##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294652_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 589848 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.44137811775237 31.0 28.0 33.0 16.0 34.0 2 28.377517597753997 31.0 28.0 33.0 16.0 34.0 3 28.394622004312975 31.0 27.0 34.0 16.0 34.0 4 31.339034802186326 35.0 31.0 37.0 16.0 37.0 5 32.37244679985352 35.0 32.0 37.0 20.0 37.0 6 32.28754187519497 35.0 32.0 37.0 18.0 37.0 7 32.22979988064722 35.0 32.0 37.0 17.0 37.0 8 31.998263959528558 35.0 32.0 37.0 17.0 37.0 9 32.4860930273562 37.0 31.0 39.0 13.0 39.0 10 32.81654256689859 37.0 31.0 39.0 15.0 39.0 11 33.038067434322066 37.0 32.0 39.0 16.0 39.0 12 32.956946535378606 37.0 31.0 39.0 16.0 39.0 13 32.66926733666978 37.0 31.0 39.0 13.0 39.0 14 33.29731557960695 38.0 31.0 40.0 13.0 41.0 15 33.35146173251414 38.0 31.0 40.0 13.0 41.0 16 33.54813612998603 38.0 31.0 40.0 16.0 41.0 17 33.69861896624215 38.0 31.0 40.0 17.0 41.0 18 33.669545374401544 38.0 31.0 40.0 17.0 41.0 19 34.00661695894536 38.0 31.0 40.0 18.0 41.0 20 34.14786182202872 38.0 32.0 40.0 18.0 41.0 21 34.23994317180019 38.0 32.0 41.0 18.0 41.0 22 34.364592233931454 38.0 32.0 40.0 19.0 41.0 23 34.43740760331475 38.0 32.0 40.0 21.0 41.0 24 34.54061893911652 38.0 31.0 40.0 23.0 41.0 25 33.50156141921308 37.0 30.0 40.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.038067202214041 1101 2 1.0674024675152864 1101 3 1.4209296720762161 1101 4 1.2230545025778525 1101 5 0.8524838769275576 1101 6 0.7884464502659938 1101 7 0.7240446255546615 1101 8 0.7115324718195204 1101 9 0.9292308157850044 1101 10 0.7160870488019881 1101 11 0.836083970392643 1101 12 1.0734004087464513 1101 13 1.0308527502923326 1101 14 1.1107001909204683 1101 15 1.0091430885274804 1101 16 0.9056350786660019 1101 17 1.0378374019073178 1101 18 1.133339035666772 1101 19 1.277135227505184 1101 20 0.8543923535414493 1101 21 0.7889912377709862 1101 22 0.785046275954933 1101 23 0.5901179819404234 1101 24 1.1423910642500772 1101 25 0.7506736029974448 1102 1 0.8007337334120486 1102 2 0.8359855923538895 1102 3 0.8841545881491477 1102 4 1.130151790241321 1102 5 0.8209772537801925 1102 6 0.6954963238991425 1102 7 0.6481364563712404 1102 8 0.4498095644927851 1102 9 0.7642799443405295 1102 10 0.8010758514707206 1102 11 0.6511201447577406 1102 12 0.9666849733628808 1102 13 0.7680342401935647 1102 14 1.1841140434374324 1102 15 1.1826394922579482 1102 16 1.0735730340206118 1102 17 1.101842109443183 1102 18 1.18370712941109 1102 19 1.0746468375925176 1102 20 0.07204815308170254 1102 21 0.6483612238431178 1102 22 0.46938821578144996 1102 23 0.7665108749159515 1102 24 0.687693900201765 1102 25 0.6091435691726161 1103 1 0.6306979869222893 1103 2 0.4084277860934229 1103 3 0.9380239041352354 1103 4 0.5567273731345281 1103 5 0.4331302551971703 1103 6 0.36402492109094453 1103 7 0.4064306081167004 1103 8 0.3110294537108693 1103 9 0.420826221552943 1103 10 0.32596716281942406 1103 11 0.36027494960123363 1103 12 0.8279233799067427 1103 13 0.6222972506276747 1103 14 0.4149590775737195 1103 15 0.6484371864483407 1103 16 0.7552368559966496 1103 17 0.5101378713908886 1103 18 0.5401004374105725 1103 19 0.14347493240122589 1103 20 -0.40790643921508973 