FastQCFastQC Report
Wed 25 May 2016
SRR1294650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550245
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT375550.8253401739906312No Hit
GGTATCAACGCAGAGTACTTTTTTT272560.5990007131484129No Hit
CCCATGTACTCTGCGTTGATACCAC266440.5855508879192219No Hit
TATCAACGCAGAGTACTTTTTTTTT241830.5314658881005309No Hit
GTACATGGGAAGCAGTGGTATCAAC235620.5178182713238517No Hit
ATTCCATTCCATTCCATTCCATTCC176000.3867923595322889No Hit
GAATGGAATGGAATGGAATGGAATG151930.33389410899852645No Hit
ACGCAGAGTACTTTTTTTTTTTTTT140400.3085548140814396No Hit
GAGTACATGGGAAGCAGTGGTATCA116210.2553928414843596No Hit
CATGTACTCTGCGTTGATACCACTG96690.21249405251805126No Hit
GCGTTGATACCACTGCTTCCCATGT82500.18130891853076042No Hit
GTACTTTTTTTTTTTTTTTTTTTTT80510.1769355276474124No Hit
ACGCAGAGTACATGGGAAGCAGTGG73490.16150778694334042No Hit
GAGTACTTTTTTTTTTTTTTTTTTT70830.1556619478731365No Hit
GTATCAACGCAGAGTACATGGGAAG68620.1508050665403731No Hit
GGTATCAACGCAGAGTACATGGGAA63600.13977269355825894No Hit
GTACTGGTTCACTATCGGTCAGTCA58600.12878427425336436No Hit
TATCAACGCAGAGTACATGGGAAGC58040.1275535712912162No Hit
ACTCTGCGTTGATACCACTGCTTCC55500.12197145428432975No Hit
GCTTCCCATGTACTCTGCGTTGATA46540.10228020688995867No Hit
GTGGTATCAACGCAGAGTACATGGG45610.10023636089924828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC909.3170296E-510.5783898
GGACCGA856.4683583E-410.0805836
GAAATCG2400.09.87724316
TGTCCCG8300.09.60251711
TGGACCG1304.12824E-69.5205515
GGACCGT1502.5700683E-79.520556
GTATTAG4250.09.3984851
CCGTACA9100.09.37795417
CGCCTTT7000.09.21103214
CCCCGTA8650.09.20790115
AAATCGC2800.09.14350417
CCCGTAC8950.09.11134216
ATCGCCG2401.0913936E-119.08706419
CGACTAA7250.09.02439417
CGCGCGA2002.75395E-99.00784713
CGCGCCT6350.08.95941712
TGTATCG7550.08.9531015
AGCGACT7100.08.94775115
CTAGGGT2356.366463E-118.9128564
GTCTAAG3100.08.8967991
Produced by FastQC (version 0.11.5)