##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294654_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2366865 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19880643805202 31.0 31.0 34.0 28.0 34.0 2 31.340146565182213 31.0 31.0 34.0 28.0 34.0 3 31.483784246249787 31.0 31.0 34.0 28.0 34.0 4 35.05211366089743 37.0 35.0 37.0 32.0 37.0 5 35.17725725801852 37.0 35.0 37.0 32.0 37.0 6 34.77699995563752 37.0 35.0 37.0 32.0 37.0 7 34.60188815162673 37.0 35.0 37.0 31.0 37.0 8 34.659258132593116 37.0 35.0 37.0 31.0 37.0 9 36.06099460679 39.0 35.0 39.0 30.0 39.0 10 35.85603192408524 39.0 35.0 39.0 30.0 39.0 11 36.001967581589994 39.0 35.0 39.0 30.0 39.0 12 35.93457548275884 39.0 35.0 39.0 30.0 39.0 13 35.78814634548232 39.0 35.0 39.0 30.0 39.0 14 36.86877198319296 40.0 36.0 41.0 30.0 41.0 15 36.91878286256293 40.0 36.0 41.0 30.0 41.0 16 36.96268397225866 40.0 36.0 41.0 30.0 41.0 17 37.009578493069945 40.0 36.0 41.0 30.0 41.0 18 37.026156117902794 40.0 36.0 41.0 30.0 41.0 19 37.058878305268784 40.0 36.0 41.0 30.0 41.0 20 37.114449704567015 40.0 37.0 41.0 30.0 41.0 21 36.96382429923126 40.0 36.0 41.0 30.0 41.0 22 36.95033683796921 40.0 36.0 41.0 30.0 41.0 23 36.96759130748902 40.0 36.0 41.0 30.0 41.0 24 36.89933308405845 40.0 36.0 41.0 30.0 41.0 25 35.81397164603811 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5101344375851227 1101 2 0.4654638014042227 1101 3 0.4028677521981656 1101 4 0.3365313343159144 1101 5 0.3768698482770816 1101 6 0.43279071311052775 1101 7 0.5100665765966284 1101 8 0.4379908208428631 1101 9 0.6981679715431142 1101 10 0.6195960546573858 1101 11 0.7166172887120936 1101 12 0.6993499047913829 1101 13 0.7789608079982031 1101 14 0.9508522326230278 1101 15 0.8425012258327271 1101 16 0.9113051086479302 1101 17 0.8351655154138413 1101 18 0.7805856597020977 1101 19 0.8938104900060821 1101 20 0.9417259493264467 1101 21 1.1943189983571543 1101 22 1.1391078253508553 1101 23 1.070562877659249 1101 24 1.15502891168547 1101 25 1.0379051530925594 1102 1 0.4239751555607576 1102 2 0.46002959349797834 1102 3 0.3355965603403739 1102 4 0.33766144341158366 1102 5 0.37151344078256443 1102 6 0.3695209255180174 1102 7 0.5513286901899761 1102 8 0.4486203948319414 1102 9 0.6706316212420376 1102 10 0.744875144468736 1102 11 0.6400886886773378 1102 12 0.7590982469204945 1102 13 0.7314477552851386 1102 14 0.9860402262142145 1102 15 0.8350258576013445 1102 16 0.943310605422603 1102 17 0.8278389225406784 1102 18 0.8551144478822863 1102 19 0.9915441783559302 1102 20 0.8641281747842271 1102 21 1.259821553900366 1102 22 1.14944400325453 1102 23 1.0387849953122625 1102 24 1.0635550652360806 1102 25 1.0428839837637511 1103 1 0.25585950489089626 1103 2 0.2343858006012347 1103 3 0.26081608993644423 1103 4 0.2539493050191197 1103 5 0.19837459108651245 1103 6 0.23837744950096607 1103 7 0.30039296957367867 1103 8 0.31006019320574296 1103 9 0.2141780015197412 1103 10 0.3545361383554706 1103 11 0.355579018636746 1103 12 0.41192181051157206 1103 13 0.49593516406729776 1103 14 0.7257756653776042 1103 15 0.6486578383017161 1103 16 0.6735224428712812 1103 17 0.6655278704878214 1103 18 0.6274591002156171 1103 19 0.7476483600646802 1103 20 0.6273603231795377 1103 21 0.872199352953281 1103 22 0.8047623478308594 1103 23 0.8631470006397635 1103 24 0.9634726642563933 1103 25 0.8392536824644807 1104 1 0.1641588189444576 1104 2 0.16769826441485947 1104 3 0.08657278993546313 1104 