1103 21 -0.11989122366428973 1103 22 -0.29158853289818154 1103 23 0.1631310604112528 1103 24 0.1876375431233157 1103 25 0.14045395175666897 1104 1 0.5665338464650453 1104 2 0.6912203527221976 1104 3 0.8414200769279603 1104 4 0.8591785857150498 1104 5 0.6107409347475041 1104 6 0.5588951370818549 1104 7 0.8086836711456229 1104 8 0.6851168605568994 1104 9 0.8055387073430182 1104 10 0.7513468836732855 1104 11 0.8195611375512115 1104 12 0.6662199253153602 1104 13 0.7358053660754464 1104 14 0.836716056227246 1104 15 0.9566331571443101 1104 16 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0.4487010008251673 2207 7 0.31353193702035753 2207 8 0.44541483577067353 2207 9 -0.7316031804404091 2207 10 -0.13705194017060762 2207 11 0.4884103316610293 2207 12 0.4988328483452804 2207 13 0.6804160213917925 2207 14 0.873929177241493 2207 15 0.7600235651858824 2207 16 0.9112376865614351 2207 17 0.7304036521217796 2207 18 0.7551544241215353 2207 19 -0.2566496523163835 2207 20 0.3394772817151406 2207 21 -0.4060830841294063 2207 22 0.5032204372014846 2207 23 -0.40327259407379756 2207 24 -1.3910584701657243 2207 25 0.008601792288224885 2208 1 0.3348500285743299 2208 2 0.010443844359482313 2208 3 0.36657339518472654 2208 4 0.22014070529786167 2208 5 0.283598986915905 2208 6 -0.10673528716926484 2208 7 0.2510266652127555 2208 8 0.27196896465038023 2208 9 -0.3251277681510416 2208 10 -0.01834443774217931 2208 11 0.12214826772454757 2208 12 -0.09542911219574535 2208 13 0.25777826735869525 2208 14 0.78252079513544 2208 15 0.5282327287438804 2208 16 0.23773875724855031 2208 17 0.21759077884379252 2208 18 0.46774975880989444 2208 19 -1.4620372356109428 2208 20 -0.32676832057697425 2208 21 -0.3476619326849999 2208 22 -0.05283078164043076 2208 23 0.19961608439627554 2208 24 0.14223418521995512 2208 25 0.2328182316209464 2209 1 0.06057096482158286 2209 2 -1.008975096112092 2209 3 0.08593629875815623 2209 4 0.10955404207961905 2209 5 0.015311560733742624 2209 6 0.020640892838500235 2209 7 0.03170686694559066 2209 8 0.22058743958464433 2209 9 -2.0596206342228562 2209 10 -0.43247866766588317 2209 11 -0.16795044342679688 2209 12 0.1994758406039452 2209 13 0.057748093644306664 2209 14 0.5344282049375835 2209 15 0.6459964254549462 2209 16 0.49928020300052367 2209 17 0.3914775843095484 2209 18 0.5037211368865329 2209 19 0.2341134605397528 2209 20 0.38895218461721726 2209 21 0.2660067599310594 2209 22 0.14202675532236952 2209 23 0.46066181912622284 2209 24 0.4770115463131077 2209 25 0.5554830016541388 2210 1 -0.2670592913444665 2210 2 -0.24111943800948765 2210 3 -0.17649595594149758 2210 4 0.053177102315302704 2210 5 -0.29736370779086485 2210 6 -0.19592486451049496 2210 7 -0.17539911429359023 2210 8 -0.07647900939000962 2210 9 0.15363061108126885 2210 10 -1.6575706954518488 2210 11 -0.7964038475100281 2210 12 -2.256339484006592 2210 13 -1.1429518466415622 2210 14 -0.9861580517424642 2210 15 -1.0967755194871174 2210 16 -0.4645738526469998 2210 17 -0.2625319548334417 2210 18 -0.13180347818410354 2210 19 0.1796828990001842 2210 20 -0.0783335437061794 2210 21 0.1485399995358918 2210 22 0.09367720846945815 2210 23 0.15475541157351813 2210 24 0.16443454154353532 2210 25 