##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294659_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3466995 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.333601000289878 31.0 31.0 34.0 28.0 34.0 2 31.474109423290198 31.0 31.0 34.0 28.0 34.0 3 31.6212685625448 31.0 31.0 34.0 28.0 34.0 4 35.18893306739698 37.0 35.0 37.0 32.0 37.0 5 35.321218519207555 37.0 35.0 37.0 32.0 37.0 6 34.95521279955697 37.0 35.0 37.0 32.0 37.0 7 34.79829246941516 37.0 35.0 37.0 32.0 37.0 8 34.85386220631988 37.0 35.0 37.0 32.0 37.0 9 36.29082332106046 39.0 37.0 39.0 32.0 39.0 10 36.084064441973524 39.0 35.0 39.0 30.0 39.0 11 36.230887843795564 39.0 37.0 39.0 31.0 39.0 12 36.170907370792285 39.0 35.0 39.0 31.0 39.0 13 36.02091263471681 39.0 35.0 39.0 30.0 39.0 14 37.12917872682251 40.0 37.0 41.0 31.0 41.0 15 37.16958893797078 40.0 37.0 41.0 31.0 41.0 16 37.218884076844645 40.0 37.0 41.0 31.0 41.0 17 37.28147920605596 40.0 37.0 41.0 31.0 41.0 18 37.30587064590517 40.0 37.0 41.0 31.0 41.0 19 37.338005390835576 40.0 37.0 41.0 31.0 41.0 20 37.38973087645064 40.0 37.0 41.0 31.0 41.0 21 37.24132570136386 40.0 37.0 41.0 30.0 41.0 22 37.246883540357 40.0 37.0 41.0 31.0 41.0 23 37.26227381348978 40.0 37.0 41.0 31.0 41.0 24 37.18547618326534 40.0 37.0 41.0 30.0 41.0 25 36.170117637896794 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4967196845671289 1101 2 0.4333785474501397 1101 3 0.4201159962927754 1101 4 0.27977501218556 1101 5 0.26700412456093403 1101 6 0.35582884785648616 1101 7 0.4087645715135153 1101 8 0.34842325381910655 1101 9 0.45283469815292676 1101 10 0.5329385070105062 1101 11 0.5394249877903334 1101 12 0.6439117421608671 1101 13 0.5975967366822204 1101 14 0.7635423693962196 1101 15 0.7514958063256287 1101 16 0.7468988036472552 1101 17 0.744806052841021 1101 18 0.7524158576274687 1101 19 0.8367305587158569 1101 20 0.8053787656283404 1101 21 1.1051710724403492 1101 22 1.0135383738103911 1101 23 1.0275054836504722 1101 24 1.0872261172371793 1101 25 1.0235725723064562 1102 1 0.3840394132223999 1102 2 0.41376337592965484 1102 3 0.2885300720220023 1102 4 0.3157321335137766 1102 5 0.3018543113009855 1102 6 0.35644166456685866 1102 7 0.43195503834358817 1102 8 0.44785331667198847 1102 9 0.6116470278548718 1102 10 0.6928960152354051 1102 11 0.6296946030732826 1102 12 0.6741374437788323 1102 13 0.6996797385012528 1102 14 0.8298787252484559 1102 15 0.8881256955207348 1102 16 0.8476858352983498 1102 17 0.7761285708965673 1102 18 0.7268513695724579 1102 19 0.7738633021654664 1102 20 0.7714813565434895 1102 21 1.0679918835594364 1102 22 1.0371070900934356 1102 23 0.9455354708178021 1102 24 1.0846164589944252 1102 25 1.0046195379087521 1103 1 0.2875453808805659 1103 2 0.2603938607097902 1103 3 0.21362572319231177 1103 4 0.18372898034472485 1103 5 0.19801252105396117 1103 6 0.2703404281189563 1103 7 0.3273427722470501 1103 8 0.35813131435361356 1103 9 0.2548001744968431 1103 10 0.5322327516157941 1103 11 0.498362310711542 1103 12 0.49491395928050963 1103 13 0.6188501435333293 1103 14 0.7636804397701198 1103 15 0.6955768782672749 1103 16 0.623801644227953 1103 17 0.5946858783994671 1103 18 0.6154010742850105 1103 19 0.6766013711911825 1103 20 0.6507054573328475 1103 21 0.8697852904779211 1103 22 0.8253508343192095 1103 23 0.8582230544022735 1103 24 1.010781767299619 