##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294657_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1497887 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.212538729557036 31.0 31.0 34.0 28.0 34.0 2 31.3673561490286 31.0 31.0 34.0 28.0 34.0 3 31.513080759763586 31.0 31.0 34.0 28.0 34.0 4 35.090057527704026 37.0 35.0 37.0 32.0 37.0 5 35.20740883658113 37.0 35.0 37.0 32.0 37.0 6 34.81018327817786 37.0 35.0 37.0 32.0 37.0 7 34.65215400093599 37.0 35.0 37.0 31.0 37.0 8 34.70195815839246 37.0 35.0 37.0 31.0 37.0 9 36.1206105667517 39.0 35.0 39.0 30.0 39.0 10 35.9182608567936 39.0 35.0 39.0 30.0 39.0 11 36.053771078859754 39.0 35.0 39.0 30.0 39.0 12 35.990525987607874 39.0 35.0 39.0 30.0 39.0 13 35.8459623456242 39.0 35.0 39.0 30.0 39.0 14 36.93165439048473 40.0 36.0 41.0 30.0 41.0 15 36.97743955318392 40.0 36.0 41.0 30.0 41.0 16 37.00924101751334 40.0 36.0 41.0 30.0 41.0 17 37.0737445481535 40.0 36.0 41.0 30.0 41.0 18 37.07571999756991 40.0 36.0 41.0 30.0 41.0 19 37.09700197678463 40.0 37.0 41.0 30.0 41.0 20 37.16243548411863 40.0 37.0 41.0 30.0 41.0 21 37.00184459842431 40.0 36.0 41.0 30.0 41.0 22 37.01449441780321 40.0 36.0 41.0 30.0 41.0 23 37.01796130148669 40.0 36.0 41.0 30.0 41.0 24 36.94978593178257 40.0 36.0 41.0 30.0 41.0 25 35.843052246264236 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6048296048377217 1101 2 0.6077725462137984 1101 3 0.5023680697505775 1101 4 0.39796713000330897 1101 5 0.47774413511775293 1101 6 0.5649240799866462 1101 7 0.7033471087700534 1101 8 0.62860520964729 1101 9 0.8286485007639115 1101 10 0.7373780136934158 1101 11 0.7509420116314729 1101 12 0.9189130242522765 1101 13 0.8823287695329327 1101 14 1.1077824224176709 1101 15 0.9854262710136013 1101 16 1.046800429306714 1101 17 1.0935524081085148 1101 18 0.9877180920131039 1101 19 1.2067325473659594 1101 20 1.1238215392848332 1101 21 1.2812755615418467 1101 22 1.1782251996292104 1101 23 1.155254663573821 1101 24 1.4034764227047987 1101 25 1.1932870602162353 1102 1 0.37184327512473914 1102 2 0.4223439388925918 1102 3 0.3307422721033575 1102 4 0.296396318971901 1102 5 0.30588584794950435 1102 6 0.4699151374303199 1102 7 0.49962783314978765 1102 8 0.5420489131751012 1102 9 0.6447486845044637 1102 10 0.7147467745085407 1102 11 0.5924001336244515 1102 12 0.7139297608352351 1102 13 0.7229546008251759 1102 14 1.0727462152911755 1102 15 1.0380711691091236 1102 16 0.9553997337987639 1102 17 0.9834802988391687 1102 18 0.9272509684034702 1102 19 1.0025580957495563 1102 20 0.969816535132459 1102 21 1.2616159765471409 1102 22 1.2333143238396076 1102 23 1.0317356799148953 1102 24 1.137875480256909 1102 25 0.958969452662906 1103 1 0.3424000381833814 1103 2 0.31420490766133824 1103 3 0.30237713486928186 1103 4 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0.2771752698451344 2207 4 0.25890330128430605 2207 5 0.24986735551684092 2207 6 0.42884024275338817 2207 7 0.39888486177906657 2207 8 0.3456681451010297 2207 9 0.5264309226842698 2207 10 0.6868645953639074 2207 11 0.5680579781727317 2207 12 0.435111563439321 2207 