0.2329133288724421 2211 1 -0.4528072599176802 2211 2 -0.28011412900316657 2211 3 -1.991874140224411 2211 4 -0.9727428748979072 2211 5 -0.3353126854181383 2211 6 -0.7010031018043534 2211 7 -0.3782134414897875 2211 8 -0.1811360602409522 2211 9 -0.19697875102038864 2211 10 -0.2962707932979143 2211 11 -0.49886081238302893 2211 12 -0.3264365652399306 2211 13 -0.49589527262536137 2211 14 -0.8760139727482468 2211 15 -0.5860876167951616 2211 16 -0.4229932298607295 2211 17 -0.5538020284421705 2211 18 -0.2767356584635081 2211 19 -0.36306871176762456 2211 20 -0.34667045066274227 2211 21 -0.5197525997673225 2211 22 -0.6067358021564715 2211 23 -0.6695871604330108 2211 24 -0.6016988508666685 2211 25 -0.28696973057102326 2212 1 -0.9573322688481412 2212 2 -0.8566806044243549 2212 3 -2.8795603129577145 2212 4 -2.4976745295479397 2212 5 -0.9925988464871693 2212 6 -1.1148194332446728 2212 7 -0.8669552309350799 2212 8 -1.0842606273870707 2212 9 -1.5333027553653338 2212 10 -0.8149132177170699 2212 11 -0.943947601864771 2212 12 -0.5982112114843972 2212 13 -1.125265912192038 2212 14 -0.9203185269178604 2212 15 -0.36440592022664475 2212 16 -1.3852848221069038 2212 17 -1.434840294547982 2212 18 -0.7422334677672211 2212 19 -0.7235946246792508 2212 20 -0.6592554722780974 2212 21 -0.4057029828657903 2212 22 -0.6637267113136502 2212 23 -0.49885427957627115 2212 24 -0.6037925878810526 2212 25 -0.15247337201126498 2213 1 -3.430669608896526 2213 2 -1.53040061111912 2213 3 -2.675869824087762 2213 4 -1.451743881220871 2213 5 -0.9402800235484712 2213 6 -0.8052695039961613 2213 7 -0.6431774058205839 2213 8 -0.4358346577873853 2213 9 -0.8920439875062876 2213 10 -0.5597688998983443 2213 11 -0.6776823674719381 2213 12 -0.439563553891162 2213 13 -0.8216705194071672 2213 14 -0.5116959746910865 2213 15 -0.45395828393493787 2213 16 -1.5293668025399363 2213 17 -1.1036251948112081 2213 18 -0.6437192490005756 2213 19 -0.5553491094106207 2213 20 -0.629204348622757 2213 21 -0.1090244989256135 2213 22 -0.12116170362988754 2213 23 0.19131050291996132 2213 24 0.07156611455188511 2213 25 -0.08188925381824674 2214 1 -0.22864786482046995 2214 2 -0.35930969549715286 2214 3 -5.777642517072422 2214 4 -1.461065336732922 2214 5 -0.9994206775470609 2214 6 -0.3407922119478357 2214 7 -0.059383088495962966 2214 8 0.12749975317086282 2214 9 0.2261864326476939 2214 10 0.129590751430122 2214 11 -0.21462993503303807 2214 12 -0.009315819896890787 2214 13 -3.9545835790237724 2214 14 -2.877112596456719 2214 15 -1.0317358621525443 2214 16 -2.1031302279503308 2214 17 -2.58676934510396 2214 18 -0.3499676774642424 2214 19 -0.08563036951335334 2214 20 0.2818886025129359 2214 21 0.05797144202053772 2214 22 0.12791932179008825 2214 23 0.44097224843284977 2214 24 0.5310653781748655 2214 25 0.46080864002372834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 206.0 3 38.0 4 29.0 5 62.0 6 9.0 7 9.0 8 21.0 9 74.0 10 232.0 11 773.0 12 1929.0 13 3609.0 14 5895.0 15 8515.0 16 10807.0 17 12208.0 18 12179.0 19 11384.0 20 10440.0 21 9494.0 22 8628.0 23 7755.0 24 7147.0 25 6751.0 26 6742.0 27 6892.0 28 7481.0 29 8832.0 30 10831.0 31 13498.0 32 17468.0 33 23491.0 34 31670.0 35 43485.0 36 61269.0 37 91584.0 38 148411.