##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294650_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4550245 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.88486246344977 31.0 30.0 33.0 25.0 34.0 2 30.092873240891425 31.0 30.0 34.0 25.0 34.0 3 30.030708236589458 31.0 30.0 34.0 25.0 34.0 4 33.64833036462872 35.0 35.0 37.0 28.0 37.0 5 34.0199200262843 35.0 35.0 37.0 28.0 37.0 6 34.068180504566236 37.0 35.0 37.0 29.0 37.0 7 33.988496883134864 37.0 35.0 37.0 29.0 37.0 8 33.82787652093459 37.0 35.0 37.0 28.0 37.0 9 34.96362921117434 39.0 35.0 39.0 27.0 39.0 10 35.08178878280181 38.0 35.0 39.0 27.0 39.0 11 35.31370244898901 39.0 35.0 39.0 28.0 39.0 12 35.10190572156005 38.0 35.0 39.0 27.0 39.0 13 34.95146239378319 38.0 35.0 39.0 27.0 39.0 14 35.83544973951952 39.0 35.0 41.0 27.0 41.0 15 35.807174998269325 39.0 34.0 41.0 27.0 41.0 16 35.91263569324289 39.0 35.0 41.0 27.0 41.0 17 35.91446548482554 39.0 35.0 41.0 27.0 41.0 18 35.804846112681844 39.0 34.0 41.0 27.0 41.0 19 36.089568363901286 39.0 35.0 41.0 27.0 41.0 20 36.20495621664328 39.0 35.0 41.0 27.0 41.0 21 36.25276001621891 39.0 35.0 41.0 27.0 41.0 22 36.26622434616158 39.0 35.0 41.0 27.0 41.0 23 36.25928296168668 39.0 35.0 41.0 27.0 41.0 24 36.273026617248085 39.0 35.0 41.0 27.0 41.0 25 35.06091935708956 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9754630055059081 1101 2 0.9441854752825698 1101 3 1.2706896917919082 1101 4 1.0538474754019589 1101 5 0.7487499193402058 1101 6 0.75225505585761 1101 7 0.7591972416331387 1101 8 0.908253840444921 1101 9 1.1525057747080183 1101 10 1.1247459971450198 1101 11 0.8937321933764864 1101 12 1.0996259805291544 1101 13 1.1394685059705694 1101 14 1.232132764223131 1101 15 1.3659224368606075 1101 16 1.2433904548229364 1101 17 1.2130327251414812 1101 18 1.2477964174606981 1101 19 1.138194187833129 1101 20 1.0434883478191352 1101 21 0.9368282688097835 1101 22 0.9324423358549154 1101 23 0.9526997326795268 1101 24 1.0191650418704512 1101 25 1.0446229295690586 1102 1 0.9246980167186045 1102 2 0.8816578697307413 1102 3 1.1603177255336838 1102 4 1.0083995216969655 1102 5 0.8911624016233333 1102 6 0.7740824281341148 1102 7 0.6461298630920922 1102 8 0.7098617533521008 1102 9 1.122791033679988 1102 10 1.0310945924881736 1102 11 0.7831248357477705 1102 12 0.9927921682562584 1102 13 1.0948131325088184 1102 14 1.3149787192573186 1102 15 1.4261396700747113 1102 16 1.202708741885381 1102 17 1.2843010014545726 1102 18 1.347351020583858 1102 19 1.0893735957116562 1102 20 0.34737539657744065 1102 21 0.6540937940036429 1102 22 0.6887373369756205 1102 23 0.8645112837319431 1102 24 0.9726907748914257 1102 25 1.0121195643002991 1103 1 0.5797742582431233 1103 2 0.6328386533288786 1103 3 0.8766311192258449 1103 4 0.7063365234410597 1103 5 0.43478270321148216 1103 6 0.3290180133647169 1103 7 0.22698515757803506 1103 8 0.39212575800162597 1103 9 0.48386353039270347 1103 10 0.43725013507018673 1103 11 0.44831475316735236 1103 12 0.6685260444009486 1103 13 0.5512746873061829 1103 14 0.4617811681929851 1103 15 0.7956554591790379 1103 16 0.7742958824724155 1103 17 0.784149190067815 1103 18 0.8718056550339526 1103 19 0.7605490845685452 1103 20 -0.19872251397678298 1103 21 0.21081858018787614 1103 22 -0.3229862073535017 1103 23 0.24325123481974487 1103 24 0.375171853457978 1103 25 0.33059802796644533 1104 1 0.360102803731138 1104 2 0.41778146411242645 1104 3 0.7423118332427805 1104 4 0.5243519832499217 1104 5 0.4026856420042719 1104 6 0.25815685621283535 1104 7 0.2746214745273079 1104 8 0.3039338728447305 1104 9 0.6848155557605438 1104 10 0.6306060777698193 1104 11 0.383505844586729 1104 12 0.48465319137318374 1104 13 0.49956388418129194 1104 14 0.8462208780933054 1104 15 0.8371972446852141 1104 16 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0.32523601207923747 2207 8 0.43529386268339465 2207 9 -1.2677622449165042 2207 10 -0.19143057230372307 2207 11 0.15105205833621937 2207 12 0.5286513167827991 2207 13 0.5831279909517164 2207 14 0.8580028657802572 2207 15 0.8134181572778587 2207 16 0.8097015623531618 2207 17 0.7622061744405286 2207 18 0.8514561091340838 2207 19 -0.43296276140556955 2207 20 0.2600034786437817 2207 21 -0.286127143662128 2207 22 0.3077819357087819 2207 23 -0.5287945397883931 2207 24 -1.6588196341078643 2207 25 -0.29725852812089215 2208 1 0.4691446082012831 2208 2 0.11663168799076118 2208 3 0.5686053686910633 2208 4 0.41466837316500715 2208 5 0.3532971741881923 2208 6 0.20383776862720282 2208 7 0.35444907286439786 2208 8 0.47940165803638024 2208 9 -0.22530925898983867 2208 10 -0.02178157134137848 2208 11 0.19121977724255856 2208 12 0.1846385208962218 2208 13 0.43093815831707616 2208 14 0.6490860664008551 2208 15 0.6446212707574617 2208 16 0.3824876197574554 2208 17 0.4267214676523565 2208 18 0.6495441057452638 2208 19 -1.3784417824424722 2208 20 -0.20985909340901543 2208 21 -0.3280719404327641 2208 22 0.10816029678139216 2208 23 0.23315140694624148 2208 24 0.3968163750203857 2208 25 0.5246546392615841 2209 1 0.2694827288584847 2209 2 -1.3354311010810918 2209 3 -0.1717565314506757 2209 4 0.14656109644857906 2209 5 0.16102521273133164 2209 6 0.166241797201792 2209 7 0.2375614101954895 2209 8 0.3589330786924805 2209 9 -1.9521345287047467 2209 10 -0.3502887528424381 2209 11 -0.023310035626593617 2209 12 0.28967187713247 2209 13 0.4857973197756422 2209 14 0.6503454321740776 2209 15 0.8112376390673006 2209 16 0.7536281069618127 2209 17 0.5052457142142543 2209 18 0.682651354298045 2209 19 0.43034214100431 2209 20 0.37263252070473385 2209 21 0.4520437113201723 2209 22 0.4211710563359219 2209 23 0.37957140895645836 2209 24 0.392298106669287 2209 25 0.45626666996025733 2210 1 0.18071650999394606 2210 2 0.24016016290385522 2210 3 0.18179353262580022 2210 4 0.3259863706024859 2210 5 0.2454416386780025 2210 6 0.19061171106220343 2210 7 0.1830228369769813 2210 8 0.3020016631787712 2210 9 0.3809423360494435 2210 10 -1.5315717002198568 2210 11 -0.3555711565459916 2210 12 -2.4256995012051377 2210 13 -0.6991995830806985 2210 14 -0.3595965003313424 2210 15 -0.6259626397298561 2210 16 -0.053073574850607486 2210 17 0.1879015235028234 2210 18 0.43931510511842475 2210 19 0.30853240289813755 2210 20 0.3604009958137837 2210 21 0.3306183165528225 2210 22 0.2354822411667712 2210 23 0.2074273693560258 2210 24 0.15989509050355366 2210 25 0.16861964758203385 2211 1 -0.34757609440313786 2211 2 -0.10967699048172719 2211 3 -1.4625772755061313 2211 4 -0.5738300141953161 2211 5 -0.4667000351554407 2211 6 -0.35638078562357123 2211 7 -0.37026818991936494 2211 8 -0.05494766514421201 2211 9 0.0874055220361214 2211 10 0.11777536891615625 2211 11 -0.17108524013426774 2211 12 -0.2844636540342904 2211 13 -0.23480539263716338 2211 14 -0.8392793940016148 2211 15 -0.2432926215543958 2211 16 0.018144332202311375 2211 17 -0.22745688392892305 2211 18 -0.09758219583297034 2211 19 -0.057207061051670394 2211 20 -0.08299426968363122 2211 21 -0.13878548671687696 2211 22 -0.1997192501708014 2211 23 -0.4605277734361053 2211 24 -0.317639386896559 2211 25 -0.2298478086145579 2212 1 -0.39968059602924555 2212 2 -0.4364609414049667 2212 3 -2.4501120502850284 2212 4 -1.5065386326850145 2212 5 -0.5909330916249829 2212 6 -0.5017849943726489 2212 7 -0.48651567673068996 2212 8 -0.3957597602857206 2212 9 -1.012912407452255 2212 10 -0.29237845348178837 2212 11 -0.5058356179068753 2212 12 -0.15242565341409886 2212 13 -0.5889008099281554 2212 14 -0.21963866560264478 2212 15 -0.04424581428896346 2212 16 -1.2943160318573774 2212 17 -1.5336872963079173 2212 18 -0.4500098852625882 2212 19 -0.29644320261122914 2212 20 -0.35185831634315434 2212 21 -0.26260887913397113 2212 22 -0.4716857027557353 2212 23 -0.15477906879264935 2212 24 -0.2540797807967934 2212 25 -0.1691113299509297 2213 1 -3.0291384383604765 2213 2 -1.382838618263598 2213 3 -2.5095314606910115 2213 4 -1.1047557730626778 2213 5 -0.8529451073109513 2213 6 -0.6948674925130405 2213 7 -0.37270114502597806 2213 8 -0.1530672907245858 2213 9 -0.3858402480870069 2213 10 -0.18934141789473813 2213 11 -0.5878841895964442 2213 12 -0.2408718519812183 2213 13 -0.6655672808464743 2213 14 -0.2289880959749624 2213 15 -0.2861034171547203 2213 16 -1.7153390779626392 2213 17 -1.0151072711216287 2213 18 -0.3529699265002364 2213 19 -0.27637385875968334 2213 20 -0.16681438715185948 2213 21 -0.01919108038936912 2213 22 -0.07487436174906037 2213 23 0.03368075059687925 2213 24 0.09609622959091979 2213 25 -0.028989280857238953 2214 1 -1.0109627722107533 2214 2 -0.8559232825536753 2214 3 -6.706937622366169 2214 4 -2.3550074631931217 2214 5 -1.5587276024604293 2214 6 -0.8745835103173079 2214 7 -0.7957958124714182 2214 8 -0.5784437517559198 2214 9 -0.3961044253778283 2214 10 -0.3303504610157262 2214 11 -1.0290033345616365 2214 12 -0.7745964496731119 2214 13 -5.243219291883516 2214 14 -3.9233851205397556 2214 15 -2.3980376514433885 2214 16 -3.0640531018493675 2214 17 -3.7119019647498206 2214 18 -1.2991314673889747 2214 19 -0.9650708302876154 2214 20 -0.6239231395771228 2214 21 -0.6780376964360428 2214 22 -0.6001988600807522 2214 23 -0.5227108628650896 2214 24 -0.4970826926194363 2214 25 -0.24453646688236574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1790.0 3 232.0 4 267.0 5 513.0 6 72.0 7 73.0 8 133.0 9 358.0 10 1106.0 11 2702.0 12 5767.0 13 10577.0 14 16796.0 15 23808.0 16 30380.0 17 34208.0 18 35213.0 19 35409.0 20 35302.0 21 34907.0 22 35158.0 23 36368.0 24 38076.0 25 40929.0 26 45373.0 27 51701.0 28 60245.0 29 73615.0 30 91602.0 31 114756.0 32 149738.0 33 198488.0 34 267767.0 35 365420.0 36 510333.0 37 762414.0 38 1438649.