13 0.5245645267112238 2207 14 0.536872954956479 2207 15 0.53137765604545 2207 16 0.6761225413874499 2207 17 0.7291294689729284 2207 18 0.6645819987620669 2207 19 0.7949696873272387 2207 20 0.7490883944367468 2207 21 0.9911704093677329 2207 22 0.8858785164779306 2207 23 0.7365768425971524 2207 24 1.0210302356609091 2207 25 0.9588561821071977 2208 1 0.1527729697257314 2208 2 0.18864522470235556 2208 3 0.1605977625653523 2208 4 0.08116770218371983 2208 5 0.10199544800101279 2208 6 0.2222370025200746 2208 7 0.10779060233663529 2208 8 0.1182825003068757 2208 9 0.3486091840316661 2208 10 0.4071298655797193 2208 11 0.037366059745892244 2208 12 -0.07894750057028688 2208 13 0.10940356951267916 2208 14 -0.3914470564495858 2208 15 0.12286429599895143 2208 16 0.2524763432605397 2208 17 0.12644864769666242 2208 18 0.31723861185389524 2208 19 0.5577532423956626 2208 20 0.45373171233426746 2208 21 0.7104119060572955 2208 22 0.5836009669204572 2208 23 0.7519294850516687 2208 24 0.6918415019222266 2208 25 0.7348864160998403 2209 1 0.06399710733355946 2209 2 -0.00469569518997659 2209 3 0.10037349594190914 2209 4 -0.5724186561246114 2209 5 -0.11835571705405812 2209 6 0.23569079453147168 2209 7 0.27586574455705204 2209 8 0.22765090824321277 2209 9 0.3338383300916732 2209 10 0.3038475761150039 2209 11 0.37106912756068766 2209 12 0.3286082931679388 2209 13 0.5446897801698043 2209 14 0.42544748266144694 2209 15 -0.2778833329256898 2209 16 -0.12071476414551086 2209 17 0.21884069636531933 2209 18 -0.2896780583965395 2209 19 0.21862578489638196 2209 20 0.41584534090383585 2209 21 0.6833274493508554 2209 22 0.6726367351202072 2209 23 0.6564955349796122 2209 24 0.5620745129370661 2209 25 0.6129448303987459 2210 1 -0.2189701947624485 2210 2 -0.20731498502510348 2210 3 -0.1840865220992285 2210 4 -0.20134919773558835 2210 5 -0.16863464549013685 2210 6 -0.5709773349303262 2210 7 -0.28551491241527316 2210 8 -0.3297411939928665 2210 9 -0.17053542736359617 2210 10 -0.059436709254164555 2210 11 -0.07376111063111779 2210 12 -0.030317749239827663 2210 13 -0.04022126637146073 2210 14 -0.033211045545016304 2210 15 -0.12035492477924947 2210 16 0.03584110687142328 2210 17 -1.0938359749335334 2210 18 -1.6436642859587067 2210 19 -0.42212613613041583 2210 20 -0.23461266911852618 2210 21 -0.5305819593031273 2210 22 -0.39716363767092133 2210 23 -0.20035843595509562 2210 24 0.12336053149683579 2210 25 0.051243466674733895 2211 1 -0.28832014063586087 2211 2 -0.22200736902339102 2211 3 -0.16076681302448748 2211 4 -0.13026226179430012 2211 5 -0.16089433785354856 2211 6 -0.11223084904234071 2211 7 -0.2655580490406564 2211 8 -0.19683812928930422 2211 9 -0.27837699081929657 2211 10 -0.053836768525862055 2211 11 -0.4117147552231941 2211 12 -0.14144532471176774 2211 13 -0.22051699373261613 2211 14 -0.283646322084536 2211 15 -0.2406003260774625 2211 16 -0.32973424707803645 2211 17 -0.3836862452920613 2211 18 -0.404998969369629 2211 19 -0.18292435578419486 2211 20 -0.4226692845673057 2211 21 -0.036266965895734415 2211 22 -0.15295120351121483 2211 23 -0.753951988511389 