##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294657_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1497887 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.826083008931917 31.0 30.0 33.0 25.0 34.0 2 29.906631141067383 31.0 30.0 34.0 25.0 34.0 3 29.854507716536695 31.0 30.0 34.0 25.0 34.0 4 33.458416422600635 35.0 33.0 37.0 27.0 37.0 5 33.81002171725905 35.0 35.0 37.0 28.0 37.0 6 33.93219381702358 36.0 35.0 37.0 28.0 37.0 7 33.81953378325601 36.0 35.0 37.0 28.0 37.0 8 33.66225489639739 36.0 35.0 37.0 27.0 37.0 9 34.7893639506852 38.0 34.0 39.0 27.0 39.0 10 34.91255615410241 38.0 34.0 39.0 27.0 39.0 11 35.162423467190784 38.0 35.0 39.0 27.0 39.0 12 34.92750053909273 38.0 35.0 39.0 27.0 39.0 13 34.77508516997611 38.0 34.0 39.0 27.0 39.0 14 35.66061258292515 39.0 34.0 41.0 26.0 41.0 15 35.64304450202185 39.0 34.0 41.0 26.0 41.0 16 35.74688744878619 39.0 34.0 41.0 27.0 41.0 17 35.72846082514903 39.0 34.0 41.0 27.0 41.0 18 35.6191788833203 39.0 34.0 41.0 26.0 41.0 19 35.894915304024934 39.0 34.0 41.0 27.0 41.0 20 36.027711035612164 39.0 35.0 41.0 27.0 41.0 21 36.05959394800809 39.0 35.0 41.0 27.0 41.0 22 36.06583340398842 39.0 35.0 41.0 27.0 41.0 23 36.04306933700606 39.0 35.0 41.0 27.0 41.0 24 36.0652232110967 39.0 35.0 41.0 27.0 41.0 25 34.80454733901823 38.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1326745597286632 1101 2 1.1009472090691155 1101 3 1.4403662927913956 1101 4 1.2905540875330317 1101 5 0.777478450433783 1101 6 0.8778690997843412 1101 7 0.8541035987469314 1101 8 1.0870545092509971 1101 9 1.5140256471509659 1101 10 1.3630369404625 1101 11 1.0759516823019908 1101 12 1.3140009698791602 1101 13 1.4610807270256032 1101 14 1.6388682105799148 1101 15 1.6521559124855756 1101 16 1.4738276481164831 1101 17 1.3473465502848967 1101 18 1.5847297887292413 1101 19 1.4574602965415764 1101 20 1.34277997826522 1101 21 1.2007144314889544 1101 22 1.0946547967154743 1101 23 1.1962817210291092 1101 24 1.3514795142540308 1101 25 1.2905097698067394 1102 1 0.8476044050172362 1102 2 0.83032819633992 1102 3 1.157473503500487 1102 4 1.026270505020797 1102 5 0.8147981621322558 1102 6 0.6342300058181465 1102 7 0.5590135208574978 1102 8 0.7157013331004336 1102 9 0.9088537295614216 1102 10 0.9590667310718572 1102 11 0.7312120814578194 1102 12 0.8946177097540087 1102 13 0.9006772627017909 1102 14 1.066788350368931 1102 15 1.1033450945702583 1102 16 1.036709346652735 1102 17 1.0576128082734684 1102 18 1.2598396615099077 1102 19 0.9646996644121302 1102 20 0.12755543647289613 1102 21 0.5431153402434106 1102 22 0.631482661764565 1102 23 0.6217272573634318 1102 24 0.7719988384780336 1102 25 0.7892970967194373 1103 1 0.7483802321839335 1103 2 0.7872142017379744 1103 3 0.9922182220184759 1103 4 0.8184973159443345 1103 5 0.7754679976921892 1103 6 0.6603447348343892 1103 7 0.5546289724662259 1103 8 0.6744217898656615 1103 9 0.8352510606126202 1103 10 0.7575113230112436 1103 11 0.8105416219016703 1103 12 1.0308772765787637 1103 13 0.9435091183909634 1103 14 0.7942982227933868 1103 15 1.1085647253936202 1103 16 1.0704368294690738 1103 17 1.042043859007535 1103 18 1.052268783705678 1103 19 0.9019310078394156 1103 20 0.04565739599162555 1103 21 0.5453255299553206 