FastQCFastQC Report
Wed 25 May 2016
SRR1294657_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294657_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497887
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC73210.48875515976839373No Hit
CCCATGTACTCTGCGTTGATACCAC69080.4611829864335561No Hit
GTATCAACGCAGAGTACTTTTTTTT39710.2651067804180155No Hit
GAGTACATGGGAAGCAGTGGTATCA35990.24027179620358546No Hit
GGTATCAACGCAGAGTACTTTTTTT27250.18192293544172558No Hit
CATGTACTCTGCGTTGATACCACTG26570.17738320714446415No Hit
GCGTTGATACCACTGCTTCCCATGT24160.1612938759732877No Hit
TATCAACGCAGAGTACTTTTTTTTT22990.15348287287358794No Hit
GTATCAACGCAGAGTACATGGGAAG21920.1463394768764266No Hit
ACGCAGAGTACATGGGAAGCAGTGG19090.12744619587458866No Hit
GGTATCAACGCAGAGTACATGGGAA16940.11309264317001216No Hit
TATCAACGCAGAGTACATGGGAAGC16890.11275883961874293No Hit
GCTTCCCATGTACTCTGCGTTGATA16180.10801882919071999No Hit
ACTCTGCGTTGATACCACTGCTTCC16060.10721770066767387No Hit
GTGGTATCAACGCAGAGTACATGGG16060.10721770066767387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG1657.2759576E-1211.5158611
CGCCCTA1001.9208346E-611.40413316
TCTATAC2150.011.0442383
CTTAGAC951.3630961E-510.9977363
GTATTAC1658.0035534E-1110.9400681
CTTATAC2100.010.854913
GTTCTAG1701.382432E-1010.61831
GTCCCGC1106.01988E-610.36704612
TGTCCCG1106.0656803E-610.36046411
GCCCTAG951.642709E-410.0006151
TAGACAG1901.0004442E-109.9972735
GTACTGG3650.09.891021
GTATAAC1252.5667396E-69.8806081
GTCTAGG1856.475602E-109.7573581
GGGCTAT1002.7529788E-49.5005851
GTACTAG1201.6984639E-59.5005851
TTAGACA1804.1964086E-99.4974114
TAGCCTG1401.0618332E-69.4974115
CTGTCGC800.00452669769.4970929
TTATACT2252.7284841E-119.2863564
Produced by FastQC (version 0.11.5)