##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294658_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1175386 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.266280183701355 31.0 31.0 34.0 28.0 34.0 2 31.40890226699995 31.0 31.0 34.0 28.0 34.0 3 31.560027088973325 31.0 31.0 34.0 28.0 34.0 4 35.12002950520085 37.0 35.0 37.0 32.0 37.0 5 35.26465263326261 37.0 35.0 37.0 32.0 37.0 6 34.87830210671218 37.0 35.0 37.0 32.0 37.0 7 34.72629331981153 37.0 35.0 37.0 32.0 37.0 8 34.778611451897504 37.0 35.0 37.0 32.0 37.0 9 36.19382228476432 39.0 37.0 39.0 31.0 39.0 10 35.99978049764078 39.0 35.0 39.0 30.0 39.0 11 36.1381384498369 39.0 35.0 39.0 31.0 39.0 12 36.08394859220716 39.0 35.0 39.0 30.0 39.0 13 35.92343706663173 39.0 35.0 39.0 30.0 39.0 14 37.03054145616844 40.0 36.0 41.0 30.0 41.0 15 37.08238144745641 40.0 36.0 41.0 30.0 41.0 16 37.103326056291294 40.0 36.0 41.0 31.0 41.0 17 37.17961333553403 40.0 37.0 41.0 31.0 41.0 18 37.19044977564817 40.0 37.0 41.0 31.0 41.0 19 37.226417534324895 40.0 37.0 41.0 30.0 41.0 20 37.28877747395324 40.0 37.0 41.0 31.0 41.0 21 37.1352696050489 40.0 37.0 41.0 30.0 41.0 22 37.144708206495565 40.0 37.0 41.0 30.0 41.0 23 37.164078013520665 40.0 37.0 41.0 30.0 41.0 24 37.090552380239345 40.0 36.0 41.0 30.0 41.0 25 36.03601965652135 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.49153912055304616 1101 2 0.5539550335996957 1101 3 0.4956181579873764 1101 4 0.4850610242416451 1101 5 0.3709662511164211 1101 6 0.41407143734657126 1101 7 0.4964540514934441 1101 8 0.5655300315883736 1101 9 0.7048439566242806 1101 10 0.6156915230994926 1101 11 0.6614488785261798 1101 12 0.6253196160694543 1101 13 0.6348887366438447 1101 14 0.8391532499397414 1101 15 0.8821915602409973 1101 16 0.939886380997315 1101 17 0.7689043506014954 1101 18 0.7844148617348381 1101 19 1.0380385899895117 1101 20 0.9342976759254427 1101 21 1.134357463458251 1101 22 1.0952543490005127 1101 23 0.9557046271203333 1101 24 1.1390337738010743 1101 25 1.1334191320577034 1102 1 0.3924260385131326 1102 2 0.3773554137073951 1102 3 0.371322941009236 1102 4 0.32453679237594457 1102 5 0.2819814554243081 1102 6 0.35757001194270543 1102 7 0.42868401665023725 1102 8 0.3952021886995567 1102 9 0.5478119642264332 1102 10 0.6918001705495982 1102 11 0.6448983558780981 1102 12 0.7884254127118382 1102 13 0.599712936833896 1102 14 0.7897946816787353 1102 15 0.8513315665761283 1102 16 0.7865525197366239 1102 17 0.7378859787294658 1102 18 0.8111649409239448 1102 19 0.9580100819122279 1102 20 0.8683806661059918 1102 21 1.074585528076561 1102 22 1.0264390179900573 1102 23 0.9081040569587913 1102 24 1.1423755539721228 1102 25 0.9008328159348054 1103 1 0.2078374563635741 1103 2 0.20548594171597045 1103 3 0.18367351726224967 1103 4 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16 0.44568940288679215 1104 17 0.5380264804472148 1104 18 0.4423441179896699 1104 19 0.5894928403224924 1104 20 0.6572482314141226 1104 21 0.7607632711399575 1104 22 0.6300456864059996 1104 23 0.6860404055617053 1104 24 0.7320083273326361 1104 25 0.77333704166508 1105 1 0.02838343060023618 1105 2 -0.02631980965041336 1105 3 -0.16110757159616895 1105 4 0.01600066627906216 1105 5 0.012898064427204758 1105 6 -0.0767047717416105 1105 7 -0.10280652547084657 1105 8 -0.11206574269178304 