##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294656_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3230071 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.040611491202515 31.0 30.0 33.0 25.0 34.0 2 30.109302241343922 31.0 30.0 34.0 25.0 34.0 3 30.10942174336106 31.0 30.0 34.0 25.0 34.0 4 33.62930876751626 35.0 35.0 37.0 28.0 37.0 5 34.17678187259661 37.0 35.0 37.0 30.0 37.0 6 34.25294490430706 37.0 35.0 37.0 30.0 37.0 7 34.13919291557368 37.0 35.0 37.0 30.0 37.0 8 33.934494628755836 37.0 35.0 37.0 28.0 37.0 9 35.015363439379506 39.0 35.0 39.0 27.0 39.0 10 35.19527929881418 39.0 35.0 39.0 27.0 39.0 11 35.42713023955201 39.0 35.0 39.0 28.0 39.0 12 35.22583342595256 39.0 35.0 39.0 27.0 39.0 13 35.05310502462639 39.0 35.0 39.0 27.0 39.0 14 35.97541663944848 39.0 36.0 41.0 27.0 41.0 15 35.981833835850665 39.0 35.0 41.0 27.0 41.0 16 36.129355051328595 39.0 36.0 41.0 27.0 41.0 17 36.15401611915032 39.0 36.0 41.0 27.0 41.0 18 36.05702537188811 39.0 36.0 41.0 27.0 41.0 19 36.37622299943252 40.0 36.0 41.0 27.0 41.0 20 36.483751595553166 40.0 36.0 41.0 27.0 41.0 21 36.556772281476164 40.0 36.0 41.0 27.0 41.0 22 36.58454937987431 40.0 36.0 41.0 28.0 41.0 23 36.57812568206705 39.0 36.0 41.0 28.0 41.0 24 36.59253682039807 39.0 36.0 41.0 28.0 41.0 25 35.588598516874704 39.0 34.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0208079387532578 1101 2 1.0176914567789765 1101 3 1.3451689961272457 1101 4 1.1786791816906472 1101 5 0.8360992162626033 1101 6 0.8132296657481248 1101 7 0.8612410568647206 1101 8 0.9974443812473197 1101 9 1.242396392337234 1101 10 1.1855267440657258 1101 11 0.9740002911679397 1101 12 1.1244940270668167 1101 13 1.2641300216837479 1101 14 1.233128873398229 1101 15 1.379173275919804 1101 16 1.3052629373301627 1101 17 1.3347053724526745 1101 18 1.3449452201751981 1101 19 1.235128130347249 1101 20 1.0901001319324664 1101 21 1.043199812160168 1101 22 0.9069501226664229 1101 23 0.9636074815691131 1101 24 1.0762498132986806 1101 25 1.046648525608866 1102 1 0.8469827483072514 1102 2 0.8581853789527827 1102 3 1.1167464207511841 1102 4 0.9422729628761317 1102 5 0.7635789741395058 1102 6 0.6717449613046753 1102 7 0.6031736986154641 1102 8 0.7339299878244319 1102 9 1.0612747767355941 1102 10 0.8817751773070412 1102 11 0.6795249577377405 1102 12 0.977222382644797 1102 13 0.9268514316308512 1102 14 1.2196550201059182 1102 15 1.2748385316387711 1102 16 1.0778311122898003 1102 17 1.1431105119001899 1102 18 1.3419952909887058 1102 19 0.9590947402247352 1102 20 0.2347721305018311 1102 21 0.5806400517924146 1102 22 0.6742636324773272 1102 23 0.7562619420213323 1102 24 0.8039585694240046 1102 25 0.8913221847428474 1103 1 0.7316839241980837 1103 2 0.6841378643010181 1103 3 0.9514912362946433 1103 4 0.8067634471668512 1103 5 0.5738219803552695 1103 6 0.4479991441076052 1103 7 0.3450251738163175 1103 8 0.5902998696537907 1103 9 0.7253180138902877 1103 10 0.6536338683929372 1103 11 0.6124894009476591 1103 12 0.8108075450098653 1103 13 0.764016193319172 1103 14 0.7032661152752553 1103 15 0.8579912841966646 1103 16 0.837034870365585 1103 17 0.8747094005161529 1103 18 0.9111005595030335 1103 19 0.7813333174327894 1103 20 -0.14961330641886406 1103 21 0.27992368198710693 1103 22 -0.20786939232527146 1103 23 0.2839121575413799 1103 24 0.5101846615440024 1103 25 0.6047439604933373 1104 1 0.46112749129484953 1104 2 0.4076999754337862 1104 3 0.600843523873742 1104 4 0.543733021986931 1104 5 0.3903093100846249 1104 6 0.3125959774349738 1104 7 0.3540178770239564 1104 8 0.46942867445874015 1104 9 0.777063935770542 1104 10 0.7171335972328308 1104 11 0.45334896299868177 1104 12 0.5455439274468219 1104 13 0.4750892049996054 1104 14 0.8933569925831506 1104 15 0.7941837543878378 1104 16 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0.3071701550072845 2207 8 0.3828249980503742 2207 9 -1.1945931131218686 2207 10 -0.26508468060257684 2207 11 0.27011114112638523 2207 12 0.49199514530956634 2207 13 0.5767368526592591 2207 14 0.8351491700379157 2207 15 0.8801250136733003 2207 16 0.8200644851888157 2207 17 0.8026667995553538 2207 18 0.7800040877191705 2207 19 -0.4204140686176743 2207 20 0.21134791146254628 2207 21 -0.30527694333858335 2207 22 0.2257447153541534 2207 23 -0.4870527158509148 2207 24 -1.5472856730778375 2207 25 -0.13946983244918698 2208 1 0.5556726623640813 2208 2 0.21973336652716924 2208 3 0.6804426304802682 2208 4 0.6283164848685416 2208 5 0.40688910319910576 2208 6 0.2778187725881125 2208 7 0.4351104953208349 2208 8 0.563073136388148 2208 9 -0.18844466331673715 2208 10 0.057855988273161074 2208 11 0.30636415299655084 2208 12 0.35256248031698334 2208 13 0.5536976872048314 2208 14 0.8679170440314934 2208 15 0.7255820677113931 2208 16 0.49617645197652394 2208 17 0.5039659957372535 2208 18 0.7855272001774196 2208 19 -1.316333085905569 2208 20 -0.11821988689565188 2208 21 -0.3095267481534165 2208 22 0.14934558400772602 2208 23 0.41356297486190385 2208 24 0.49328691652203105 2208 25 0.5117910354811812 2209 1 0.20227668038485191 2209 2 -1.5363072377024736 2209 3 -0.18673474295487935 2209 4 0.10955420465391086 2209 5 0.1531239644600717 2209 6 0.20279374022451435 2209 7 0.17834506206716583 2209 8 0.3595487526078571 2209 9 -1.9325427117276206 2209 10 -0.24485089825021333 2209 11 -0.04328018969387415 2209 12 0.3287933937796268 2209 13 0.36754890768742143 2209 14 0.5286829343913837 2209 15 0.7344012637248554 2209 16 0.536459846792269 2209 17 0.4046515469403431 2209 18 0.645032307456006 2209 19 0.40094439052572284 2209 20 0.42055268534124934 2209 21 0.4563883904487227 2209 22 0.3909788410209458 2209 23 0.45288867369916375 2209 24 0.464118464020558 2209 25 0.49537687736361136 2210 1 0.09867724935428512 2210 2 0.1522730708633162 2210 3 0.03991615143105065 2210 4 0.1423811444607921 2210 5 0.09952722531705405 2210 6 -0.00523382067881073 2210 7 0.015680586479568603 2210 8 0.0702045517638652 2210 9 0.17070652234231432 2210 10 -1.6878440155717342 2210 11 -0.6318208998389707 2210 12 -2.6026716521862383 2210 13 -0.9506402520059183 2210 14 -0.6245141475018698 2210 15 -0.8357334765683717 2210 16 -0.296419723190013 2210 17 -0.0606379466980016 2210 18 0.20578217172952407 2210 19 0.10273054597191589 2210 20 0.1717870541539952 2210 21 0.12096460935804743 2210 22 0.08927963102704695 2210 23 0.07213300299902414 2210 24 0.02632194757462969 2210 25 0.011552102639377893 2211 1 -0.3807957306102132 