1103 25 0.8857925750638174 1104 1 0.18776864249830183 1104 2 0.16804370019244175 1104 3 0.11725900882899865 1104 4 0.1542028587129991 1104 5 0.11721123534363187 1104 6 0.10238505395060571 1104 7 0.1838739519235375 1104 8 0.11621369585876096 1104 9 -0.137140630024021 1104 10 0.3205411124036033 1104 11 0.36151532063488645 1104 12 0.46121412845823784 1104 13 -0.047342677318674475 1104 14 0.2730504118514716 1104 15 0.40816469583192116 1104 16 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0.1861123357082981 2207 2 0.2160029782618551 2207 3 0.1929112159631572 2207 4 0.14889800047431123 2207 5 0.15331061826569936 2207 6 0.2684603634904619 2207 7 0.2249696626347628 2207 8 0.2462486325452815 2207 9 0.3061160034232344 2207 10 0.5431228112155324 2207 11 0.3970637654523159 2207 12 0.33210706174802596 2207 13 0.407779558492976 2207 14 0.361531867910756 2207 15 0.2856321089003586 2207 16 0.47015513312999957 2207 17 0.5014246024664715 2207 18 0.4459426848743533 2207 19 0.5398368051504363 2207 20 0.45319025755764386 2207 21 0.7817694683193608 2207 22 0.6218419286259191 2207 23 0.5561510489743071 2207 24 0.7532282907338796 2207 25 0.7075920553972779 2208 1 0.15063767471451683 2208 2 0.15765928474043278 2208 3 0.1805763105949687 2208 4 0.12660764083111076 2208 5 0.09969722814560811 2208 6 0.1815338737109684 2208 7 0.22614820609662445 2208 8 0.14304217377314643 2208 9 0.30532175362125 2208 10 0.3353335922254246 2208 11 0.06856088489902845 2208 12 -0.11989180829828427 2208 13 0.08103095798316673 2208 14 -0.4214097089948723 2208 15 0.18828522341665632 2208 16 0.2748528435699953 2208 17 0.1983874316079124 2208 18 0.2971520756051902 2208 19 0.42141587318884177 2208 20 0.36951634661627253 2208 21 0.6626039422521472 2208 22 0.6296084898162988 2208 23 0.6682178417384179 2208 24 0.7442729894683993 2208 25 0.7277043219189707 2209 1 0.06643194511585193 2209 2 0.03789740976766609 2209 3 0.09022524686298539 2209 4 -0.5077926556060106 2209 5 -0.05870142381698429 2209 6 0.11260688734044066 2209 7 0.13794397307371042 2209 8 0.15299630116835772 2209 9 0.1699178840296014 2209 10 0.26709141717002893 2209 11 0.285206852398872 2209 12 0.2759786979272505 2209 13 0.37872205819418525 2209 14 0.38996400683401333 2209 15 -0.1776493909332828 2209 16 -0.20380991963486395 2209 17 0.17901213206292255 2209 18 -0.16693302455226444 2209 19 0.2639022355224583 2209 20 0.2583116151344953 2209 21 0.5846718589699478 2209 22 0.47702054100846425 2209 23 0.48177560280674214 2209 24 0.6232887111085148 2209 25 0.4846809271303627 2210 1 -0.20676285951141082 2210 2 -0.14451246854595468 2210 3 -0.10548627229271546 2210 4 -0.0515229118664422 2210 5 -0.07055001444729925 2210 6 -0.503260938906962 2210 7 -0.23465941462525564 2210 8 -0.31607875609784486 2210 9 -0.09005992974285704 2210 10 0.048306241600208466 2210 11 -0.009005993762727371 2210 12 -0.008665705361146081 2210 13 0.06080701327748983 2210 14 0.1431216293994666 2210 15 -0.02564643951355805 2210 16 0.039209875594707455 2210 17 -0.9110408874773839 2210 18 -1.523793659192478 2210 19 -0.3392795843146885 2210 20 -0.12712733268072895 2210 21 -0.34338590938609315 2210 22 -0.32939365801531295 2210 23 -0.19503592512957368 2210 24 0.21812162247325517 2210 25 0.20930703274942886 2211 1 -0.22278452279817884 2211 2 -0.21458049561137926 2211 3 -0.15783882329697718 