##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294654_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2366865 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.92201498606807 31.0 30.0 33.0 25.0 34.0 2 29.97396682954034 31.0 30.0 34.0 25.0 34.0 3 29.878242316312928 31.0 30.0 34.0 25.0 34.0 4 33.3640672366189 35.0 33.0 37.0 26.0 37.0 5 33.81289089153796 35.0 35.0 37.0 28.0 37.0 6 33.949121306031394 37.0 35.0 37.0 28.0 37.0 7 33.80951596309887 37.0 35.0 37.0 28.0 37.0 8 33.665667877128605 37.0 35.0 37.0 27.0 37.0 9 34.70021315115142 38.0 34.0 39.0 26.0 39.0 10 34.812843571559846 38.0 34.0 39.0 27.0 39.0 11 35.088313444155034 39.0 35.0 39.0 27.0 39.0 12 34.83720026279488 38.0 34.0 39.0 27.0 39.0 13 34.708771729693076 38.0 34.0 39.0 26.0 39.0 14 35.57807902013845 39.0 34.0 41.0 25.0 41.0 15 35.57970395438692 39.0 34.0 41.0 25.0 41.0 16 35.70750211778027 39.0 34.0 41.0 27.0 41.0 17 35.6846220633623 39.0 34.0 41.0 26.0 41.0 18 35.597271918761734 39.0 34.0 41.0 25.0 41.0 19 35.88645064251658 39.0 34.0 41.0 27.0 41.0 20 35.9799046417941 39.0 34.0 41.0 27.0 41.0 21 36.0354823785894 39.0 35.0 41.0 27.0 41.0 22 36.031259915542286 39.0 35.0 41.0 27.0 41.0 23 36.01631905495244 39.0 35.0 41.0 27.0 41.0 24 36.01999522575221 39.0 34.0 41.0 27.0 41.0 25 34.77808916013376 38.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9394659523048468 1101 2 0.9016899707085599 1101 3 1.3003273309801529 1101 4 1.0740875496848687 1101 5 0.7614284868726173 1101 6 0.8098770161928357 1101 7 0.7412363581406112 1101 8 0.8399258572995407 1101 9 1.1633020108745384 1101 10 1.1368295819599012 1101 11 0.7768356132933221 1101 12 1.0414254443040676 1101 13 1.0408786848278737 1101 14 1.2242899138067784 1101 15 1.337525598745266 1101 16 1.2747271247167618 1101 17 1.274405517648347 1101 18 1.3245859000130622 1101 19 1.2063517002681863 1101 20 0.9618778556096288 1101 21 0.885390293584571 1101 22 0.9041855047120748 1101 23 0.9824514004145897 1101 24 0.97489217166207 1101 25 1.0736925466557352 1102 1 0.8679026309305833 1102 2 0.8474434481594493 1102 3 1.1982842516111667 1102 4 1.0467513141109563 1102 5 0.8335289921449984 1102 6 0.6920460422117998 1102 7 0.549173701556775 1102 8 0.687640874539774 1102 9 1.0754143034286159 1102 10 0.9745904495101669 1102 11 0.7139940494545982 1102 12 1.0056944305473934 1102 13 1.0285539719252412 1102 14 1.134588512083738 1102 15 1.3495330758940511 1102 16 1.0325956249078274 1102 17 1.1852459985132526 1102 18 1.1653796976633473 1102 19 1.010342440980537 1102 20 0.24674908331594736 1102 21 0.6200759527182669 1102 22 0.745916270575762 1102 23 0.7828399276582374 1102 24 0.9017122042244807 1102 25 0.8602832970028658 1103 1 0.7616611673754257 1103 2 0.7325231829366565 1103 3 1.0064739430304108 1103 4 0.7847218865905958 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-1.3104832307887335 2207 10 -0.27345475597941515 2207 11 0.022917749772993545 2207 12 0.4931142166631588 2207 13 0.5638063701679101 2207 14 0.7824874284074568 2207 15 0.7978907264940034 2207 16 