FastQCFastQC Report
Wed 25 May 2016
SRR1294652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589848
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88271.4964872306085635No Hit
GGTATCAACGCAGAGTACTTTTTTT58750.9960193134502449No Hit
GTACTGGTTCACTATCGGTCAGTCA52900.8968412201109438No Hit
TATCAACGCAGAGTACTTTTTTTTT51310.8698851229469288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA37330.6328749101463428No Hit
ATACAGGGTGACAGCCCCGTACACA36810.6240590796272938No Hit
GGATACCACGTGTCCCGCCCTACTC31990.5423431121238014No Hit
ACCCTGTATCGCGCGCCTTTCCAGA23010.39010050046791717No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21400.3628053328993232No Hit
GTTAATGATAGTGTGTCGAAACACA19920.3377141229604915No Hit
GTTCACTATCGGTCAGTCAGGAGTA19190.32533805319336506No Hit
GTATTTAGCCTTGGAGGATGGTCCC17770.30126405446826976No Hit
CCCATATTCAGACAGGATACCACGT15940.270239112449309No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG15830.2683742252241256No Hit
GGTTAATGAGGCGAACCGGGGGAAC15200.2576935074798931No Hit
GCCTTGGAGGATGGTCCCCCCATAT15040.2549809442432627No Hit
GTCAGGAGTATTTAGCCTTGGAGGA14580.24718232493795012No Hit
ACTTAGATGTTTCAGTTCCCCCGGT14550.24667371933108193No Hit
GCCCAGAGCCTGAATCAGTGTGTGT14460.24514790251047727No Hit
GGTATCAACGCAGAGTACATGGGGG14430.24463929690360905No Hit
TCTAAGTACCCCGAGGAAAAGAAAT14370.24362208568987262No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13800.23395857915937665No Hit
CTATCGGTCAGTCAGGAGTATTTAG13390.2270076358655111No Hit
ATCATTAACTGAATCCATAGGTTAA13110.2222606502014078No Hit
TATTCAGACAGGATACCACGTGTCC12770.2164964533235681No Hit
TCACGGTACTGGTTCACTATCGGTC12030.20395084835415225No Hit
CACACACACTGATTCAGGCTCTGGG12000.20344224274728404No Hit
CGGTACTGGTTCACTATCGGTCAGT11950.20259456673583703No Hit
CATCTAAGTACCCCGAGGAAAAGAA11560.1959826938465503No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11030.18699732812521191No Hit
AGCGTACACGGTGGATGCCCTGGCA10930.18530197610231788No Hit
GTACATGGGGGTTAAGCGACTAAGC10570.17919870881989935No Hit
CCCCAGTAGCGGCGAGCGAACGGGG10540.17869010321303114No Hit
GGTTCACTATCGGTCAGTCAGGAGT10400.1763166103809795No Hit
TTCCAGACGCTTCCACTAACACACA10250.17377358234663845No Hit
GTGGATGCCCTGGCAGTCAGAGGCG10090.171061019110008No Hit
GTATCAACGCAGAGTACATGGGGGT9990.16936566708711395No Hit
AGCTCACAGCATGTGCATTTTTGTG9910.16800938546879873No Hit
TCATTAACCTATGGATTCAGTTAAT9820.1664835686481941No Hit
GTACTTAGATGTTTCAGTTCCCCCG9810.1663140334459047No Hit
GTCCCGCCCTACTCATCGAGCTCAC9640.16343193500698486No Hit
GGGTTTCCCCATTCGGAAATCGCCG9330.15817634373601336No Hit
GGGTGACAGCCCCGTACACAAAAAT9020.15292075246504186No Hit
GGAGTATTTAGCCTTGGAGGATGGT8810.1493605132169644No Hit
GTGCTAATCTGCGATAAGCGTCGGT8660.14681748518262333No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8530.14461352755286108No Hit
GCCCTACTCATCGAGCTCACAGCAT8430.14291817552996702No Hit
ACGCAGAGTACATGGGGGTTAAGCG8410.14257910512538824No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG8390.14224003472080943No Hit
GGTATCAACGCAGAGTACATGGGGA8390.14224003472080943No Hit
GGGTACTTAGATGTTTCAGTTCCCC8230.13952747148417896No Hit
GTCCCCCCATATTCAGACAGGATAC8210.13918840107960015No Hit
CCTATGGATTCAGTTAATGATAGTG8030.1361367674383909No Hit
GCCTGAATCAGTGTGTGTGTTAGTG7850.13308513379718165No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA7780.1318983873811558No Hit
ATCAACGCAGAGTACTTTTTTTTTT7740.1312202465719982No Hit
GTGGTATCAACGCAGAGTACATGGG7710.13071164096513No Hit
GAATCAGTGTGTGTGTTAGTGGAAG7580.12850768333536775No Hit
ATGATAGTGTGTCGAAACACACTGG7510.12732093691934193No Hit
GTTATAACCGGCGATTTCCGAATGG7400.1254560496941585No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC7310.12393023287355387No Hit
CTACTGGGGGAATCTCGGTTGATTT7170.12155674004150221No Hit
GTATCGCGCGCCTTTCCAGACGCTT7120.1207090640300552No Hit
AGTCAGGAGTATTTAGCCTTGGAGG7030.11918324720945057No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA6990.11850510640029296No Hit
CACTATCATTAACTGAATCCATAGG6850.11613161356824131No Hit
TCCCCCAGTAGCGGCGAGCGAACGG6700.11358858553390026No Hit
TTTGTGTACGGGGCTGTCACCCTGT6620.11223230391558504No Hit
GTGGTATCAACGCAGAGTACTTTTT6550.11104555749955922No Hit
GGTATCAACGCAGAGTACATGGGCA6050.10256879738508905No Hit
TAAGTACCCCGAGGAAAAGAAATCA5970.1012125157667738No Hit
GATATGAACCGTTATAACCGGCGAT5950.100873445362195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA250.00604080818.9928741
TCTATAC359.961424E-518.9912623
CTATACC350.002174722516.2782254
GGTATCA15150.016.1721481
GTACAAA1200.015.0360241
CCGAATG1650.014.98315118
GTCTATA456.7771156E-414.7709812
AATCGCC1550.014.72289918
ACTTAAC400.005248520514.260385516
TACCCTG400.005290078514.2434457
AGGACCG400.005290078514.2434455
TACACTC400.005290078514.2434455
GTAACAA759.684281E-713.9281061
GAAATCG1750.013.5813216
ATCGCCG1750.013.56748919
AAATCGC1700.013.42153917
GTTTAGA500.001501861313.29501151
CGAATGG1950.012.6629919
CGCAGAA853.9518636E-612.2884642
GGTTATA1251.4097168E-912.1554371
Produced by FastQC (version 0.11.5)