1103 22 -0.09689763662749584 1103 23 0.45565712457655394 1103 24 0.5566159424579809 1103 25 0.7866164723624749 1104 1 0.3180996706140675 1104 2 0.4283459765513733 1104 3 0.6388142993159462 1104 4 0.5150320419523524 1104 5 0.30255694221192186 1104 6 0.15661113025774398 1104 7 0.32118848198972927 1104 8 0.3630620936005613 1104 9 0.7366039798859063 1104 10 0.67829943242198 1104 11 0.33457846556621007 1104 12 0.56901473046711 1104 13 0.6099828171091275 1104 14 0.7825648960709586 1104 15 0.8906947479091443 1104 16 0.7166220163542576 1104 17 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-1.3399369884208667 2207 10 -0.26835202425979787 2207 11 0.18576473464378296 2207 12 0.5519954952300097 2207 13 0.592498456144483 2207 14 0.8290500329456449 2207 15 0.8795180708822201 2207 16 0.7866483057647287 2207 17 0.9464901034514384 2207 18 0.7516940361532605 2207 19 -0.4914792386048532 2207 20 0.1887952725563764 2207 21 -0.3966373612651424 2207 22 0.19469841400047017 2207 23 -0.5492035761803251 2207 24 -1.643988262822944 2207 25 -0.25631082299086927 2208 1 0.43770170625790783 2208 2 0.10743178262623587 2208 3 0.5785226439836606 2208 4 0.503331612087905 2208 5 0.37122312112514066 2208 6 0.30148001710240635 2208 7 0.32465997598498575 2208 8 0.5239053671598768 2208 9 -0.18428974869845405 2208 10 0.012678759243783588 2208 11 0.19040636935465471 2208 12 0.22438796683118767 2208 13 0.41674604695982254 2208 14 0.7762039128393852 2208 15 0.6481353528526199 2208 16 0.41752844406326517 2208 17 0.3774769755856866 2208 18 0.6602573967443135 2208 19 -1.2557301363869087 2208 20 -0.04327953221872605 2208 21 -0.31176118934643426 2208 22 0.07607236955840335 2208 23 0.20575019064287403 2208 24 0.5141778162135608 2208 25 0.5571297194308826 2209 1 0.2636637271496163 2209 2 -1.389790522298302 2209 3 -0.23649411150529076 2209 4 0.15599269689968054 2209 5 0.1429982546342501 2209 6 0.28044892875097105 2209 7 0.22849251151665584 2209 8 0.3195543432500827 2209 9 -2.057760720737349 2209 10 -0.420456860422604 2209 11 0.03289826728455836 2209 12 0.3696045200854101 2209 13 0.46298711648821467 2209 14 0.7212831802512554 2209 15 0.8721833113735471 2209 16 0.7134764717233324 2209 17 0.6234291383614874 2209 18 0.7758420414606846 2209 19 0.5418058859846226 2209 20 0.3430890311979553 2209 21 0.4817535156764592 2209 22 0.41032039049201074 2209 23 0.42817078815055964 2209 24 0.38737531926527424 2209 25 0.5883922970745701 2210 1 -0.012809207815841717 2210 2 0.1076828315584919 2210 3 -0.06542376657376536 2210 4 0.11998710281646652 2210 5 0.10866756098122465 2210 6 0.024635315447518735 2210 7 0.01856545180136493 2210 8 0.13747092403294658 2210 9 0.17791552280473866 2210 10 -1.4749854303630485 2210 11 -0.6226473885463406 2210 12 -2.6381810962056917 2210 13 -0.9718763892111468 2210 14 -0.5918682345597688 2210 15 -0.8637580083779568 2210 16 -0.28977603109698435 2210 17 -0.09862028195053085 2210 18 0.10305003098174836 2210 19 0.07460175292345639 2210 20 0.26533105286949166 2210 21 0.16825125166853638 2210 22 0.1032043354288561 2210 23 0.010091305182150734 2210 24 -0.027795861010929457 2210 25 -0.1244310970090865 2211 1 -0.3431048665754979 2211 2 -0.0503256393551581 2211 3 -1.457665210937261 2211 4 -0.5533983951001105 