0.7861890991135709 2207 17 0.6805857559718973 2207 18 0.7560660576745093 2207 19 -0.36362109105212426 2207 20 0.19330076370410865 2207 21 -0.4256458847930489 2207 22 0.08966525693458038 2207 23 -0.4969046062824347 2207 24 -1.7654170005945176 2207 25 -0.33067688559448527 2208 1 0.4191423029025714 2208 2 0.15855914901903034 2208 3 0.6780906045549138 2208 4 0.6301275573446148 2208 5 0.4419227628388782 2208 6 0.31279574538154264 2208 7 0.3176340749873674 2208 8 0.4763491782192517 2208 9 -0.21632941412669737 2208 10 0.15949636869964934 2208 11 0.3397317723214144 2208 12 0.22170823492218972 2208 13 0.5130534603919443 2208 14 0.7576284270657112 2208 15 0.577953434067318 2208 16 0.4611513202695363 2208 17 0.3894179601134553 2208 18 0.7189677107552441 2208 19 -1.3756045179147236 2208 20 -0.20623962214114044 2208 21 -0.3020455145962728 2208 22 0.16253901399442583 2208 23 0.2810882871059235 2208 24 0.4973758407819915 2208 25 0.3169723336265875 2209 1 0.08864409063529166 2209 2 -1.4651017657726761 2209 3 -0.24239233857364795 2209 4 0.05965477573418099 2209 5 0.05336954594421428 2209 6 0.2208741675801491 2209 7 0.11318944049824609 2209 8 0.3541571957160201 2209 9 -1.8157722842722777 2209 10 -0.316203014765037 2209 11 -0.05127885912051511 2209 12 0.2550489773773492 2209 13 0.35641696700627534 2209 14 0.6056642228641351 2209 15 0.6200080987107981 2209 16 0.5709155892095623 2209 17 0.319311477973379 2209 18 0.49821249659486 2209 19 0.3191279284175934 2209 20 0.22978087839955919 2209 21 0.24944053700030366 2209 22 0.22279841071254225 2209 23 0.28572594021061093 2209 24 0.3698557111268741 2209 25 0.3836514162867459 2210 1 -1.3404615444301271E-5 2210 2 0.12831586995288546 2210 3 0.05009130529622752 2210 4 0.22955518099473693 2210 5 0.06981052917640795 2210 6 0.010700768690483642 2210 7 -0.06344289419870108 2210 8 0.16872713048525156 2210 9 0.2964311338592651 2210 10 -1.5582593855865952 2210 11 -0.497449931969399 2210 12 -2.432817477304795 2210 13 -0.8949468694709068 2210 14 -0.6481511506872479 2210 15 -0.8419740100091531 2210 16 -0.18386989270985055 2210 17 0.11932120063361396 2210 18 0.2086740085548371 2210 19 0.15828606581305138 2210 20 0.16020819900436578 2210 21 0.04905364733831874 2210 22 0.05034871336997071 2210 23 0.010262588078660428 2210 24 -0.034008315356103935 2210 25 0.026183501726038116 2211 1 -0.4489257361823995 2211 2 -0.13404707491032042 2211 3 -1.3783099870113134 2211 4 -0.6041314182605007 2211 5 -0.5737249601887626 2211 6 -0.6170447066758697 2211 7 -0.6081164274403221 2211 8 -0.1747328345318806 2211 9 0.039140668714587434 2211 10 0.003131113653182638 2211 11 -0.29616722366572645 2211 12 -0.2748972894337598 2211 13 -0.2514138004651798 2211 14 -0.680706492205907 2211 15 -0.34621849846253383 2211 16 -0.030768412153150848 2211 17 -0.1844711738093281 2211 18 -0.032308714006120454 2211 19 -0.17648270811596944 2211 20 -0.12238054804130627 2211 21 -0.20706171046342092 2211 22 -0.261260143391965 2211 23 -0.6198567575620899 2211 24 -0.4240121121237266 2211 25 -0.3132555155824548 