FastQCFastQC Report
Wed 25 May 2016
SRR1294650_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294650_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550245
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC244930.5382787080695655No Hit
CCCATGTACTCTGCGTTGATACCAC227260.4994456342460681No Hit
ATTCCATTCCATTCCATTCCATTCC177700.39052842209595306No Hit
GTATCAACGCAGAGTACTTTTTTTT147540.324246276848829No Hit
GAATGGAATGGAATGGAATGGAATG137210.3015442025649168No Hit
GAGTACATGGGAAGCAGTGGTATCA109640.24095405851772816No Hit
GGTATCAACGCAGAGTACTTTTTTT95620.2101425307868038No Hit
TATCAACGCAGAGTACTTTTTTTTT85310.1874844101801112No Hit
CATGTACTCTGCGTTGATACCACTG82280.18082542808134505No Hit
GCGTTGATACCACTGCTTCCCATGT78560.17265004411850352No Hit
GTATCAACGCAGAGTACATGGGAAG67200.14768435545778305No Hit
GTACTGGTTCACTATCGGTCAGTCA63040.13854199059611075No Hit
GGTATCAACGCAGAGTACATGGGAA59620.13102591179156287No Hit
ACGCAGAGTACATGGGAAGCAGTGG56930.1251141422055296No Hit
TATCAACGCAGAGTACATGGGAAGC54490.11975179358474103No Hit
ACTCTGCGTTGATACCACTGCTTCC46850.10296148888686214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACA8200.010.66350717
AATCGCC2250.010.56233818
ATCGCCG2151.8189894E-1210.16182519
CCCGTAC8650.010.10853616
TGGACCG1252.5830814E-69.8769975
CTGGTCG1356.4784035E-79.8488589
CTTAGAC3000.09.8138993
CCCCGTA8850.09.77248715
GTACTGG17300.09.6639291
CACGACG800.00452920249.4972174
GTCCAGG6750.09.2881091
CGGGATG1554.2720058E-79.19267619
CGCCCTA8850.09.12834716
CTAGGAC2501.8189894E-129.1174293
AGGACGG1252.766555E-59.1172285
TCGCACG1054.509361E-49.0448699
CCCTTAG2950.09.0161921
CTAGACA3900.09.010184
ACCGGGG4350.08.95780515
TTAGACT3950.08.8961284
Produced by FastQC (version 0.11.5)