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.665206774668135 21.73479967351815 12.7523064992627 19.847687052551024 2 17.1715852481567 24.7189886815239 36.66453515885387 21.444890911465535 3 19.895208031902353 31.641814336043318 25.909975252269106 22.553002379785227 4 12.171073833471853 22.92467391488712 32.34170666757128 32.562545584069746 5 13.426517206112077 42.24448639471359 28.54608395368283 15.782912445491505 6 27.269056751653626 40.493805378793915 16.626488855110498 15.610649014441957 7 24.450249790074555 39.683627511683724 17.864443294684435 18.001679403557283 8 23.800064799469393 38.22897282663089 19.105156646780127 18.865805727119593 9 24.243992142200387 22.546897424501 18.89095097696606 34.31815945633255 10 17.659033424203923 34.11972864357411 24.990712200351833 23.23052573187013 11 29.19286055971529 33.273001150099915 18.286249351160446 19.24788893902435 12 20.68746745106421 34.266162508036516 24.49953095605236 20.546839084846912 13 27.166089630827116 29.37437553752542 23.457567085717386 20.001967745930074 14 20.921983409387774 31.228689205923764 24.466233523893536 23.383093860794922 15 24.834301709147972 32.508248865515924 22.884091776580856 19.773357648755248 16 20.0419339133739 33.88300913148284 22.402073625230916 23.672983329912352 17 19.812581851246176 32.578831656160304 23.606577956314336 24.002008536279188 18 19.772890887111014 29.99925293054971 27.61457328751307 22.613282894826202 19 21.5590773991287 30.838524490045195 28.065327144660234 19.537070966165874 20 23.129172073928938 26.891948056354277 29.182421944578742 20.796457925138046 21 25.318066157760814 28.256318914334184 26.747582697201018 19.678032230703987 22 24.119240732666853 28.415184668079252 26.521596775370128 20.94397782388377 23 23.737094426556556 28.378011583208078 27.65194054360328 20.23295344663208 24 23.221590147552583 27.52635231084275 27.522789468237256 21.72926807336741 25 22.500839754486325 29.922403292516176 27.808257755429565 19.768499197567934 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4341.0 1 4341.0 2 3923.5 3 3506.0 4 3506.0 5 3506.0 6 4202.5 7 4899.0 8 4899.0 9 4899.0 10 5291.0 11 5683.0 12 5683.0 13 5683.0 14 6326.5 15 6970.0 16 6970.0 17 6970.0 18 9461.0 19 11952.0 20 11952.0 21 11952.0 22 15770.0 23 19588.0 24 19588.0 25 19588.0 26 26623.5 27 33659.0 28 33659.0 29 33659.0 30 38702.0 31 43745.0 32 43745.0 33 43745.0 34 47100.0 35 50455.0 36 50455.0 37 50455.0 38 49118.5 39 47782.0 40 47782.0 41 47782.0 42 52640.5 43 57499.0 44 57499.0 45 57499.0 46 69555.5 47 81612.0 48 81612.0 49 81612.0 50 76405.5 51 71199.0 52 71199.0 53 71199.0 54 62639.5 55 54080.0 56 54080.0 57 54080.0 58 45346.5 59 36613.0 60 36613.0 61 36613.0 62 33836.0 63 31059.0 64 31059.0 65 31059.0 66 21455.5 67 11852.0 68 11852.0 69 11852.0 70 10120.5 71 8389.0 72 8389.0 73 8389.0 74 5937.0 75 3485.0 76 3485.0 77 3485.0 78 2298.0 79 1111.0 80 1111.0 81 1111.0 82 682.5 83 254.0 84 254.0 85 254.0 86 161.5 87 69.0 88 69.0 89 69.0 90 49.5 91 30.0 92 30.0 93 30.0 94 21.0 95 12.0 96 12.0 97 12.0 98 8.