2211 5 -0.30459955818096063 2211 6 -0.4378767069659659 2211 7 -0.40692714129430385 2211 8 -0.14356611117472085 2211 9 -0.11554593069647012 2211 10 0.05859500558578645 2211 11 -0.18542736843237861 2211 12 -0.25597810984504576 2211 13 -0.34162614464678853 2211 14 -0.6632631912516374 2211 15 -0.2204110507860335 2211 16 0.05596731421417189 2211 17 -0.15073341406193208 2211 18 0.05747393613605567 2211 19 0.08673873091746742 2211 20 0.019688277362419626 2211 21 -0.23270738574095873 2211 22 -0.04298958455709112 2211 23 -0.4410705984821277 2211 24 -0.20773005844879577 2211 25 0.010699458512164028 2212 1 -0.3436879813341278 2212 2 -0.3401702590575688 2212 3 -2.265614137749761 2212 4 -1.286613562455038 2212 5 -0.6742654456191488 2212 6 -0.4316489766362608 2212 7 -0.28770331855521647 2212 8 -0.25821697525721277 2212 9 -0.836285602932719 2212 10 -0.20862996297504566 2212 11 -0.3217230392221637 2212 12 0.15020375277232034 2212 13 -0.43261693451965755 2212 14 -0.0029486925498929395 2212 15 0.026005705289072978 2212 16 -1.0514624338966811 2212 17 -1.1041673577120932 2212 18 -0.20377615936983062 2212 19 -0.044050201201059735 2212 20 -0.08028989627636207 2212 21 0.11415928704803946 2212 22 -0.3073893210479568 2212 23 -0.03777942213085339 2212 24 -0.16909914122147995 2212 25 0.11880440523170677 2213 1 -3.1243418533026954 2213 2 -1.5343446072083395 2213 3 -2.475063895582526 2213 4 -1.0819882499770657 2213 5 -0.8881978002038835 2213 6 -0.8735935831157775 2213 7 -0.49516519547643867 2213 8 -0.3297575746632617 2213 9 -0.3279318293429938 2213 10 -0.35972538534515053 2213 11 -0.5179603549406124 2213 12 -0.3146768900920449 2213 13 -0.6764377368946413 2213 14 -0.42285000212247326 2213 15 -0.36274473016245423 2213 16 -1.7404457146012433 2213 17 -1.024814298211794 2213 18 -0.487529015982922 2213 19 -0.3610463978320695 2213 20 -0.33525710843651524 2213 21 -0.2116288163651845 2213 22 -0.16002741664603803 2213 23 -0.05565655890809751 2213 24 -0.046029940950553794 2213 25 -0.027021925972981364 2214 1 -0.8336836686531583 2214 2 -0.7331377420757264 2214 3 -6.28826072145268 2214 4 -1.9423145260070847 2214 5 -1.0670718621292323 2214 6 -0.7543835537931756 2214 7 -0.5949697114879058 2214 8 -0.5175899388598708 2214 9 -0.40867458566723514 2214 10 -0.18288278308880024 2214 11 -1.0649904064622362 2214 12 -0.48314929868671186 2214 13 -4.927361315908275 2214 14 -3.8286449427317883 2214 15 -2.259212765217036 2214 16 -3.017109399308765 2214 17 -3.7779536385361645 2214 18 -1.3341527755707432 2214 19 -0.8342957393160901 2214 20 -0.6208169712664002 2214 21 -0.6756444385178284 2214 22 -0.596329455974768 2214 23 -0.23309141138395262 2214 24 -0.4047534139389768 2214 25 -0.2860796268824757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 594.0 3 78.0 4 86.0 5 172.0 6 26.0 7 43.0 8 114.0 9 271.0 10 680.0 11 1267.0 12 2433.0 13 4003.0 14 6124.0 15 8163.0 16 9985.0 17 11346.0 18 12259.0 19 12461.0 20 12455.0 21 12504.0 22 12688.0 23 13098.0 24 13669.0 25 14811.0 26 16207.0 27 18122.0 28 21172.0 29 25473.0 30 31064.0 31 38657.0 32 49943.0 33 65492.0 34 87264.0 35 118711.0 36 166292.0 37 249741.0 38 460419.