##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294649_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4285334 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.03454713214886 31.0 30.0 34.0 25.0 34.0 2 30.24721830317077 31.0 30.0 34.0 25.0 34.0 3 30.14185568732799 31.0 30.0 34.0 25.0 34.0 4 33.70619909673319 35.0 35.0 37.0 28.0 37.0 5 34.081914501880135 35.0 35.0 37.0 28.0 37.0 6 34.19878847249713 37.0 35.0 37.0 30.0 37.0 7 34.071134478666075 37.0 35.0 37.0 29.0 37.0 8 33.897231581015625 37.0 35.0 37.0 28.0 37.0 9 35.04539902840712 38.0 35.0 39.0 27.0 39.0 10 35.148009933414755 38.0 35.0 39.0 27.0 39.0 11 35.40277863989132 39.0 35.0 39.0 28.0 39.0 12 35.16795913690741 39.0 35.0 39.0 27.0 39.0 13 35.04699096966537 38.0 35.0 39.0 27.0 39.0 14 35.93894804932358 39.0 35.0 41.0 27.0 41.0 15 35.93460463058422 39.0 35.0 41.0 27.0 41.0 16 36.035524885574844 39.0 35.0 41.0 27.0 41.0 17 35.997110843635525 39.0 35.0 41.0 27.0 41.0 18 35.88454575536002 39.0 35.0 41.0 27.0 41.0 19 36.15344148204084 39.0 35.0 41.0 27.0 41.0 20 36.26044574355231 39.0 35.0 41.0 27.0 41.0 21 36.28897607514374 39.0 36.0 41.0 27.0 41.0 22 36.281489377490765 39.0 35.0 41.0 27.0 41.0 23 36.24662231695359 39.0 35.0 41.0 27.0 41.0 24 36.24846954753119 39.0 35.0 41.0 27.0 41.0 25 34.95857685771984 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1138541622653158 1101 2 1.0940049142678667 1101 3 1.3962270886514396 1101 4 1.196011290122101 1101 5 0.9440364782204824 1101 6 0.8716231670124586 1101 7 0.8675929027680596 1101 8 1.0763571813182224 1101 9 1.3770099438631576 1101 10 1.327688673534702 1101 11 1.1317066908427904 1101 12 1.2928582264516422 1101 13 1.3448520612056853 1101 14 1.4392492133513315 1101 15 1.5942504701664575 1101 16 1.5469016835932763 1101 17 1.4579319776780792 1101 18 1.530986740460392 1101 19 1.3784124152134467 1101 20 1.3498973301863373 1101 21 1.1704136363110038 1101 22 1.0671780147912457 1101 23 1.1941708412747971 1101 24 1.2396889514581133 1101 25 1.1707789436857965 1102 1 0.9136816182572005 1102 2 0.9152656483492443 1102 3 1.1918584893117803 1102 4 0.9797461983942313 1102 5 0.8533400534873721 1102 6 0.7082453097294916 1102 7 0.6551493847965801 1102 8 0.7264759007574355 1102 9 1.0914435026743519 1102 10 0.9646475648366746 1102 11 0.7535013540765902 1102 12 0.9936108307620728 1102 13 1.0200802503627813 1102 14 1.227025163371124 1102 15 1.386272394648799 1102 16 1.223367896563559 1102 17 1.175478787140868 1102 18 1.3504725264282982 1102 19 1.0036761350461418 1102 20 0.33402745115507315 1102 21 0.6578802639037136 1102 22 0.7001607885800567 1102 23 0.8963646989899985 1102 24 0.864543921700438 1102 25 0.8294286957932613 1103 1 0.6666043017157541 1103 2 0.7002343881583997 1103 3 0.9981597206430273 1103 4 0.8142551651715664 1103 5 0.5894437508646888 1103 6 0.5289874395527718 1103 7 0.5287519956365188 1103 8 0.6550290384148667 1103 9 0.7858251272708756 1103 10 0.7291639375694601 1103 11 0.7147389070252288 1103 12 0.8101212693964825 1103 13 0.7026372272161794 1103 14 0.6158856467212885 1103 15 0.8948930843039733 1103 16 0.8785552010232962 1103 17 0.9690495485804291 1103 18 1.0019106060485896 1103 19 0.8255196199930026 1103 20 -0.035113866375780844 