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0907013332248308 2 0.04763939184332235 3 0.0506910254845316 4 0.04713078623645414 5 0.04424868779753428 6 0.0652710528814203 7 0.05984592640815939 8 0.057133363171528936 9 0.06272802484707925 10 0.06188034883563223 11 0.056624757564660726 12 0.059506856003580584 13 0.05781150398068655 14 0.060693602419606404 15 0.06323663045394745 16 0.05916778559900178 17 0.061710813633342826 18 0.14919097801467499 19 0.06510151767913089 20 0.06154127843105343 21 0.05899825039671237 22 0.0652710528814203 23 0.06340616565623686 24 0.07306967218673285 25 0.06611872889286731 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 589848.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.91660184851146 #Duplication Level Percentage of deduplicated Percentage of total 1 83.7624239821781 40.13610719820237 2 9.87863487121277 9.467012278614472 3 2.9309169256703598 4.213187381352298 4 1.1798196231325446 2.26131788538812 5 0.5648492532835195 1.3532828387007707 6 0.33946071967731584 0.9759482488792264 7 0.2007614099578452 0.6733863183247096 8 0.1399081147915975 0.5363137145475854 9 0.1093418739365542 0.4715361934909201 >10 0.6651890160487828 5.928000249509145 >50 0.08990786425317969 2.9941151366687935 >100 0.10774672608988814 11.599161684267282 >500 0.01819563917412018 6.270306891119329 >1k 0.011416871638663643 8.825408226924703 >5k 0.0014271089548329554 4.29491575401033 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8827 1.4964872306085635 No Hit GGTATCAACGCAGAGTACTTTTTTT 5875 0.9960193134502449 No Hit GTACTGGTTCACTATCGGTCAGTCA 5290 0.8968412201109438 No Hit TATCAACGCAGAGTACTTTTTTTTT 5131 0.8698851229469288 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3733 0.6328749101463428 No Hit ATACAGGGTGACAGCCCCGTACACA 3681 0.6240590796272938 No Hit GGATACCACGTGTCCCGCCCTACTC 3199 0.5423431121238014 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 2301 0.39010050046791717 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2140 0.3628053328993232 No Hit GTTAATGATAGTGTGTCGAAACACA 1992 0.3377141229604915 No Hit GTTCACTATCGGTCAGTCAGGAGTA 1919 0.32533805319336506 No Hit GTATTTAGCCTTGGAGGATGGTCCC 1777 0.30126405446826976 No Hit CCCATATTCAGACAGGATACCACGT 1594 0.270239112449309 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 1583 0.2683742252241256 No Hit GGTTAATGAGGCGAACCGGGGGAAC 1520 0.2576935074798931 No Hit GCCTTGGAGGATGGTCCCCCCATAT 1504 0.2549809442432627 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 1458 0.24718232493795012 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 1455 0.24667371933108193 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 1446 0.24514790251047727 No Hit GGTATCAACGCAGAGTACATGGGGG 1443 0.24463929690360905 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 1437 0.24362208568987262 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1380 0.23395857915937665 No Hit CTATCGGTCAGTCAGGAGTATTTAG 1339 0.2270076358655111 No Hit ATCATTAACTGAATCCATAGGTTAA 1311 0.2222606502014078 No Hit TATTCAGACAGGATACCACGTGTCC 1277 0.2164964533235681 No Hit TCACGGTACTGGTTCACTATCGGTC 1203 0.20395084835415225 No Hit CACACACACTGATTCAGGCTCTGGG 1200 0.20344224274728404 No Hit CGGTACTGGTTCACTATCGGTCAGT 1195 0.20259456673583703 No Hit CATCTAAGTACCCCGAGGAAAAGAA 1156 0.1959826938465503 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1103 0.18699732812521191 No Hit AGCGTACACGGTGGATGCCCTGGCA 