##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294653_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1158662 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89355825944063 31.0 31.0 33.0 28.0 34.0 2 30.914315822906076 31.0 31.0 34.0 27.0 34.0 3 31.07125546535573 31.0 31.0 34.0 27.0 34.0 4 34.530966753030654 37.0 35.0 37.0 31.0 37.0 5 34.94394051069251 37.0 35.0 37.0 32.0 37.0 6 34.30370461791273 37.0 35.0 37.0 30.0 37.0 7 34.17228579171493 37.0 35.0 37.0 30.0 37.0 8 34.20325168168111 37.0 35.0 37.0 30.0 37.0 9 35.398458739477086 39.0 35.0 39.0 28.0 39.0 10 35.310906027814845 39.0 35.0 39.0 28.0 39.0 11 35.421348072172904 39.0 35.0 39.0 28.0 39.0 12 35.44040626170531 39.0 35.0 39.0 28.0 39.0 13 35.15085849022407 39.0 35.0 39.0 27.0 39.0 14 36.05183651487664 39.0 36.0 41.0 27.0 41.0 15 36.02786058401846 39.0 36.0 41.0 27.0 41.0 16 36.10216525613164 39.0 36.0 41.0 27.0 41.0 17 36.36368069376574 39.0 36.0 41.0 27.0 41.0 18 36.4637823627598 39.0 36.0 41.0 28.0 41.0 19 36.5264641457129 39.0 36.0 41.0 28.0 41.0 20 36.65516172965023 39.0 36.0 41.0 28.0 41.0 21 36.50439127200167 39.0 36.0 41.0 27.0 41.0 22 36.56201118186322 39.0 36.0 41.0 28.0 41.0 23 36.56879659469284 39.0 35.0 41.0 28.0 41.0 24 36.48115153513277 39.0 35.0 41.0 28.0 41.0 25 35.36769739578928 38.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.41568705730387734 1101 2 0.31578553070076865 1101 3 0.35846173643069434 1101 4 0.18338387912704945 1101 5 0.2235575118804931 1101 6 0.282055286248287 1101 7 0.2789278381431828 1101 8 0.2597649778885227 1101 9 0.5228212722596837 1101 10 0.3217059660086363 1101 11 0.39556995093091984 1101 12 0.39331524754749836 1101 13 0.40051610254959513 1101 14 0.6211830907496108 1101 15 0.4826568258441384 1101 16 0.6483713822962969 1101 17 0.5548809907347518 1101 18 0.4667508152381501 1101 19 0.7100406400486037 1101 20 0.6260505088015762 1101 21 0.9930518264960924 1101 22 0.8543482574999359 1101 23 0.7160576881524676 1101 24 0.9464266736966209 1101 25 0.8879773623349934 1102 1 0.44961644749186647 1102 2 0.4166566907191118 1102 3 0.2928954824187713 1102 4 0.32873005060802285 1102 5 0.2840802078914919 1102 6 0.4095197520896434 1102 7 0.45499386290246235 1102 8 0.49222715120351523 1102 9 0.5874705157259825 1102 10 0.80680454464229 1102 11 0.7408244213573312 1102 12 0.8715362470889971 1102 13 0.765944804979668 1102 14 0.982943750997201 1102 15 1.0305706268528496 1102 16 0.9450242938047779 1102 17 0.6970176253060458 1102 18 0.7152606731015183 1102 19 0.9149924969949623 1102 20 0.8021165335608558 1102 21 1.0136845637725713 1102 22 1.0372918613513775 1102 23 1.0127105996609487 1102 24 0.9977792642514132 1102 25 1.024153428359753 1103 1 0.41871364791626675 1103 2 0.4023330108589711 1103 3 0.37474934375625324 1103 4 0.2752776177848446 1103 5 0.30629564818605104 1103 6 0.28006193820051806 1103 7 0.24081906776398654 1103 8 0.3282666601449904 1103 9 0.4373938461027578 1103 10 0.5390588056024441 1103 11 0.5510088324681206 1103 12 0.6404715514355956 1103 13 0.695104393613633 1103 14 0.7309585350470158 1103 15 0.7162475478355859 1103 16 0.9123577095986164 1103 17 0.7676050611013281 1103 18 0.8285738388328383 1103 19 0.907004808887109 1103 20 