FastQCFastQC Report
Wed 25 May 2016
SRR1294658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175386
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT91640.7796587674176824No Hit
CCCATGTACTCTGCGTTGATACCAC71130.6051628996772124No Hit
GGTATCAACGCAGAGTACTTTTTTT70490.5997178799134922No Hit
GTACATGGGAAGCAGTGGTATCAAC67480.5741092713372458No Hit
TATCAACGCAGAGTACTTTTTTTTT58320.4961774259690009No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41080.3495022060837886No Hit
GAGTACATGGGAAGCAGTGGTATCA33720.28688447880100665No Hit
CATGTACTCTGCGTTGATACCACTG27420.2332850655018862No Hit
ACGCAGAGTACATGGGAAGCAGTGG21760.1851306719664859No Hit
GCGTTGATACCACTGCTTCCCATGT20420.1737301618361968No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19990.1700717891824473No Hit
GGTATCAACGCAGAGTACATGGGAA18280.15552337700125746No Hit
GTATCAACGCAGAGTACATGGGAAG18170.15458751422936803No Hit
GCTTCCCATGTACTCTGCGTTGATA16950.14420794530477646No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16890.1436974747019277No Hit
TATCAACGCAGAGTACATGGGAAGC15310.13025508216024353No Hit
ATTCCATTCCATTCCATTCCATTCC14920.12693702324172654No Hit
ACTCTGCGTTGATACCACTGCTTCC14700.12506529769794775No Hit
GTGGTATCAACGCAGAGTACATGGG14060.11962027793422755No Hit
GAATGGAATGGAATGGAATGGAATG11970.10183888526832886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG655.3449585E-513.1812245
GCTCCGC550.003020268612.1160747
TCTATAC1604.5474735E-1111.2830473
AAGACTG1354.5693014E-911.2826945
ACACGGT855.2085416E-511.1997336
GTCCTGT855.2517615E-511.1906531
AGCTCCG600.005783979311.1064016
CCAGGAC1502.1227606E-910.7683823
TAGGACT1158.57488E-710.7614824
ACTGTCC803.6974513E-410.7097448
GTATAGA2550.010.444611
TAGGACA1951.4551915E-1110.2520634
GCCCTAA750.00261904410.1461921
TATACTG3700.010.0343555
GTCCTGC951.6221497E-410.0126891
TCCTAAG1909.822543E-1110.0015932
TCTGTCG1151.00041725E-59.9336778
GACCATG1252.4996643E-69.9005637
TGGACAG1356.2535764E-79.8723575
GACTGTG1659.857104E-99.808257
Produced by FastQC (version 0.11.5)