##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294651_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5260479 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.293992809400056 31.0 30.0 34.0 26.0 34.0 2 30.29661101203902 31.0 30.0 34.0 26.0 34.0 3 30.29468875362871 31.0 30.0 34.0 26.0 34.0 4 33.943391847016215 35.0 35.0 37.0 28.0 37.0 5 34.27513578136136 37.0 35.0 37.0 30.0 37.0 6 34.37885770478316 37.0 35.0 37.0 30.0 37.0 7 34.236596895453815 37.0 35.0 37.0 30.0 37.0 8 34.13591537956905 37.0 35.0 37.0 29.0 37.0 9 35.253239676462925 39.0 35.0 39.0 28.0 39.0 10 35.335053899084095 39.0 35.0 39.0 28.0 39.0 11 35.5961869632024 39.0 35.0 39.0 29.0 39.0 12 35.362321948248436 39.0 35.0 39.0 27.0 39.0 13 35.241580281947705 39.0 35.0 39.0 27.0 39.0 14 36.15129287656124 39.0 36.0 41.0 27.0 41.0 15 36.15042907689585 39.0 36.0 41.0 27.0 41.0 16 36.264756688506885 39.0 36.0 41.0 27.0 41.0 17 36.242375456683696 39.0 36.0 41.0 27.0 41.0 18 36.11621147047636 39.0 36.0 41.0 27.0 41.0 19 36.39581642660298 39.0 36.0 41.0 27.0 41.0 20 36.50581629543621 39.0 36.0 41.0 27.0 41.0 21 36.54321688196075 39.0 36.0 41.0 27.0 41.0 22 36.53707314486 39.0 36.0 41.0 28.0 41.0 23 36.50442250601134 39.0 36.0 41.0 27.0 41.0 24 36.551103616229625 39.0 36.0 41.0 28.0 41.0 25 35.3116092660003 39.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.055698909980837 1101 2 1.0604110281993755 1101 3 1.319579382058631 1101 4 1.1373194044853108 1101 5 0.8327340482585797 1101 6 0.7923484993043672 1101 7 0.8508028802019538 1101 8 0.9776907007608955 1101 9 1.3148771810166195 1101 10 1.235378827773026 1101 11 0.9858382051549555 1101 12 1.2102564170985772 1101 13 1.269095285931762 1101 14 1.3967588201194587 1101 15 1.5077612481718319 1101 16 1.4529942047968945 1101 17 1.4365079716576261 1101 18 1.488595687638103 1101 19 1.3264448084572464 1101 20 1.2275027467068398 1101 21 1.1369147815626022 1101 22 1.0800742572561504 1101 23 1.0688756608133403 1101 24 1.1453761592043108 1101 25 1.1721381969362454 1102 1 0.9531381278844258 1102 2 0.9120874871956985 1102 3 1.1360190369097403 1102 4 1.0029765188394748 1102 5 0.8332842770017947 1102 6 0.6909487605212732 1102 7 0.7074284637568624 1102 8 0.8438790116613504 1102 9 1.1565038073773195 1102 10 0.9879386979100033 1102 11 0.8036502796564875 1102 12 0.9373351096818112 1102 13 0.9938914629978086 1102 14 1.2207793558599391 1102 15 1.346738909923701 1102 16 1.2151953100917368 1102 17 1.2158399970560936 1102 18 1.292907907656641 1102 19 1.0324557313522718 1102 20 0.3418969954941318 1102 21 0.5253045070310947 1102 22 0.7580295411094724 1102 23 0.8616438621359208 1102 24 0.8860347207984489 1102 25 0.9657050764727515 1103 1 0.7169484952064629 1103 2 0.7301962976227756 1103 3 1.0378137382983326 1103 4 0.8174119129790185 1103 5 0.5926190537948699 1103 6 0.5033785966028361 1103 7 0.4198372964608481 1103 8 0.6447587828029029 1103 9 0.7080595855495204 1103 10 0.6802255895492237 1103 11 0.6770263237761185 1103 12 0.8849416259013339 1103 13 0.7631652555436048 1103 14 0.6550246380840079 1103 15 0.9256948676826156 1103 16 0.9559674702714887 1103 17 0.9208523002939302 1103 18 