6 3368.0 7 4139.0 8 4139.0 9 4139.0 10 5817.5 11 7496.0 12 7496.0 13 7496.0 14 11198.0 15 14900.0 16 14900.0 17 14900.0 18 21668.0 19 28436.0 20 28436.0 21 28436.0 22 39155.5 23 49875.0 24 49875.0 25 49875.0 26 62993.0 27 76111.0 28 76111.0 29 76111.0 30 88802.0 31 101493.0 32 101493.0 33 101493.0 34 110713.0 35 119933.0 36 119933.0 37 119933.0 38 123043.5 39 126154.0 40 126154.0 41 126154.0 42 129394.5 43 132635.0 44 132635.0 45 132635.0 46 143268.0 47 153901.0 48 153901.0 49 153901.0 50 143706.5 51 133512.0 52 133512.0 53 133512.0 54 117764.0 55 102016.0 56 102016.0 57 102016.0 58 87806.0 59 73596.0 60 73596.0 61 73596.0 62 61710.0 63 49824.0 64 49824.0 65 49824.0 66 38686.5 67 27549.0 68 27549.0 69 27549.0 70 20447.5 71 13346.0 72 13346.0 73 13346.0 74 9436.5 75 5527.0 76 5527.0 77 5527.0 78 3792.0 79 2057.0 80 2057.0 81 2057.0 82 1355.5 83 654.0 84 654.0 85 654.0 86 409.5 87 165.0 88 165.0 89 165.0 90 107.0 91 49.0 92 49.0 93 49.0 94 29.0 95 9.0 96 9.0 97 9.0 98 11.5 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.097655932109596 2 0.05224592367863386 3 0.05476870192479842 4 0.05159488413123655 5 0.04850244628109934 6 0.06754535304247057 7 0.06388325558836072 8 0.059895638360552214 9 0.06510395473973067 10 0.0659177541739773 11 0.059081838926305585 12 0.061441857285620814 13 0.060546677907949514 14 0.06298807621068941 15 0.06534809457000465 16 0.06184875700274413 17 0.06380187564493604 18 0.14387973997480477 19 0.06860329230699119 20 0.06339497592781274 21 0.05997701830397688 22 0.06648741377794994 23 0.06429015530548403 24 0.07454402817699161 25 0.06876605219384051 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1228804.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.1141102353852 #Duplication Level Percentage of deduplicated Percentage of total 1 87.63953968015306 67.58245123873738 2 9.285570980841907 14.320970884302733 3 1.8351608351632125 4.245503848273126 4 0.565966524395591 1.7457601980711772 5 0.22421467647384946 0.8645057638997828 6 0.11520131645785725 0.5330188209955619 7 0.05827133431795008 0.3145479468710171 8 0.04722488712768648 0.2913364121454431 9 0.030697550232125552 0.21304928453007638 >10 0.16273184848965114 2.368312857365961 >50 0.01762538598460682 0.9591223880095889 >100 0.01387587094646456 2.333790049247174 >500 0.002012515646077111 0.9395732336597188 >1k 0.0016947500177491462 2.2922058294525502 >5k 2.1184375221864327E-4 0.9958512444387804 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 6259 0.5093570658949678 No Hit CCCATGTACTCTGCGTTGATACCAC 5933 0.4828272043385275 No Hit GTATCAACGCAGAGTACTTTTTTTT 3304 0.26887933307508766 No Hit GAGTACATGGGAAGCAGTGGTATCA 2923 0.2378735746302909 No Hit GGTATCAACGCAGAGTACTTTTTTT 2555 0.20792575545001482 No Hit CATGTACTCTGCGTTGATACCACTG 2418 0.19677670320083596 No Hit TATCAACGCAGAGTACTTTTTTTTT 2138 0.17399031904193019 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1864 0.15169221454357243 No Hit GCGTTGATACCACTGCTTCCCATGT 1774 0.14436801963535276 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1537 0.12508097304370755 No Hit GTATCAACGCAGAGTACATGGGAAG 1425 0.11596641938014525 No Hit TATCAACGCAGAGTACATGGGAAGC 1289 0.10489874707439104 No Hit ACTCTGCGTTGATACCACTGCTTCC 1229 0.10001595046891124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.137994342466334E-5 0.0 9 0.0 0.0 0.0 8.137994342466334E-5 0.0 10 0.0 0.0 0.0 8.137994342466334E-5 0.0 11 0.0 0.0 0.0 8.137994342466334E-5 0.0 12 0.0 0.0 0.0 8.137994342466334E-5 0.0 13 0.0 0.0 0.0 8.137994342466334E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGT 35 0.0021737057 16.281387 8 CGATTTC 55 1.953895E-4 13.822952 12 GGCGATT 55 1.9640467E-4 13.814511 10 TTCCGAA 65 5.4309712E-5 13.158923 16 ATTTCCG 65 5.4330478E-5 13.158386 14 TTTCCGA 60 4.0790447E-4 12.671039 15 GTATTGG 75 1.4783103E-5 12.666395 1 GCGATTT 60 4.1001203E-4 12.663301 11 CCGAATG 85 3.9226543E-6 12.299363 18 CTAGGAC 135 3.7289283E-10 11.960758 3 AGCGTAC 160 3.6379788E-12 11.874746 1 TCCGAAT 75 2.0657218E-4 11.4044 17 CGCCCTA 170 1.0913936E-11 11.180783 16 TAGGACT 200 0.0 10.922542 4 GTCCCGC 175 2.0008883E-11 10.86089 12 CGGCGAT 70 0.001494325 10.854259 9 GCCTTAA 80 3.7702048E-4 10.687271 1 CTACACT 135 5.6988938E-8 10.553181 4 TAGACAG 110 6.0569528E-6 10.361304 5 GCTACAC 130 3.8467624E-7 10.228884 3 >>END_MODULE