##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294656_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3230071 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.204050932626558 31.0 31.0 34.0 28.0 34.0 2 31.338383273928034 31.0 31.0 34.0 28.0 34.0 3 31.47996684902592 31.0 31.0 34.0 28.0 34.0 4 35.04475412459974 37.0 35.0 37.0 32.0 37.0 5 35.198761884800675 37.0 35.0 37.0 32.0 37.0 6 34.745255444849356 37.0 35.0 37.0 32.0 37.0 7 34.58010118043845 37.0 35.0 37.0 31.0 37.0 8 34.6284914480208 37.0 35.0 37.0 31.0 37.0 9 36.00966666057805 39.0 35.0 39.0 30.0 39.0 10 35.8230478525085 39.0 35.0 39.0 30.0 39.0 11 35.966046257187536 39.0 35.0 39.0 30.0 39.0 12 35.92880435135946 39.0 35.0 39.0 30.0 39.0 13 35.761025996023 39.0 35.0 39.0 30.0 39.0 14 36.80778905479168 40.0 36.0 41.0 30.0 41.0 15 36.84650492202803 40.0 36.0 41.0 30.0 41.0 16 36.89398994635103 40.0 36.0 41.0 30.0 41.0 17 36.98506162867628 40.0 36.0 41.0 30.0 41.0 18 37.01429163631388 40.0 36.0 41.0 30.0 41.0 19 37.04927910253366 40.0 36.0 41.0 30.0 41.0 20 37.115152577141494 40.0 37.0 41.0 30.0 41.0 21 36.92733874890057 40.0 36.0 41.0 30.0 41.0 22 36.94133875075811 40.0 36.0 41.0 30.0 41.0 23 36.94799216487811 40.0 36.0 41.0 30.0 41.0 24 36.85844521683889 40.0 36.0 41.0 30.0 41.0 25 35.77738941342156 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4323777592898068 1101 2 0.42576352954483454 1101 3 0.35811620591773874 1101 4 0.2718153320193224 1101 5 0.33752770594804815 1101 6 0.3809080533958209 1101 7 0.49276758549147814 1101 8 0.41485774344613446 1101 9 0.5627808235973433 1101 10 0.6366400742700122 1101 11 0.6183796468627705 1101 12 0.7243131750945224 1101 13 0.604452273801563 1101 14 0.7623057552942782 1101 15 0.8384214017786604 1101 16 0.8907413433169467 1101 17 0.826672886748689 1101 18 0.8254739633593289 1101 19 0.9546268855837639 1101 20 0.8125468872436059 1101 21 1.1249836881925646 1101 22 1.0395819365531267 1101 23 0.9286570525603892 1101 24 1.2646050347883033 1101 25 1.0864341622718214 1102 1 0.3481977987920857 1102 2 0.31571021584022674 1102 3 0.2433390648671434 1102 4 0.20150932508646235 1102 5 0.24910618439184873 1102 6 0.3416648876571031 1102 7 0.33425200252951015 1102 8 0.3986579136540058 1102 9 0.5328573532727532 1102 10 0.643939884422764 1102 11 0.6346651554027076 1102 12 0.6192765548544799 1102 13 0.6198109637438165 1102 14 0.8675557900224362 1102 15 0.7638373332995272 1102 16 0.819918134371612 1102 17 0.7562351203997153 1102 18 0.6772145703914987 1102 19 0.7764420104534295 1102 20 0.712859616269796 1102 21 0.9847307394928322 1102 22 0.8328291547082074 1102 23 0.8597980999615231 1102 24 1.008778416895396 1102 25 0.8981101988872737 1103 1 0.2822427423543772 1103 2 0.30842527867505254 1103 3 0.24390895909282762 1103 4 0.2413104635587615 1103 5 0.23703158300636318 1103 6 0.3106347148508135 1103 7 0.2759629554471985 1103 8 0.3032370699234477 1103 9 0.2079483782574698 1103 10 0.38416086038078134 1103 11 0.46318496541756105 1103 12 0.573727409167823 1103 13 0.6302346502116976 1103 14 0.7843517534119542 1103 15 0.7593968781248677 1103 16 0.7533895413314582 1103 17 0.6575677558659052 1103 18 0.7393877684671608 1103 19 0.7589862412270776 1103 20 0.6855319347420803 1103 21 