FastQCFastQC Report
Wed 25 May 2016
SRR1294654_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294654_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366865
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71680.3028478599328648No Hit
GTACATGGGAAGCAGTGGTATCAAC65030.2747516229273744No Hit
CCCATGTACTCTGCGTTGATACCAC53790.22726264489102674No Hit
GGTATCAACGCAGAGTACTTTTTTT46710.19734965872578283No Hit
TATCAACGCAGAGTACTTTTTTTTT39110.16523967357665098No Hit
ATTCCATTCCATTCCATTCCATTCC34370.14521318283890294No Hit
GAGTACATGGGAAGCAGTGGTATCA30360.12827094067468994No Hit
GAATGGAATGGAATGGAATGGAATG25960.10968094927256097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGG600.00585799211.08820716
ACCGTAC600.005890352711.0800058
CCGTTCA600.005890352711.0800059
CGCCCTA856.569045E-410.06324816
CTCGCTC1803.965397E-1010.0249773
TATACTG6850.09.9821915
GTTAGAC1151.0302787E-59.9102753
GTGTGCG1252.5810095E-69.8770329
TAGGACT2450.09.6909664
GTCTCGC2002.6557245E-109.4995571
TCCAGTA2700.09.4973472
CTAGACA3550.09.3633834
GTGTAGG2950.09.3385461
TCTCGCT2054.2564352E-109.2657042
ACTATCG1752.6848284E-89.22560411
TAGACTG3300.09.2091595
GTAGGAC1554.279227E-79.1909813
CCTATAC3150.09.0450933
AACACGG1054.5081225E-49.044715
CCAGTAC3050.09.0302643
Produced by FastQC (version 0.11.5)