1093 0.18530197610231788 No Hit GTACATGGGGGTTAAGCGACTAAGC 1057 0.17919870881989935 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 1054 0.17869010321303114 No Hit GGTTCACTATCGGTCAGTCAGGAGT 1040 0.1763166103809795 No Hit TTCCAGACGCTTCCACTAACACACA 1025 0.17377358234663845 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 1009 0.171061019110008 No Hit GTATCAACGCAGAGTACATGGGGGT 999 0.16936566708711395 No Hit AGCTCACAGCATGTGCATTTTTGTG 991 0.16800938546879873 No Hit TCATTAACCTATGGATTCAGTTAAT 982 0.1664835686481941 No Hit GTACTTAGATGTTTCAGTTCCCCCG 981 0.1663140334459047 No Hit GTCCCGCCCTACTCATCGAGCTCAC 964 0.16343193500698486 No Hit GGGTTTCCCCATTCGGAAATCGCCG 933 0.15817634373601336 No Hit GGGTGACAGCCCCGTACACAAAAAT 902 0.15292075246504186 No Hit GGAGTATTTAGCCTTGGAGGATGGT 881 0.1493605132169644 No Hit GTGCTAATCTGCGATAAGCGTCGGT 866 0.14681748518262333 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 853 0.14461352755286108 No Hit GCCCTACTCATCGAGCTCACAGCAT 843 0.14291817552996702 No Hit ACGCAGAGTACATGGGGGTTAAGCG 841 0.14257910512538824 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 839 0.14224003472080943 No Hit GGTATCAACGCAGAGTACATGGGGA 839 0.14224003472080943 No Hit GGGTACTTAGATGTTTCAGTTCCCC 823 0.13952747148417896 No Hit GTCCCCCCATATTCAGACAGGATAC 821 0.13918840107960015 No Hit CCTATGGATTCAGTTAATGATAGTG 803 0.1361367674383909 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 785 0.13308513379718165 No Hit GTGTGTGTTAGTGGAAGCGTCTGGA 778 0.1318983873811558 No Hit ATCAACGCAGAGTACTTTTTTTTTT 774 0.1312202465719982 No Hit GTGGTATCAACGCAGAGTACATGGG 771 0.13071164096513 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 758 0.12850768333536775 No Hit ATGATAGTGTGTCGAAACACACTGG 751 0.12732093691934193 No Hit GTTATAACCGGCGATTTCCGAATGG 740 0.1254560496941585 No Hit GCTCTGGGCTGCTCCCCGTTCGCTC 731 0.12393023287355387 No Hit CTACTGGGGGAATCTCGGTTGATTT 717 0.12155674004150221 No Hit GTATCGCGCGCCTTTCCAGACGCTT 712 0.1207090640300552 No Hit AGTCAGGAGTATTTAGCCTTGGAGG 703 0.11918324720945057 No Hit GTGTGTTAGTGGAAGCGTCTGGAAA 699 0.11850510640029296 No Hit CACTATCATTAACTGAATCCATAGG 685 0.11613161356824131 No Hit TCCCCCAGTAGCGGCGAGCGAACGG 670 0.11358858553390026 No Hit TTTGTGTACGGGGCTGTCACCCTGT 662 0.11223230391558504 No Hit GTGGTATCAACGCAGAGTACTTTTT 655 0.11104555749955922 No Hit GGTATCAACGCAGAGTACATGGGCA 605 0.10256879738508905 No Hit TAAGTACCCCGAGGAAAAGAAATCA 597 0.1012125157667738 No Hit GATATGAACCGTTATAACCGGCGAT 595 0.100873445362195 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 25 0.006040808 18.992874 1 TCTATAC 35 9.961424E-5 18.991262 3 CTATACC 35 0.0021747225 16.278225 4 GGTATCA 1515 0.0 16.172148 1 GTACAAA 120 0.0 15.036024 1 CCGAATG 165 0.0 14.983151 18 GTCTATA 45 6.7771156E-4 14.770981 2 AATCGCC 155 0.0 14.722899 18 ACTTAAC 40 0.0052485205 14.2603855 16 TACCCTG 40 0.0052900785 14.243445 7 AGGACCG 40 0.0052900785 14.243445 5 TACACTC 40 0.0052900785 14.243445 5 GTAACAA 75 9.684281E-7 13.928106 1 GAAATCG 175 0.0 13.58132 16 ATCGCCG 175 0.0 13.567489 19 AAATCGC 170 0.0 13.421539 17 GTTTAGA 50 0.0015018613 13.2950115 1 CGAATGG 195 0.0 12.66299 19 CGCAGAA 85 3.9518636E-6 12.288464 2 GGTTATA 125 1.4097168E-9 12.155437 1 >>END_MODULE