0.9536635001465967 1103 22 0.8890192133152723 1103 23 0.9059562265555456 1103 24 0.9925982753477385 1103 25 0.8738195555869126 1104 1 0.10110390528867086 1104 2 0.13289165460151509 1104 3 0.0841835048278341 1104 4 0.04341282727042284 1104 5 0.11153824620784292 1104 6 0.07344056554435952 1104 7 0.16296296119305254 1104 8 0.135221939764385 1104 9 -0.04917416003091546 1104 10 0.3367127487556374 1104 11 0.4352226352351636 1104 12 0.3255803365256469 1104 13 -0.12078769187787941 1104 14 0.22866536918494518 1104 15 0.42546456163086077 1104 16 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-0.005191717092941417 2207 1 0.19209784412535313 2207 2 0.226962348407163 2207 3 0.1986392160933903 2207 4 0.18665150198310698 2207 5 0.2275433286035451 2207 6 0.23173427178215178 2207 7 0.2277485817710243 2207 8 0.23980695629150262 2207 9 0.3133956079765099 2207 10 0.4819947757440417 2207 11 0.4157317676233063 2207 12 0.40410548232470944 2207 13 0.4230802191362244 2207 14 0.4676709572883553 2207 15 0.441650539469002 2207 16 0.46652682520910105 2207 17 0.5421962029996976 2207 18 0.5364379526686136 2207 19 0.6063041073420479 2207 20 0.47096018691529196 2207 21 0.8522928131957315 2207 22 0.7399459242110282 2207 23 0.6481400443088816 2207 24 0.8160650996421097 2207 25 0.7137206234627484 2208 1 0.18554964164887267 2208 2 0.17236461004430126 2208 3 0.20660017593708346 2208 4 0.2318130062189141 2208 5 0.17711851030084347 2208 6 0.3026614430586818 2208 7 0.3574588812167221 2208 8 0.25105598877495083 2208 9 0.3908746577818576 2208 10 0.538618091021867 2208 11 0.08518400098728307 2208 12 -0.10232187894126099 2208 13 0.11849972578357182 2208 14 -0.24409862102399416 2208 15 0.28388955807355387 2208 16 0.302593535708084 2208 17 0.2939167096173705 2208 18 0.3336781743994379 2208 19 0.4870790624215289 2208 20 0.5338861780108388 2208 21 0.7752261539932945 2208 22 0.7106989875579401 2208 23 0.6890230080527928 2208 24 0.6978768456384259 2208 25 0.7169255884879959 2209 1 -0.028024683080097645 2209 2 -0.004942564487290468 2209 3 0.09471712353227701 2209 4 -0.4871208475792912 2209 5 -0.06409049471018591 2209 6 0.036501059650824175 2209 7 0.06980465819411563 2209 8 0.1194996301979856 2209 9 0.21010228308441725 2209 10 0.3188629161770109 2209 11 0.3086249226190745 2209 12 0.26601964520890675 2209 13 0.2728561784045951 2209 14 0.31288876458815196 2209 15 -0.29316970972408996 2209 16 -0.30151014071390136 2209 17 0.07892015137324648 2209 18 -0.19117456080544315 2209 19 0.26823720274646945 2209 20 0.2429715875542513 2209 21 0.5515549767195225 2209 22 0.5151955894351445 2209 23 0.4109248313079803 2209 24 0.5845105447341936 2209 25 0.5846812928656107 2210 1 -0.11900343830097526 2210 2 -0.1723910525773249 2210 3 -0.11123340712178376 2210 4 -0.04566940193504365 2210 5 -0.07272516430991516 2210 6 -0.5564971143940411 2210 7 -0.2227854055709173 2210 8 -0.3773966486070961 2210 9 -0.1153871750310671 2210 10 0.15743218045646756 2210 11 0.06290036963594048 2210 12 -0.0026448213218728256 2210 13 0.07903172066899344 2210 14 0.023734253764502 2210 15 -0.09979004238100941 2210 16 0.12568436401149796 2210 17 -0.998003428706383 2210 18 -1.4750122163374257 2210 19 -0.37701830769830735 2210 20 -0.17554749189064722 2210 