2211 4 -0.11506734136197139 2211 5 -0.09395909544257108 2211 6 -0.1542622073314064 2211 7 -0.1852002483983597 2211 8 -0.13553739630921058 2211 9 -0.11131822731711338 2211 10 -0.06722479459906339 2211 11 -0.3777916548359528 2211 12 -0.2640910209918488 2211 13 -0.1702245245609859 2211 14 -0.24733307039961971 2211 15 -0.3010518777443636 2211 16 -0.24976545898110203 2211 17 -0.3587891774992542 2211 18 -0.46609037557259825 2211 19 -0.22668146958856283 2211 20 -0.25656981181330707 2211 21 0.08228343468537247 2211 22 -0.06523107474021117 2211 23 -0.6528226228382863 2211 24 -1.4176504965490722E-4 2211 25 -0.7146015277898243 2212 1 -0.13795480431911855 2212 2 -0.22538245312713912 2212 3 -0.1290727939249301 2212 4 -0.10186435386884085 2212 5 -0.10831196047456615 2212 6 -0.08003119812121184 2212 7 -0.16160179988326462 2212 8 -0.29577803478343156 2212 9 0.00892743997971479 2212 10 -0.34111286604559865 2212 11 -0.09846872992372369 2212 12 -0.20938162174989827 2212 13 -0.17085918059883198 2212 14 -0.1244793998247502 2212 15 -0.060516308137081865 2212 16 -0.2276984732014924 2212 17 -0.4830870048616376 2212 18 -0.33790126390480424 2212 19 -0.32303091688370955 2212 20 -0.45032266380761143 2212 21 0.08309968709501447 2212 22 -0.2669066504938371 2212 23 -0.16960492138272087 2212 24 -0.2814852988219201 2212 25 -0.02405292049430585 2213 1 -0.18994312188335627 2213 2 -0.23732943196131018 2213 3 -0.13849962680495764 2213 4 -0.04524002267827143 2213 5 -0.04381352641503611 2213 6 -0.2372586798876739 2213 7 -0.3595893267274448 2213 8 -0.32815492114913525 2213 9 -0.2873020462477882 2213 10 -0.2899870437897363 2213 11 -0.061556553740992115 2213 12 0.017443150775008576 2213 13 0.07038340401608423 2213 14 -0.716578112973167 2213 15 -0.27264236741819303 2213 16 -0.37072790391643906 2213 17 -0.2713283585667412 2213 18 -0.31483956532557045 2213 19 -0.3235053012261204 2213 20 -0.3804896354594831 2213 21 -0.12836160458292056 2213 22 -0.41887737000616454 2213 23 -0.5140282704207664 2213 24 -0.6875996918822835 2213 25 -0.4978741729319225 2214 1 -0.25034192379895615 2214 2 -0.2236138196081363 2214 3 -0.21832562466263994 2214 4 -0.20246107475485786 2214 5 -0.15931920960110801 2214 6 -0.16061397913545505 2214 7 -0.20071365899814708 2214 8 -0.21763237197103535 2214 9 -0.19957155631019674 2214 10 -0.22613585995255647 2214 11 -0.12495832024092124 2214 12 -0.3101927492672871 2214 13 -0.3523183646936161 2214 14 -0.2963481880782979 2214 15 -0.12001105168472748 2214 16 -0.2717101284285519 2214 17 -0.33128268605736366 2214 18 -0.3758273491601898 2214 19 -0.6095875916049636 2214 20 -0.5581010729320326 2214 21 -0.5681821322843561 2214 22 -0.7029266696439507 2214 23 -1.08626660718312 2214 24 -0.6714753305356211 2214 25 -0.869878331956464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 9.0 9 34.0 10 84.0 11 213.0 12 555.0 13 1341.0 14 2917.0 15 5188.0 16 7948.0 17 10239.0 18 12406.0 19 13626.0 20 14764.0 21 15865.0 22 16705.0 23 18364.0 24 20352.0 25 22840.0 26 26003.0 27 29100.0 28 34636.0 29 43126.0 30 55368.0 31 72719.0 32 98557.0 33 134600.0 34 186556.0 35 260875.0 36 378357.0 37 608855.0 38 1374792.