2212 1 -0.4580902321788187 2212 2 -0.3767970475450788 2212 3 -2.5993024779496388 2212 4 -1.573250627252122 2212 5 -0.63564018350678 2212 6 -0.5708869390772193 2212 7 -0.2914475581954008 2212 8 -0.42532981210522536 2212 9 -0.9860423090459989 2212 10 -0.21174088146312897 2212 11 -0.42942039184158176 2212 12 0.02487827934928788 2212 13 -0.4650493742109063 2212 14 -0.14902676160254913 2212 15 0.033675021645571235 2212 16 -1.1688018913749048 2212 17 -1.4960323630509436 2212 18 -0.399719916504246 2212 19 -0.29956146154712826 2212 20 -0.18881727744773968 2212 21 -0.11294970189890563 2212 22 -0.5156771014516082 2212 23 -0.20153804051234658 2212 24 -0.23858878953836182 2212 25 -0.16906629284858354 2213 1 -2.969625003244264 2213 2 -1.2928838861507153 2213 3 -2.3426432189992745 2213 4 -1.0867239249257352 2213 5 -0.8371028244585901 2213 6 -0.6096666535214723 2213 7 -0.3395187262495014 2213 8 -0.06924420727345648 2213 9 -0.22160631771123462 2213 10 -0.13496296106406191 2213 11 -0.3151586761389993 2213 12 -0.1691166814555629 2213 13 -0.43894830966495135 2213 14 -0.14566660484833704 2213 15 -0.39686442264278554 2213 16 -1.8229612768986954 2213 17 -0.9946781034517258 2213 18 -0.3195020904807677 2213 19 -0.15716325879851212 2213 20 -0.1869771179256432 2213 21 0.012589427350981452 2213 22 -0.017621766490627522 2213 23 0.07849149294966651 2213 24 0.10074031953993057 2213 25 -0.03192086205807243 2214 1 -1.0312388336272704 2214 2 -0.8335733166472217 2214 3 -6.788290372979656 2214 4 -2.257183037655622 2214 5 -1.2821248385366175 2214 6 -0.791767098629407 2214 7 -0.5896462506289168 2214 8 -0.41512797826712955 2214 9 -0.42822452095305863 2214 10 -0.2358205989214568 2214 11 -0.8806086395667592 2214 12 -0.5522019975537589 2214 13 -5.2299200412754345 2214 14 -3.822041767010248 2214 15 -2.0952284908077914 2214 16 -2.786153954794166 2214 17 -3.468565354425529 2214 18 -1.287058473731264 2214 19 -0.7425477999312591 2214 20 -0.408837084923249 2214 21 -0.44407350303859516 2214 22 -0.28302912470972785 2214 23 -0.11562120781282204 2214 24 -0.22670962558089514 2214 25 0.031552369472109376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 891.0 3 117.0 4 128.0 5 261.0 6 44.0 7 83.0 8 210.0 9 511.0 10 1023.0 11 2172.0 12 4286.0 13 7145.0 14 10543.0 15 13775.0 16 16451.0 17 18483.0 18 19610.0 19 19972.0 20 20276.0 21 20228.0 22 20415.0 23 20862.0 24 21969.0 25 23193.0 26 25671.0 27 28836.0 28 32942.0 29 40058.0 30 48887.0 31 60271.0 32 77835.0 33 102422.0 34 135528.0 35 184008.0 36 257399.0 37 385853.0 38 744507.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03250019666074 21.602315606235287 13.392723467928228 21.97246072917574 2 14.678171531377979 21.49498141703951 42.08744644083043 21.739400610752078 3 18.520376997169773 28.20030141173138 29.324571397168082 23.95475019393076 4 11.776221151248892 17.579894295749554 39.11437710538764 31.52950744761392 5 12.5211510928822 39.23330643521605 34.606844352050565 13.638698119851192 6 29.853479853479854 39.183442749924744 17.958991608576095 