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92037799401198 21.607209687767323 13.877178678357572 22.595233639863128 2 14.31364494096822 21.46425529783077 41.4018669739325 22.82023278726851 3 18.991978014857605 26.959855338404324 29.121963699665532 24.926202947072536 4 12.029158113900468 18.291553084372968 37.90541447712512 31.773874324601444 5 12.517575329280684 39.4422784439797 34.55765609731172 13.482490129427898 6 30.144051479597493 38.63060903538555 17.866939777903376 13.358399707113586 7 25.79730360768233 33.88561209281383 22.57214796821942 17.744936331284418 8 24.105450700000734 38.34696013900478 20.69459632968074 16.852992831313752 9 24.096282514221578 18.617024474812023 22.37760088719941 34.90909212376699 10 14.907939392319822 31.571777878731194 32.75032735629726 20.76995537265172 11 31.368897113101102 27.532725201829074 22.26516181397322 18.833215871096606 12 20.659766294080395 29.024987240501186 31.10324906274467 19.21199740267375 13 26.06911976613141 24.675447872916788 26.80042674604802 22.45500561490378 14 19.853721236608706 24.64893364010721 28.299222109097126 27.198123014186955 15 22.981707549268563 31.175530966090726 24.22786029567484 21.614901188965874 16 20.61160085615569 32.40364908305298 26.5885242002934 20.39622586049793 17 19.69046117503701 31.360664104880044 28.786963748403338 20.16191097167961 18 19.92030460755702 30.513507098706622 29.932372575959672 19.633815717776688 19 20.299412679207666 30.11403683999901 30.29141560278381 19.29513487800951 20 21.532729684988556 29.175000567840964 29.480766224552372 19.811503522618107 21 21.497541618790585 29.649680677658125 29.088862464259947 19.763915239291343 22 20.52362274951047 29.315004065228035 29.471667331399924 20.689705853861565 23 20.18386501333793 29.691373736604447 29.624366757323166 20.500394492734454 24 20.40817962789989 29.58393632900245 29.42765855946506 20.580225483632592 25 20.81366372485486 28.97682404580408 28.59180523914191 21.617706990199157 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2110.0 1 2110.0 2 2114.0 3 2118.0 4 2118.0 5 2118.0 6 2988.5 7 3859.0 8 3859.0 9 3859.0 10 5630.5 11 7402.0 12 7402.0 13 7402.0 14 11754.0 15 16106.0 16 16106.0 17 16106.0 18 24711.5 19 33317.0 20 33317.0 21 33317.0 22 47212.0 23 61107.0 24 61107.0 25 61107.0 26 79452.0 27 97797.0 28 97797.0 29 97797.0 30 116186.5 31 134576.0 32 134576.0 33 134576.0 34 148494.0 35 162412.0 36 162412.0 37 162412.0 38 169214.0 39 176016.0 40 176016.0 41 176016.0 42 175119.0 43 174222.0 44 174222.0 45 174222.0 46 181801.5 47 189381.0 48 189381.0 49 189381.0 50 172522.0 51 155663.0 52 155663.0 53 155663.0 54 133799.0 55 111935.0 56 111935.0 57 111935.0 58 94142.0 59 76349.0 60 76349.0 61 76349.0 62 62660.5 63 48972.0 64 48972.0 65 48972.0 66 37189.0 67 25406.0 68 25406.0 69 25406.0 70 18499.5 71 11593.0 72 11593.0 73 11593.0 74 8240.5 75 4888.0 76 4888.0 77 4888.0 78 3303.5 79 1719.0 80 1719.0 81 1719.0 82 1170.5 83 622.0 84 622.0 85 622.0 86 415.0 87 208.0 88 208.0 89 208.0 90 140.0 91 72.0 92 72.0 93 72.0 94 46.0 95 20.0 96 20.0 97 20.0 98 18.5 99 17.