0.9899456281161818 1103 19 0.7969222778895428 1103 20 -0.07145590180621042 1103 21 0.37606451777573113 1103 22 -0.25576888446010315 1103 23 0.32155971931373273 1103 24 0.479366471694469 1103 25 0.535227823698861 1104 1 0.3802681209035299 1104 2 0.34886278981829477 1104 3 0.6294641790966793 1104 4 0.5475206054588 1104 5 0.3991970876477424 1104 6 0.27458566650094696 1104 7 0.2048322578032682 1104 8 0.4393379157388466 1104 9 0.6908442689285152 1104 10 0.6472913799325539 1104 11 0.3657526458208551 1104 12 0.47262943173065963 1104 13 0.42687381242051714 1104 14 0.7301275479329519 1104 15 0.7764117761044673 1104 16 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0.3832321204033846 2207 7 0.36433900502647276 2207 8 0.3776146366840365 2207 9 -1.219981923447591 2207 10 -0.11445333049551465 2207 11 0.15296160163615014 2207 12 0.4982003904234844 2207 13 0.6254347648280856 2207 14 0.8219207476746817 2207 15 0.9020857111041991 2207 16 0.8194020291225499 2207 17 0.7785403243601081 2207 18 0.9085496865709572 2207 19 -0.3558762941869489 2207 20 0.295378927167782 2207 21 -0.21718723927256178 2207 22 0.31038091376942845 2207 23 -0.4637554828070307 2207 24 -1.4922078695843481 2207 25 -0.12496584248928855 2208 1 0.5308789743748967 2208 2 0.24206399079198704 2208 3 0.7259534942122308 2208 4 0.6089744774977532 2208 5 0.4803615185288308 2208 6 0.3536301922372047 2208 7 0.4938108045733216 2208 8 0.5998127027646589 2208 9 -0.001110129949147165 2208 10 0.18765865841955787 2208 11 0.41438831619312566 2208 12 0.3937393877757316 2208 13 0.6615073993468528 2208 14 0.9480280196052249 2208 15 0.9086509128385245 2208 16 0.634736544619031 2208 17 0.6865481169298846 2208 18 0.907537597540859 2208 19 -1.2071686175965155 2208 20 0.05135671556043775 2208 21 -0.11553003013949592 2208 22 0.31034044461756594 2208 23 0.4624997853214836 2208 24 0.5878481609492496 2208 25 0.6935535583570953 2209 1 0.12984766929368874 2209 2 -1.4092663806486918 2209 3 -0.25439745000026903 2209 4 0.050925736286465906 2209 5 0.10286452111504474 2209 6 0.12472359103112751 2209 7 0.16405255218676018 2209 8 0.2650639453107573 2209 9 -2.1096348936010045 2209 10 -0.38927568173309623 2209 11 -0.035109814513766935 2209 12 0.3857788617851483 2209 13 0.36626705285908656 2209 14 0.571723319223679 2209 15 0.7025689186854507 2209 16 0.6428600450229354 2209 17 0.473339518940449 2209 18 0.607393032700557 2209 19 0.3547845474193281 2209 20 0.3398731208449348 2209 21 0.4038810344437991 2209 22 0.33132422490824354 2209 23 0.38027361975723295 2209 24 0.33545583023430225 2209 25 0.37292692065004474 2210 1 0.15738371801475282 2210 2 0.19881109075936365 2210 3 0.16090170695639472 2210 4 0.2863967581012119 2210 5 0.22510894195141162 2210 6 0.12280878894029712 2210 7 0.1688400353453261 2210 8 0.2427206222013325 2210 9 0.38780920701083943 2210 10 -1.3373586185918427 2210 11 -0.36376606686214785 2210 12 -2.2517094706037923 2210 13 -0.7109689522120632 2210 14 -0.3349026238567987 2210 15 -0.6867491613688728 2210 16 -0.023698835058802104 2210 17 0.17527184634444382 2210 18 0.4995006250541323 2210 19 0.33806982343813985 2210 20 0.3347277799678565 2210 21 0.31363370959143566 