FastQCFastQC Report
Wed 25 May 2016
SRR1294655_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294655_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510616
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC259731.0345269846125413No Hit
CCCATGTACTCTGCGTTGATACCAC254171.0123810252145289No Hit
GTATCAACGCAGAGTACTTTTTTTT196290.7818399946467322No Hit
GAGTACATGGGAAGCAGTGGTATCA118390.4715575779012004No Hit
GGTATCAACGCAGAGTACTTTTTTT115660.46068375251332744No Hit
TATCAACGCAGAGTACTTTTTTTTT108590.4325233329190924No Hit
CATGTACTCTGCGTTGATACCACTG96130.38289407858469793No Hit
GCGTTGATACCACTGCTTCCCATGT80530.3207579335111383No Hit
GTATCAACGCAGAGTACATGGGAAG76560.3049450812071619No Hit
ACGCAGAGTACATGGGAAGCAGTGG66250.2638794622514953No Hit
GGTATCAACGCAGAGTACATGGGAA62620.24942085926322466No Hit
TATCAACGCAGAGTACATGGGAAGC61950.2467521914940397No Hit
GTACTGGTTCACTATCGGTCAGTCA57360.22846982573201158No Hit
ACTCTGCGTTGATACCACTGCTTCC56760.22607997399841312No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50710.20198230235129547No Hit
GTGGTATCAACGCAGAGTACATGGG43950.17505663948608627No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41520.16537773996501257No Hit
GTTAATGATAGTGTGTCGAAACACA41380.1648201078938396No Hit
GCTTCCCATGTACTCTGCGTTGATA38760.1543844219904597No Hit
ATTCCATTCCATTCCATTCCATTCC36310.14462586074493272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA35790.1425546559091474No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32310.1286935158542764No Hit
CAGTGGTATCAACGCAGAGTACATG32010.12749858998747718No Hit
GGATACCACGTGTCCCGCCCTACTC30800.12267905565805363No Hit
ATACCACTGCTTCCCATGTACTCTG30510.12152396065348106No Hit
GTACTCTGCGTTGATACCACTGCTT29790.11865613857316291No Hit
ATACAGGGTGACAGCCCCGTACACA29250.11650527201292431No Hit
GAATGGAATGGAATGGAATGGAATG28880.11503153011053861No Hit
ACTTAGATGTTTCAGTTCCCCCGGT28360.11296032527475328No Hit
GGGAAGCAGTGGTATCAACGCAGAG26820.10682637249185062No Hit
CATGGGAAGCAGTGGTATCAACGCA26380.10507381455387842No Hit
GCAGTGGTATCAACGCAGAGTACAT26100.10395855041153247No Hit
GTACATGGGGGTTAAGCGACTAAGC25790.1027237936825066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG1550.013.48176319
AATCGCC1650.013.25039118
AAATCGC1800.012.1449817
TTGAACG600.00589119511.0798344
CGCCCGC600.00589206911.07961310
TCTCGAT1053.4475925E-610.86259117
CTCGATC1252.2232598E-710.64640118
GTACAAA6050.010.5202751
GAAATCG2100.010.40998416
AGGGTGA7450.010.3255985
GACAGTA1752.3646862E-1010.3112347
GTACTGG14950.010.2939371
GTATTAG2600.010.2303931
TCGATCT1303.8522194E-710.2291719
TCTAGTA1303.8627695E-710.2271322
TGTCCCG5850.010.22672311
GTCCTAG2053.6379788E-1210.1947481
CCTAGAC856.617549E-410.0552473
TACTGGT14000.09.9714542
TCTGTCG1856.530172E-109.7534828
Produced by FastQC (version 0.11.5)