2211 2 -0.1828679516405991 2211 3 -1.5939164073408918 2211 4 -0.7314700371985339 2211 5 -0.5843772227643029 2211 6 -0.35610577831693035 2211 7 -0.5202697063067987 2211 8 -0.1433607115172535 2211 9 0.028257661071549478 2211 10 -0.052788288352275003 2211 11 -0.2675502083277408 2211 12 -0.29520390793494045 2211 13 -0.3299447417763375 2211 14 -0.8379057318124339 2211 15 -0.3982679716263746 2211 16 -0.3169415631733372 2211 17 -0.24442203217745373 2211 18 -0.17359054205444835 2211 19 -0.23191070509771095 2211 20 -0.13686850332550904 2211 21 -0.282849117361458 2211 22 -0.21691187923166666 2211 23 -0.504975912385504 2211 24 -0.280414656848599 2211 25 -0.2508783704276354 2212 1 -0.30624927715554406 2212 2 -0.23678492286042996 2212 3 -2.300480438886229 2212 4 -1.3421859461153502 2212 5 -0.5994951697895203 2212 6 -0.37145567367242194 2212 7 -0.31593045806884845 2212 8 -0.28798757930010765 2212 9 -0.8308472068245507 2212 10 -0.19034903610579335 2212 11 -0.3357336852641666 2212 12 0.010178676204908754 2212 13 -0.32261596540105586 2212 14 0.007068138807078128 2212 15 0.0988977395366959 2212 16 -0.982049909141999 2212 17 -1.2238332228110522 2212 18 -0.2113037362380652 2212 19 -0.049144459258151585 2212 20 -0.17040134009132402 2212 21 -0.08892293690037434 2212 22 -0.2634481935729269 2212 23 -0.0860556712930105 2212 24 -0.09134838705271875 2212 25 0.023100240072395195 2213 1 -3.284270230244534 2213 2 -1.5080913818086152 2213 3 -2.466157609485162 2213 4 -1.1325864362781886 2213 5 -0.8680541237259689 2213 6 -0.7153059258912151 2213 7 -0.45815438234569683 2213 8 -0.210118836002394 2213 9 -0.27359219998648854 2213 10 -0.18017455463980525 2213 11 -0.5780541017725653 2213 12 -0.3240544507239562 2213 13 -0.7604188353926062 2213 14 -0.3432923369202996 2213 15 -0.3104867823220374 2213 16 -1.6422031319798265 2213 17 -1.0476041161059229 2213 18 -0.31315229336454564 2213 19 -0.1995846243684838 2213 20 -0.1663129930428866 2213 21 0.07675545260482863 2213 22 -0.1317571543487972 2213 23 -0.06755818183361839 2213 24 -0.06141841457201025 2213 25 -0.09438683929546698 2214 1 -1.0263725544437392 2214 2 -0.8282789511510202 2214 3 -6.5311969807582955 2214 4 -2.2320574323326596 2214 5 -1.380127167795095 2214 6 -0.7711723847795326 2214 7 -0.7404502417148819 2214 8 -0.5010386861172265 2214 9 -0.3965891725142896 2214 10 -0.4114615108602635 2214 11 -0.8016920517101198 2214 12 -0.6310522578815565 2214 13 -5.327550167803569 2214 14 -3.83646171428078 2214 15 -2.227011435229727 2214 16 -2.8667868491661608 2214 17 -3.4363872829226807 2214 18 -1.2519397307209843 2214 19 -0.8947644907949694 2214 20 -0.49407493118358303 2214 21 -0.5040114846097978 2214 22 -0.5513628443451921 2214 23 -0.3519182072034681 2214 24 -0.398214806530504 2214 25 -0.20092098909048417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1270.0 3 158.0 4 168.0 5 335.0 6 47.0 7 54.0 8 92.0 9 270.0 10 609.0 11 1490.0 12 3365.0 13 6700.0 14 11074.0 15 16233.0 16 21650.0 17 25539.0 18 26595.0 19 26100.0 20 25516.0 21 24227.0 22 23570.0 23 23752.0 24 24096.0 25 25897.0 26 28023.0 27 31765.0 28 37133.0 29 45504.0 30 57272.0 31 72849.0 32 97169.0 33 132335.0 34 182562.0 35 255227.0 36 366159.0 37 560359.0 38 1074907.