FastQCFastQC Report
Wed 25 May 2016
SRR1294654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366865
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT167440.7074336728119264No Hit
GGTATCAACGCAGAGTACTTTTTTT125960.5321807538664013No Hit
TATCAACGCAGAGTACTTTTTTTTT102560.43331579959144273No Hit
GTACATGGGAAGCAGTGGTATCAAC64280.2715828743929206No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64130.27094912468602983No Hit
CCCATGTACTCTGCGTTGATACCAC61990.2619076288677216No Hit
ATTCCATTCCATTCCATTCCATTCC35090.14825518143197858No Hit
GAGTACATGGGAAGCAGTGGTATCA32610.13777718627805136No Hit
GAATGGAATGGAATGGAATGGAATG29360.12404594262875154No Hit
CATGTACTCTGCGTTGATACCACTG24300.10266745251630321No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23680.1000479537278214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTG803.702693E-410.7090427
CTAGGAC2150.010.6060093
ATCGCGC1002.3470771E-510.4710648
GTCCTAA2053.6379788E-1210.2070141
ATACCGT750.002604567210.1537586
CCGGATG856.702852E-410.04119719
TGTCCCG951.6640441E-49.98838411
TAGGACT2600.09.885274
GTCTTGC4150.09.8548561
TAGACAG4050.09.6366695
TACACTG5550.09.6049075
GGTATCA51600.09.584811
TATTCCG1002.7010147E-49.5191495
TCTTAGG2500.09.5012172
CTTAGCC1901.0495569E-99.5012173
TAGACTG3650.09.388755
GTCTTAC2151.03682396E-109.2898931
TGTTAGG2600.09.1357862
AGTACCA2401.0913936E-119.1225185
TAACACG1151.0868357E-49.1052724
Produced by FastQC (version 0.11.5)