13.004085788019307 7 25.083391746309836 34.12707337881795 23.56162449235671 17.22791038251551 8 23.704487210380844 38.23839515285567 21.33853660681296 16.71858102995053 9 24.362278547801207 17.790838863296603 22.685701916377486 35.16118067252471 10 14.754796287519781 31.308504265175223 33.077314307287935 20.859385140017057 11 31.400171128926708 27.541061641924557 22.5794219156632 18.479345313485535 12 20.256274215947595 29.28349331095549 31.888553268214935 18.571679204881978 13 25.788639965706068 25.367659959085714 27.24287688554273 21.600823189665487 14 19.84906352758939 24.255020153329337 28.913242179011622 26.98267414006966 15 22.687767857029613 31.19235141743059 24.660793700252103 21.45908702528769 16 20.798012029958215 32.716235708717406 26.288646283040535 20.197105978283844 17 18.833838050959923 31.42373493298452 29.31508204497518 20.427344971080377 18 19.06123022299844 30.978624168854 30.461617089833997 19.49852851831356 19 19.833445514013 29.487297651985088 30.961106563753578 19.71815027024833 20 21.840822058884456 28.850483939564842 29.684980582384718 19.623713419165988 21 22.172482847807238 29.721307781508816 28.639268533859013 19.466940836824932 22 20.25343695277007 29.383694904964315 30.47348310293482 19.889385039330797 23 20.09376376671771 29.874950640635927 29.966735805007893 20.06454978763847 24 20.068699985539514 28.78993651762784 30.22951579363958 20.91184770319306 25 20.853819559979318 28.97052985214402 29.137787939913927 21.037862647962736 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2589.0 1 2589.0 2 2677.5 3 2766.0 4 2766.0 5 2766.0 6 4448.5 7 6131.0 8 6131.0 9 6131.0 10 9437.0 11 12743.0 12 12743.0 13 12743.0 14 19955.5 15 27168.0 16 27168.0 17 27168.0 18 41525.5 19 55883.0 20 55883.0 21 55883.0 22 78219.5 23 100556.0 24 100556.0 25 100556.0 26 128934.0 27 157312.0 28 157312.0 29 157312.0 30 186784.5 31 216257.0 32 216257.0 33 216257.0 34 239330.5 35 262404.0 36 262404.0 37 262404.0 38 277454.0 39 292504.0 40 292504.0 41 292504.0 42 287895.5 43 283287.0 44 283287.0 45 283287.0 46 282911.0 47 282535.0 48 282535.0 49 282535.0 50 259578.0 51 236621.0 52 236621.0 53 236621.0 54 206571.5 55 176522.0 56 176522.0 57 176522.0 58 148978.0 59 121434.0 60 121434.0 61 121434.0 62 97464.0 63 73494.0 64 73494.0 65 73494.0 66 54362.0 67 35230.0 68 35230.0 69 35230.0 70 24683.5 71 14137.0 72 14137.0 73 14137.0 74 9574.5 75 5012.0 76 5012.0 77 5012.0 78 3287.5 79 1563.0 80 1563.0 81 1563.0 82 1020.5 83 478.0 84 478.0 85 478.0 86 315.0 87 152.0 88 152.0 89 152.0 90 101.0 91 50.0 92 50.0 93 50.0 94 31.0 95 12.0 96 12.0 97 12.0 98 18.5 99 25.