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.261152050369724 21.22712107511459 12.610388755504282 23.901338119011406 2 14.563072632063212 21.51113572416459 40.32676712830563 23.599024515466564 3 18.173555229956218 27.27527941419846 29.532254696472005 25.01891065937332 4 11.50919317347229 17.642677509970884 38.54979844769823 32.2983308688586 5 12.396125799576021 39.086991793923744 34.44131518787656 14.075567218623675 6 30.240310028322337 38.978953544792155 17.34822395473042 13.43251247215509 7 25.7450173502724 34.20415506423697 22.50864628639547 17.542181299095155 8 24.629155538015127 37.35557274979099 20.46093821672699 17.554333495466896 9 25.012633458937223 16.65499465818129 22.177229545910798 36.15514233697069 10 14.68846214096117 30.897065376867527 33.00100968246977 21.413462799701524 11 31.83799062144541 26.357017161306 22.23529048437778 19.569701732870804 12 21.113473737161634 28.606943505419167 31.44419074737695 18.835392010042252 13 26.984161779386344 23.928978224069102 26.881998319581264 22.204861676963294 14 20.53455538518516 23.37223200986687 29.047729477962804 27.04548312698517 15 23.17748361600726 30.017211088359772 25.191731870453317 21.61357342517965 16 20.751124607296013 31.521626264642393 26.628675957343955 21.09857317071764 17 18.911189661363803 30.340914826816885 29.29857124108918 21.449324270730127 18 19.199245452814843 29.549261893636537 31.028849315542633 20.222643338005987 19 19.896595586347733 28.49811218441463 31.90018950264929 19.705102726588347 20 21.813522808132827 27.674115882176658 30.8991351856814 19.613226124009117 21 22.038713696895577 28.328002099804152 29.765185362519077 19.868098840781194 22 20.383929887452215 28.042278900095564 31.212907963724184 20.360883248728037 23 20.123011568244625 28.4490210675482 30.896128660270534 20.531838703936643 24 20.080300086962918 28.144228647575204 31.07192251503103 20.703548750430848 25 20.61234632283864 27.981206411326227 30.054887224334404 21.35156004150073 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2122.0 1 2122.0 2 2384.0 3 2646.0 4 2646.0 5 2646.0 6 5876.0 7 9106.0 8 9106.0 9 9106.0 10 12901.5 11 16697.0 12 16697.0 13 16697.0 14 24049.0 15 31401.0 16 31401.0 17 31401.0 18 51047.5 19 70694.0 20 70694.0 21 70694.0 22 104478.5 23 138263.0 24 138263.0 25 138263.0 26 182058.5 27 225854.0 28 225854.0 29 225854.0 30 269196.5 31 312539.0 32 312539.0 33 312539.0 34 341153.5 35 369768.0 36 369768.0 37 369768.0 38 382855.0 39 395942.0 40 395942.0 41 395942.0 42 394778.0 43 393614.0 44 393614.0 45 393614.0 46 408922.0 47 424230.0 48 424230.0 49 424230.0 50 397441.0 51 370652.0 52 370652.0 53 370652.0 54 325670.5 55 280689.0 56 280689.0 57 280689.0 58 240052.5 59 199416.0 60 199416.0 61 199416.0 62 161919.0 63 124422.0 64 124422.0 65 124422.0 66 92995.0 67 61568.0 68 61568.0 69 61568.0 70 43478.5 71 25389.0 72 25389.0 73 25389.0 74 17036.0 75 8683.0 76 8683.0 77 8683.0 78 5613.0 79 2543.0 80 2543.0 81 2543.0 82 1578.0 83 613.0 84 613.0 85 613.0 86 364.0 87 115.0 88 115.0 89 115.0 90 68.0 91 21.0 92 21.0 93 21.0 94 13.5 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0959620651313313 2 0.0 3 0.13446803355643722 4 0.011566212238552406 5 0.022584399458320532 6 0.01364293862552441 7 0.022122904705660088 8 0.020680733603596197 9 3.17277642454056E-4 10 0.3874825317025263 11 0.065820689098196 12 0.0012402671477749463 13 5.7686844082555644E-5 14 0.001124893459609835 15 0.001269110569816224 16 2.0190395428894474E-4 17 0.0 18 4.3265133061916734E-4 19 7.210855510319455E-4 20 2.3074737633022258E-4 21 1.442171102063891E-4 22 0.0032304632686231158 23 1.1537368816511129E-4 24 0.0 25 0.001961352698806892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3466995.