FastQCFastQC Report
Wed 25 May 2016
SRR1294657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294657_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497887
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98890.6601966637002658No Hit
CCCATGTACTCTGCGTTGATACCAC79480.5306141250975541No Hit
GGTATCAACGCAGAGTACTTTTTTT74440.4969667271296166No Hit
GTACATGGGAAGCAGTGGTATCAAC70590.4712638536818865No Hit
TATCAACGCAGAGTACTTTTTTTTT59440.3968256617488502No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39370.2628369162693848No Hit
GAGTACATGGGAAGCAGTGGTATCA35790.23893658199850856No Hit
CATGTACTCTGCGTTGATACCACTG29410.19634324885655594No Hit
ACGCAGAGTACATGGGAAGCAGTGG25190.16817022912943366No Hit
GCGTTGATACCACTGCTTCCCATGT24920.16636768995257986No Hit
GTATCAACGCAGAGTACATGGGAAG23520.15702119051704166No Hit
GGTATCAACGCAGAGTACATGGGAA19800.1321862063026116No Hit
ACTCTGCGTTGATACCACTGCTTCC18900.12617774237976562No Hit
TATCAACGCAGAGTACATGGGAAGC17650.1178326535980351No Hit
GCTTCCCATGTACTCTGCGTTGATA17440.11643067868270437No Hit
GTGGTATCAACGCAGAGTACATGGG16470.10995488978808148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG350.002201735716.2505218
CGTCGGT400.005355289714.21920519
GCGTACT500.001524529213.27081512
ACACCGT550.003008684412.1226496
CTAGGAC1052.7249916E-711.760043
GCTCTAG907.3817646E-611.62211
TAGGACT1655.456968E-1211.5453814
TAGACAG2400.011.5093015
CCTAGAC1451.2205419E-911.1361673
CGGGTTC600.005970854311.059381518
CGTACTT600.00597233811.05901213
GTTGGAC951.3623778E-510.9982583
TCTGCGA700.001459076510.8856448
GACAGTG2100.010.8856447
TTACACC1553.5051926E-910.4467074
GTGTAGG1951.4551915E-1110.2404531
TCTGCAC2250.010.1317283
TCTAGTA1502.4489054E-810.1317282
CTAGCAC1901.0004442E-109.9984163
CTGGGCG1159.9496865E-69.9387339
Produced by FastQC (version 0.11.5)