2211 24 -0.26884867016002545 2211 25 -0.7641162727493196 2212 1 -0.11335099494751333 2212 2 -0.27347900582875795 2212 3 -0.11712466778934072 2212 4 -0.03496070336224477 2212 5 0.012729388122828311 2212 6 -0.0596340471393475 2212 7 -0.10362834983379798 2212 8 -0.2738375247712739 2212 9 0.005962383463476328 2212 10 -0.41665808827657713 2212 11 -0.14773474687591914 2212 12 -0.3031621111247702 2212 13 -0.23252734097086147 2212 14 -0.09608383883695382 2212 15 -0.05899069042339278 2212 16 -0.2100633199156121 2212 17 -0.4430413796013326 2212 18 -0.30281874733804415 2212 19 -0.1406900979538932 2212 20 -0.45960088144810385 2212 21 0.13418720907403525 2212 22 -0.5472169181801974 2212 23 -0.0597374425980135 2212 24 -0.22487079218506523 2212 25 -0.19938331715886903 2213 1 -0.20495748161733474 2213 2 -0.1695855073115915 2213 3 -0.11073719408243932 2213 4 -0.10546886124650001 2213 5 -0.058395936103394774 2213 6 -0.252230937019398 2213 7 -0.2713447398716866 2213 8 -0.18755606116378232 2213 9 -0.374943539596714 2213 10 -0.19153552335726687 2213 11 -0.06687842187226067 2213 12 -0.04571333605885286 2213 13 0.14947295733259125 2213 14 -0.5639998725452386 2213 15 -0.20771743526871944 2213 16 -0.25938095077327006 2213 17 -0.21902276730489234 2213 18 -0.1389944766746538 2213 19 -0.17859417398450006 2213 20 -0.30208695466117064 2213 21 0.07621047776473944 2213 22 -0.32494585841632073 2213 23 -0.38855761669689315 2213 24 -0.6080686460347664 2213 25 -0.47748360432701986 2214 1 -0.4358356685861722 2214 2 -0.27910209860095847 2214 3 -0.2886317652601491 2214 4 -0.23432677089313358 2214 5 -0.3026390983054057 2214 6 -0.4043833316982628 2214 7 -0.42697608688889943 2214 8 -0.394905762139544 2214 9 -0.4777113775106656 2214 10 -0.3785934064976004 2214 11 -0.3430285469488865 2214 12 -0.46793183083214274 2214 13 -0.5945589827631679 2214 14 -0.5944096434313835 2214 15 -0.11175012085426062 2214 16 -0.467824036769656 2214 17 -0.5247998989915246 2214 18 -0.489449225444595 2214 19 -0.6656619969886535 2214 20 -0.6274021311765878 2214 21 -1.00264577523793 2214 22 -0.8997184840313537 2214 23 -1.3259571826561753 2214 24 -0.6714539151565759 2214 25 -1.2132281829596252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 11.0 10 42.0 11 84.0 12 252.0 13 644.0 14 1378.0 15 2457.0 16 3655.0 17 4956.0 18 5806.0 19 6439.0 20 7065.0 21 7592.0 22 8262.0 23 8879.0 24 9754.0 25 11159.0 26 12645.0 27 14159.0 28 16753.0 29 20788.0 30 26119.0 31 33870.0 32 44822.0 33 60567.0 34 83069.0 35 114825.0 36 164004.0 37 258319.0 38 569510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.60329244781835 22.045500867049125 13.036743503814014 24.314463181318516 2 14.158144105663512 21.384657187090884 40.13994380083411 24.3172549064115 3 18.295871374107296 26.44843512784842 29.933490343097308 25.322203154946976 4 11.919369969386494 18.348406395117863 37.905996174146445 31.826227461349198 5 12.650916181419946 38.65076927186281 34.89228858379187 13.80602596292537 6 30.466680688119492 38.98965417088145 17.15985457750358 13.383810563495482 7 25.727157935785606 34.07811418043947 