1105 9 0.21002576311561683 1105 10 0.21019465809728644 1105 11 0.1305217063370563 1105 12 -0.20160921616822236 1105 13 -0.5716785868980594 1105 14 -0.8818792924674881 1105 15 -0.313497769076001 1105 16 -0.13108907288567906 1105 17 0.054465435322427425 1105 18 -0.6733010517399975 1105 19 -0.6552686086200978 1105 20 -0.5475731130652406 1105 21 -0.053101960824442074 1105 22 0.09412914003313233 1105 23 -0.014708904223802222 1105 24 0.26065415265065184 1105 25 0.011260211634819939 1106 1 -0.2966968981707794 1106 2 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25 -0.2557950728860092 2207 1 0.19048326138169003 2207 2 0.2546840547047111 2207 3 0.20357842451202757 2207 4 0.18185338753731628 2207 5 0.12851305625560627 2207 6 0.2432788924741942 2207 7 0.2956551139979453 2207 8 0.2623940896030632 2207 9 0.3296503294049984 2207 10 0.4398118354488574 2207 11 0.466862297793341 2207 12 0.22241176811841257 2207 13 0.291489190211216 2207 14 0.39311438316556746 2207 15 0.4160648814754566 2207 16 0.3544359163387867 2207 17 0.3985013532755417 2207 18 0.5255392707915902 2207 19 0.585722785047821 2207 20 0.4214808877552585 2207 21 0.9097906970491962 2207 22 0.6769755651151641 2207 23 0.6772794025595559 2207 24 0.6732376769514303 2207 25 0.7743598116974368 2208 1 0.04693808176557113 2208 2 0.08010392647094022 2208 3 0.11446820202953845 2208 4 0.11218289446408392 2208 5 0.09115578426953874 2208 6 0.14962611543382565 2208 7 0.199210989399063 2208 8 0.11768505998796996 2208 9 0.2641996249007619 2208 10 0.26187402975643437 2208 11 -0.10409496620243175 2208 12 -0.34783390375964274 2208 13 -0.13337458484366493 2208 14 -0.6686012074304841 2208 15 0.009138274290954485 2208 16 0.15617489257483896 2208 17 0.08156723065495441 2208 18 0.2335983099827743 2208 19 0.32525623925193514 2208 20 0.24559778020917378 2208 21 0.6270282407264247 2208 22 0.5727219754825015 2208 23 0.659378856888786 2208 24 0.6941431704741206 2208 25 0.6142906605737366 2209 1 0.014872282003050685 2209 2 -0.08123292081684497 2209 3 0.08094275964777253 2209 4 -0.5296968780942493 2209 5 -0.09960560470784685 2209 6 0.061232784224309 2209 7 0.11558466563256786 2209 8 0.20368812987126006 2209 9 0.36050321511961414 2209 10 0.30051594428238815 2209 11 0.3866693188912578 2209 12 0.35944017503403103 2209 13 0.4600937075096212 2209 14 0.35779399660091116 2209 15 -0.2918299939280331 2209 16 -0.24608793963383846 2209 17 0.04380766623084753 2209 18 -0.06823300089600792 2209 19 0.3269263329895651 2209 20 0.31935413959789827 2209 21 0.5835401582450288 2209 22 0.45702405972087234 2209 23 0.48151290845896 2209 24 0.5046811723469418 2209 25 0.5962459252304981 2210 1 -0.22486218820550619 2210 2 -0.17668903464298324 2210 3 -0.05270956553032846 2210 4 0.045337514054210715 2210 5 0.004625144875191722 2210 6 -0.48111602699483313 2210 7 -0.15397037390383872 2210 8 -0.2206915343538256 2210 9 0.05916185411363273 2210 10 0.14939769533790326 2210 11 0.12725187483507483 2210 12 0.1985374382704137 2210 13 0.1960564680945609 2210 14 0.17211744142604601 2210 15 0.07506087519180937 2210 16 0.23421662916703667 2210 17 -0.8243161222020987 2210 18 -1.3295597357166358 2210 19 -0.24837387573828806 2210 20 -0.07653708531823611 2210 21 -0.29727005355682934 2210 22 -0.13309788789192822 2210 23 -0.09612856538015535 2210 24 0.33746390269367765 