FastQCFastQC Report
Wed 25 May 2016
SRR1294649_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294649_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285334
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC125050.29180922653870156No Hit
CCCATGTACTCTGCGTTGATACCAC115720.2700372946426113No Hit
GTATCAACGCAGAGTACTTTTTTTT66610.15543712578762822No Hit
GAGTACATGGGAAGCAGTGGTATCA59900.139779069729454No Hit
GGTATCAACGCAGAGTACTTTTTTT46140.10766955387841413No Hit
ATTCCATTCCATTCCATTCCATTCC45330.10577938615753171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG658.037459E-411.69074819
GCACCGC803.7843606E-410.6842036
CGCCCTA2550.010.43607516
GACTCCG1356.47844E-79.8488137
CGAACCG1002.746535E-49.50372712
TATACCG1151.11481575E-49.0844725
TGCACCG1151.11481575E-49.0844725
TATCGCG1151.1152412E-49.0841537
TAGACAG7950.09.0792775
GTCGCCC2201.6916601E-109.06517311
GTATTAG7150.09.0351311
TCGCCCA2651.8189894E-128.9657812
CGTAGAC850.0074551978.9388363
GTCCCGC3100.08.89058412
CCTACAC4550.08.7669343
CTGTCGC2607.2759576E-128.7664239
GTCCTAG4700.08.6916491
GTACTAG3400.08.6619241
CCGCTAC1000.0029060738.5501581
CTAGGAC3450.08.5339973
Produced by FastQC (version 0.11.5)