FastQCFastQC Report
Wed 25 May 2016
SRR1294659_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294659_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466995
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC129200.3726570127733094No Hit
GTATCAACGCAGAGTACTTTTTTTT119510.34470773681531125No Hit
CCCATGTACTCTGCGTTGATACCAC113160.3263921638190998No Hit
GGTATCAACGCAGAGTACTTTTTTT73180.2110761624980711No Hit
TATCAACGCAGAGTACTTTTTTTTT68120.19648139094518452No Hit
GAGTACATGGGAAGCAGTGGTATCA58360.16833021103289736No Hit
CATGTACTCTGCGTTGATACCACTG41610.12001747911375701No Hit
GTATCAACGCAGAGTACATGGGAAG40110.11569096580756534No Hit
GGTATCAACGCAGAGTACATGGGAA36180.10435550094534315No Hit
GCGTTGATACCACTGCTTCCCATGT36150.10426897067921932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC700.001497006210.8529628
GCGACTA1054.0858566E-59.95731516
TCTAGAC3100.09.4974383
AGCTCCG1201.7089467E-59.4966156
TCTGTCG3500.09.4963428
TAGACAG6000.09.3387425
CTAGGAC2750.09.3247573
CTAGACA4500.09.2859814
CGGGTTC2900.09.17841918
CGTTCCG1151.1158335E-49.08358810
CCGGGTT3150.09.05210417
CGCCCTA950.00181642649.00446116
CCTAGAC3200.08.9038483
GTCTAAG3000.08.8661951
ACAGTCC4400.08.8488648
CGCCTCC2900.08.84142111
AAGCGAC1401.0793487E-58.82567414
CTGTCGC4200.08.8182879
TAGGACC2054.1691237E-98.8021224
TATACTG8750.08.7915335
Produced by FastQC (version 0.11.5)