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98934513218266 21.762994310011948 12.596204445336163 21.651456112469223 2 14.267161858773497 22.82134163637683 40.98222629751546 21.92927020733421 3 17.632160169302935 30.47463842370945 29.59318214402888 22.300019262958738 4 11.720628833597015 17.70110064569895 38.39535070369421 32.18291981700982 5 13.872537756146471 38.2044598262728 33.31958350328226 14.603418914298466 6 30.173098775757595 39.06577375212143 17.55122049272749 13.209906979393486 7 23.915375052477152 34.52432553801589 25.147777200868138 16.412522208638823 8 23.228646793706712 38.04294116288812 22.493894842860236 16.23451720054493 9 24.42009063733126 16.894579507571034 22.45342284907875 36.23190700601896 10 16.258398962512473 30.05663561197491 31.364754849148003 22.32021057636461 11 31.777159502081844 28.585961163861484 21.669524701291767 17.967354632764906 12 20.05127362713412 29.454121472931476 32.35267793428593 18.141926965648473 13 25.589881240879315 26.314280210302428 27.112480307237874 20.983358241580383 14 19.85849416557278 23.388025201259754 28.659472694713468 28.094007938454002 15 24.315554877225424 29.43673723596989 23.6753026205193 22.572405266285383 16 22.145273780634632 32.11221053163989 25.300565494810705 20.441950192914767 17 19.774519592362736 30.94860990767905 29.512577025761022 19.764293474197196 18 20.140764536439697 30.615989555455393 29.709178768628338 19.534067139476573 19 20.815417560393502 27.801496864616027 30.26689635803703 21.11618921695344 20 23.296149646654182 27.24526608968727 27.90379669448204 21.5547875691765 21 23.64546221623389 29.65714415145657 27.049720904181168 19.647672728128367 22 20.9438061894122 28.947509632470663 29.498495751156778 20.610188426960363 23 20.409866511668106 30.196547763329868 29.224551965532612 20.169033759469414 24 20.862770887448505 27.319289231804657 29.118292009686296 22.699647871060545 25 22.61705152351939 27.239072508033917 27.246242114532482 22.897633853914208 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5950.0 1 5950.0 2 6036.5 3 6123.0 4 6123.0 5 6123.0 6 8975.0 7 11827.0 8 11827.0 9 11827.0 10 16707.0 11 21587.0 12 21587.0 13 21587.0 14 32340.5 15 43094.0 16 43094.0 17 43094.0 18 66236.0 19 89378.0 20 89378.0 21 89378.0 22 124067.0 23 158756.0 24 158756.0 25 158756.0 26 206778.5 27 254801.0 28 254801.0 29 254801.0 30 310471.0 31 366141.0 32 366141.0 33 366141.0 34 428313.5 35 490486.0 36 490486.0 37 490486.0 38 546457.5 39 602429.0 40 602429.0 41 602429.0 42 589008.5 43 575588.0 44 575588.0 45 575588.0 46 590017.0 47 604446.0 48 604446.0 49 604446.0 50 538064.0 51 471682.0 52 471682.0 53 471682.0 54 397214.5 55 322747.0 56 322747.0 57 322747.0 58 269101.0 59 215455.0 60 215455.0 61 215455.0 62 184015.5 63 152576.0 64 152576.0 65 152576.0 66 118518.0 67 84460.0 68 84460.0 69 84460.0 70 63697.5 71 42935.0 72 42935.0 73 42935.0 74 30947.5 75 18960.0 76 18960.0 77 18960.0 78 13018.5 79 7077.0 80 7077.0 81 7077.0 82 4830.5 83 2584.0 84 2584.0 85 2584.0 86 1689.0 87 794.0 88 794.0 89 794.0 90 527.0 91 260.0 92 260.0 93 260.0 94 161.5 95 63.0 96 63.0 97 63.0 98 54.5 99 46.0 100 46.