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.608693226842234 21.5590562942794 13.636807361819455 22.195443117058915 2 15.090402713273985 22.450365938238882 40.466601843646 21.992629504841126 3 17.84114317930481 28.32832020420112 30.268974254923037 23.561562361571035 4 12.033157618564102 18.755254251568452 38.20786423230777 31.00372389755967 5 12.645708602309409 39.37592904576101 34.3810197286807 13.597342623248887 6 28.78275298230352 40.158197005211484 17.790781176712596 13.268268835772401 7 24.85312832637544 35.51227575815028 22.814675890988443 16.81992002448584 8 23.524227519170683 37.74529089193477 21.332787708953795 17.397693879940746 9 24.42173288061662 18.30432600560113 21.888615579072592 35.38532553470966 10 15.247950551775713 31.110596163128086 32.440421791965285 21.201031493130916 11 30.951387449711394 27.901559949654857 23.08454123564914 18.0625113649846 12 20.264818560284898 29.381552041342 31.011862406860764 19.341766991512337 13 25.981146863025113 25.6342255994994 26.95595874985673 21.42866878761876 14 20.117825351812485 24.993014245981566 28.40574870244653 26.48341169975942 15 22.636488399686485 31.50827310381576 24.637151363257566 21.21808713324019 16 20.84501597078884 32.23841037446086 26.12295044641073 20.793623208339568 17 19.32844543619169 31.082942763151983 28.526632657708102 21.06197914294822 18 19.870187571951362 30.135222829475616 29.699228188408473 20.295361410164546 19 20.676560003643317 29.55751373446363 30.008219146677224 19.75770711521583 20 22.2443926892601 28.425027369735854 29.153740778397147 20.1768391626069 21 22.504977359866448 29.010038979380237 28.54831469863081 19.9366689621225 22 21.63645747623249 28.603541086316458 29.29608889395577 20.463912543495287 23 21.456667239779613 28.890716654507376 29.29096634587831 20.361649759834695 24 21.27742833799167 28.247210652548205 29.21497347676114 21.26038753269898 25 21.55193192189656 28.342888796222343 29.102193367540092 21.002985914341004 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3584.0 1 3584.0 2 3933.5 3 4283.0 4 4283.0 5 4283.0 6 7127.0 7 9971.0 8 9971.0 9 9971.0 10 13968.0 11 17965.0 12 17965.0 13 17965.0 14 27635.5 15 37306.0 16 37306.0 17 37306.0 18 57516.5 19 77727.0 20 77727.0 21 77727.0 22 109090.5 23 140454.0 24 140454.0 25 140454.0 26 181939.0 27 223424.0 28 223424.0 29 223424.0 30 262816.0 31 302208.0 32 302208.0 33 302208.0 34 328918.5 35 355629.0 36 355629.0 37 355629.0 38 363592.5 39 371556.0 40 371556.0 41 371556.0 42 365143.0 43 358730.0 44 358730.0 45 358730.0 46 359235.5 47 359741.0 48 359741.0 49 359741.0 50 334181.0 51 308621.0 52 308621.0 53 308621.0 54 274270.5 55 239920.0 56 239920.0 57 239920.0 58 205745.5 59 171571.0 60 171571.0 61 171571.0 62 146355.5 63 121140.0 64 121140.0 65 121140.0 66 93912.0 67 66684.0 68 66684.0 69 66684.0 70 50655.0 71 34626.0 72 34626.0 73 34626.0 74 25187.0 75 15748.0 76 15748.0 77 15748.0 78 10923.5 79 6099.0 80 6099.0 81 6099.0 82 4112.5 83 2126.0 84 2126.0 85 2126.0 86 1384.5 87 643.0 88 643.0 89 643.0 90 433.5 91 224.0 92 224.0 93 224.0 94 141.5 95 59.0 96 59.0 97 59.0 98 45.5 99 32.