0.8190283959189415 1103 21 1.0770125744667496 1103 22 0.8504054850907252 1103 23 1.0444191502559619 1103 24 1.1602602315172845 1103 25 1.0991210087426921 1104 1 0.22748904540769388 1104 2 0.23059150811236862 1104 3 0.13319341727359557 1104 4 0.13532067401924053 1104 5 0.21372740835811044 1104 6 0.28225553140824644 1104 7 0.18108156529199704 1104 8 0.2436405638863306 1104 9 0.10477675046197277 1104 10 0.42601497984801284 1104 11 0.5930709412399295 1104 12 0.6334366422768767 1104 13 0.12543352481423398 1104 14 0.3955367386736981 1104 15 0.6070115654474506 1104 16 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-0.08702574173682365 2206 24 0.31742972084122556 2206 25 0.25748394271689534 2207 1 0.13350173263202336 2207 2 0.018731451456783788 2207 3 0.21093888381410508 2207 4 0.2479221530058453 2207 5 0.11975745684425476 2207 6 0.33517207871624066 2207 7 0.30438375445065446 2207 8 0.28620343335568776 2207 9 0.2708719422605128 2207 10 0.5045046658117229 2207 11 0.5281951851810902 2207 12 0.3974181969007091 2207 13 0.46260485442647337 2207 14 0.35864347860488976 2207 15 0.28289031053686386 2207 16 0.559716238134321 2207 17 0.3718328531418251 2207 18 0.5256278849233453 2207 19 0.5273509595766583 2207 20 0.31900312088719573 2207 21 0.784854157871564 2207 22 0.6442340719396711 2207 23 0.5825090075106516 2207 24 0.95451263163622 2207 25 0.8449418350997604 2208 1 0.08497786514227101 2208 2 0.026390097135170265 2208 3 0.1271483370679647 2208 4 0.1595665195071092 2208 5 0.08431734048372874 2208 6 0.1139532315498002 2208 7 0.07949388163667237 2208 8 0.059716075809717495 2208 9 0.20807266023278714 2208 10 0.30164954707001357 2208 11 -0.0053969660015980025 2208 12 -0.27689218456416853 2208 13 -0.08306500517905846 2208 14 -0.4837416112509345 2208 15 0.12338318008600169 2208 16 0.05042140688252772 2208 17 0.044618313952383915 2208 18 0.181313128113338 2208 19 0.531480841033833 2208 20 0.436136234222964 2208 21 0.6663799528782235 2208 22 0.7028371794022306 2208 23 0.650663518469976 2208 24 0.7505446845331036 2208 25 0.7306130994024969 2209 1 -0.10058180557420826 2209 2 -0.10067105980788327 2209 3 -0.12774013853866606 2209 4 -0.6658088343878958 2209 5 -0.3014910060516769 2209 6 -0.06794341609528232 2209 7 0.06451116354639197 2209 8 0.0114581311648152 2209 9 -0.08187451381732558 2209 10 0.005358667008408702 2209 11 0.028342408317328704 2209 12 0.06164868180966465 2209 13 0.0855327846439522 2209 14 -0.04534804291188976 2209 15 -0.6002703410258334 2209 16 -0.596372388231913 2209 17 -0.07170827793492407 2209 18 -0.361633256461694 2209 19 0.25050012577294467 2209 20 0.23056116256088188 2209 21 0.5165875653960867 2209 22 0.35817461376718285 2209 23 0.3471824102377141 2209 24 0.4515051242371655 2209 25 0.39816108164902175 2210 1 -0.12355540993312886 2210 2 -0.14739292509753454 2210 3 -0.0787099356168568 2210 4 0.02810576927799957 2210 5 -0.079956074424679 2210 6 -0.6286068417100168 2210 7 -0.13257779516580115 2210 8 -0.42174840860407414 2210 9 0.045801047504731685 2210 10 0.22741779449499688 2210 11 0.27153819668670565 2210 12 0.08651064182974721 2210 13 0.20266328995973026 2210 14 0.23026555977961038 2210 15 0.11718652745103242 2210 16 0.2232342390766462 2210 