FastQCFastQC Report
Wed 25 May 2016
SRR1294653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158662
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT452103.9019144495978986No Hit
GGTATCAACGCAGAGTACTTTTTTT316982.7357417434937887No Hit
TATCAACGCAGAGTACTTTTTTTTT289282.4966728864845833No Hit
ACGCAGAGTACTTTTTTTTTTTTTT161911.3973876764751065No Hit
CCCATGTACTCTGCGTTGATACCAC119071.0276508593532887No Hit
GTACTTTTTTTTTTTTTTTTTTTTT105100.9070807534898011No Hit
GTACATGGGAAGCAGTGGTATCAAC101460.875665206936967No Hit
GAGTACTTTTTTTTTTTTTTTTTTT82550.7124597164660618No Hit
GCAGAGTACTTTTTTTTTTTTTTTT60550.5225855340038769No Hit
GAGTACATGGGAAGCAGTGGTATCA51450.4440466676217914No Hit
GTACTGGTTCACTATCGGTCAGTCA50750.4380052163616309No Hit
ATCAACGCAGAGTACTTTTTTTTTT48230.41625599182505335No Hit
CATGTACTCTGCGTTGATACCACTG47080.4063307504690756No Hit
ACGCAGAGTACATGGGAAGCAGTGG35810.30906338518049264No Hit
GCGTTGATACCACTGCTTCCCATGT35530.30664680467642846No Hit
GTTAATGATAGTGTGTCGAAACACA34650.2990518373779411No Hit
ATACAGGGTGACAGCCCCGTACACA33980.29326930545750185No Hit
GTGGTATCAACGCAGAGTACTTTTT31450.2714337744743506No Hit
GGTATCAACGCAGAGTACATGGGAA31200.26927611331000756No Hit
TATCAACGCAGAGTACATGGGAAGC30980.26737737148538576No Hit
GTATCAACGCAGAGTACATGGGAAG30120.2599550170800458No Hit
GGATACCACGTGTCCCGCCCTACTC29950.25848780748829253No Hit
ACTCTGCGTTGATACCACTGCTTCC28460.2456281469488082No Hit
GTGGTATCAACGCAGAGTACATGGG27260.23527137335996173No Hit
GGTATCAACGCAGAGTACATGGGGG26640.22992037367239107No Hit
ACTTAGATGTTTCAGTTCCCCCGGT25800.22267063216019856No Hit
CTCTACTTGTGCGCTATCGGTCTCT22730.1961745530620664No Hit
ACCCTGTATCGCGCGCCTTTCCAGA22560.19470734347031315No Hit
AGCGTACACGGTGGATGCCCTGGCA21500.18555886013349882No Hit
CAACGCAGAGTACTTTTTTTTTTTT19650.16959216751736053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA19640.1695058610707868No Hit
ATTCCATTCCATTCCATTCCATTCC19340.1669166676735752No Hit
GTATCAACGCAGAGTACATGGGGGT19140.1651905387421008No Hit
GTTCACTATCGGTCAGTCAGGAGTA18190.15699142631759735No Hit
TCATTAACCTATGGATTCAGTTAAT18150.1566462005313025No Hit
CTATCGGTCAGTCAGGAGTATTTAG17770.1533665555615011No Hit
GCTTCCCATGTACTCTGCGTTGATA17660.15241718464919018No Hit
CCCATATTCAGACAGGATACCACGT17450.15060474927114206No Hit
GTACATGGGGGTTAAGCGACTAAGC17430.1504321363779946No Hit
GTCAGGAGTATTTAGCCTTGGAGGA16740.1444769915644079No Hit
AACGCAGAGTACTTTTTTTTTTTTT16710.14421807222468674No Hit
GAATGGAATGGAATGGAATGGAATG16660.14378653999181815No Hit
GTGGATGCCCTGGCAGTCAGAGGCG16610.14335500775894955No Hit
GTACTTAGATGTTTCAGTTCCCCCG16290.1405932014685905No Hit
GTATTTAGCCTTGGAGGATGGTCCC15790.1362778791399045No Hit
CAGTGGTATCAACGCAGAGTACATG15370.13265300838380822No Hit
GCCTTGGAGGATGGTCCCCCCATAT15080.1301501214331703No Hit
CCTATGGATTCAGTTAATGATAGTG14910.12868291184141709No Hit
CATGGGAAGCAGTGGTATCAACGCA14850.12816507316197476No Hit
ATACCACTGCTTCCCATGTACTCTG14790.12764723448253243No Hit
TCAACGCAGAGTACTTTTTTTTTTT14700.12687047646336896No Hit
ACATGGGAAGCAGTGGTATCAACGC14520.12531696042504198No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG14170.12229623479496177No Hit
GCCCAGAGCCTGAATCAGTGTGTGT14070.12143317032922457No Hit
GGTATCAACGCAGAGTACATGGGGA13990.12074271875663481No Hit
TATTCAGACAGGATACCACGTGTCC13840.119448122058029No Hit
ATCATTAACTGAATCCATAGGTTAA13700.11823983180599691No Hit
GTACTCTGCGTTGATACCACTGCTT13690.11815352535942321No Hit
GGGTACTTAGATGTTTCAGTTCCCC13520.11668631576766995No Hit
GGGAAGCAGTGGTATCAACGCAGAG13460.11616847708822763No Hit
CGGTACTGGTTCACTATCGGTCAGT13210.11401081592388462No Hit
TCACGGTACTGGTTCACTATCGGTC13110.11314775145814741No Hit
ACGCAGAGTACATGGGGGTTAAGCG12860.1109900902938044No Hit
GATACCACTGCTTCCCATGTACTCT12410.107106300197987No Hit
GCAGAGTACATGGGAAGCAGTGGTA12110.10451710680077539No Hit
GGTTAATGAGGCGAACCGGGGGAAC12100.10443080035420166No Hit
ATGATAGTGTGTCGAAACACACTGG11990.10348142944189072No Hit
GGTTCACTATCGGTCAGTCAGGAGT11700.10097854249125283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG603.610694E-919.0440735
GACGGCT250.005950983619.0440737
TTACGGC350.002197770916.2544618
GTACAAA1550.014.7227431
GACCGTT400.00519750314.2830547
GTGAACC903.7261998E-813.69647819
CTAGAAG500.001500840813.2980023
AGGACCG655.332913E-513.184365
TTGTGCG2950.012.9112367
TGTGCGC2950.012.9112368
GCGCTAT3050.012.75423811
CAACCCC1207.6215656E-1012.64290112
TGCGCTA3100.012.284368510
TCTACTT3100.012.2562242
ACTGGCC701.0670989E-412.24261958
CCGAACA701.1101671E-412.19084517
ATAGTGC550.003014847912.1189568
TAGGAGT550.003014847912.1189564
TAGCACC657.866276E-411.7194314
GTGCGCT3250.011.71749
Produced by FastQC (version 0.11.5)