FastQCFastQC Report
Wed 25 May 2016
SRR1294656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294656_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230071
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT427161.3224477109017108No Hit
GGTATCAACGCAGAGTACTTTTTTT283520.8777516036025215No Hit
TATCAACGCAGAGTACTTTTTTTTT270120.8362664473938809No Hit
ACGCAGAGTACTTTTTTTTTTTTTT147920.45794659002851634No Hit
GTACTTTTTTTTTTTTTTTTTTTTT88160.2729351769666983No Hit
GAGTACTTTTTTTTTTTTTTTTTTT73530.22764205492696601No Hit
GCAGAGTACTTTTTTTTTTTTTTTT52350.16207074085987586No Hit
ATCAACGCAGAGTACTTTTTTTTTT43840.1357245707602093No Hit
CCCATGTACTCTGCGTTGATACCAC41600.12878973867757085No Hit
GTACTGGTTCACTATCGGTCAGTCA39440.12210257916931237No Hit
GTACATGGGAAGCAGTGGTATCAAC35280.1092236053015553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67650.013.52662851
CGGTATC855.305831E-511.1807882
CGTGCGC700.001468975810.87753810
GCGGTAT1053.4163204E-610.8711181
AAATCGC1951.6370905E-1110.21206517
TGTCCCG5300.010.02481611
GTCTAGC2002.5465852E-119.987841
TATACTG5850.09.9259785
CGCCCTA5450.09.91761516
GTCCTAT2054.0017767E-119.7442341
GTATCAA135500.09.6736911
TACTCCG900.00109383459.5191765
ATACCGT900.00109383459.5191766
AATCGCC2002.746674E-109.48263218
TCTATAC4550.09.3992343
TCAGGAC3250.09.3574593
CCGTACA6550.09.26547217
GTACTGG12950.09.2551411
ATACTGT7100.09.251036
GAGGCGT1054.4050862E-49.0658826
Produced by FastQC (version 0.11.5)