17 -0.7727559748948281 2210 18 -1.3129658915619729 2210 19 -0.18206740916910746 2210 20 -0.07300972633147751 2210 21 -0.26813815233364835 2210 22 -0.3772565451433678 2210 23 -0.2774865803986657 2210 24 0.23463845374932646 2210 25 0.2734344259942887 2211 1 -0.3563096334841518 2211 2 -0.2581544250435037 2211 3 -0.09161581076544678 2211 4 -0.15626366986324314 2211 5 -0.06827890141217807 2211 6 -0.030199363593233386 2211 7 -0.1981622481754215 2211 8 -0.16557860204090957 2211 9 -0.04023655920972402 2211 10 0.02371980090686776 2211 11 -0.2795338108058161 2211 12 -0.36140117690877105 2211 13 -0.11436239355311528 2211 14 -0.26232188303585247 2211 15 -0.28667176295637375 2211 16 -0.20271547455038785 2211 17 -0.4941374092811941 2211 18 -0.42668657885710104 2211 19 -0.1794169282786484 2211 20 -0.290387702629161 2211 21 0.12712534681983811 2211 22 -0.14560170562029384 2211 23 -0.71928190477459 2211 24 -0.1650485171811198 2211 25 -0.9492502755654542 2212 1 0.02980020633278002 2212 2 -0.07588897881924694 2212 3 -0.011615810765444934 2212 4 0.27865623860358113 2212 5 0.24982178508668085 2212 6 0.22968621993079807 2212 7 0.25110548637835706 2212 8 0.10968455585382486 2212 9 0.4081158435362724 2212 10 0.0157564141791795 2212 11 0.28103827157404737 2212 12 0.11356449814843472 2212 13 0.17156437990226436 2212 14 0.308250199344009 2212 15 0.3009026077530095 2212 16 0.12801679089584184 2212 17 -0.04038454886929799 2212 18 0.22297017171498368 2212 19 0.039210074009680795 2212 20 -0.02880875526074078 2212 21 0.42719399670541947 2212 22 0.33108021657650255 2212 23 0.3032810242871946 2212 24 -0.0536755194694436 2212 25 0.3563561317572024 2213 1 -0.12907400175209816 2213 2 -0.09798853149841236 2213 3 0.013728162817383094 2213 4 0.13504225041351248 2213 5 -0.0692758351333822 2213 6 -0.05555984790803592 2213 7 -0.21514392912518332 2213 8 -0.08106881353552353 2213 9 -0.057713562118756556 2213 10 -0.1359672814109203 2213 11 0.0026220190550247935 2213 12 0.2517107029559966 2213 13 0.26601735328232934 2213 14 -0.25511770031708636 2213 15 0.02310416282395522 2213 16 -0.03745798575409509 2213 17 0.16142356249331158 2213 18 0.12661949866471645 2213 19 -0.0191307422577367 2213 20 -0.08720584870982862 2213 21 0.23498445524030842 2213 22 -0.22403790343458496 2213 23 -0.09592550883151318 2213 24 -0.49547229263516357 2213 25 -0.1091252657376316 2214 1 -0.4568398180321651 2214 2 -0.3918109780619581 2214 3 -0.2865063331301627 2214 4 -0.2183343611363071 2214 5 -0.15979907657639103 2214 6 -0.2633961105434963 2214 7 -0.5179526767021656 2214 8 -0.14792455136840488 2214 9 -0.4569616139485433 2214 10 -0.4066539870193111 2214 11 -0.18894263157528712 2214 12 -0.5212510361518099 2214 13 -0.6086069978696571 2214 14 -0.4299196309244948 2214 15 -0.2106567488806803 2214 16 -0.5278781270370914 2214 17 -0.6026250539480742 2214 18 -0.5167710330328887 2214 19 -0.5728826774184697 2214 20 -0.4415419096982873 2214 21 -0.5807085599677748 2214 22 -0.603121255198019 2214 23 -1.004202142497455 2214 24 -0.6462668468651955 2214 25 -0.9930335098475922 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 11.0 10 48.0 11 96.0 12 311.0 13 870.0 14 1865.0 15 3600.0 16 5794.0 17 7992.0 18 9218.0 19 9492.0 