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10092657405982716 2 0.05554057480470243 3 0.05823401405108433 4 0.05365207142505536 5 0.05027753259912863 6 0.06801708073909211 7 0.06448774655417791 8 0.06083457608207374 9 0.06659296343640744 10 0.0659118638568626 11 0.06049402629230131 12 0.06111320772825118 13 0.06071073979488377 14 0.06417815583620298 15 0.0662833727184325 16 0.061608552877011065 17 0.0649521326311403 18 0.14866546277156137 19 0.0694102389699793 20 0.06355897440025313 21 0.06049402629230131 22 0.06609761828764754 23 0.06458062376957038 24 0.07814069721687232 25 0.06962695247256175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3230071.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.83220346967943 #Duplication Level Percentage of deduplicated Percentage of total 1 82.00276145687774 54.804252387616394 2 12.063045103082722 16.12399769586289 3 3.093638340022785 6.202640010060122 4 1.1377658487192148 3.041575948098203 5 0.5247210778789422 1.7534132920817487 6 0.27154792098729064 1.0888887544314627 7 0.1895703464752804 0.8868582777217499 8 0.12106616497907693 0.6472894856940359 9 0.09537723854845837 0.573684391174205 >10 0.4432764206952121 5.244268818149379 >50 0.03001077627832582 1.3944070142941887 >100 0.022347255409105968 3.024364118108718 >500 0.0023891783898350076 1.1074178514227246 >1k 0.002342331750942986 2.866618935063042 >5k 0.0 0.0 >10k+ 1.4053990505657915E-4 1.2403230202212483 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 18056 0.5589970003755335 No Hit GGTATCAACGCAGAGTACTTTTTTT 11090 0.34333610623419736 No Hit TATCAACGCAGAGTACTTTTTTTTT 10470 0.3241414817197517 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4271 0.1322261956470926 No Hit GTACTGGTTCACTATCGGTCAGTCA 4235 0.13111166906238286 No Hit GTACATGGGAAGCAGTGGTATCAAC 3864 0.11962585342551295 No Hit CCCATGTACTCTGCGTTGATACCAC 3596 0.1113288221837848 No Hit GGTATCAACGCAGAGTACATGGGGG 3255 0.1007717787008397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.0959071797493E-5 0.0 0.0 0.0 3 0.0 3.0959071797493E-5 0.0 0.0 0.0 4 0.0 3.0959071797493E-5 0.0 3.0959071797493E-5 0.0 5 0.0 3.0959071797493E-5 0.0 3.0959071797493E-5 0.0 6 0.0 3.0959071797493E-5 0.0 3.0959071797493E-5 0.0 7 0.0 3.0959071797493E-5 0.0 3.0959071797493E-5 0.0 8 0.0 3.0959071797493E-5 0.0 3.0959071797493E-5 0.0 9 0.0 3.0959071797493E-5 0.0 3.0959071797493E-5 0.0 10 0.0 3.0959071797493E-5 0.0 6.1918143594986E-5 0.0 11 0.0 6.1918143594986E-5 0.0 6.1918143594986E-5 0.0 12 0.0 1.5479535898746497E-4 0.0 6.1918143594986E-5 0.0 13 0.0 1.5479535898746497E-4 0.0 1.23836287189972E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATTA 70 1.0859881E-4 12.221035 15 GGTATCA 4385 0.0 11.178609 1 CCGAATT 95 1.3530318E-5 11.006078 14 AATCGCC 220 0.0 10.802759 18 CTTGCAC 225 0.0 10.551735 3 TACACTG 470 0.0 10.102568 5 ATCGCCG 230 0.0 9.910529 19 GTACTAG 175 2.579327E-9 9.7710705 1 GTATCAA 8640 0.0 9.69756 1 GTGTAGG 305 0.0 9.655384 1 GTCTACG 80 0.0045190398 9.499652 1 GTCCTAT 180 4.1909516E-9 9.499651 1 GCAACCG 120 1.7089484E-5 9.496561 9 CTAGTAC 150 2.6648377E-7 9.49656 3 TAGCACG 80 0.0045317644 9.496414 4 TAGACAG 420 0.0 9.496413 5 ACGGTAC 295 0.0 9.335603 3 CGCCCTA 490 0.0 9.11728 16 AGGACCG 115 1.1158537E-4 9.083526 5 ATCCCGT 115 1.1158537E-4 9.083526 6 >>END_MODULE