1103 21 0.36355967499753916 1103 22 -0.17376603334623297 1103 23 0.3558881043177422 1103 24 0.5394213982035225 1103 25 0.46697572317283687 1104 1 0.3590663279334443 1104 2 0.34124801746340694 1104 3 0.6775910137472039 1104 4 0.49119528634498266 1104 5 0.3402120860123219 1104 6 0.20233110019935907 1104 7 0.22387321730037257 1104 8 0.45065243007178424 1104 9 0.6907640508034376 1104 10 0.5634585706706048 1104 11 0.3807779233340227 1104 12 0.5667846232528646 1104 13 0.43488508156480776 1104 14 0.7770791005146052 1104 15 0.7476788712075262 1104 16 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0.3110318893458057 2207 6 0.2728780831850912 2207 7 0.3637053072652847 2207 8 0.32014719919983037 2207 9 -1.3160541619413308 2207 10 -0.224841261685917 2207 11 0.060278105301989626 2207 12 0.38944322139452225 2207 13 0.5374026793144182 2207 14 0.7191029146583077 2207 15 0.7519600322342441 2207 16 0.7479464827345765 2207 17 0.7418173603827753 2207 18 0.7361398363102509 2207 19 -0.38191330076008967 2207 20 0.21593943177437325 2207 21 -0.34065282369612504 2207 22 0.21213651142075207 2207 23 -0.568912066257198 2207 24 -1.5326211546926913 2207 25 -0.2450562655160553 2208 1 0.5419891588974011 2208 2 0.21392548973870262 2208 3 0.7486592905816636 2208 4 0.5561788142238626 2208 5 0.41660986366603936 2208 6 0.346006538306149 2208 7 0.42080594310461805 2208 8 0.6059787931046756 2208 9 -0.05885034794220445 2208 10 0.14489245354405256 2208 11 0.3602400916612041 2208 12 0.38209524370694936 2208 13 0.6445429142032708 2208 14 0.8087110606933834 2208 15 0.7087085144314358 2208 16 0.5741209420770375 2208 17 0.6307228870212 2208 18 0.7862606950930129 2208 19 -1.2810569509690097 2208 20 -0.013540586710412583 2208 21 -0.15317914419392054 2208 22 0.2577334696543332 2208 23 0.4145629109894742 2208 24 0.5960049252690567 2208 25 0.6392327678801237 2209 1 0.250823938126409 2209 2 -1.250883613598969 2209 3 -0.12119280770217244 2209 4 0.2101797621338335 2209 5 0.21470062825162728 2209 6 0.22149478535391864 2209 7 0.23289948177511377 2209 8 0.3667156948127328 2209 9 -1.9443643836933546 2209 10 -0.29649029948528494 2209 11 0.01617015750539963 2209 12 0.4120979500725426 2209 13 0.5481644855016441 2209 14 0.676156355079442 2209 15 0.9122381591641826 2209 16 0.8019196975981941 2209 17 0.594711174467875 2209 18 0.7014701728446866 2209 19 0.5417032627127156 2209 20 0.47322867151193293 2209 21 0.45546289976120846 2209 22 0.44227839704823424 2209 23 0.5112478418126614 2209 24 0.517179038613385 2209 25 0.5821871466440527 2210 1 0.16165415900211144 2210 2 0.1818801207897387 2210 3 0.1058155913865022 2210 4 0.3133716125267725 2210 5 0.19081821306803448 2210 6 0.09712937171331504 2210 7 0.02488620177625478 2210 8 0.24469749389071183 2210 9 0.38584471714488444 2210 10 -1.337906928799768 2210 11 -0.3779868019714172 2210 12 -2.300264781485751 2210 13 -0.7718199377273933 2210 14 -0.4361094022551555 2210 15 -0.767280313563198 2210 16 -0.0724690569485702 2210 17 0.08675608169771465 2210 18 0.33239438785263076 2210 19 0.2866940752200975 2210 20 0.3127202722211706 2210 21 0.2060296762412861 2210 22 0.20841598970287123 2210 23 0.17546473049749522 2210 24 0.2738992593557086 2210 25 