##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294651_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5260479 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.315624679805776 31.0 31.0 34.0 28.0 34.0 2 31.4784843737614 31.0 31.0 34.0 28.0 34.0 3 31.627884837103238 31.0 31.0 34.0 28.0 34.0 4 35.21362484290879 37.0 35.0 37.0 32.0 37.0 5 35.309126032059055 37.0 35.0 37.0 32.0 37.0 6 34.93493444228178 37.0 35.0 37.0 32.0 37.0 7 34.771147456343805 37.0 35.0 37.0 32.0 37.0 8 34.82447777094063 37.0 35.0 37.0 32.0 37.0 9 36.284811136020124 39.0 37.0 39.0 32.0 39.0 10 36.055809366409406 39.0 35.0 39.0 30.0 39.0 11 36.20794532208949 39.0 37.0 39.0 31.0 39.0 12 36.1447845338799 39.0 35.0 39.0 31.0 39.0 13 36.002645386475265 39.0 35.0 39.0 30.0 39.0 14 37.10494443566831 40.0 36.0 41.0 31.0 41.0 15 37.15593637005299 40.0 37.0 41.0 31.0 41.0 16 37.20040019169357 40.0 37.0 41.0 31.0 41.0 17 37.2379416399153 40.0 37.0 41.0 31.0 41.0 18 37.237203684303275 40.0 37.0 41.0 31.0 41.0 19 37.25741153989969 40.0 37.0 41.0 31.0 41.0 20 37.30817535817556 40.0 37.0 41.0 31.0 41.0 21 37.157756356407845 40.0 37.0 41.0 30.0 41.0 22 37.14686438250205 40.0 37.0 41.0 30.0 41.0 23 37.15676234046367 40.0 37.0 41.0 30.0 41.0 24 37.07689147699288 40.0 36.0 41.0 30.0 41.0 25 35.984574028334684 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4941523041004956 1101 2 0.5063645719964285 1101 3 0.4402526776544917 1101 4 0.3796923434758668 1101 5 0.38409995527502616 1101 6 0.44807310139984224 1101 7 0.5071105929175843 1101 8 0.47030442364003733 1101 9 0.6984420807016178 1101 10 0.6975526984248077 1101 11 0.7001376204039715 1101 12 0.8212034995353648 1101 13 0.7474759551363803 1101 14 0.9596279528848299 1101 15 0.9002786860172804 1101 16 0.9856230585311536 1101 17 0.9530404246260744 1101 18 0.9301782443620326 1101 19 1.0431070224830634 1101 20 0.9183430894412723 1101 21 1.2364482673008297 1101 22 1.10955185278371 1101 23 1.1233554310236116 1101 24 1.2039270014651606 1101 25 1.141508985784185 1102 1 0.36879372561528356 1102 2 0.39257844155133625 1102 3 0.3324536402078522 1102 4 0.310067888371627 1102 5 0.35372789961536455 1102 6 0.34183652802001774 1102 7 0.3991192906315746 1102 8 0.39447959598054183 1102 9 0.49785398581660445 1102 10 0.6421183717958314 1102 11 0.5922004012775659 1102 12 0.6428071445182795 1102 13 0.5875909889066335 1102 14 0.7582004588145494 1102 15 0.7565383755862882 1102 16 0.749367674996698 1102 17 0.6887665544843387 1102 18 0.6765744978740642 1102 19 0.809418606509027 1102 20 0.7611004265514367 1102 21 1.1062608636017686 1102 22 0.9946040446187396 1102 23 0.9040670924471783 1102 24 0.9810186597325483 1102 25 0.9302789190366809 1103 1 0.29908304518226103 1103 2 0.29190485421950996 1103 3 0.2535548245671535 1103 4 0.21604723844640716 1103 5 0.25587362387040713 1103 6 0.27544494943397524 1103 7 0.29865394434328607 1103 8 0.31393143721460604 1103 9 0.26912789944530857 1103 10 0.49184642507857745 1103 11 0.4867187450436887 1103 12 0.47712885787664305 1103 13 0.6326184588558377 1103 14 0.809303625955387 1103 15 0.7439939613777398 1103 16 0.6626126556890668 1103 17 0.6373719360904317 1103 18 0.678200476701015 1103 19 0.8040531448001218 1103 20 0.6666077198371596 1103 21 0.9547038217002211 1103 22 