2210 22 0.2693160033743851 2210 23 0.18365444801006703 2210 24 0.1911326778496516 2210 25 0.13545705031997812 2211 1 -0.3060566199424848 2211 2 -0.07101124247021673 2211 3 -1.3712699161886448 2211 4 -0.516706564114024 2211 5 -0.3638697291571731 2211 6 -0.4036200968866126 2211 7 -0.3794253958559608 2211 8 -0.08675925189505307 2211 9 0.10827563187974931 2211 10 0.13254040929180633 2211 11 -0.28429741078841886 2211 12 -0.23355972413110493 2211 13 -0.3510281793332979 2211 14 -0.819531638271755 2211 15 -0.26324682888749606 2211 16 -0.0769516995507189 2211 17 -0.17826711374958393 2211 18 0.028125508226302998 2211 19 -0.0623307432626774 2211 20 -0.004993953779219851 2211 21 -0.1316534400780185 2211 22 -0.17715385492076763 2211 23 -0.5220037647541105 2211 24 -0.2966396866311527 2211 25 -0.08584434512310679 2212 1 -0.3999615092314244 2212 2 -0.35029774202939734 2212 3 -2.435933471293108 2212 4 -1.374833269618975 2212 5 -0.6071542524100835 2212 6 -0.41528706650728964 2212 7 -0.3078418526882061 2212 8 -0.3595396462481375 2212 9 -0.9632053605448263 2212 10 -0.2761084718219564 2212 11 -0.3450520259537342 2212 12 -0.07014980152393946 2212 13 -0.40660443327476514 2212 14 -0.01430792420738669 2212 15 -0.009144347853833779 2212 16 -1.1730818774779905 2212 17 -1.4610029148458565 2212 18 -0.260248431359436 2212 19 -0.1112312701527074 2212 20 -0.24723602824536073 2212 21 -0.040065072790000045 2212 22 -0.3400517875616629 2212 23 -0.12641104437103934 2212 24 -0.18024771755550972 2212 25 -0.08920463511466892 2213 1 -3.151595652409476 2213 2 -1.383419948730893 2213 3 -2.4713599666130186 2213 4 -1.1100993334704299 2213 5 -0.8875423598502579 2213 6 -0.6446717397035826 2213 7 -0.4176885719894301 2213 8 -0.28893357988047796 2213 9 -0.3289638058773363 2213 10 -0.21623136335297488 2213 11 -0.4849678217770119 2213 12 -0.28037072023243326 2213 13 -0.653037753366398 2213 14 -0.2899000526464093 2213 15 -0.3268672914551516 2213 16 -1.6545173514071863 2213 17 -1.0919990963228443 2213 18 -0.443523529391058 2213 19 -0.2107310969539995 2213 20 -0.22547617588931956 2213 21 -0.03570620250774681 2213 22 -0.12122822168841907 2213 23 -0.033155163813120225 2213 24 -0.06497097110006678 2213 25 -0.03785105873571126 2214 1 -1.0059043386279747 2214 2 -0.7796628305698405 2214 3 -6.318863960189631 2214 4 -2.1391433032118243 2214 5 -1.327382052887721 2214 6 -0.8446206101400051 2214 7 -0.6777546995583279 2214 8 -0.4382320107289033 2214 9 -0.2463955994603424 2214 10 -0.2679562602101271 2214 11 -0.877400709490594 2214 12 -0.5895845200805994 2214 13 -5.272696712865049 2214 14 -3.961211485974303 2214 15 -2.3995994533393414 2214 16 -3.0404841378110135 2214 17 -3.528302117469863 2214 18 -1.3100534227217437 2214 19 -0.8293631096997771 2214 20 -0.47220283177178146 2214 21 -0.500155438215458 2214 22 -0.42861452889671625 2214 23 -0.29509235987627136 2214 24 -0.30844919537663174 2214 25 -0.1523232291036294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2039.0 3 220.0 4 256.0 5 588.0 6 87.0 7 99.0 8 215.0 9 508.0 10 1259.0 11 2936.0 12 6277.0 13 10625.0 14 16305.0 15 22471.0 16 27276.0 17 30297.0 18 32534.0 19 33339.0 20 34310.0 21 35100.0 22 35930.0 23 38383.0 24 41245.0 25 45064.0 26 50673.0 27 57784.0 28 67690.0 29 83306.0 30 103403.0 31 130055.0 32 169778.0 33 226834.0 34 304140.0 35 417474.0 36 586656.0 37 888437.0 38 1756886.