FastQCFastQC Report
Wed 25 May 2016
SRR1294656_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294656_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230071
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT180560.5589970003755335No Hit
GGTATCAACGCAGAGTACTTTTTTT110900.34333610623419736No Hit
TATCAACGCAGAGTACTTTTTTTTT104700.3241414817197517No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42710.1322261956470926No Hit
GTACTGGTTCACTATCGGTCAGTCA42350.13111166906238286No Hit
GTACATGGGAAGCAGTGGTATCAAC38640.11962585342551295No Hit
CCCATGTACTCTGCGTTGATACCAC35960.1113288221837848No Hit
GGTATCAACGCAGAGTACATGGGGG32550.1007717787008397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA701.0859881E-412.22103515
GGTATCA43850.011.1786091
CCGAATT951.3530318E-511.00607814
AATCGCC2200.010.80275918
CTTGCAC2250.010.5517353
TACACTG4700.010.1025685
ATCGCCG2300.09.91052919
GTACTAG1752.579327E-99.77107051
GTATCAA86400.09.697561
GTGTAGG3050.09.6553841
GTCTACG800.00451903989.4996521
GTCCTAT1804.1909516E-99.4996511
GCAACCG1201.7089484E-59.4965619
CTAGTAC1502.6648377E-79.496563
TAGCACG800.00453176449.4964144
TAGACAG4200.09.4964135
ACGGTAC2950.09.3356033
CGCCCTA4900.09.1172816
AGGACCG1151.1158537E-49.0835265
ATCCCGT1151.1158537E-49.0835266
Produced by FastQC (version 0.11.5)