0.8228395884857207 1103 23 0.8270645271745778 1103 24 0.9587729774254825 1103 25 0.8513833263956272 1104 1 0.18018941276496747 1104 2 0.20367345825394878 1104 3 0.12356873284136682 1104 4 0.13619201556448246 1104 5 0.1418551065231739 1104 6 0.1816520340412353 1104 7 0.19123142536442117 1104 8 0.10788404751173175 1104 9 -0.1013263446124455 1104 10 0.37788331864263114 1104 11 0.41245487600032504 1104 12 0.43337668876107216 1104 13 -0.04910775031553527 1104 14 0.3729470929141101 1104 15 0.4735299454131976 1104 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2207 2 0.27198749448014325 2207 3 0.21596768861082793 2207 4 0.21968279445555083 2207 5 0.19808616186195138 2207 6 0.30433228564067605 2207 7 0.36511597006934693 2207 8 0.3158334790813413 2207 9 0.4242034553461238 2207 10 0.6275702941783408 2207 11 0.5339022137417615 2207 12 0.41019199408827944 2207 13 0.5181893861011062 2207 14 0.5623252489407164 2207 15 0.567114575828839 2207 16 0.6257156477661425 2207 17 0.6980176917413559 2207 18 0.6428179182103193 2207 19 0.7018642338928487 2207 20 0.6751512991812731 2207 21 0.9814199224240596 2207 22 0.8611275274746575 2207 23 0.7715875671895347 2207 24 0.956391961050187 2207 25 0.8612979058124353 2208 1 0.16319497751941014 2208 2 0.1775430725868965 2208 3 0.19890024115487392 2208 4 0.13235829865013216 2208 5 0.14081680303547728 2208 6 0.2569075619972736 2208 7 0.24624536621987403 2208 8 0.1450521294103453 2208 9 0.31318163526859877 2208 10 0.35474171677868327 2208 11 0.039845415331051015 2208 12 -0.13876402040702374 2208 13 0.07508190560418626 2208 14 -0.30941287899075576 2208 15 0.19624253694991012 2208 16 0.308007712282091 2208 17 0.26766301977809803 2208 18 0.3486462035748801 2208 19 0.43929892686492167 2208 20 0.41144249699313207 2208 21 0.669303372250738 2208 22 0.69638988452283 2208 23 0.6840938407427615 2208 24 0.7849979860897847 2208 25 0.7288066119957222 2209 1 -0.021714789290808056 2209 2 0.007360999003047652 2209 3 0.09742077253692116 2209 4 -0.5173044547508425 2209 5 -0.07015531118693019 2209 6 0.09704672841348128 2209 7 0.11704851617318468 2209 8 0.16647788217686355 2209 9 0.2161138153554063 2209 10 0.33486706235196806 2209 11 0.34003468565334316 2209 12 0.28186971169538566 2209 13 0.34004349979210247 2209 14 0.35346763280268334 2209 15 -0.302692703264988 2209 16 -0.2492250342215172 2209 17 0.14790260321803572 2209 18 -0.25644083295972564 2209 19 0.2450032715241548 2209 20 0.289944179840802 2209 21 0.567615257743995 2209 22 0.5885132589820756 2209 23 0.43924469202494976 2209 24 0.5854970096183223 2209 25 0.5860054843280054 2210 1 -0.12311604443969415 2210 2 -0.1303792814867748 2210 3 -0.04122910715990358 2210 4 -0.009177420978573991 2210 5 -0.005251893667448826 2210 6 -0.38388775034262324 2210 7 -0.012542962937530433 2210 8 -0.2670104087102416 2210 9 0.0225324369704083 2210 10 0.17586135067097075 2210 11 0.12054276436389699 2210 12 0.12044412861722975 2210 13 0.16387997819269629 2210 14 0.13023700990049747 2210 15 0.025621749862864363 2210 16 0.12235256791382909 2210 17 -0.8307178442412351 2210 18 -1.3930171069669157 2210 19 -0.21286773695716477 2210 20 -0.06956991886001163 2210 21 -0.2718981120678521 2210 22 -0.2613407842175093 2210 23 -0.13374825467953855 2210 24 0.2092987137238822 