FastQCFastQC Report
Wed 25 May 2016
SRR1294652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589848
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT241184.088850008815831No Hit
GGTATCAACGCAGAGTACTTTTTTT174592.9599150967706933No Hit
TATCAACGCAGAGTACTTTTTTTTT153392.600500467917158No Hit
ACGCAGAGTACTTTTTTTTTTTTTT85841.4552901764522386No Hit
GTACTGGTTCACTATCGGTCAGTCA48680.8252973647448156No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45030.7634170159091833No Hit
ATACAGGGTGACAGCCCCGTACACA40820.6920426957453446No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37890.6423688814745494No Hit
GGATACCACGTGTCCCGCCCTACTC32790.5559059283069536No Hit
GCAGAGTACTTTTTTTTTTTTTTTT30630.5192863246124425No Hit
ACCCTGTATCGCGCGCCTTTCCAGA25760.4367226810975031No Hit
ATCAACGCAGAGTACTTTTTTTTTT23850.4043414574602271No Hit
CCCATATTCAGACAGGATACCACGT19070.3233036307658922No Hit
GTTAATGATAGTGTGTCGAAACACA18700.3170308282811843No Hit
GTGGTATCAACGCAGAGTACTTTTT17960.30448522331176847No Hit
GTTCACTATCGGTCAGTCAGGAGTA17260.2926177591515102No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG16470.2792244781706474No Hit
GCCTTGGAGGATGGTCCCCCCATAT15600.26447491557146924No Hit
ACTTAGATGTTTCAGTTCCCCCGGT15570.2639663099646011No Hit
CTATCGGTCAGTCAGGAGTATTTAG15110.2561676906592885No Hit
GGTATCAACGCAGAGTACATGGGGG15040.2549809442432627No Hit
GTCAGGAGTATTTAGCCTTGGAGGA14940.2532855922203686No Hit
GCCCAGAGCCTGAATCAGTGTGTGT14920.2529465218157898No Hit
TCTAAGTACCCCGAGGAAAAGAAAT14780.2505730289837382No Hit
GTATTTAGCCTTGGAGGATGGTCCC14710.2493862825677124No Hit
CGGTACTGGTTCACTATCGGTCAGT14090.23887510002576934No Hit
CATCTAAGTACCCCGAGGAAAAGAA13800.23395857915937665No Hit
CACACACACTGATTCAGGCTCTGGG13620.2309069455181674No Hit
GGTTAATGAGGCGAACCGGGGGAAC13560.229889734304431No Hit
TCACGGTACTGGTTCACTATCGGTC13470.22836391748382634No Hit
ATCATTAACTGAATCCATAGGTTAA13220.22412553742659128No Hit
AGCGTACACGGTGGATGCCCTGGCA13160.22310832621285484No Hit
TATTCAGACAGGATACCACGTGTCC12360.2095455100297026No Hit
CCCCAGTAGCGGCGAGCGAACGGGG11340.19225291939618344No Hit
AGCTCACAGCATGTGCATTTTTGTG11300.19157477858702582No Hit
CAACGCAGAGTACTTTTTTTTTTTT10950.1856410465068967No Hit
GGTTCACTATCGGTCAGTCAGGAGT10480.17767289199929473No Hit
GTACTTAGATGTTTCAGTTCCCCCG10210.17309544153748083No Hit
TCATTAACCTATGGATTCAGTTAAT10210.17309544153748083No Hit
GTGGATGCCCTGGCAGTCAGAGGCG10210.17309544153748083No Hit
GCCTGAATCAGTGTGTGTGTTAGTG10040.17021334309856098No Hit
GTATCAACGCAGAGTACATGGGGGT9980.16919613188482457No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9760.1654663574344577No Hit
GTACATGGGGGTTAAGCGACTAAGC9680.16411007581614245No Hit
GGGTGACAGCCCCGTACACAAAAAT9600.16275379419782723No Hit
AACGCAGAGTACTTTTTTTTTTTTT9500.1610584421749332No Hit
GGTATCAACGCAGAGTACATGGGGA9480.1607193717703544No Hit
GGGTTTCCCCATTCGGAAATCGCCG9220.15631145651082992No Hit
ACGCAGAGTACATGGGGGTTAAGCG9100.15427703408335708No Hit
GGAGTATTTAGCCTTGGAGGATGGT9060.15359889327419946No Hit
GTCCCGCCCTACTCATCGAGCTCAC9050.15342935807191005No Hit
GCCCTACTCATCGAGCTCACAGCAT9020.15292075246504186No Hit
GTGGTATCAACGCAGAGTACATGGG8920.15122540044214783No Hit
CCTATGGATTCAGTTAATGATAGTG8900.15088633003756902No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG8530.14461352755286108No Hit
TTCCAGACGCTTCCACTAACACACA8320.1410532883047836No Hit
TCAACGCAGAGTACTTTTTTTTTTT8140.13800165466357434No Hit
GTGCTAATCTGCGATAAGCGTCGGT8110.13749304905670612No Hit
GGGTACTTAGATGTTTCAGTTCCCC8010.1357976970338121No Hit
CAGTAGCGGCGAGCGAACGGGGAGC7990.13545862662923328No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA7890.13376327460633924No Hit
GAATCAGTGTGTGTGTTAGTGGAAG7610.12901628894223596No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA7530.12766000732392074No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC7460.1264732609078949No Hit
GTCCCCCCATATTCAGACAGGATAC7390.1252865144918691No Hit
ATGATAGTGTGTCGAAACACACTGG7320.12409976807584326No Hit
GTTATAACCGGCGATTTCCGAATGG7230.12257395125523864No Hit
TCCCCCAGTAGCGGCGAGCGAACGG7090.120200458423187No Hit
AGTCAGGAGTATTTAGCCTTGGAGG6990.11850510640029296No Hit
CTACTGGGGGAATCTCGGTTGATTT6830.11579254316366251No Hit
AACACACACACTGATTCAGGCTCTG6750.11443626154534728No Hit
CACTATCATTAACTGAATCCATAGG6710.11375812073618967No Hit
CTGTCACCCTGTATCGCGCGCCTTT6450.10935020547666519No Hit
GGTATCAACGCAGAGTACATGGGCA6390.10833299426292876No Hit
CTAACACACACACTGATTCAGGCTC6360.10782438865606055No Hit
GATATGAACCGTTATAACCGGCGAT6320.10714624784690294No Hit
TAAGTACCCCGAGGAAAAGAAATCA6180.1047727550148513No Hit
CATTAACCTATGGATTCAGTTAATG6110.10358600859882547No Hit
GTATCGCGCGCCTTTCCAGACGCTT6090.10324693819424666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACA453.5925113E-516.85595717
GGTATCA29750.016.2981721
TAATACC400.00519420314.2827564
CAGCGTA602.5100195E-514.28154110
GCACGTC759.39679E-713.9653616
ACGTCCT707.074377E-613.6026258
CAACCCC707.391789E-613.54496712
CTGCTTG500.001519810813.27406813
CGCAGCG655.325267E-513.1840828
CGAACAC655.541853E-513.12819918
AATCGCC1450.013.07789918
GAAATCG1550.012.84587316
ATCGCCG1501.8189894E-1212.64196919
AAGGCAA905.514903E-712.64196819
CGTCCTT853.835543E-612.3213289
ACGCAGC701.065587E-412.2423627
GCAGCGT701.06643456E-412.241329
GTACAAA1103.7553036E-812.1001591
TCCAAAG550.003060656812.0929632
CGTACTT550.003109686512.06733413
Produced by FastQC (version 0.11.5)