FastQCFastQC Report
Wed 25 May 2016
SRR1294651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5260479
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT292740.5564892474620657No Hit
GGTATCAACGCAGAGTACTTTTTTT193990.36876869958039943No Hit
TATCAACGCAGAGTACTTTTTTTTT173030.32892441924014904No Hit
ACGCAGAGTACTTTTTTTTTTTTTT104170.19802379212995624No Hit
CCCATGTACTCTGCGTTGATACCAC68690.1305774626227003No Hit
GTACATGGGAAGCAGTGGTATCAAC61700.11728969928403857No Hit
GAGTACTTTTTTTTTTTTTTTTTTT54000.10265224896820233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG1001.0359145E-813.27520319
GCTATCG1657.2759576E-1211.49368113
TACTCCG1001.8845967E-611.4236985
TACACCG1251.780063E-811.4236985
GGTATCA65500.011.3422611
GCGAACT600.00596103811.06266813
TCTATAC7500.010.6425183
TGTCCCG8050.010.13669711
CTACGAC750.002644171210.1357323
AAATCGC1502.5218469E-810.11434317
ACCGGTT856.473727E-410.0797348
CCGTACA6700.010.04829917
CCCCGTA6550.09.98887915
AGGACGG1054.0165614E-59.9730695
CGCCCTA7800.09.96846416
AATCGCC1356.620012E-79.83338918
CCCGTAC6950.09.4139816
GTATCAA123700.09.2970791
GTCTAGA5950.09.2725881
CGCTATC1951.7444108E-99.2392412
Produced by FastQC (version 0.11.5)