21 -0.4372034995815497 2210 22 -0.4261564827899562 2210 23 -0.1805870182804057 2210 24 0.17867101041564837 2210 25 0.19423130486589457 2211 1 -0.2805554595802384 2211 2 -0.22944277320635464 2211 3 -0.16605881766163932 2211 4 -0.06973917416258502 2211 5 -0.1594319966286193 2211 6 -0.07854381996919102 2211 7 -0.16105521448849203 2211 8 -0.14257190591228408 2211 9 -0.12267748232027031 2211 10 -0.08883834010212865 2211 11 -0.4088980849374835 2211 12 -0.2235507310940008 2211 13 -0.16825129834131758 2211 14 -0.3111650184305361 2211 15 -0.2682595816785138 2211 16 -0.2648295841895987 2211 17 -0.4157290743420887 2211 18 -0.4370181585006918 2211 19 -0.2666806880371979 2211 20 -0.39517679688751883 2211 21 0.09349494004932524 2211 22 -0.13418296164632437 2211 23 -0.7915562662408675 2211 24 -0.22209640940734232 2211 25 -0.7926427700722058 2212 1 -0.16516987604230238 2212 2 -0.2860461647903172 2212 3 -0.1752110265169975 2212 4 -0.09858629312028455 2212 5 -0.12115756205266592 2212 6 -0.15479121278183783 2212 7 -0.2818201916057106 2212 8 -0.34029941784943674 2212 9 -0.12070681405645445 2212 10 -0.5120477611315337 2212 11 -0.2343734728131608 2212 12 -0.28794471687829315 2212 13 -0.2504356871634599 2212 14 -0.27792850610731534 2212 15 -0.15572381045562622 2212 16 -0.3930131795292624 2212 17 -0.5524715517707932 2212 18 -0.3884225768305427 2212 19 -0.44329730914949295 2212 20 -0.5048529265188222 2212 21 -0.04553016654409703 2212 22 -0.33870874017812724 2212 23 -0.2683274035803578 2212 24 -0.462726295659337 2212 25 -0.2738636321987329 2213 1 -0.18758276329446488 2213 2 -0.18550937652887356 2213 3 -0.11694766818465041 2213 4 0.0023491677793217036 2213 5 -0.049042011183331624 2213 6 -0.1657173531115319 2213 7 -0.24211364283767978 2213 8 -0.21366814882264862 2213 9 -0.18175681783506548 2213 10 -0.16289386717294718 2213 11 0.10743673661931297 2213 12 0.22206494137380162 2213 13 0.23492303649902624 2213 14 -0.47516410795763164 2213 15 -0.12716695845531234 2213 16 -0.2259645136582833 2213 17 -0.1969961841960668 2213 18 -0.13903236854835654 2213 19 -0.13264708405362313 2213 20 -0.27243485936647716 2213 21 0.08562053604701703 2213 22 -0.2266907126447535 2213 23 -0.33725781694397483 2213 24 -0.5476928655749447 2213 25 -0.25423635206526995 2214 1 -0.19765485914002667 2214 2 -0.1514055188999599 2214 3 -0.14039297412033847 2214 4 -0.13922129108348713 2214 5 -0.11708898867116346 2214 6 -0.03520797985869706 2214 7 -0.07942478349926319 2214 8 0.05740661975159611 2214 9 -0.03785074867852245 2214 10 -0.10524761454781384 2214 11 0.03325931538922333 2214 12 -0.07434179809802544 2214 13 -0.059651076928751934 2214 14 -0.043797584386361166 2214 15 0.12356366581312273 2214 16 -0.15671846688092472 2214 17 -0.3327903826226617 2214 18 -0.260854274719037 2214 19 -0.4674067173095011 2214 20 -0.36765776731699873 2214 21 -0.413940300785697 2214 22 -0.8050581322967645 2214 23 -1.0089129135077286 2214 24 -0.6109748515939444 2214 25 -0.7388173767862227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 11.0 9 28.0 10 100.0 11 223.0 12 616.0 13 1442.0 14 3100.0 15 5715.0 16 8807.0 17 11886.0 18 14427.0 19 15773.0 20 16698.0 21 17849.0 22 18828.0 23 19905.0 24 21785.0 25 24221.0 26 27209.0 27 30161.0 28 35507.0 29 43710.0 30 56020.0 31 72743.0 32 98024.0 33 131072.0 34 179554.0 35 249217.0 36 355021.0 37 558771.0 38 1211646.