0.2310847485730818 2211 1 -0.3278264274923899 2211 2 -0.057217176566105366 2211 3 -1.572511003311881 2211 4 -0.5872227272180837 2211 5 -0.4187071511973457 2211 6 -0.45453136382662507 2211 7 -0.47704137606221764 2211 8 -0.18650172167002665 2211 9 0.019706557772352085 2211 10 0.1586516490437333 2211 11 -0.2311739758429212 2211 12 -0.28444037162572045 2211 13 -0.3491563484646818 2211 14 -0.6815320234396225 2211 15 -0.3148033316183714 2211 16 -0.129567199427143 2211 17 -0.1735705598654107 2211 18 -0.07815796547578202 2211 19 -0.11956476014584183 2211 20 -0.10429230742145279 2211 21 -0.1541651139997242 2211 22 -0.22316938563872668 2211 23 -0.4912837394431335 2211 24 -0.2788411491419893 2211 25 -0.20872064524681377 2212 1 -0.2846838985776934 2212 2 -0.4019538769767692 2212 3 -2.323100701777342 2212 4 -1.3723476432640354 2212 5 -0.6658507986568907 2212 6 -0.46631994108185637 2212 7 -0.4321423855481754 2212 8 -0.43222687596204423 2212 9 -0.9547213043188165 2212 10 -0.33027796619261807 2212 11 -0.3238454621174256 2212 12 -0.025086984600093842 2212 13 -0.4177783087229443 2212 14 -0.18148180227732524 2212 15 0.04414889118314136 2212 16 -1.1751627912391598 2212 17 -1.454346991135985 2212 18 -0.4245178273319752 2212 19 -0.21008225870151875 2212 20 -0.311402266797991 2212 21 -0.19174894071748838 2212 22 -0.4079306444045514 2212 23 -0.13323506864955448 2212 24 -0.26332848543589193 2212 25 -0.02888481337810589 2213 1 -3.100390249332378 2213 2 -1.3632177341914904 2213 3 -2.4249797741035763 2213 4 -0.9731163943089385 2213 5 -0.8060560497542397 2213 6 -0.5630655683026333 2213 7 -0.24708646335712103 2213 8 -0.13327739940491057 2213 9 -0.23897964792367787 2213 10 -0.14087663474391832 2213 11 -0.3557665449167544 2213 12 -0.08530908872157994 2213 13 -0.540631024404405 2213 14 -0.10694289967230475 2213 15 -0.17465419029913676 2213 16 -1.6063184617062234 2213 17 -0.8711187400458513 2213 18 -0.24234017443201594 2213 19 -0.16387995713254355 2213 20 -0.14575617482343262 2213 21 0.1208376820575694 2213 22 0.02519182680092058 2213 23 0.022579361714988977 2213 24 0.06046649468505905 2213 25 0.045635801054650926 2214 1 -0.9444792705286886 2214 2 -0.8212464270378135 2214 3 -6.469448363428725 2214 4 -2.184730129510431 2214 5 -1.2788763709885558 2214 6 -0.949508938327071 2214 7 -0.7720711128005746 2214 8 -0.5804227804763187 2214 9 -0.41740447043807194 2214 10 -0.4255642872001104 2214 11 -1.0321866353880012 2214 12 -0.7409391589680965 2214 13 -5.469995930432081 2214 14 -3.896665867755182 2214 15 -2.5114841809056117 2214 16 -3.1267815879802114 2214 17 -3.585498498370008 2214 18 -1.3812344843632332 2214 19 -0.9709214390844139 2214 20 -0.6612087625231524 2214 21 -0.6767751967891726 2214 22 -0.5977388559791876 2214 23 -0.5735944156258412 2214 24 -0.4088108779930053 2214 25 -0.27349209020474774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1632.0 3 244.0 4 202.0 5 461.0 6 71.0 7 84.0 8 253.0 9 698.0 10 1698.0 11 3578.0 12 6476.0 13 10490.0 14 15656.0 15 20160.0 16 24446.0 17 27127.0 18 28933.0 19 29526.0 20 30566.0 21 31825.0 22 32878.0 23 34886.0 24 37640.0 25 40670.0 26 45530.0 27 51715.0 28 59311.0 29 71335.0 30 88204.0 31 109032.0 32 142151.0 33 185381.0 34 247211.0 35 332954.0 36 467377.0 37 702214.0 38 1402719.