FastQCFastQC Report
Wed 25 May 2016
SRR1294655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294655_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510616
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT469601.8704572901630516No Hit
GGTATCAACGCAGAGTACTTTTTTT302811.20611833908491No Hit
CCCATGTACTCTGCGTTGATACCAC294851.1744129727525037No Hit
TATCAACGCAGAGTACTTTTTTTTT291351.1604721709731793No Hit
GTACATGGGAAGCAGTGGTATCAAC248280.9889206473630375No Hit
ACGCAGAGTACTTTTTTTTTTTTTT168160.6697957792031916No Hit
GAGTACATGGGAAGCAGTGGTATCA121350.48334751312028607No Hit
CATGTACTCTGCGTTGATACCACTG111170.4427996953735657No Hit
GTACTTTTTTTTTTTTTTTTTTTTT107240.4271461665184958No Hit
GCGTTGATACCACTGCTTCCCATGT89690.35724300331074127No Hit
GAGTACTTTTTTTTTTTTTTTTTTT85910.3421869373890711No Hit
ACGCAGAGTACATGGGAAGCAGTGG78650.31326973141252984No Hit
GTATCAACGCAGAGTACATGGGAAG74330.29606279893062104No Hit
TATCAACGCAGAGTACATGGGAAGC65510.26093197844672383No Hit
ACTCTGCGTTGATACCACTGCTTCC65180.25961755999324465No Hit
GGTATCAACGCAGAGTACATGGGAA64510.25694889222405976No Hit
GCAGAGTACTTTTTTTTTTTTTTTT59080.2353207340349938No Hit
GTACTGGTTCACTATCGGTCAGTCA54820.21835278672644484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA51050.20333655166700124No Hit
ATCAACGCAGAGTACTTTTTTTTTT50460.20098653079562945No Hit
GTGGTATCAACGCAGAGTACATGGG44830.17856175536203067No Hit
GCTTCCCATGTACTCTGCGTTGATA41910.16693114359185157No Hit
GTTAATGATAGTGTGTCGAAACACA40550.1615141463290284No Hit
CAGTGGTATCAACGCAGAGTACATG39560.15757089096859098No Hit
CATGGGAAGCAGTGGTATCAACGCA35110.13984615727773583No Hit
ATTCCATTCCATTCCATTCCATTCC35040.13956734124214934No Hit
ATACCACTGCTTCCCATGTACTCTG34660.138053768477537No Hit
GGATACCACGTGTCCCGCCCTACTC33650.13403085139264628No Hit
GTGGTATCAACGCAGAGTACTTTTT33260.13247744776580728No Hit
ATACAGGGTGACAGCCCCGTACACA31980.12737909740079725No Hit
ACTTAGATGTTTCAGTTCCCCCGGT30700.12228074703578723No Hit
GAATGGAATGGAATGGAATGGAATG30220.12036886564890847No Hit
GGGAAGCAGTGGTATCAACGCAGAG30020.11957224840437566No Hit
ACATGGGAAGCAGTGGTATCAACGC29870.11897478547097605No Hit
GTACTCTGCGTTGATACCACTGCTT29270.1165849337373776No Hit
AGCGTACACGGTGGATGCCCTGGCA29220.1163857794262444No Hit
GCAGTGGTATCAACGCAGAGTACAT28910.11515102269721852No Hit
GCAGAGTACATGGGAAGCAGTGGTA27260.1085789304298228No Hit
TACCACTGCTTCCCATGTACTCTGC25680.10228565419801357No Hit
CTGCTTCCCATGTACTCTGCGTTGA25310.10081191229562785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG250.005950980419.0451269
TAGGACT1257.2759576E-1213.7130414
AATCGCC2000.012.7993918
ATCGCCG2150.012.7886219
AAATCGC2200.012.49772117
TCACGAC550.003072681112.0891573
GGACCGT855.196367E-511.20346456
CAGCGTA1451.1714292E-911.16438610
GTGCGGG600.005934573711.06891711
CGCAAAA1553.1468517E-1011.030662
AGGGTGA7850.010.9180275
GTTACTC700.001493759310.855573
CCCGAAC803.842733E-410.66615816
CCGTACA8750.010.61875317
CAGGGTG8150.010.5161364
CCAGGAC1909.094947E-1210.4984783
GTGCTAC1553.6252459E-910.4263741
CCCGTAC9000.010.32378916
TGTCCCG5550.010.25691211
GAAATCG2500.010.23951216
Produced by FastQC (version 0.11.5)