2210 25 0.3300620780239498 2211 1 -0.2440498288863857 2211 2 -0.2294745907093514 2211 3 -0.18377134421429986 2211 4 -0.1153724803334768 2211 5 -0.1433356913588284 2211 6 -0.004882139993299006 2211 7 -0.161107782927715 2211 8 -0.10077059924763887 2211 9 -0.09301623668635983 2211 10 -0.013112230687085003 2211 11 -0.3316269545208854 2211 12 -0.22477114468130566 2211 13 -0.010368282187549482 2211 14 -0.13127748301675268 2211 15 -0.18413581085921749 2211 16 -0.25176733843680665 2211 17 -0.3272364632394229 2211 18 -0.5417283333558771 2211 19 -0.23054042846712264 2211 20 -0.3100757430808372 2211 21 0.01668937064415843 2211 22 -0.19174968741309328 2211 23 -0.6378408196177432 2211 24 0.0430984891441355 2211 25 -0.7305055189525689 2212 1 -0.1577701535767595 2212 2 -0.41685389395110306 2212 3 -0.20216150694571766 2212 4 -0.19403377626262142 2212 5 -0.08773632735027803 2212 6 -0.05340384609830551 2212 7 -0.18696102854401175 2212 8 -0.6264275114705242 2212 9 -0.30608544259824555 2212 10 -0.6736131705125104 2212 11 -0.35167146748532474 2212 12 -0.4702224861855413 2212 13 -0.2793868727069011 2212 14 -0.2721531299513771 2212 15 -0.031035191604381396 2212 16 -0.205121259954943 2212 17 -0.7247793990995532 2212 18 -0.44682447234704625 2212 19 -0.5507835278311362 2212 20 -0.5598818236225895 2212 21 -0.24211022767702417 2212 22 -0.38501496923197465 2212 23 -0.15759083249206896 2212 24 -0.3770525588255893 2212 25 -0.10867501582411165 2213 1 -0.16313513950528602 2213 2 -0.11884400219903668 2213 3 -0.13559509793997648 2213 4 -0.11820143539163297 2213 5 -0.027333720566808495 2213 6 -0.277676525021775 2213 7 -0.357793719200032 2213 8 -0.2959183761812554 2213 9 -0.4046264175966243 2213 10 -0.21244225880415257 2213 11 -0.12641559762895582 2213 12 -0.013821925855204142 2213 13 -0.10293950018247244 2213 14 -0.5938084897116056 2213 15 -0.3338516775206912 2213 16 -0.3956544334828749 2213 17 -0.2144670621394127 2213 18 -0.2432809814250234 2213 19 -0.23063519369287633 2213 20 -0.3021189138157112 2213 21 0.0941492892150606 2213 22 -0.31115975663432494 2213 23 -0.48841351002482725 2213 24 -0.7549811655938825 2213 25 -0.3962105316474549 2214 1 -0.3417108676528322 2214 2 -0.17453956469251608 2214 3 -0.18315242275517818 2214 4 -0.08697275412321659 2214 5 -0.007450505971846155 2214 6 -0.060547612518981 2214 7 -0.22705399209763044 2214 8 -0.1790611841497025 2214 9 -0.36908159887885716 2214 10 -0.12170527833467304 2214 11 -0.14611823002692148 2214 12 -0.27484078398715894 2214 13 -0.3591811789102408 2214 14 -0.2909217606749692 2214 15 -0.1774536725749627 2214 16 -0.31213984912958637 2214 17 -0.46090647168467314 2214 18 -0.30554126413202454 2214 19 -0.607354626031622 2214 20 -0.5045899025054723 2214 21 -0.5026053142933193 2214 22 -0.7191533502022054 2214 23 -1.0574057186833201 2214 24 -0.6682125346387977 2214 25 -1.0782806367775066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 15.0 10 36.0 11 74.0 12 193.0 13 496.0 14 1034.0 15 1943.0 16 2989.0 17 3779.0 18 4403.0 19 4907.0 20 5351.0 21 5728.0 22 6141.0 23 6606.0 24 7330.0 25 8074.0 26 9408.0 27 10467.0 28 12256.0 29 15495.0 30 19207.0 31 25551.0 32 34046.0 33 46386.0 34 63341.0 35 88608.0 36 127847.0 37 204380.0 38 459293.