FastQCFastQC Report
Wed 25 May 2016
SRR1294659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466995
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT298950.8622741019240006No Hit
GGTATCAACGCAGAGTACTTTTTTT198940.573811038089181No Hit
TATCAACGCAGAGTACTTTTTTTTT180160.5196430914956612No Hit
CCCATGTACTCTGCGTTGATACCAC126370.36449432433562784No Hit
GTACATGGGAAGCAGTGGTATCAAC125620.362331067682532No Hit
ACGCAGAGTACTTTTTTTTTTTTTT106200.30631714207837046No Hit
GAGTACATGGGAAGCAGTGGTATCA60150.1734931835782861No Hit
GAGTACTTTTTTTTTTTTTTTTTTT46270.13345851378499246No Hit
CATGTACTCTGCGTTGATACCACTG45390.13092029264536004No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44280.1277186727987782No Hit
GTATCAACGCAGAGTACATGGGAAG42430.1223826397211418No Hit
ACGCAGAGTACATGGGAAGCAGTGG39090.11274893675935499No Hit
GGTATCAACGCAGAGTACATGGGAA38700.11162404329974517No Hit
GCGTTGATACCACTGCTTCCCATGT37370.10778786816825522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCG456.652253E-414.8106568
GGACCGT456.652253E-414.8106566
ACAGCGT1103.7030986E-812.1178098
AGAGCGT657.879499E-411.7183226
CGCAGCG1900.011.0244738
CTAGGAC3150.010.5551943
CGTACTT1054.1946525E-59.93352613
TAGCACC2054.0017767E-119.753364
AGACTGT6450.09.4473296
CCTACAC3850.09.3763033
CAGCGTA1856.4628694E-99.26313710
TAGACTG5450.09.2590875
GTCTAGG3450.09.0949251
CGTCTCA1151.1343484E-49.06987112
GCGTACT1151.1343484E-49.06987112
GTCCTAA2850.09.0078921
ACTGTCC3700.09.006488
GTCCCGC950.00185614658.98307812
TAGGACT4050.08.9334124
ACGCAGC2252.5102054E-108.8863947
Produced by FastQC (version 0.11.5)