4 0.06326821614048583 1104 5 0.16427000142771675 1104 6 0.11206178656661336 1104 7 0.28543482310104906 1104 8 0.23799224623656556 1104 9 0.05164304180314616 1104 10 0.37491821739141784 1104 11 0.31790863601798236 1104 12 0.4578095174485597 1104 13 -0.03710262677300591 1104 14 0.3054117899274189 1104 15 0.41655765479386986 1104 16 0.48208323107182594 1104 17 0.5355231110597387 1104 18 0.4968825224897415 1104 19 0.6569389599488886 1104 20 0.4808531055313807 1104 21 0.6996894661001036 1104 22 0.6574252233104829 1104 23 0.6120073874782292 1104 24 0.6249402363592722 1104 25 0.6461783559284626 1105 1 0.017327730143243514 1105 2 0.05943429441570913 1105 3 0.014712858744314161 1105 4 -0.055619301317797465 1105 5 0.06850294471718854 1105 6 0.049101707769118264 1105 7 0.020607690848471805 1105 8 -0.07403516931855592 1105 9 0.3002314916666151 1105 10 0.24975981252445933 1105 11 0.36022983106558826 1105 12 -0.25097077698598724 1105 13 -0.33472572005878476 1105 14 -0.6650673391395969 1105 15 -0.02965644903555642 1105 16 0.17888301605409396 1105 17 0.21887724082151294 1105 18 -0.5117256307159579 1105 19 -0.41763797201483754 1105 20 -0.34373504181451864 1105 21 0.2427933558725428 1105 22 0.28230532789739726 1105 23 0.33376268808620324 1105 24 0.46102196691855113 1105 25 0.2019399458845612 1106 1 -0.3913756207763228 1106 2 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2207 3 0.2230165159357682 2207 4 0.27299079482104815 2207 5 0.1828464117119566 2207 6 0.38124510250728605 2207 7 0.3044732787690023 2207 8 0.29866999822372264 2207 9 0.4570705298854989 2207 10 0.6817105577643474 2207 11 0.4541056049098131 2207 12 0.3896883971128702 2207 13 0.4378972695871184 2207 14 0.5735215381428347 2207 15 0.4982157438480357 2207 16 0.5898787771201555 2207 17 0.5211429377814127 2207 18 0.5344537183694698 2207 19 0.6296198671105486 2207 20 0.5621130290489305 2207 21 0.8476986478388326 2207 22 0.6880841109569502 2207 23 0.6187062917975226 2207 24 0.7912843945943635 2207 25 0.6879792833357072 2208 1 0.19589141306143176 2208 2 0.2043168344511912 2208 3 0.1756041162291453 2208 4 0.16534116419192202 2208 5 0.11516991886783501 2208 6 0.2396162939710038 2208 7 0.19451275940641466 2208 8 0.19136803823434434 2208 9 0.26447385747189855 2208 10 0.2310463341743798 2208 11 -0.04092461662373381 2208 12 -0.16434126329356502 2208 13 0.062205865095080526 2208 14 -0.4711151345861566 2208 15 0.19991710996503542 2208 16 0.21381847086605887 2208 17 0.1685093690248891 2208 18 0.26060496826810464 2208 19 0.48986195211952577 2208 20 0.4647082518437884 2208 21 0.6768740949180341 2208 22 0.6865903120964632 2208 23 0.63486603899036 2208 24 0.6335895729487859 2208 25 0.5589220301786639 2209 1 -0.010393039300442553 2209 2 0.030857514308074485 2209 3 0.08279855405041658 2209 4 -0.46328493959288863 2209 5 -0.12694964147146948 2209 6 0.02802479058127716 2209 7 0.024724470585539393 2209 8 0.041061561344271524 2209 9 0.1540766174564041 2209 10 0.23771166111341557 2209 11 0.15136882513290573 2209 12 0.19994905239311578 2209 13 0.21770391029678393 2209 14 0.2959790263255613 2209 15 -0.35979332891689353 2209 16 -0.352043126068601 2209 17 0.026747826944522046 2209 18 -0.29487912641182135 2209 19 0.19648339649994284 2209 20 0.16798148364463827 2209 21 0.46652547207781225 2209 22 0.44826831614631146 2209 23 0.38591740560644894 2209 24 0.4884945812079451 2209 25 0.5592938548243609 2210 1 -0.20378657093083774 2210 2 -0.1654104108559764 