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.46404724844261 21.82426735196204 12.863077997178207 23.848607402417148 2 14.736549134678464 22.517771281188555 39.23012837798302 23.515551206149958 3 17.290605193037713 27.902370518947254 31.088473229551116 23.718551058463913 4 11.808180920268384 18.4102907072152 38.26269231602837 31.51883605648804 5 12.535761187263676 38.767960329583524 34.33708768384556 14.359190799307243 6 28.990088120390432 40.11752068935728 17.230649757443665 13.661741432808627 7 24.440408450813266 35.59235432101633 22.941650989773247 17.025586238397164 8 24.07770225181329 37.081762198186446 21.062547203727807 17.77798834627246 9 24.782419171880186 16.63115653435969 22.36522248475572 36.221201809004405 10 15.371129393980079 30.38369783042183 32.57251403537561 21.672658740222477 11 31.386043439726475 26.681544544260188 23.25038049180638 18.682031524206963 12 20.86905667468826 28.52656832086132 31.506013409778422 19.098361594672 13 26.72341679705616 24.350647943416636 27.586618973110767 21.339316286416437 14 20.59071659417537 23.785070353552566 29.402580708677874 26.221632343594187 15 22.829804782119805 30.132518810959148 25.882746672478785 21.15492973444226 16 20.493190855424714 31.300331757721512 27.22775495748077 20.978722429373004 17 18.9458993316246 30.074292484592448 29.584581886899702 21.39522629688326 18 19.354910614774194 28.70946210353727 31.60745421762541 20.328173064063126 19 20.147731184846332 28.17613688431408 32.31913007080992 19.357001860029673 20 21.503247152298364 27.103296191619915 31.585254891471088 19.808201764610637 21 21.715837033468706 27.793831821184856 30.8492530483935 19.64107809695294 22 20.85321732044659 27.490181071992264 31.574409300845268 20.08219230671588 23 20.757971189478866 27.663982514310838 31.64024309070701 19.937803205503286 24 20.535771504713054 27.094760455729922 31.56497179164173 20.804496247915296 25 20.903507199506628 27.138404706111093 31.41086429063908 20.547223803743204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2598.0 1 2598.0 2 3123.0 3 3648.0 4 3648.0 5 3648.0 6 8661.5 7 13675.0 8 13675.0 9 13675.0 10 16500.0 11 19325.0 12 19325.0 13 19325.0 14 25060.0 15 30795.0 16 30795.0 17 30795.0 18 50933.0 19 71071.0 20 71071.0 21 71071.0 22 105828.5 23 140586.0 24 140586.0 25 140586.0 26 185542.5 27 230499.0 28 230499.0 29 230499.0 30 272360.0 31 314221.0 32 314221.0 33 314221.0 34 338119.5 35 362018.0 36 362018.0 37 362018.0 38 362642.0 39 363266.0 40 363266.0 41 363266.0 42 358822.5 43 354379.0 44 354379.0 45 354379.0 46 355253.5 47 356128.0 48 356128.0 49 356128.0 50 331114.5 51 306101.0 52 306101.0 53 306101.0 54 273037.5 55 239974.0 56 239974.0 57 239974.0 58 206125.0 59 172276.0 60 172276.0 61 172276.0 62 147554.0 63 122832.0 64 122832.0 65 122832.0 66 95321.0 67 67810.0 68 67810.0 69 67810.0 70 51298.0 71 34786.0 72 34786.0 73 34786.0 74 25064.0 75 15342.0 76 15342.0 77 15342.0 78 10599.0 79 5856.0 80 5856.0 81 5856.0 82 3953.5 83 2051.0 84 2051.0 85 2051.0 86 1325.0 87 599.0 88 599.0 89 599.0 90 386.0 91 173.0 92 173.0 93 173.0 94 112.0 95 51.0 96 51.0 97 51.0 98 31.