FastQCFastQC Report
Wed 25 May 2016
SRR1294658_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294658_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175386
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC68270.5808304676080879No Hit
CCCATGTACTCTGCGTTGATACCAC61610.5241682306918748No Hit
GTATCAACGCAGAGTACTTTTTTTT37300.3173425581043164No Hit
GAGTACATGGGAAGCAGTGGTATCA33080.28143945903728645No Hit
GGTATCAACGCAGAGTACTTTTTTT26400.22460706525345717No Hit
CATGTACTCTGCGTTGATACCACTG23590.20070002535337328No Hit
TATCAACGCAGAGTACTTTTTTTTT22220.18904427992165979No Hit
GCGTTGATACCACTGCTTCCCATGT19710.16768959303581973No Hit
GTATCAACGCAGAGTACATGGGAAG19030.16190425953686705No Hit
ACGCAGAGTACATGGGAAGCAGTGG17760.15109929844323483No Hit
GCTTCCCATGTACTCTGCGTTGATA15500.13187157240259795No Hit
GGTATCAACGCAGAGTACATGGGAA15500.13187157240259795No Hit
ATTCCATTCCATTCCATTCCATTCC14710.12515037613175586No Hit
TATCAACGCAGAGTACATGGGAAGC13600.1157066699790537No Hit
ACTCTGCGTTGATACCACTGCTTCC13190.11221845419292044No Hit
GTGGTATCAACGCAGAGTACATGGG13190.11221845419292044No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11780.10022239502597444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT350.00216231516.29411315
CCCGCCC759.610867E-713.94051914
GTCCCGC707.220373E-613.57842712
CCGCCCT655.4239696E-513.16062915
GTATAGG1457.2759576E-1212.4472711
CGCCCTA701.08522654E-412.22058516
CCGTACA550.003055853512.09714517
CCTACAC951.0432705E-611.9959673
ACGTGTC802.8748353E-511.871018
CGTGTCC802.8748353E-511.871019
TGTCCCG855.3383075E-511.17271411
TAGACAG1658.0035534E-1110.9357185
TAGGATG1158.845873E-710.7355214
TCCCGCC909.4600226E-510.56113
GTCCTAC909.517391E-510.5547051
GGCTAGA1355.685797E-810.5547041
ACTATAC1451.4484613E-810.4792363
TATACTG4200.010.4012655
GAACAGT1659.331416E-1010.3601546
GTCTATA1752.3464963E-1010.3134541
Produced by FastQC (version 0.11.5)