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.114000602658706 21.46158751515406 13.258421454649932 23.1659904275373 2 14.619936563675056 21.4828270140216 41.48801025441072 22.409226167892626 3 18.01996542066658 27.38907353770397 29.947944210763488 24.643016830865967 4 11.865426546408903 17.64976712140389 39.56704122893219 30.917765103255025 5 12.542651777290658 38.44065919365115 35.99043059296564 13.026258436092549 6 29.924474964044872 39.623918339649464 17.938381116662747 12.513225579642917 7 25.17271074493636 33.28062384620162 24.26681118966682 17.27985421919519 8 23.370742598508702 38.065452826919724 21.805218088139355 16.758586486432215 9 24.025016187947852 17.146311728153627 22.67765119823505 36.15102088566346 10 14.138934234924871 30.654026700752667 34.47462125519644 20.732417809126023 11 31.718249703287892 26.472627184596142 23.60857024575911 18.20055286635685 12 20.097723307517867 28.009080244630496 32.87028475467835 19.022911693173285 13 26.07148077459221 24.490849035589083 27.373948039539375 22.063722150279332 14 19.581091023889456 23.68541921136244 29.185254475025808 27.548235289722296 15 22.452344975373013 31.110541350058156 24.65484505885235 21.782268615716486 16 20.88075279613328 31.937796250029187 26.66392696196325 20.517523991874285 17 19.47052242805181 30.844651132815155 29.42844246701915 20.25638397211388 18 19.776678846205975 30.492944186303017 30.076918978489047 19.65345798900196 19 20.247208154898804 29.508827211391413 30.234894917152438 20.009069716557345 20 21.545224502586162 29.090615726284096 29.317160295810886 20.046999475318856 21 21.645587797488748 29.648506499950383 28.63454926477885 20.071356437782022 22 20.315429451025913 29.462000864452577 29.815557764556466 20.407011919965047 23 20.322127791928313 29.74185316883221 29.46003338218303 20.475985657056448 24 20.40673252722919 29.024396338505827 29.566936792589203 21.001934341675778 25 21.02023183478269 28.970170653564857 28.66737032851045 21.342227183142008 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3994.0 1 3994.0 2 3839.5 3 3685.0 4 3685.0 5 3685.0 6 5815.0 7 7945.0 8 7945.0 9 7945.0 10 13415.5 11 18886.0 12 18886.0 13 18886.0 14 32487.5 15 46089.0 16 46089.0 17 46089.0 18 72768.5 19 99448.0 20 99448.0 21 99448.0 22 140773.0 23 182098.0 24 182098.0 25 182098.0 26 234581.5 27 287065.0 28 287065.0 29 287065.0 30 340633.0 31 394201.0 32 394201.0 33 394201.0 34 436722.0 35 479243.0 36 479243.0 37 479243.0 38 502982.0 39 526721.0 40 526721.0 41 526721.0 42 517808.5 43 508896.0 44 508896.0 45 508896.0 46 507834.5 47 506773.0 48 506773.0 49 506773.0 50 463797.0 51 420821.0 52 420821.0 53 420821.0 54 368756.0 55 316691.0 56 316691.0 57 316691.0 58 268761.5 59 220832.0 60 220832.0 61 220832.0 62 180447.0 63 140062.0 64 140062.0 65 140062.0 66 106084.5 67 72107.0 68 72107.0 69 72107.0 70 51897.5 71 31688.0 72 31688.0 73 31688.0 74 21899.0 75 12110.0 76 12110.0 77 12110.0 78 8128.0 79 4146.0 80 4146.0 81 4146.0 82 2736.0 83 1326.0 84 1326.0 85 1326.0 86 842.5 87 359.0 88 359.0 89 359.0 90 227.0 91 95.0 92 95.0 93 95.0 94 60.0 95 25.0 96 25.0 97 25.0 98 26.5 99 28.0 