2210 3 -0.12579385573998536 2210 4 -0.018834795219305533 2210 5 -0.05404633447239604 2210 6 -0.5857947115780107 2210 7 -0.17248881595807575 2210 8 -0.28053070568859084 2210 9 -0.12206014338473636 2210 10 0.05801215296201434 2210 11 -0.015567974140921592 2210 12 -0.022246627240647854 2210 13 0.04415178305534084 2210 14 0.1661838899982726 2210 15 0.07854426527247682 2210 16 0.15642303004685232 2210 17 -0.8807558562616222 2210 18 -1.5693200140026349 2210 19 -0.37710547253362137 2210 20 -0.19060761920834324 2210 21 -0.4465225052874118 2210 22 -0.297866552122656 2210 23 -0.15865328566324877 2210 24 0.2544468114179921 2210 25 0.2479587741716358 2211 1 -0.26973556601129545 2211 2 -0.29433921365394866 2211 3 -0.2222743608406894 2211 4 -0.14667201590868473 2211 5 -0.1135212333812845 2211 6 -0.09638255112589889 2211 7 -0.2589459275997612 2211 8 -0.07339154954279792 2211 9 -0.09602292117921252 2211 10 -0.015635731502264605 2211 11 -0.32154337579957115 2211 12 -0.2257488508203025 2211 13 -0.05050947942058315 2211 14 -0.23195117281736088 2211 15 -0.22917228816855584 2211 16 -0.15153446332661247 2211 17 -0.3563292690972162 2211 18 -0.36088518921337 2211 19 -0.19838202125084337 2211 20 -0.24783710956192806 2211 21 0.11140742795542735 2211 22 -0.0922465596979194 2211 23 -0.7440586826264024 2211 24 -0.12928729538306527 2211 25 -0.7375419940869818 2212 1 -0.17906730505458768 2212 2 -0.3276134193895004 2212 3 -0.19451801199535979 2212 4 -0.09418888132484682 2212 5 -0.04700884215240109 2212 6 -0.19091637314350862 2212 7 -0.3662761096608378 2212 8 -0.44633939160436853 2212 9 -0.3314267440664267 2212 10 -0.5642321805533967 2212 11 -0.2748491673282203 2212 12 -0.32786396813451546 2212 13 -0.3012577806388421 2212 14 -0.33411867882166035 2212 15 -0.22757136705467218 2212 16 -0.5153146708988103 2212 17 -0.7270710886032603 2212 18 -0.37239022840179814 2212 19 -0.5855198590812449 2212 20 -0.7063335097346553 2212 21 -0.319877562317906 2212 22 -0.5227820778536909 2212 23 -0.37838132848991535 2212 24 -0.5874551677702016 2212 25 -0.40808999475350305 2213 1 -0.21784458441883814 2213 2 -0.2843341844023506 2213 3 -0.13371751394032927 2213 4 -0.017277335910449665 2213 5 -0.07136791133900999 2213 6 -0.264012198919211 2213 7 -0.3918141126862196 2213 8 -0.3936561820187592 2213 9 -0.4177881754835653 2213 10 -0.3470910740591293 2213 11 -0.12770504437859387 2213 12 0.019862159228893006 2213 13 0.011832631142318917 2213 14 -0.7234064473437627 2213 15 -0.30521668324423246 2213 16 -0.3234915985360516 2213 17 -0.21377884927426294 2213 18 -0.1776767668026551 2213 19 -0.2890592174315785 2213 20 -0.2727572162937122 2213 21 0.027354497458439653 2213 22 -0.4194559513425631 2213 23 -0.5515344395227615 2213 24 -0.5985702933812647 2213 25 -0.38944915292199056 2214 1 -0.31332560304946355 2214 2 -0.19600264431559467 2214 3 -0.2247258177953526 2214 4 -0.20648877341185568 2214 5 -0.16773494356169039 2214 6 -0.13448695574915348 2214 7 -0.08350281007357552 2214 8 -0.07000097132684857 2214 9 -0.22263302012849806 2214 10 -0.06420788306213865 2214 11 0.05404840127604871 2214 12 -0.3085393299562895 2214 13 -0.22980006369537875 2214 14 -0.24562169351782615 2214 15 -0.18692648352399033 2214 16 -0.41629626946490106 2214 17 -0.6187317879934895 2214 18 -0.45789089126145655 2214 19 -0.5027940299644982 2214 20 -0.5578183073365537 2214 21 -0.4558210218549519 2214 22 -0.8861130068732948 2214 23 -1.1782770300026613 2214 24 -0.5762360521908505 2214 25 -0.7747545998148482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 24.0 10 86.0 11 184.0 12 488.0 13 1244.0 14 2594.0 15 4489.0 16 6504.0 17 8307.0 18 9838.0 19 11121.0 20 11923.0 21 12525.0 22 13377.0 23 14656.0 24 16028.0 25 18013.0 26 20096.0 27 22507.0 28 26234.0 29 32243.0 30 40841.0 31 53063.0 32 70739.0 33 95240.0 34 129822.0 35 180031.0 36 256112.0 37 405443.0 38 903086.