2210 25 0.22946998500221838 2211 1 -0.21680701472949693 2211 2 -0.18209785823584212 2211 3 -0.17418848009386778 2211 4 -0.073912437607909 2211 5 -0.08108117446236918 2211 6 -0.05240209654841266 2211 7 -0.14769222973132656 2211 8 -0.05903160030536014 2211 9 -0.02112076467620483 2211 10 -0.007417836180295012 2211 11 -0.33159064196773613 2211 12 -0.18909446541452013 2211 13 -0.08756119927372907 2211 14 -0.16427217804324812 2211 15 -0.17751418607436875 2211 16 -0.21139027410890776 2211 17 -0.3691535433200883 2211 18 -0.3478711488129136 2211 19 -0.2542088832535896 2211 20 -0.27527366913241025 2211 21 0.03688638486622153 2211 22 -0.04114584513401098 2211 23 -0.7006429460321968 2211 24 -0.12116613442658775 2211 25 -0.7421079373381119 2212 1 -0.16844798631342783 2212 2 -0.31211019070909884 2212 3 -0.1929683192704168 2212 4 -0.0712727800898989 2212 5 -0.1072693848623345 2212 6 -0.20159232060894539 2212 7 -0.2168717668518525 2212 8 -0.44053147400894943 2212 9 -0.2406715604824683 2212 10 -0.5397776988886704 2212 11 -0.24074824975398457 2212 12 -0.339529462476861 2212 13 -0.34565961827037484 2212 14 -0.3397726735778832 2212 15 -0.20779352541151752 2212 16 -0.361740008941986 2212 17 -0.6130841987436213 2212 18 -0.44151643266600615 2212 19 -0.41850781040756857 2212 20 -0.6025809948936995 2212 21 -0.13748792715159652 2212 22 -0.49220664474097475 2212 23 -0.309421650693416 2212 24 -0.524139731047093 2212 25 -0.24254112223092505 2213 1 -0.22155256301498483 2213 2 -0.20929871322204363 2213 3 -0.14100100107883407 2213 4 -0.04105169048953172 2213 5 -0.07831311234755844 2213 6 -0.24788888375616835 2213 7 -0.32266712813888887 2213 8 -0.31186470804844646 2213 9 -0.2252481345957449 2213 10 -0.2504160509928326 2213 11 0.00864014458178275 2213 12 0.05561494705106895 2213 13 0.08064015871987351 2213 14 -0.6744970515743987 2213 15 -0.21900668039469906 2213 16 -0.33938872795533825 2213 17 -0.24169211459166462 2213 18 -0.218048869836295 2213 19 -0.30288692165273545 2213 20 -0.36475873208132015 2213 21 -0.09085674903028007 2213 22 -0.40728650141607403 2213 23 -0.4995468231964537 2213 24 -0.6564015157950394 2213 25 -0.4883534800180058 2214 1 -0.24967211866330885 2214 2 -0.13072395717108165 2214 3 -0.16167217864949635 2214 4 -0.12939736926288958 2214 5 -0.057762583714165316 2214 6 -0.035807851603884444 2214 7 -0.06007309603204902 2214 8 -0.0992979029602381 2214 9 -0.10693205205453182 2214 10 -0.044675210969870705 2214 11 0.049136652621960764 2214 12 -0.14585962535428365 2214 13 -0.07455702465136227 2214 14 -0.15430442988603943 2214 15 -0.018101897529120947 2214 16 -0.22958117289952895 2214 17 -0.2991640585587163 2214 18 -0.21627414795587185 2214 19 -0.35117235591316387 2214 20 -0.3596961057302792 2214 21 -0.471852016494708 2214 22 -0.5814686177675981 2214 23 -0.8436946974690613 2214 24 -0.545833750396838 2214 25 -0.8298243955023139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 24.0 9 75.0 10 179.0 11 373.0 12 938.0 13 2191.0 14 4503.0 15 7967.0 16 11449.0 17 14981.0 18 17488.0 19 19519.0 20 21389.0 21 23283.0 22 25578.0 23 28283.0 24 31624.0 25 36013.0 26 41337.0 27 46672.0 28 55377.0 29 69298.0 30 87706.0 31 115469.0 32 155375.0 33 210185.0 34 289141.0 35 402703.0 36 575665.0 37 914545.0 38 2051147.