20 9210.0 21 9006.0 22 8708.0 23 8612.0 24 8899.0 25 9512.0 26 10160.0 27 11078.0 28 12767.0 29 15567.0 30 19733.0 31 25850.0 32 34737.0 33 47351.0 34 65210.0 35 91139.0 36 130518.0 37 209114.0 38 392194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.43244037077671 21.13816011126757 12.531509973478927 19.89788954447679 2 15.148075970386532 24.001736485705063 37.717039136521265 23.133148407387143 3 18.76628387586275 32.19711666838057 29.532329245603407 19.504270210153273 4 11.336752626618008 20.060611605801864 34.81412555976397 33.78851020781615 5 11.948467206718187 40.028038480397235 30.790533214549626 17.232961098334957 6 27.73934765270292 38.638388155963966 16.436085916217532 17.186178275115576 7 21.661587866126848 39.19467200732038 21.58044215778524 17.563297968767536 8 25.619595720859728 34.9587425342503 20.482244917161363 18.939416827728607 9 26.45018689677136 15.959974487792374 21.528902759305783 36.060935856130484 10 18.92847736283235 30.629228007430203 27.146493526296823 23.295801103440628 11 30.06096344525078 29.03506067413013 19.075797969957865 21.82817791066122 12 22.774110192128934 31.70114504047386 28.82538197504162 16.69936279235558 13 29.549946403696676 25.18499786823077 26.384657475605483 18.88039825246707 14 22.430021887579315 25.59042953511334 29.40366703261042 22.575881544696923 15 27.17753795803385 26.157987585530723 27.752345837562398 18.91212861887303 16 18.702089133845764 30.73476130226071 29.25564142088029 21.307508143013234 17 18.664820857869557 28.981902385598552 30.284095175379168 22.069181581152726 18 18.870381829998006 24.290795291445182 35.36732613707076 21.47149674148605 19 19.411319465002215 25.494990329337625 37.910291121028706 17.183399084631457 20 21.62810628493802 21.809954438708886 37.05473310093824 19.507206175414854 21 21.2846920448553 24.65980068337621 36.55268720132498 17.502820070443505 22 20.438743065073517 24.06458328298643 35.66502794684748 19.831645705092573 23 19.322028339584797 24.743454087559616 37.47417279586281 18.460344776992773 24 19.70669617196387 24.32892422466604 36.182251597100795 19.7821280062693 25 20.280553788632677 23.375224509488305 36.02465655766215 20.31956514421687 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2593.0 1 2593.0 2 3104.0 3 3615.0 4 3615.0 5 3615.0 6 8758.5 7 13902.0 8 13902.0 9 13902.0 10 13536.5 11 13171.0 12 13171.0 13 13171.0 14 10783.5 15 8396.0 16 8396.0 17 8396.0 18 14458.0 19 20520.0 20 20520.0 21 20520.0 22 31981.5 23 43443.0 24 43443.0 25 43443.0 26 61734.5 27 80026.0 28 80026.0 29 80026.0 30 93047.5 31 106069.0 32 106069.0 33 106069.0 34 111774.0 35 117479.0 36 117479.0 37 117479.0 38 108743.5 39 100008.0 40 100008.0 41 100008.0 42 104053.0 43 108098.0 44 108098.0 45 108098.0 46 141500.0 47 174902.0 48 174902.0 49 174902.0 50 160404.0 51 145906.0 52 145906.0 53 145906.0 54 117896.5 55 89887.0 56 89887.0 57 89887.0 58 71351.0 59 52815.0 60 52815.0 61 52815.0 62 47351.5 63 41888.0 64 41888.0 65 41888.0 66 30303.5 67 18719.0 68 18719.0 69 18719.0 70 14628.5 71 10538.0 72 10538.0 73 10538.0 74 7452.0 75 4366.0 76 4366.0 77 4366.0 78 2972.5 79 1579.0 80 1579.0 81 1579.0 82 1035.0 83 491.0 84 491.0 85 491.0 86 306.5 87 122.0 88 122.0 89 122.0 90 103.5 91 85.0 92 85.0 93 85.0 94 58.0 95 31.0 96 31.0 97 31.0 98 22.