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.769740427851204 22.3630603624472 11.97736408230004 23.889835127401554 2 14.2434059959877 22.194496105960084 39.97861128174064 23.58348661631158 3 18.13229041520752 27.744519141467073 30.021045815120605 24.1021446282048 4 11.56727752633742 18.09502658630786 38.44306088430425 31.89463500305047 5 12.205883604367177 39.17370844076826 34.844144903117105 13.776263051747467 6 29.976310374932563 39.286911652334325 17.302786424074924 13.433991548658183 7 24.8542856486363 34.69546147345739 23.292425236559772 17.157827641346536 8 24.122706988048595 38.35197584529551 20.905171680174828 16.620145486481057 9 24.246438401034553 16.79585837831859 23.061805280822878 35.895897939823975 10 14.763747245426126 31.454756657954526 32.99680554843779 20.784690548181555 11 31.363511370185943 26.98809048473234 22.090026706804913 19.558371438276804 12 20.28491429514596 29.261994723368197 32.131386600486145 18.3217043809997 13 26.57390848623346 24.325881029721298 27.2505372703095 21.84967321373574 14 19.710595229789906 23.841521621129544 29.448994280988757 26.998888868091797 15 23.43568408245914 30.178411904336507 25.003190484698436 21.382713528505917 16 20.130340271485515 32.06404709946103 27.529617954967943 20.275994674085513 17 18.84580895127218 31.04894902610717 29.967091661803014 20.13815036081764 18 18.98428001766233 29.90463534014019 31.619310471565438 19.49177417063204 19 19.560142251867482 29.2307168745427 32.10323469856557 19.10590617502425 20 21.126864814507588 28.24538342755778 31.199904712073067 19.42784704586157 21 21.144408976130354 29.258012700530212 30.34301981309938 19.25455851024006 22 19.516469903586657 28.896861886951697 31.51700420978835 20.06966399967329 23 19.153537646356174 29.278126504824797 31.536703687129165 20.03163216168986 24 19.418727124536108 28.947256475744986 31.302482758855387 20.331533640863512 25 20.121340617286997 28.326593605922906 30.206598948925826 21.34546682786427 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 962.0 1 962.0 2 1097.0 3 1232.0 4 1232.0 5 1232.0 6 2668.5 7 4105.0 8 4105.0 9 4105.0 10 5544.5 11 6984.0 12 6984.0 13 6984.0 14 9899.0 15 12814.0 16 12814.0 17 12814.0 18 19812.5 19 26811.0 20 26811.0 21 26811.0 22 38649.0 23 50487.0 24 50487.0 25 50487.0 26 65032.0 27 79577.0 28 79577.0 29 79577.0 30 93925.0 31 108273.0 32 108273.0 33 108273.0 34 119002.5 35 129732.0 36 129732.0 37 129732.0 38 134459.0 39 139186.0 40 139186.0 41 139186.0 42 137219.5 43 135253.0 44 135253.0 45 135253.0 46 143127.5 47 151002.0 48 151002.0 49 151002.0 50 137347.0 51 123692.0 52 123692.0 53 123692.0 54 105100.0 55 86508.0 56 86508.0 57 86508.0 58 72209.0 59 57910.0 60 57910.0 61 57910.0 62 46468.0 63 35026.0 64 35026.0 65 35026.0 66 25760.5 67 16495.0 68 16495.0 69 16495.0 70 11426.0 71 6357.0 72 6357.0 73 6357.0 74 4261.0 75 2165.0 76 2165.0 77 2165.0 78 1401.0 79 637.0 80 637.0 81 637.0 82 389.5 83 142.0 84 142.0 85 142.0 86 85.0 87 28.0 88 28.0 89 28.0 90 16.5 91 5.0 92 5.0 93 5.0 94 3.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09749988514411437 2 0.0 3 0.14939772976707225 4 0.013357314107876052 5 0.021780079054880695 6 0.01599474555592801 7 0.026629549781943974 8 0.02501305953958955 9 8.507843380812771E-5 10 0.39187126612023626 11 0.06627609993653148 12 0.0012761765071219156 13 0.0 14 6.806274704650217E-4 15 0.0013612549409300434 16 8.507843380812771E-5 17 0.0 18 2.5523530142438315E-4 19 5.104706028487663E-4 20 8.507843380812771E-5 21 1.7015686761625543E-4 22 0.002552353014243831 23 0.0 24 0.0 25 0.001446333374738171 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1175386.