FastQCFastQC Report
Wed 25 May 2016
SRR1294660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228804
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT91920.7480444399595053No Hit
GGTATCAACGCAGAGTACTTTTTTT79340.6456684711312789No Hit
CCCATGTACTCTGCGTTGATACCAC66900.5444318215109977No Hit
TATCAACGCAGAGTACTTTTTTTTT65020.529132392147161No Hit
GTACATGGGAAGCAGTGGTATCAAC61250.4984521534760629No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42350.3446440604034492No Hit
GAGTACATGGGAAGCAGTGGTATCA30900.25146402518220967No Hit
CATGTACTCTGCGTTGATACCACTG27740.22574796306001607No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26010.21166923284754932No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21910.17830345604343736No Hit
GCGTTGATACCACTGCTTCCCATGT19660.15999296877288813No Hit
ACGCAGAGTACATGGGAAGCAGTGG18740.1525060139778191No Hit
GTATCAACGCAGAGTACATGGGAAG15980.130045149592612No Hit
ACTCTGCGTTGATACCACTGCTTCC14670.1193843770039811No Hit
TATCAACGCAGAGTACATGGGAAGC14580.11865195751315914No Hit
GGTATCAACGCAGAGTACATGGGAA13380.10888636430219953No Hit
GCTTCCCATGTACTCTGCGTTGATA12790.1040849476401444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCA250.005954262319.0423689
GTCTAAT759.607738E-713.94105151
GGACTAT1009.742507E-913.3307486
ACGCGTA550.00301704912.1178729
TTCCGAA752.1098617E-411.37846818
TCGCGCG1201.2353667E-711.1080489
CTGTGCG600.00577785211.1080489
TATCGCG1053.3691285E-610.8822437
GCGCGCC1252.273664E-710.627686511
CGCCCTA1803.6379788E-1110.53561716
GTCTTAG1002.382636E-510.4557881
CGTCGGT1002.4466886E-510.43068519
AGCGTCG1002.4477875E-510.43026217
GCGTCGG1002.4477875E-510.43026218
ATCGCGC1105.8764745E-610.3875968
TGGACTG1855.638867E-1110.2940145
ACTGTCC1502.3550456E-810.1567618
TTTCCGA750.002688732210.11419317
CTAAGAC856.6042383E-410.0566013
GTCCCGC1901.03682396E-109.98151812
Produced by FastQC (version 0.11.5)