22.52171340772461 17.673014476050312 8 24.190209734176456 38.39717950601291 20.357970366116678 17.05464039369395 9 23.9884557071547 17.737500191937556 22.581002655080535 35.69304144582721 10 14.800680672871541 31.27281671629627 32.76679849844476 21.159704112387427 11 31.635471843824803 26.72252941612671 22.23366822924704 19.408330510801445 12 20.82854039388115 28.549072912542734 31.360678513321975 19.261708180254136 13 26.557277017558732 23.885446632489636 27.1376278717954 22.41964847815623 14 19.951024048687543 23.992474687524616 28.728975256996897 27.327526006790944 15 23.220352674836953 30.407038809186666 24.65085351369648 21.721755002279906 16 20.399282722450174 31.983208334946717 27.073288621430468 20.54422032117264 17 19.334502535905578 31.069032577223783 29.274304403469685 20.32216048340095 18 19.620343165235642 29.89740826862517 30.740199976099536 19.742048590039648 19 20.041712258851003 29.438520149464477 31.358832911292687 19.160934680391833 20 21.309794349896254 28.390516221549866 30.641958923387925 19.657730505165954 21 21.160893288585676 29.068664333147957 30.11821366316817 19.652228715098197 22 20.147144731263918 28.753242157900182 30.580801084224333 20.518812026611567 23 19.840281143641487 29.00729162911364 30.74441110848518 20.40801611875969 24 19.999773013585138 29.04424699593494 30.52626800286003 20.429711987619896 25 20.488551636472398 28.340975367458555 29.648243889933347 21.522229106135704 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1220.0 1 1220.0 2 1187.5 3 1155.0 4 1155.0 5 1155.0 6 2336.5 7 3518.0 8 3518.0 9 3518.0 10 5034.5 11 6551.0 12 6551.0 13 6551.0 14 10093.0 15 13635.0 16 13635.0 17 13635.0 18 22256.0 19 30877.0 20 30877.0 21 30877.0 22 45890.5 23 60904.0 24 60904.0 25 60904.0 26 80060.0 27 99216.0 28 99216.0 29 99216.0 30 118511.0 31 137806.0 32 137806.0 33 137806.0 34 151708.0 35 165610.0 36 165610.0 37 165610.0 38 170481.5 39 175353.0 40 175353.0 41 175353.0 42 173810.5 43 172268.0 44 172268.0 45 172268.0 46 181656.0 47 191044.0 48 191044.0 49 191044.0 50 174766.5 51 158489.0 52 158489.0 53 158489.0 54 135646.5 55 112804.0 56 112804.0 57 112804.0 58 94481.0 59 76158.0 60 76158.0 61 76158.0 62 62463.0 63 48768.0 64 48768.0 65 48768.0 66 36988.5 67 25209.0 68 25209.0 69 25209.0 70 18119.5 71 11030.0 72 11030.0 73 11030.0 74 7682.5 75 4335.0 76 4335.0 77 4335.0 78 2833.0 79 1331.0 80 1331.0 81 1331.0 82 878.5 83 426.0 84 426.0 85 426.0 86 276.0 87 126.0 88 126.0 89 126.0 90 81.0 91 36.0 92 36.0 93 36.0 94 25.5 95 15.0 96 15.0 97 15.0 98 9.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09560133708350496 2 0.0 3 0.1344560704512423 4 0.012150449266199653 5 0.02450118066316084 6 0.012150449266199653 7 0.020695820178691718 8 0.018492716740314857 9 2.6704284101537696E-4 10 0.38948198362092734 11 0.06702775309485962 12 0.0016022570460922621 13 0.0 14 6.008463922845982E-4 15 0.0013352142050768849 16 6.676071025384424E-5 17 0.0 18 5.340856820307539E-4 19 4.0056426152306553E-4 20 2.0028213076153276E-4 21 4.0056426152306553E-4 22 0.0028039498306614585 23 3.338035512692212E-4 24 0.0 25 0.002069582017869172 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1497887.