FastQCFastQC Report
Wed 25 May 2016
SRR1294651_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294651_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5260479
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT121480.23092954082698552No Hit
GGTATCAACGCAGAGTACTTTTTTT74940.142458509956983No Hit
TATCAACGCAGAGTACTTTTTTTTT68290.12981707559330624No Hit
GTACATGGGAAGCAGTGGTATCAAC64740.12306864070743369No Hit
CCCATGTACTCTGCGTTGATACCAC60990.11594001230686407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA8000.011.40520916
TGTCCCG7850.011.37179211
AGCGTCG2550.010.80906417
ATCGCCG1158.857969E-710.73647419
ATACCGC803.7835282E-410.6845526
GTCCCGC8350.010.5851712
CGTCGGT2700.010.55294519
TGCGCTA1201.5319838E-610.28843610
TCCCGCC8900.010.251677513
CCGCCCT9100.010.235249515
CCCGCCC9050.010.0817614
GTCTTAC4950.09.9803691
TATACTG12850.09.9037885
CCGTACA6550.09.8672917
GCGTCGG2900.09.83263918
CGGGGGA4850.09.60250717
TCGCGTT800.00453018639.49701810
GCGCTAT1106.8694215E-59.49665611
CCCGTAC7100.09.23661316
CTAGTAC3850.09.1272653
Produced by FastQC (version 0.11.5)