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.89522298618719 #Duplication Level Percentage of deduplicated Percentage of total 1 92.39910995718093 73.822474937542 2 6.0432445036482525 9.656527343580546 3 0.7718294075890273 1.8499644787986629 4 0.24123451596090797 0.770939417786467 5 0.1203990074098828 0.4809652772164095 6 0.08008600524645888 0.3839093548343273 7 0.05695746794090069 0.3185440721306808 8 0.039843209864323846 0.25466257092764855 9 0.02863540828661848 0.20590490973239056 >10 0.1865340955331003 2.8214117157913883 >50 0.017517118751952762 0.9834101246530839 >100 0.01055709938451142 1.6500298119638503 >500 0.0014929169695876063 0.7879945459126163 >1k 0.00202610160158318 2.9541488685235224 >5k 5.331846319955736E-4 3.0591125706063793 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9164 0.7796587674176824 No Hit CCCATGTACTCTGCGTTGATACCAC 7113 0.6051628996772124 No Hit GGTATCAACGCAGAGTACTTTTTTT 7049 0.5997178799134922 No Hit GTACATGGGAAGCAGTGGTATCAAC 6748 0.5741092713372458 No Hit TATCAACGCAGAGTACTTTTTTTTT 5832 0.4961774259690009 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4108 0.3495022060837886 No Hit GAGTACATGGGAAGCAGTGGTATCA 3372 0.28688447880100665 No Hit CATGTACTCTGCGTTGATACCACTG 2742 0.2332850655018862 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2176 0.1851306719664859 No Hit GCGTTGATACCACTGCTTCCCATGT 2042 0.1737301618361968 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1999 0.1700717891824473 No Hit GGTATCAACGCAGAGTACATGGGAA 1828 0.15552337700125746 No Hit GTATCAACGCAGAGTACATGGGAAG 1817 0.15458751422936803 No Hit GCTTCCCATGTACTCTGCGTTGATA 1695 0.14420794530477646 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1689 0.1436974747019277 No Hit TATCAACGCAGAGTACATGGGAAGC 1531 0.13025508216024353 No Hit ATTCCATTCCATTCCATTCCATTCC 1492 0.12693702324172654 No Hit ACTCTGCGTTGATACCACTGCTTCC 1470 0.12506529769794775 No Hit GTGGTATCAACGCAGAGTACATGGG 1406 0.11962027793422755 No Hit GAATGGAATGGAATGGAATGGAATG 1197 0.10183888526832886 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 8.507843380812771E-5 0.0 0.0 0.0 0.0 9 8.507843380812771E-5 0.0 0.0 0.0 0.0 10 8.507843380812771E-5 0.0 0.0 0.0 0.0 11 8.507843380812771E-5 0.0 0.0 0.0 0.0 12 8.507843380812771E-5 0.0 0.0 0.0 0.0 13 8.507843380812771E-5 0.0 0.0 8.507843380812771E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCCG 65 5.3449585E-5 13.181224 5 GCTCCGC 55 0.0030202686 12.116074 7 TCTATAC 160 4.5474735E-11 11.283047 3 AAGACTG 135 4.5693014E-9 11.282694 5 ACACGGT 85 5.2085416E-5 11.199733 6 GTCCTGT 85 5.2517615E-5 11.190653 1 AGCTCCG 60 0.0057839793 11.106401 6 CCAGGAC 150 2.1227606E-9 10.768382 3 TAGGACT 115 8.57488E-7 10.761482 4 ACTGTCC 80 3.6974513E-4 10.709744 8 GTATAGA 255 0.0 10.44461 1 TAGGACA 195 1.4551915E-11 10.252063 4 GCCCTAA 75 0.002619044 10.146192 1 TATACTG 370 0.0 10.034355 5 GTCCTGC 95 1.6221497E-4 10.012689 1 TCCTAAG 190 9.822543E-11 10.001593 2 TCTGTCG 115 1.00041725E-5 9.933677 8 GACCATG 125 2.4996643E-6 9.900563 7 TGGACAG 135 6.2535764E-7 9.872357 5 GACTGTG 165 9.857104E-9 9.80825 7 >>END_MODULE