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.37355795613949 21.10011668025272 12.051993116397876 25.47433224720991 2 14.70406021961118 21.756345762429618 39.607685916054415 23.93190810190479 3 17.64091619606407 27.005520840392055 30.037053054431023 25.31650990911285 4 11.674810745697505 17.96363549254409 39.29274462751248 31.068809134245935 5 12.6163081654911 38.4269287342964 35.447618163924346 13.509144936288148 6 29.62428438017132 39.81525068748174 17.775319709432495 12.785145222914435 7 25.642734449243115 33.51381907111291 23.567544586511655 17.275901893132314 8 23.96891451987315 37.4764732243951 21.196182174010012 17.35843008172174 9 24.806329165899943 16.328895962187424 21.968694063954715 36.896080807957915 10 14.201949673352907 30.25346516893384 34.1604258481995 21.384159309513745 11 32.29508605224519 25.47478199470164 23.692886016750307 18.537245936302867 12 20.995781886921723 27.355420462553386 32.143736352059314 19.505061298465577 13 27.09691535577477 23.532922964970666 27.149363071067512 22.220798608187053 14 20.370787531425695 22.988807938522076 29.04358488144969 27.596819648602537 15 22.563811566018618 30.279179759752566 25.01654813978378 22.140460534445033 16 21.02804404545217 30.85042700516588 26.476677486348667 21.644851463033284 17 19.79451673298828 29.81976729486699 28.8102580811588 21.57545789098593 18 20.030335744338757 29.268708578962947 30.113710566431344 20.587245110266952 19 20.73176643286533 28.578149887965836 30.4163579952966 20.273725683872232 20 21.79352795472648 27.954878556478796 29.81315357753205 20.43843991126268 21 22.06809775633988 28.245477065059056 29.05838968289317 20.62803549570789 22 20.99190813684119 28.290819653307974 29.939988738383366 20.777283471467467 23 21.071051975744385 28.377923430079388 29.679441373427828 20.8715832207484 24 20.974040576913243 28.007069318212274 29.79510040815675 21.22378969671773 25 21.233932028194886 28.17758465299226 29.342137755264012 21.24634556354884 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1920.0 1 1920.0 2 2432.0 3 2944.0 4 2944.0 5 2944.0 6 7080.0 7 11216.0 8 11216.0 9 11216.0 10 17297.5 11 23379.0 12 23379.0 13 23379.0 14 36147.5 15 48916.0 16 48916.0 17 48916.0 18 80136.5 19 111357.0 20 111357.0 21 111357.0 22 161310.0 23 211263.0 24 211263.0 25 211263.0 26 275424.0 27 339585.0 28 339585.0 29 339585.0 30 402079.5 31 464574.0 32 464574.0 33 464574.0 34 509904.0 35 555234.0 36 555234.0 37 555234.0 38 574832.0 39 594430.0 40 594430.0 41 594430.0 42 596897.0 43 599364.0 44 599364.0 45 599364.0 46 604760.0 47 610156.0 48 610156.0 49 610156.0 50 577427.5 51 544699.0 52 544699.0 53 544699.0 54 490348.5 55 435998.0 56 435998.0 57 435998.0 58 376160.0 59 316322.0 60 316322.0 61 316322.0 62 262807.0 63 209292.0 64 209292.0 65 209292.0 66 158094.5 67 106897.0 68 106897.0 69 106897.0 70 77316.5 71 47736.0 72 47736.0 73 47736.0 74 32726.0 75 17716.0 76 17716.0 77 17716.0 78 11625.0 79 5534.0 80 5534.0 81 5534.0 82 3521.0 83 1508.0 84 1508.0 85 1508.0 86 934.0 87 360.0 88 360.0 89 360.0 90 212.5 91 65.0 92 65.0 93 65.0 94 38.0 95 11.0 96 11.0 97 11.0 98 7.