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.87538645394515 #Duplication Level Percentage of deduplicated Percentage of total 1 91.96368093039378 74.37598234972903 2 6.318859081070979 10.220803402592724 3 0.8838622023559346 2.1444809156271387 4 0.2812803026464334 0.909946127136518 5 0.1343801748160592 0.543402428499875 6 0.08549205276314124 0.41485216835760713 7 0.05629393852032364 0.3186955822992063 8 0.04058919804476287 0.26261336621807296 9 0.034367824050638165 0.2501559946508897 >10 0.17611974442056333 2.5103057232650565 >50 0.012333106719453264 0.6960074913120036 >100 0.008852975962980274 1.3177900257053332 >500 0.0019028674341407654 1.0497655814982523 >1k 0.0015719339673336756 2.423580797211307 >5k 4.1366683350886206E-4 2.561618045897067 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9889 0.6601966637002658 No Hit CCCATGTACTCTGCGTTGATACCAC 7948 0.5306141250975541 No Hit GGTATCAACGCAGAGTACTTTTTTT 7444 0.4969667271296166 No Hit GTACATGGGAAGCAGTGGTATCAAC 7059 0.4712638536818865 No Hit TATCAACGCAGAGTACTTTTTTTTT 5944 0.3968256617488502 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3937 0.2628369162693848 No Hit GAGTACATGGGAAGCAGTGGTATCA 3579 0.23893658199850856 No Hit CATGTACTCTGCGTTGATACCACTG 2941 0.19634324885655594 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2519 0.16817022912943366 No Hit GCGTTGATACCACTGCTTCCCATGT 2492 0.16636768995257986 No Hit GTATCAACGCAGAGTACATGGGAAG 2352 0.15702119051704166 No Hit GGTATCAACGCAGAGTACATGGGAA 1980 0.1321862063026116 No Hit ACTCTGCGTTGATACCACTGCTTCC 1890 0.12617774237976562 No Hit TATCAACGCAGAGTACATGGGAAGC 1765 0.1178326535980351 No Hit GCTTCCCATGTACTCTGCGTTGATA 1744 0.11643067868270437 No Hit GTGGTATCAACGCAGAGTACATGGG 1647 0.10995488978808148 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.676071025384425E-5 0.0 4 0.0 0.0 0.0 6.676071025384425E-5 0.0 5 0.0 0.0 0.0 6.676071025384425E-5 0.0 6 0.0 0.0 0.0 6.676071025384425E-5 0.0 7 0.0 0.0 0.0 1.335214205076885E-4 0.0 8 0.0 0.0 0.0 1.335214205076885E-4 0.0 9 0.0 0.0 0.0 1.335214205076885E-4 0.0 10 0.0 0.0 0.0 2.0028213076153274E-4 0.0 11 0.0 0.0 0.0 2.0028213076153274E-4 0.0 12 0.0 0.0 0.0 2.0028213076153274E-4 0.0 13 0.0 0.0 0.0 3.338035512692212E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCGG 35 0.0022017357 16.25052 18 CGTCGGT 40 0.0053552897 14.219205 19 GCGTACT 50 0.0015245292 13.270815 12 ACACCGT 55 0.0030086844 12.122649 6 CTAGGAC 105 2.7249916E-7 11.76004 3 GCTCTAG 90 7.3817646E-6 11.6221 1 TAGGACT 165 5.456968E-12 11.545381 4 TAGACAG 240 0.0 11.509301 5 CCTAGAC 145 1.2205419E-9 11.136167 3 CGGGTTC 60 0.0059708543 11.0593815 18 CGTACTT 60 0.005972338 11.059012 13 GTTGGAC 95 1.3623778E-5 10.998258 3 TCTGCGA 70 0.0014590765 10.885644 8 GACAGTG 210 0.0 10.885644 7 TTACACC 155 3.5051926E-9 10.446707 4 GTGTAGG 195 1.4551915E-11 10.240453 1 TCTGCAC 225 0.0 10.131728 3 TCTAGTA 150 2.4489054E-8 10.131728 2 CTAGCAC 190 1.0004442E-10 9.998416 3 CTGGGCG 115 9.9496865E-6 9.938733 9 >>END_MODULE