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95879453516621 20.667753716746514 12.90049609900744 23.472955649079836 2 14.964513080975147 21.329429987796974 41.10512329317391 22.600933638053966 3 18.180426235426342 27.279930811445784 29.616261820931822 24.923381132196056 4 11.702289729340293 17.806706743127286 39.56171260776643 30.929290919765997 5 12.451993712324585 38.963598619602294 35.50454030645285 13.079867361620265 6 29.62933019890662 39.442700806503616 18.32452615986375 12.603442834726014 7 25.806973182656616 33.25134870421487 23.759754221114637 17.181923892013877 8 23.73154036629574 37.607607885595634 21.59595015066721 17.064901597441416 9 24.916401860758857 16.993111083423134 21.869623235327406 36.2208638204906 10 14.404782806609933 30.35978525382457 34.179951025271656 21.055480914293845 11 32.05359238576961 25.980985727692886 23.82134679419947 18.144075092338028 12 20.868261343114888 27.64147056574531 32.03276546904251 19.457502622097287 13 26.815527904824677 24.07135296148458 27.024249392980543 22.0888697407102 14 20.29009353388882 23.435991335199496 28.738312068253187 27.5356030626585 15 22.508402639614882 30.769344903222933 24.70650919472952 22.015743262432665 16 21.207384877587625 31.138546344748093 26.159270186010502 21.49479859165378 17 19.97444202738104 30.086846888798092 28.53971072848972 21.399000355331147 18 20.328087869311588 29.717038871056694 29.51166984549541 20.44320341413631 19 20.95145462631929 28.99347399394386 29.637057731165683 20.41801364857117 20 22.10127676880348 28.434845910263434 28.926542946975125 20.537334373957957 21 22.39047683539132 28.61799832764087 28.315900878863527 20.675623958104282 22 21.339766709276844 28.66890750547845 29.14197559045532 20.849350194789384 23 21.415742280486104 28.758482496297322 28.803983715433247 21.021791507783334 24 21.30182197398365 28.33458029653565 28.972424326644624 21.391173402836074 25 21.54936514105345 28.58232050946225 28.509615192557806 21.358699156926495 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4033.0 1 4033.0 2 3907.0 3 3781.0 4 3781.0 5 3781.0 6 6790.5 7 9800.0 8 9800.0 9 9800.0 10 16431.0 11 23062.0 12 23062.0 13 23062.0 14 38886.5 15 54711.0 16 54711.0 17 54711.0 18 86207.5 19 117704.0 20 117704.0 21 117704.0 22 166422.0 23 215140.0 24 215140.0 25 215140.0 26 276955.5 27 338771.0 28 338771.0 29 338771.0 30 398417.5 31 458064.0 32 458064.0 33 458064.0 34 504711.5 35 551359.0 36 551359.0 37 551359.0 38 574643.5 39 597928.0 40 597928.0 41 597928.0 42 598800.5 43 599673.0 44 599673.0 45 599673.0 46 604848.5 47 610024.0 48 610024.0 49 610024.0 50 575888.0 51 541752.0 52 541752.0 53 541752.0 54 487583.0 55 433414.0 56 433414.0 57 433414.0 58 373610.0 59 313806.0 60 313806.0 61 313806.0 62 260437.5 63 207069.0 64 207069.0 65 207069.0 66 156626.0 67 106183.0 68 106183.0 69 106183.0 70 76827.5 71 47472.0 72 47472.0 73 47472.0 74 32873.5 75 18275.0 76 18275.0 77 18275.0 78 12160.0 79 6045.0 80 6045.0 81 6045.0 82 3886.5 83 1728.0 84 1728.0 85 1728.0 86 1105.5 87 483.0 88 483.0 89 483.0 90 311.0 91 139.0 92 139.0 93 139.0 94 85.0 95 31.0 96 31.0 97 31.0 98 31.5 99 32.