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09423941455156867 2 0.0 3 0.13640567033975415 4 0.01117622491889497 5 0.020494905529940363 6 0.015851044760316416 7 0.024643421150804423 8 0.02222861355059997 9 2.1671350258245097E-4 10 0.3865859295352951 11 0.06228965245655591 12 0.0012383628718997198 13 1.5479535898746497E-4 14 8.668540103298039E-4 15 0.001207403800102227 16 9.2877215392479E-5 17 0.0 18 2.1671350258245097E-4 19 4.643860769623949E-4 20 3.0959071797492994E-4 21 2.7863164617743697E-4 22 0.002631521102786905 23 3.0959071797493E-5 24 0.0 25 0.001795626164254594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3230071.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.26510109122213 #Duplication Level Percentage of deduplicated Percentage of total 1 80.81223887386592 51.12594461763119 2 12.768880568904736 16.156490400270002 3 3.3660599644714004 6.388623717941962 4 1.182219883640475 2.9917304180227 5 0.5791825685585865 1.8321021875066335 6 0.3199362374418812 1.214447904270352 7 0.19499962730405593 0.8635669793899218 8 0.13341424368580448 0.6752372491033092 9 0.09207987835227374 0.5242898531181631 >10 0.48886499844662784 5.433309605329561 >50 0.03206896827803597 1.416088384527449 >100 0.0249368146901677 3.2774185921325403 >500 0.002312658663387942 1.0583236016834252 >1k 0.0024602751725497744 2.862426492402437 >5k 1.4761651035298645E-4 0.6663046257613486 >10k+ 1.9682201380398194E-4 3.51369537090894 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 42716 1.3224477109017108 No Hit GGTATCAACGCAGAGTACTTTTTTT 28352 0.8777516036025215 No Hit TATCAACGCAGAGTACTTTTTTTTT 27012 0.8362664473938809 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 14792 0.45794659002851634 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 8816 0.2729351769666983 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 7353 0.22764205492696601 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 5235 0.16207074085987586 No Hit ATCAACGCAGAGTACTTTTTTTTTT 4384 0.1357245707602093 No Hit CCCATGTACTCTGCGTTGATACCAC 4160 0.12878973867757085 No Hit GTACTGGTTCACTATCGGTCAGTCA 3944 0.12210257916931237 No Hit GTACATGGGAAGCAGTGGTATCAAC 3528 0.1092236053015553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.0959071797493E-5 0.0 8 0.0 0.0 0.0 3.0959071797493E-5 0.0 9 0.0 0.0 0.0 6.1918143594986E-5 0.0 10 0.0 0.0 0.0 6.1918143594986E-5 0.0 11 0.0 0.0 0.0 6.1918143594986E-5 0.0 12 0.0 3.0959071797493E-5 0.0 9.287721539247898E-5 0.0 13 0.0 3.0959071797493E-5 0.0 1.23836287189972E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6765 0.0 13.5266285 1 CGGTATC 85 5.305831E-5 11.180788 2 CGTGCGC 70 0.0014689758 10.877538 10 GCGGTAT 105 3.4163204E-6 10.871118 1 AAATCGC 195 1.6370905E-11 10.212065 17 TGTCCCG 530 0.0 10.024816 11 GTCTAGC 200 2.5465852E-11 9.98784 1 TATACTG 585 0.0 9.925978 5 CGCCCTA 545 0.0 9.917615 16 GTCCTAT 205 4.0017767E-11 9.744234 1 GTATCAA 13550 0.0 9.673691 1 TACTCCG 90 0.0010938345 9.519176 5 ATACCGT 90 0.0010938345 9.519176 6 AATCGCC 200 2.746674E-10 9.482632 18 TCTATAC 455 0.0 9.399234 3 TCAGGAC 325 0.0 9.357459 3 CCGTACA 655 0.0 9.265472 17 GTACTGG 1295 0.0 9.255141 1 ATACTGT 710 0.0 9.25103 6 GAGGCGT 105 4.4050862E-4 9.065882 6 >>END_MODULE