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.65542819323831 22.054134975863185 12.505344354015085 23.78509247688342 2 14.43859282215082 21.902854619929737 40.542996748864 23.115555809055436 3 17.972991727256726 27.702138930636227 30.00578821235631 24.319081129750735 4 11.540423129692643 17.668996447650915 39.078966772204716 31.711613650451724 5 12.62601099325082 38.47177163585776 34.78953549352782 14.112681877363597 6 29.81989683398571 39.51287082021081 17.393027694754693 13.274204651048796 7 25.023412275808443 34.24024206771811 23.378636509626922 17.357709146846528 8 23.6570150141825 38.34655512243608 20.904400801928414 17.092029061453005 9 24.103887719737052 16.9649024890752 22.862751245002354 36.0684585461854 10 14.680225160574507 31.049960471357853 33.08199563658079 21.187818731486853 11 31.727226146408118 26.748000750828194 22.463883303711697 19.060889799051996 12 20.357904120904077 28.968136773370347 31.930362699554472 18.743596406171108 13 26.23234795070608 24.62976326480947 27.383280154668793 21.754608629815657 14 19.92672107660529 23.712348115446414 29.36015720492284 27.000773603025458 15 22.888245272695393 30.485183800307166 25.08290607499044 21.543664852007005 16 20.664525154180872 32.19214631349597 26.594061422228492 20.549267110094668 17 18.57132475714701 31.09920975603161 29.69870681205173 20.630758674769652 18 18.72404789467903 30.353210582797463 31.158412411380475 19.764329111143034 19 19.513320418868076 28.90523500594671 31.95869624459462 19.622748330590596 20 21.487624954581175 28.17864174475888 30.789527052719638 19.54420624794031 21 21.871612069672114 29.133222187389357 29.583946311946047 19.411219430992478 22 19.93815262333773 28.761565222828857 31.485756681823595 19.814525472009816 23 19.704849961806 29.27760107889596 31.091900506323977 19.925648452974063 24 19.665633654644434 28.324555899892896 31.330473009656234 20.679337435806435 25 20.393997175111405 28.461745490466317 30.132130931854107 21.012126402568175 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1539.0 1 1539.0 2 1729.0 3 1919.0 4 1919.0 5 1919.0 6 3823.0 7 5727.0 8 5727.0 9 5727.0 10 8572.0 11 11417.0 12 11417.0 13 11417.0 14 17291.5 15 23166.0 16 23166.0 17 23166.0 18 37474.0 19 51782.0 20 51782.0 21 51782.0 22 75400.0 23 99018.0 24 99018.0 25 99018.0 26 128955.0 27 158892.0 28 158892.0 29 158892.0 30 189752.0 31 220612.0 32 220612.0 33 220612.0 34 243657.0 35 266702.0 36 266702.0 37 266702.0 38 279170.0 39 291638.0 40 291638.0 41 291638.0 42 286703.5 43 281769.0 44 281769.0 45 281769.0 46 283167.0 47 284565.0 48 284565.0 49 284565.0 50 261687.0 51 238809.0 52 238809.0 53 238809.0 54 208764.0 55 178719.0 56 178719.0 57 178719.0 58 150694.0 59 122669.0 60 122669.0 61 122669.0 62 98320.0 63 73971.0 64 73971.0 65 73971.0 66 54178.5 67 34386.0 68 34386.0 69 34386.0 70 23924.5 71 13463.0 72 13463.0 73 13463.0 74 8953.0 75 4443.0 76 4443.0 77 4443.0 78 2847.5 79 1252.0 80 1252.0 81 1252.0 82 785.0 83 318.0 84 318.0 85 318.0 86 196.5 87 75.0 88 75.0 89 75.0 90 