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10033306852855034 2 0.052599772758336265 3 0.05630665952663246 4 0.051250085781161754 5 0.04893090534150977 6 0.0682447358881197 7 0.06455685879555835 8 0.060773933324322744 9 0.06622971026022535 10 0.06619169090875565 11 0.060165623700807476 12 0.061534320353716844 13 0.06147729132651228 14 0.06362538468455059 15 0.06653386507198299 16 0.06176243646253506 17 0.06484200393158114 18 0.14249652930845272 19 0.06967046156823362 20 0.06425270398380072 21 0.06149630100224713 22 0.06689504891094518 23 0.06609664253008139 24 0.07048787762483226 25 0.06923323902633202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5260479.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.64877464590798 #Duplication Level Percentage of deduplicated Percentage of total 1 80.39856737129021 56.80060268068132 2 13.408874906435923 18.946411630399236 3 3.615973761402906 7.663923461846103 4 1.2725105268586792 3.5960523778633777 5 0.5323270501429657 1.880412690173567 6 0.24736753949408077 1.0485728131458032 7 0.13706242828818946 0.6778304825987254 8 0.07849864954597893 0.44366667214255767 9 0.04981439788991994 0.3167393553581892 >10 0.22728733017115268 2.789111228103291 >50 0.015504602166936972 0.7768457768518281 >100 0.013070657770942386 1.8156974920749371 >500 0.0017057722382672407 0.8205240093847698 >1k 0.0012996283506253418 1.6767533246515662 >5k 1.0830236254773457E-4 0.5144820997613007 >10k+ 2.7075590636933643E-5 0.23237390496357377 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12148 0.23092954082698552 No Hit GGTATCAACGCAGAGTACTTTTTTT 7494 0.142458509956983 No Hit TATCAACGCAGAGTACTTTTTTTTT 6829 0.12981707559330624 No Hit GTACATGGGAAGCAGTGGTATCAAC 6474 0.12306864070743369 No Hit CCCATGTACTCTGCGTTGATACCAC 6099 0.11594001230686407 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.801935146970457E-5 0.0 4 0.0 0.0 0.0 7.603870293940913E-5 0.0 5 0.0 0.0 0.0 1.140580544091137E-4 0.0 6 0.0 0.0 0.0 1.140580544091137E-4 0.0 7 0.0 0.0 0.0 1.33067730143966E-4 0.0 8 0.0 0.0 0.0 1.5207740587881827E-4 0.0 9 0.0 0.0 0.0 2.8514513602278423E-4 0.0 10 0.0 0.0 0.0 2.8514513602278423E-4 0.0 11 0.0 0.0 0.0 3.0415481175763653E-4 0.0 12 0.0 0.0 0.0 3.421741632273411E-4 0.0 13 0.0 0.0 0.0 3.611838389621934E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCCTA 800 0.0 11.405209 16 TGTCCCG 785 0.0 11.371792 11 AGCGTCG 255 0.0 10.809064 17 ATCGCCG 115 8.857969E-7 10.736474 19 ATACCGC 80 3.7835282E-4 10.684552 6 GTCCCGC 835 0.0 10.58517 12 CGTCGGT 270 0.0 10.552945 19 TGCGCTA 120 1.5319838E-6 10.288436 10 TCCCGCC 890 0.0 10.2516775 13 CCGCCCT 910 0.0 10.2352495 15 CCCGCCC 905 0.0 10.08176 14 GTCTTAC 495 0.0 9.980369 1 TATACTG 1285 0.0 9.903788 5 CCGTACA 655 0.0 9.86729 17 GCGTCGG 290 0.0 9.832639 18 CGGGGGA 485 0.0 9.602507 17 TCGCGTT 80 0.0045301863 9.497018 10 GCGCTAT 110 6.8694215E-5 9.496656 11 CCCGTAC 710 0.0 9.236613 16 CTAGTAC 385 0.0 9.127265 3 >>END_MODULE