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1036647477223754 2 0.05527174910361969 3 0.058392460186209756 4 0.05406302298008129 5 0.05076649718861292 6 0.07006216148800778 7 0.06654586731044153 8 0.06285375842399696 9 0.06839192175366382 10 0.06826006072200508 11 0.06221643010431307 12 0.06324934151897316 13 0.06349108674368084 14 0.0661942378926849 15 0.06880948168724982 16 0.06373283196838853 17 0.06735901033900372 18 0.14568446314429223 19 0.07195216960844966 20 0.06593051582936743 21 0.06333724887341231 22 0.06867762065559108 23 0.06826006072200508 24 0.07944627157438774 25 0.07193019276983986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4550245.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.73065719948554 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3968249847595 41.81248439369965 2 13.999537178855478 15.324077405747843 3 4.656521595262345 7.6456346151691505 4 1.918588203501831 4.20022373091342 5 0.941838584237225 2.5773722345568175 6 0.5339570346308912 1.753429165298228 7 0.33272370836434534 1.274713105724141 8 0.2212588633898773 0.9687714403631342 9 0.16457580244991693 0.8106607644494992 >10 0.7345523468775006 7.096693231535799 >50 0.04864152748906108 1.8318972413294694 >100 0.04119081671770945 4.756570225892311 >500 0.005139410881803579 1.9552986304649165 >1k 0.004038108032285722 4.225366282315667 >5k 3.671007302077713E-4 1.4355494087106544 >10k+ 2.447338201385142E-4 2.3312581238291923 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 24493 0.5382787080695655 No Hit CCCATGTACTCTGCGTTGATACCAC 22726 0.4994456342460681 No Hit ATTCCATTCCATTCCATTCCATTCC 17770 0.39052842209595306 No Hit GTATCAACGCAGAGTACTTTTTTTT 14754 0.324246276848829 No Hit GAATGGAATGGAATGGAATGGAATG 13721 0.3015442025649168 No Hit GAGTACATGGGAAGCAGTGGTATCA 10964 0.24095405851772816 No Hit GGTATCAACGCAGAGTACTTTTTTT 9562 0.2101425307868038 No Hit TATCAACGCAGAGTACTTTTTTTTT 8531 0.1874844101801112 No Hit CATGTACTCTGCGTTGATACCACTG 8228 0.18082542808134505 No Hit GCGTTGATACCACTGCTTCCCATGT 7856 0.17265004411850352 No Hit GTATCAACGCAGAGTACATGGGAAG 6720 0.14768435545778305 No Hit GTACTGGTTCACTATCGGTCAGTCA 6304 0.13854199059611075 No Hit GGTATCAACGCAGAGTACATGGGAA 5962 0.13102591179156287 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5693 0.1251141422055296 No Hit TATCAACGCAGAGTACATGGGAAGC 5449 0.11975179358474103 No Hit ACTCTGCGTTGATACCACTGCTTCC 4685 0.10296148888686214 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.1976838609789142E-5 0.0 6 0.0 0.0 0.0 2.1976838609789142E-5 0.0 7 0.0 0.0 0.0 2.1976838609789142E-5 0.0 8 0.0 0.0 0.0 2.1976838609789142E-5 0.0 9 0.0 0.0 0.0 4.3953677219578285E-5 0.0 10 0.0 0.0 0.0 6.593051582936743E-5 0.0 11 0.0 0.0 0.0 8.790735443915657E-5 0.0 12 0.0 0.0 0.0 1.0988419304894572E-4 0.0 13 0.0 0.0 0.0 1.3186103165873487E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTACA 820 0.0 10.663507 17 AATCGCC 225 0.0 10.562338 18 ATCGCCG 215 1.8189894E-12 10.161825 19 CCCGTAC 865 0.0 10.108536 16 TGGACCG 125 2.5830814E-6 9.876997 5 CTGGTCG 135 6.4784035E-7 9.848858 9 CTTAGAC 300 0.0 9.813899 3 CCCCGTA 885 0.0 9.772487 15 GTACTGG 1730 0.0 9.663929 1 CACGACG 80 0.0045292024 9.497217 4 GTCCAGG 675 0.0 9.288109 1 CGGGATG 155 4.2720058E-7 9.192676 19 CGCCCTA 885 0.0 9.128347 16 CTAGGAC 250 1.8189894E-12 9.117429 3 AGGACGG 125 2.766555E-5 9.117228 5 TCGCACG 105 4.509361E-4 9.044869 9 CCCTTAG 295 0.0 9.016192 1 CTAGACA 390 0.0 9.01018 4 ACCGGGG 435 0.0 8.957805 15 TTAGACT 395 0.0 8.896128 4 >>END_MODULE