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1008507033565497 2 0.053108225437445734 3 0.05619247401098078 4 0.05103797639493592 5 0.048502977567372874 6 0.06730421887179877 7 0.06434672023964189 8 0.06045972203737855 9 0.06629021934077355 10 0.06531846979020772 11 0.059023222701759505 12 0.06041747205691917 13 0.06050197201783794 14 0.06333272070861667 15 0.06607896943847663 16 0.06071322192013486 17 0.0640509703764262 18 0.13777718627805136 19 0.06899421809017413 20 0.06282572094310407 21 0.06016397217416287 22 0.06519171984882957 23 0.06586771953617972 24 0.07600771484643189 25 0.07005046760165873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2366865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.72845366942947 #Duplication Level Percentage of deduplicated Percentage of total 1 92.41463143266888 76.45319554854947 2 5.953859058105664 9.851071064856148 3 0.8053243207523575 1.9986970727467845 4 0.24854920106135392 0.8224836425831171 5 0.13747610430525084 0.5686592762835299 6 0.09159144367525625 0.45463311027627573 7 0.05418871320296946 0.3138063914732502 8 0.043379036199131714 0.2870944469139497 9 0.030604091280709792 0.2278646232850071 >10 0.18978255516061615 2.9788655681015115 >50 0.016103299688668457 0.9330148622072154 >100 0.011993539296381492 1.8815080402925515 >500 0.001438402630183633 0.7529099135296347 >1k 9.246874051123641E-4 1.6665930378705445 >5k 1.5411456751872733E-4 0.8096034010310819 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7168 0.3028478599328648 No Hit GTACATGGGAAGCAGTGGTATCAAC 6503 0.2747516229273744 No Hit CCCATGTACTCTGCGTTGATACCAC 5379 0.22726264489102674 No Hit GGTATCAACGCAGAGTACTTTTTTT 4671 0.19734965872578283 No Hit TATCAACGCAGAGTACTTTTTTTTT 3911 0.16523967357665098 No Hit ATTCCATTCCATTCCATTCCATTCC 3437 0.14521318283890294 No Hit GAGTACATGGGAAGCAGTGGTATCA 3036 0.12827094067468994 No Hit GAATGGAATGGAATGGAATGGAATG 2596 0.10968094927256097 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.224998045938404E-5 0.0 0.0 0.0 3 0.0 4.224998045938404E-5 0.0 0.0 0.0 4 0.0 4.224998045938404E-5 0.0 0.0 0.0 5 0.0 4.224998045938404E-5 0.0 4.224998045938404E-5 0.0 6 0.0 4.224998045938404E-5 0.0 4.224998045938404E-5 4.224998045938404E-5 7 0.0 4.224998045938404E-5 0.0 4.224998045938404E-5 4.224998045938404E-5 8 0.0 4.224998045938404E-5 0.0 4.224998045938404E-5 4.224998045938404E-5 9 0.0 4.224998045938404E-5 0.0 4.224998045938404E-5 4.224998045938404E-5 10 0.0 4.224998045938404E-5 0.0 4.224998045938404E-5 4.224998045938404E-5 11 0.0 4.224998045938404E-5 0.0 1.267499413781521E-4 4.224998045938404E-5 12 0.0 4.224998045938404E-5 0.0 1.267499413781521E-4 4.224998045938404E-5 13 0.0 8.449996091876808E-5 0.0 1.267499413781521E-4 4.224998045938404E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGGGG 60 0.005857992 11.088207 16 ACCGTAC 60 0.0058903527 11.080005 8 CCGTTCA 60 0.0058903527 11.080005 9 CGCCCTA 85 6.569045E-4 10.063248 16 CTCGCTC 180 3.965397E-10 10.024977 3 TATACTG 685 0.0 9.982191 5 GTTAGAC 115 1.0302787E-5 9.910275 3 GTGTGCG 125 2.5810095E-6 9.877032 9 TAGGACT 245 0.0 9.690966 4 GTCTCGC 200 2.6557245E-10 9.499557 1 TCCAGTA 270 0.0 9.497347 2 CTAGACA 355 0.0 9.363383 4 GTGTAGG 295 0.0 9.338546 1 TCTCGCT 205 4.2564352E-10 9.265704 2 ACTATCG 175 2.6848284E-8 9.225604 11 TAGACTG 330 0.0 9.209159 5 GTAGGAC 155 4.279227E-7 9.190981 3 CCTATAC 315 0.0 9.045093 3 AACACGG 105 4.5081225E-4 9.04471 5 CCAGTAC 305 0.0 9.030264 3 >>END_MODULE