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09424663965850265 2 0.0 3 0.12592110555105804 4 0.011910289627173411 5 0.021921837429724977 6 0.011910289627173411 7 0.021749224536577533 8 0.017692821547612675 9 8.630644657372038E-5 10 0.38440891303935054 11 0.06516136716315889 12 0.001121983805458365 13 0.0 14 0.0012082902520320854 15 0.0012082902520320854 16 0.0 17 8.630644657372038E-5 18 4.3153223286860194E-4 19 2.589193397211612E-4 20 2.589193397211612E-4 21 2.589193397211612E-4 22 0.0029344191835064927 23 0.0 24 0.0 25 0.0021576611643430096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1158662.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.834396561794854 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84152854520804 32.202735067314755 2 11.701545055642544 9.322479722643498 3 3.349380728280984 4.002616805003338 4 1.3682828325781677 2.1801888384645873 5 0.6680635925328393 1.3305955036725226 6 0.4071147486451602 0.9730302206212047 7 0.2588718604464374 0.7218403043399103 8 0.19608545909141178 0.6248756749959119 9 0.14056378867915204 0.5039346330426305 >10 0.8460454142227486 6.40128601872928 >50 0.09207704375252514 2.5883484576656772 >100 0.09974894718067075 8.583589106642277 >500 0.01381139268270301 3.928028384736823 >1k 0.01446907804854601 10.99420038898271 >5k 8.769138211240006E-4 2.14216399016439 >10k+ 0.0015345991869670011 13.500086882980556 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 45210 3.9019144495978986 No Hit GGTATCAACGCAGAGTACTTTTTTT 31698 2.7357417434937887 No Hit TATCAACGCAGAGTACTTTTTTTTT 28928 2.4966728864845833 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 16191 1.3973876764751065 No Hit CCCATGTACTCTGCGTTGATACCAC 11907 1.0276508593532887 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 10510 0.9070807534898011 No Hit GTACATGGGAAGCAGTGGTATCAAC 10146 0.875665206936967 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 8255 0.7124597164660618 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 6055 0.5225855340038769 No Hit GAGTACATGGGAAGCAGTGGTATCA 5145 0.4440466676217914 No Hit GTACTGGTTCACTATCGGTCAGTCA 5075 0.4380052163616309 No Hit ATCAACGCAGAGTACTTTTTTTTTT 4823 0.41625599182505335 No Hit CATGTACTCTGCGTTGATACCACTG 4708 0.4063307504690756 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3581 0.30906338518049264 No Hit GCGTTGATACCACTGCTTCCCATGT 3553 0.30664680467642846 No Hit GTTAATGATAGTGTGTCGAAACACA 3465 0.2990518373779411 No Hit ATACAGGGTGACAGCCCCGTACACA 3398 0.29326930545750185 No Hit GTGGTATCAACGCAGAGTACTTTTT 3145 0.2714337744743506 No Hit GGTATCAACGCAGAGTACATGGGAA 3120 0.26927611331000756 No Hit TATCAACGCAGAGTACATGGGAAGC 3098 0.26737737148538576 No Hit GTATCAACGCAGAGTACATGGGAAG 3012 0.2599550170800458 No Hit GGATACCACGTGTCCCGCCCTACTC 2995 0.25848780748829253 No Hit ACTCTGCGTTGATACCACTGCTTCC 2846 0.2456281469488082 No Hit GTGGTATCAACGCAGAGTACATGGG 2726 0.23527137335996173 No Hit GGTATCAACGCAGAGTACATGGGGG 2664 0.22992037367239107 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 2580 0.22267063216019856 No Hit CTCTACTTGTGCGCTATCGGTCTCT 2273 0.1961745530620664 