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10043557865034557 2 0.05364809370751498 3 0.05668169622251147 4 0.05136122411928685 5 0.04902768372313571 6 0.06797603173988305 7 0.06393900685454157 8 0.061045416763314125 9 0.06596918699919306 10 0.06610919942296213 11 0.05980864035335402 12 0.0613721124187753 13 0.061045416763314125 14 0.06377565902681097 15 0.06620254103880818 16 0.061465454034621336 17 0.06470907518527144 18 0.1406191442720684 19 0.06886277709042049 20 0.06377565902681097 21 0.060882068935583546 22 0.06662257831011538 23 0.06557248513184737 24 0.07600341070264302 25 0.06939949138153526 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4285334.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.71457079739181 #Duplication Level Percentage of deduplicated Percentage of total 1 89.87938489975133 72.54575975717007 2 7.799339134549949 12.590406214970006 3 1.288492988873784 3.1200047571718805 4 0.38471808877633 1.2420942165429745 5 0.18205719074783128 0.7347334005895046 6 0.09915400006722092 0.48019035349621775 7 0.06789671537656239 0.3836177968120335 8 0.04804829581856845 0.3102558059633494 9 0.03407050079846207 0.2474987263920055 >10 0.18858983811144447 2.774500913186353 >50 0.015572688409511908 0.8778977109172994 >100 0.010523730146990873 1.5908315040991932 >500 0.0010759645511087075 0.5934398966956135 >1k 9.596435862246353E-4 1.6466935572689565 >5k 5.8160217346905805E-5 0.29694281591028987 >10k+ 5.8160217346905805E-5 0.5651325728141687 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 12505 0.29180922653870156 No Hit CCCATGTACTCTGCGTTGATACCAC 11572 0.2700372946426113 No Hit GTATCAACGCAGAGTACTTTTTTTT 6661 0.15543712578762822 No Hit GAGTACATGGGAAGCAGTGGTATCA 5990 0.139779069729454 No Hit GGTATCAACGCAGAGTACTTTTTTT 4614 0.10766955387841413 No Hit ATTCCATTCCATTCCATTCCATTCC 4533 0.10577938615753171 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.333540396151152E-5 0.0 5 2.333540396151152E-5 0.0 0.0 2.333540396151152E-5 0.0 6 2.333540396151152E-5 0.0 0.0 2.333540396151152E-5 0.0 7 2.333540396151152E-5 0.0 0.0 2.333540396151152E-5 0.0 8 2.333540396151152E-5 0.0 0.0 2.333540396151152E-5 0.0 9 4.667080792302304E-5 0.0 0.0 4.667080792302304E-5 0.0 10 4.667080792302304E-5 0.0 0.0 4.667080792302304E-5 2.333540396151152E-5 11 4.667080792302304E-5 0.0 0.0 4.667080792302304E-5 2.333540396151152E-5 12 4.667080792302304E-5 0.0 0.0 4.667080792302304E-5 2.333540396151152E-5 13 4.667080792302304E-5 0.0 0.0 9.334161584604607E-5 2.333540396151152E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 65 8.037459E-4 11.690748 19 GCACCGC 80 3.7843606E-4 10.684203 6 CGCCCTA 255 0.0 10.436075 16 GACTCCG 135 6.47844E-7 9.848813 7 CGAACCG 100 2.746535E-4 9.503727 12 TATACCG 115 1.11481575E-4 9.084472 5 TGCACCG 115 1.11481575E-4 9.084472 5 TATCGCG 115 1.1152412E-4 9.084153 7 TAGACAG 795 0.0 9.079277 5 GTCGCCC 220 1.6916601E-10 9.065173 11 GTATTAG 715 0.0 9.035131 1 TCGCCCA 265 1.8189894E-12 8.96578 12 CGTAGAC 85 0.007455197 8.938836 3 GTCCCGC 310 0.0 8.890584 12 CCTACAC 455 0.0 8.766934 3 CTGTCGC 260 7.2759576E-12 8.766423 9 GTCCTAG 470 0.0 8.691649 1 GTACTAG 340 0.0 8.661924 1 CCGCTAC 100 0.002906073 8.550158 1 CTAGGAC 345 0.0 8.533997 3 >>END_MODULE