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.30228050852244 #Duplication Level Percentage of deduplicated Percentage of total 1 90.75233350709811 71.06114675075777 2 7.254808303810554 11.36136069681064 3 1.080515925958202 2.5382058338491507 4 0.2966574890172947 0.929158316799445 5 0.13641822616583543 0.5340929105856151 6 0.09842922177308358 0.4624339520106933 7 0.061062194552617494 0.33469163604275187 8 0.04460857889290956 0.27943627660473264 9 0.03547526782673927 0.25000149352358697 >10 0.204688550480028 3.020016871484242 >50 0.019424139955093855 1.0340842716642535 >100 0.012806886460329056 1.9980057404200293 >500 0.0010717271031478609 0.5891380433346614 >1k 0.0014412881594893271 2.435551461673647 >5k 3.6956106653572465E-5 0.1740589078959079 >10k+ 2.217366399214348E-4 2.9986168365429022 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 29895 0.8622741019240006 No Hit GGTATCAACGCAGAGTACTTTTTTT 19894 0.573811038089181 No Hit TATCAACGCAGAGTACTTTTTTTTT 18016 0.5196430914956612 No Hit CCCATGTACTCTGCGTTGATACCAC 12637 0.36449432433562784 No Hit GTACATGGGAAGCAGTGGTATCAAC 12562 0.362331067682532 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10620 0.30631714207837046 No Hit GAGTACATGGGAAGCAGTGGTATCA 6015 0.1734931835782861 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4627 0.13345851378499246 No Hit CATGTACTCTGCGTTGATACCACTG 4539 0.13092029264536004 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4428 0.1277186727987782 No Hit GTATCAACGCAGAGTACATGGGAAG 4243 0.1223826397211418 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3909 0.11274893675935499 No Hit GGTATCAACGCAGAGTACATGGGAA 3870 0.11162404329974517 No Hit GCGTTGATACCACTGCTTCCCATGT 3737 0.10778786816825522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.8843422041277822E-5 0.0 0.0 0.0 3 0.0 2.8843422041277822E-5 0.0 0.0 0.0 4 0.0 2.8843422041277822E-5 0.0 2.8843422041277822E-5 0.0 5 0.0 2.8843422041277822E-5 0.0 2.8843422041277822E-5 0.0 6 0.0 2.8843422041277822E-5 0.0 2.8843422041277822E-5 0.0 7 0.0 2.8843422041277822E-5 0.0 2.8843422041277822E-5 0.0 8 0.0 2.8843422041277822E-5 0.0 2.8843422041277822E-5 0.0 9 0.0 2.8843422041277822E-5 0.0 1.1537368816511129E-4 0.0 10 0.0 2.8843422041277822E-5 0.0 1.1537368816511129E-4 0.0 11 0.0 2.8843422041277822E-5 0.0 1.1537368816511129E-4 0.0 12 0.0 2.8843422041277822E-5 0.0 1.1537368816511129E-4 0.0 13 0.0 2.8843422041277822E-5 0.0 1.442171102063891E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGTCG 45 6.652253E-4 14.810656 8 GGACCGT 45 6.652253E-4 14.810656 6 ACAGCGT 110 3.7030986E-8 12.117809 8 AGAGCGT 65 7.879499E-4 11.718322 6 CGCAGCG 190 0.0 11.024473 8 CTAGGAC 315 0.0 10.555194 3 CGTACTT 105 4.1946525E-5 9.933526 13 TAGCACC 205 4.0017767E-11 9.75336 4 AGACTGT 645 0.0 9.447329 6 CCTACAC 385 0.0 9.376303 3 CAGCGTA 185 6.4628694E-9 9.263137 10 TAGACTG 545 0.0 9.259087 5 GTCTAGG 345 0.0 9.094925 1 CGTCTCA 115 1.1343484E-4 9.069871 12 GCGTACT 115 1.1343484E-4 9.069871 12 GTCCTAA 285 0.0 9.007892 1 ACTGTCC 370 0.0 9.00648 8 GTCCCGC 95 0.0018561465 8.983078 12 TAGGACT 405 0.0 8.933412 4 ACGCAGC 225 2.5102054E-10 8.886394 7 >>END_MODULE