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 2256 0.19470734347031315 No Hit AGCGTACACGGTGGATGCCCTGGCA 2150 0.18555886013349882 No Hit CAACGCAGAGTACTTTTTTTTTTTT 1965 0.16959216751736053 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1964 0.1695058610707868 No Hit ATTCCATTCCATTCCATTCCATTCC 1934 0.1669166676735752 No Hit GTATCAACGCAGAGTACATGGGGGT 1914 0.1651905387421008 No Hit GTTCACTATCGGTCAGTCAGGAGTA 1819 0.15699142631759735 No Hit TCATTAACCTATGGATTCAGTTAAT 1815 0.1566462005313025 No Hit CTATCGGTCAGTCAGGAGTATTTAG 1777 0.1533665555615011 No Hit GCTTCCCATGTACTCTGCGTTGATA 1766 0.15241718464919018 No Hit CCCATATTCAGACAGGATACCACGT 1745 0.15060474927114206 No Hit GTACATGGGGGTTAAGCGACTAAGC 1743 0.1504321363779946 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 1674 0.1444769915644079 No Hit AACGCAGAGTACTTTTTTTTTTTTT 1671 0.14421807222468674 No Hit GAATGGAATGGAATGGAATGGAATG 1666 0.14378653999181815 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 1661 0.14335500775894955 No Hit GTACTTAGATGTTTCAGTTCCCCCG 1629 0.1405932014685905 No Hit GTATTTAGCCTTGGAGGATGGTCCC 1579 0.1362778791399045 No Hit CAGTGGTATCAACGCAGAGTACATG 1537 0.13265300838380822 No Hit GCCTTGGAGGATGGTCCCCCCATAT 1508 0.1301501214331703 No Hit CCTATGGATTCAGTTAATGATAGTG 1491 0.12868291184141709 No Hit CATGGGAAGCAGTGGTATCAACGCA 1485 0.12816507316197476 No Hit ATACCACTGCTTCCCATGTACTCTG 1479 0.12764723448253243 No Hit TCAACGCAGAGTACTTTTTTTTTTT 1470 0.12687047646336896 No Hit ACATGGGAAGCAGTGGTATCAACGC 1452 0.12531696042504198 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 1417 0.12229623479496177 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 1407 0.12143317032922457 No Hit GGTATCAACGCAGAGTACATGGGGA 1399 0.12074271875663481 No Hit TATTCAGACAGGATACCACGTGTCC 1384 0.119448122058029 No Hit ATCATTAACTGAATCCATAGGTTAA 1370 0.11823983180599691 No Hit GTACTCTGCGTTGATACCACTGCTT 1369 0.11815352535942321 No Hit GGGTACTTAGATGTTTCAGTTCCCC 1352 0.11668631576766995 No Hit GGGAAGCAGTGGTATCAACGCAGAG 1346 0.11616847708822763 No Hit CGGTACTGGTTCACTATCGGTCAGT 1321 0.11401081592388462 No Hit TCACGGTACTGGTTCACTATCGGTC 1311 0.11314775145814741 No Hit ACGCAGAGTACATGGGGGTTAAGCG 1286 0.1109900902938044 No Hit GATACCACTGCTTCCCATGTACTCT 1241 0.107106300197987 No Hit GCAGAGTACATGGGAAGCAGTGGTA 1211 0.10451710680077539 No Hit GGTTAATGAGGCGAACCGGGGGAAC 1210 0.10443080035420166 No Hit ATGATAGTGTGTCGAAACACACTGG 1199 0.10348142944189072 No Hit GGTTCACTATCGGTCAGTCAGGAGT 1170 0.10097854249125283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCCG 60 3.610694E-9 19.044073 5 GACGGCT 25 0.0059509836 19.044073 7 TTACGGC 35 0.0021977709 16.25446 18 GTACAAA 155 0.0 14.722743 1 GACCGTT 40 0.005197503 14.283054 7 GTGAACC 90 3.7261998E-8 13.696478 19 CTAGAAG 50 0.0015008408 13.298002 3 AGGACCG 65 5.332913E-5 13.18436 5 TTGTGCG 295 0.0 12.911236 7 TGTGCGC 295 0.0 12.911236 8 GCGCTAT 305 0.0 12.754238 11 CAACCCC 120 7.6215656E-10 12.642901 12 TGCGCTA 310 0.0 12.2843685 10 TCTACTT 310 0.0 12.256224 2 ACTGGCC 70 1.0670989E-4 12.2426195 8 CCGAACA 70 1.1101671E-4 12.190845 17 ATAGTGC 55 0.0030148479 12.118956 8 TAGGAGT 55 0.0030148479 12.118956 4 TAGCACC 65 7.866276E-4 11.719431 4 GTGCGCT 325 0.0 11.7174 9 >>END_MODULE