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10461403296777394 2 0.054209696726121526 3 0.05848238218236756 4 0.05394265388510615 5 0.050738139792921626 6 0.07083311357932875 7 0.06662718883333656 8 0.06409028184369048 9 0.06956466008450571 10 0.06989846363577493 11 0.06355619616165972 12 0.06429056397445201 13 0.06529197462825968 14 0.06802916374866728 15 0.06943113866399803 16 0.06429056397445201 17 0.06776212090765191 18 0.1463394768764266 19 0.07276917417669022 20 0.06602634244105196 21 0.06455760681546739 22 0.06983170292552109 23 0.06863001014095188 24 0.07964552733283618 25 0.07190128494339025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1497887.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.32573983473648 #Duplication Level Percentage of deduplicated Percentage of total 1 92.67031341508616 77.21822426028959 2 5.714409040820044 9.52314722089274 3 0.8422728079158712 2.105490145868125 4 0.25377490933965224 0.8458392828887878 5 0.12884064921713334 0.5367871208402699 6 0.08693919705231457 0.43465637490132314 7 0.04973704704555056 0.29010633695859156 8 0.037766239459808885 0.2517519875011515 9 0.032440795852075065 0.24328379836816247 >10 0.16040231335275382 2.389330027598489 >50 0.010954955026467718 0.6194355526856842 >100 0.008850189046457558 1.3344058307196327 >500 0.0017698950557322518 0.9507626588263028 >1k 0.001367646179429467 2.3029331157948816 >5k 1.6089955052111377E-4 0.9538462858661398 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 7321 0.48875515976839373 No Hit CCCATGTACTCTGCGTTGATACCAC 6908 0.4611829864335561 No Hit GTATCAACGCAGAGTACTTTTTTTT 3971 0.2651067804180155 No Hit GAGTACATGGGAAGCAGTGGTATCA 3599 0.24027179620358546 No Hit GGTATCAACGCAGAGTACTTTTTTT 2725 0.18192293544172558 No Hit CATGTACTCTGCGTTGATACCACTG 2657 0.17738320714446415 No Hit GCGTTGATACCACTGCTTCCCATGT 2416 0.1612938759732877 No Hit TATCAACGCAGAGTACTTTTTTTTT 2299 0.15348287287358794 No Hit GTATCAACGCAGAGTACATGGGAAG 2192 0.1463394768764266 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1909 0.12744619587458866 No Hit GGTATCAACGCAGAGTACATGGGAA 1694 0.11309264317001216 No Hit TATCAACGCAGAGTACATGGGAAGC 1689 0.11275883961874293 No Hit GCTTCCCATGTACTCTGCGTTGATA 1618 0.10801882919071999 No Hit ACTCTGCGTTGATACCACTGCTTCC 1606 0.10721770066767387 No Hit GTGGTATCAACGCAGAGTACATGGG 1606 0.10721770066767387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 6.676071025384425E-5 0.0 7 0.0 0.0 0.0 1.335214205076885E-4 0.0 8 0.0 0.0 0.0 1.335214205076885E-4 0.0 9 0.0 0.0 0.0 2.0028213076153274E-4 0.0 10 0.0 0.0 0.0 2.0028213076153274E-4 0.0 11 0.0 0.0 0.0 2.0028213076153274E-4 0.0 12 0.0 0.0 0.0 2.0028213076153274E-4 0.0 13 0.0 0.0 0.0 2.0028213076153274E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTAG 165 7.2759576E-12 11.515861 1 CGCCCTA 100 1.9208346E-6 11.404133 16 TCTATAC 215 0.0 11.044238 3 CTTAGAC 95 1.3630961E-5 10.997736 3 GTATTAC 165 8.0035534E-11 10.940068 1 CTTATAC 210 0.0 10.85491 3 GTTCTAG 170 1.382432E-10 10.6183 1 GTCCCGC 110 6.01988E-6 10.367046 12 TGTCCCG 110 6.0656803E-6 10.360464 11 GCCCTAG 95 1.642709E-4 10.000615 1 TAGACAG 190 1.0004442E-10 9.997273 5 GTACTGG 365 0.0 9.89102 1 GTATAAC 125 2.5667396E-6 9.880608 1 GTCTAGG 185 6.475602E-10 9.757358 1 GGGCTAT 100 2.7529788E-4 9.500585 1 GTACTAG 120 1.6984639E-5 9.500585 1 TTAGACA 180 4.1964086E-9 9.497411 4 TAGCCTG 140 1.0618332E-6 9.497411 5 CTGTCGC 80 0.0045266976 9.497092 9 TTATACT 225 2.7284841E-11 9.286356 4 >>END_MODULE