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09677825916613297 2 0.0 3 0.1317560625182612 4 0.011881047334282677 5 0.02233636898845143 6 0.013363802041601154 7 0.021918156122284684 8 0.01988412081865549 9 1.33067730143966E-4 10 0.3823606177308188 11 0.06429072333527042 12 0.0013306773014396599 13 3.801935146970457E-5 14 0.0010265224896820232 15 0.0013116676257048075 16 7.603870293940913E-5 17 3.801935146970457E-5 18 3.421741632273411E-4 19 5.702902720455685E-4 20 1.7108708161367054E-4 21 2.281161088182274E-4 22 0.0025282868727353534 23 1.140580544091137E-4 24 0.0 25 0.00174889016760641 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5260479.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.57317588890668 #Duplication Level Percentage of deduplicated Percentage of total 1 79.20120697917989 54.31078296797006 2 14.285649171467037 19.592246666444467 3 3.7880809699869475 7.792822279090054 4 1.3225184498948597 3.627571611238577 5 0.5620112895523443 1.9269449505012082 6 0.2724352252749142 1.1209049172666363 7 0.149208571469594 0.7162193930861879 8 0.09076402186004068 0.4979181788314512 9 0.05970070037114562 0.36844799645173365 >10 0.23417398591980043 2.775456891083871 >50 0.016540083445968076 0.7948889108786208 >100 0.014290031352143793 1.9326280490057641 >500 0.0017241696516460247 0.7940590239513814 >1k 0.0014738783672699487 1.8407056625149243 >5k 1.1123610318584812E-4 0.4516237304389335 >10k+ 1.1123610318584812E-4 1.4567787712461027 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 29274 0.5564892474620657 No Hit GGTATCAACGCAGAGTACTTTTTTT 19399 0.36876869958039943 No Hit TATCAACGCAGAGTACTTTTTTTTT 17303 0.32892441924014904 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10417 0.19802379212995624 No Hit CCCATGTACTCTGCGTTGATACCAC 6869 0.1305774626227003 No Hit GTACATGGGAAGCAGTGGTATCAAC 6170 0.11728969928403857 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5400 0.10265224896820233 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.9009675734852283E-5 0.0 3.801935146970457E-5 0.0 3 0.0 1.9009675734852283E-5 0.0 3.801935146970457E-5 0.0 4 0.0 1.9009675734852283E-5 0.0 9.504837867426141E-5 0.0 5 0.0 3.801935146970457E-5 0.0 9.504837867426141E-5 0.0 6 0.0 3.801935146970457E-5 0.0 9.504837867426141E-5 0.0 7 0.0 3.801935146970457E-5 0.0 1.140580544091137E-4 0.0 8 0.0 3.801935146970457E-5 0.0 1.33067730143966E-4 0.0 9 0.0 3.801935146970457E-5 0.0 3.421741632273411E-4 0.0 10 0.0 3.801935146970457E-5 0.0 3.421741632273411E-4 0.0 11 1.9009675734852283E-5 3.801935146970457E-5 0.0 3.8019351469704564E-4 0.0 12 1.9009675734852283E-5 3.801935146970457E-5 0.0 3.8019351469704564E-4 0.0 13 1.9009675734852283E-5 3.801935146970457E-5 0.0 4.372225419016025E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 100 1.0359145E-8 13.275203 19 GCTATCG 165 7.2759576E-12 11.493681 13 TACTCCG 100 1.8845967E-6 11.423698 5 TACACCG 125 1.780063E-8 11.423698 5 GGTATCA 6550 0.0 11.342261 1 GCGAACT 60 0.005961038 11.062668 13 TCTATAC 750 0.0 10.642518 3 TGTCCCG 805 0.0 10.136697 11 CTACGAC 75 0.0026441712 10.135732 3 AAATCGC 150 2.5218469E-8 10.114343 17 ACCGGTT 85 6.473727E-4 10.079734 8 CCGTACA 670 0.0 10.048299 17 CCCCGTA 655 0.0 9.988879 15 AGGACGG 105 4.0165614E-5 9.973069 5 CGCCCTA 780 0.0 9.968464 16 AATCGCC 135 6.620012E-7 9.833389 18 CCCGTAC 695 0.0 9.41398 16 GTATCAA 12370 0.0 9.297079 1 GTCTAGA 595 0.0 9.272588 1 CGCTATC 195 1.7444108E-9 9.23924 12 >>END_MODULE