FastQCFastQC Report
Wed 25 May 2016
SRR1294653_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294653_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158662
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT160021.3810757580726734No Hit
GTACATGGGAAGCAGTGGTATCAAC106580.9198541075827118No Hit
GGTATCAACGCAGAGTACTTTTTTT105520.9107056242458974No Hit
CCCATGTACTCTGCGTTGATACCAC105510.9106193177993237No Hit
TATCAACGCAGAGTACTTTTTTTTT92170.7954865180699808No Hit
GTACTGGTTCACTATCGGTCAGTCA54590.4711468918459395No Hit
GAGTACATGGGAAGCAGTGGTATCA49900.4306691684028647No Hit
CATGTACTCTGCGTTGATACCACTG41830.36101986601787234No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37180.3208873683610924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34730.2997422889505309No Hit
GTTAATGATAGTGTGTCGAAACACA33620.29016227338084793No Hit
GCGTTGATACCACTGCTTCCCATGT32460.28015072557829634No Hit
GTATCAACGCAGAGTACATGGGAAG30570.26383880717586317No Hit
ATACAGGGTGACAGCCCCGTACACA30440.2627168233704048No Hit
GGATACCACGTGTCCCGCCCTACTC29860.25771104946912904No Hit
GGTATCAACGCAGAGTACATGGGAA29380.25356834003359047No Hit
ACGCAGAGTACATGGGAAGCAGTGG28750.2481310338994461No Hit
TATCAACGCAGAGTACATGGGAAGC28080.2423485019790068No Hit
GGTATCAACGCAGAGTACATGGGGG25370.21895945495752858No Hit
GTGGTATCAACGCAGAGTACATGGG25360.21887314851095485No Hit
ACTCTGCGTTGATACCACTGCTTCC24320.20989727806728795No Hit
ACTTAGATGTTTCAGTTCCCCCGGT22440.19367166611142853No Hit
CTCTACTTGTGCGCTATCGGTCTCT20950.18081200557194418No Hit
GTTCACTATCGGTCAGTCAGGAGTA19920.17192244157485098No Hit
GTATCAACGCAGAGTACATGGGGGT19760.17054153842967149No Hit
ATTCCATTCCATTCCATTCCATTCC19590.16907432883791823No Hit
GTACATGGGGGTTAAGCGACTAAGC19450.16786603858588614No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18790.16216981311202058No Hit
GTATTTAGCCTTGGAGGATGGTCCC18660.16104782930656222No Hit
TCATTAACCTATGGATTCAGTTAAT18520.15983953905453013No Hit
AGCGTACACGGTGGATGCCCTGGCA18490.15958061971480897No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18250.15750926499703968No Hit
GTACTTAGATGTTTCAGTTCCCCCG17750.15319394266835368No Hit
GTGGATGCCCTGGCAGTCAGAGGCG16790.1449085237972765No Hit
GAATGGAATGGAATGGAATGGAATG15900.1372272500522154No Hit
CCCATATTCAGACAGGATACCACGT15760.1360189598001833No Hit
CTATCGGTCAGTCAGGAGTATTTAG15680.13532850822759354No Hit
GCTTCCCATGTACTCTGCGTTGATA15340.13239408904408706No Hit
GTCAGGAGTATTTAGCCTTGGAGGA15010.12954597630715428No Hit
GCCTTGGAGGATGGTCCCCCCATAT14990.12937336341400685No Hit
TATTCAGACAGGATACCACGTGTCC14610.12609371844420547No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG14080.1215194767757983No Hit
ATCATTAACTGAATCCATAGGTTAA13980.12065641231006109No Hit
ATACCACTGCTTCCCATGTACTCTG13970.12057010586348736No Hit
GCCCAGAGCCTGAATCAGTGTGTGT13920.12013857363061876No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13880.11979334784432388No Hit
ATCAACGCAGAGTACTTTTTTTTTT13740.1185850575922918No Hit
GCAGAGTACTTTTTTTTTTTTTTTT13340.11513279972934298No Hit
GGGTACTTAGATGTTTCAGTTCCCC13280.11461496104990065No Hit
CAGTGGTATCAACGCAGAGTACATG13170.11366559013758974No Hit
GGTATCAACGCAGAGTACATGGGGA13100.11306144501157368No Hit
CCTATGGATTCAGTTAATGATAGTG13050.1126299127787051No Hit
GTACTCTGCGTTGATACCACTGCTT13010.11228468699241022No Hit
CATGGGAAGCAGTGGTATCAACGCA12240.10563909060623375No Hit
GGGAAGCAGTGGTATCAACGCAGAG12060.10408557456790678No Hit
GGTTCACTATCGGTCAGTCAGGAGT11930.10296359076244842No Hit
GTGCTAATCTGCGATAAGCGTCGGT11860.10235944563643237No Hit
TCACGGTACTGGTTCACTATCGGTC11830.10210052629671121No Hit
GGTTAATGAGGCGAACCGGGGGAAC11750.10141007472412145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCA350.00217334416.281689
AGACACG400.00528822514.24585311
GTCTAGA500.001497551913.3018751
GTACAAA3450.012.9438531
CTATACC604.0991852E-412.66352754
TACATGA2500.012.5368932
CTAGAGC550.003073331412.08791353
ACAGGTG550.003073331412.08791355
TACACTA550.003073331412.08791355
ATACTGT1103.8251528E-812.0873916
TCTACTT2950.011.9123022
GTACTAG1052.712386E-711.7635621
GTATTAC658.011225E-411.6939551
CTGGGTC658.0374005E-411.689414
CACGTCC907.480039E-611.6077337
ACCCCGT1157.026392E-811.56984714
GCCCAAA752.0702304E-411.40160751
CCTCAGA752.0702304E-411.40160751
ACGCTTC2000.011.3966837
CGCTTCC2000.011.3966838
Produced by FastQC (version 0.11.5)