44.0 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09379495661983256 2 0.0 3 0.14538218276074047 4 0.011069494880358617 5 0.021293990151529555 6 0.016392992418241006 7 0.02471623856873966 8 0.024040238881389515 9 8.449996091876808E-5 10 0.38367207255166647 11 0.06240322113851022 12 0.0012674994137815213 13 4.224998045938404E-5 14 7.604996482689127E-4 15 0.0012674994137815213 16 8.449996091876808E-5 17 4.224998045938404E-5 18 2.112499022969202E-4 19 4.224998045938404E-4 20 2.112499022969202E-4 21 2.9574986321568826E-4 22 0.0029997486126162667 23 4.224998045938404E-5 24 0.0 25 0.0019434991011316655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2366865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.02225168597347 #Duplication Level Percentage of deduplicated Percentage of total 1 91.57021306862993 73.2765463711612 2 6.606875829145227 10.57394160915668 3 0.906568989325889 2.176370755036046 4 0.29668736367921245 0.9496636355354353 5 0.15270761652552103 0.6110003661985183 6 0.09218110789235193 0.4425923889872196 7 0.06033942208976096 0.3379947494737128 8 0.04716490832825669 0.30193937319916986 9 0.03181159759772601 0.22910721025483255 >10 0.20132131367402037 3.123945430251429 >50 0.01810129483846499 1.0163476871377584 >100 0.013112628900771174 1.963621943317555 >500 0.0015899232943161754 0.8443356597860265 >1k 0.0010069514197307559 1.6658548754995468 >5k 1.5899232943117198E-4 0.8074815893460127 >10k+ 1.5899232943117198E-4 1.6792563556588609 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 16744 0.7074336728119264 No Hit GGTATCAACGCAGAGTACTTTTTTT 12596 0.5321807538664013 No Hit TATCAACGCAGAGTACTTTTTTTTT 10256 0.43331579959144273 No Hit GTACATGGGAAGCAGTGGTATCAAC 6428 0.2715828743929206 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6413 0.27094912468602983 No Hit CCCATGTACTCTGCGTTGATACCAC 6199 0.2619076288677216 No Hit ATTCCATTCCATTCCATTCCATTCC 3509 0.14825518143197858 No Hit GAGTACATGGGAAGCAGTGGTATCA 3261 0.13777718627805136 No Hit GAATGGAATGGAATGGAATGGAATG 2936 0.12404594262875154 No Hit CATGTACTCTGCGTTGATACCACTG 2430 0.10266745251630321 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2368 0.1000479537278214 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.224998045938404E-5 0.0 2 0.0 0.0 0.0 4.224998045938404E-5 0.0 3 0.0 0.0 0.0 4.224998045938404E-5 0.0 4 0.0 0.0 0.0 4.224998045938404E-5 0.0 5 0.0 0.0 0.0 4.224998045938404E-5 0.0 6 0.0 0.0 0.0 4.224998045938404E-5 0.0 7 0.0 0.0 0.0 4.224998045938404E-5 0.0 8 0.0 0.0 0.0 4.224998045938404E-5 0.0 9 0.0 0.0 0.0 1.267499413781521E-4 0.0 10 0.0 0.0 0.0 1.267499413781521E-4 0.0 11 0.0 0.0 0.0 1.267499413781521E-4 0.0 12 0.0 0.0 0.0 1.267499413781521E-4 0.0 13 0.0 0.0 0.0 1.267499413781521E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGTG 80 3.702693E-4 10.709042 7 CTAGGAC 215 0.0 10.606009 3 ATCGCGC 100 2.3470771E-5 10.471064 8 GTCCTAA 205 3.6379788E-12 10.207014 1 ATACCGT 75 0.0026045672 10.153758 6 CCGGATG 85 6.702852E-4 10.041197 19 TGTCCCG 95 1.6640441E-4 9.988384 11 TAGGACT 260 0.0 9.88527 4 GTCTTGC 415 0.0 9.854856 1 TAGACAG 405 0.0 9.636669 5 TACACTG 555 0.0 9.604907 5 GGTATCA 5160 0.0 9.58481 1 TATTCCG 100 2.7010147E-4 9.519149 5 TCTTAGG 250 0.0 9.501217 2 CTTAGCC 190 1.0495569E-9 9.501217 3 TAGACTG 365 0.0 9.38875 5 GTCTTAC 215 1.03682396E-10 9.289893 1 TGTTAGG 260 0.0 9.135786 2 AGTACCA 240 1.0913936E-11 9.122518 5 TAACACG 115 1.0868357E-4 9.105272 4 >>END_MODULE