FastQCFastQC Report
Wed 25 May 2016
SRR1294649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285334
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT155560.3630055440252732No Hit
CCCATGTACTCTGCGTTGATACCAC133400.3112942888465637No Hit
GGTATCAACGCAGAGTACTTTTTTT120310.2807482450609451No Hit
GTACATGGGAAGCAGTGGTATCAAC118380.27624451209637335No Hit
TATCAACGCAGAGTACTTTTTTTTT98930.23085715139123342No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60180.14043246104037632No Hit
GAGTACATGGGAAGCAGTGGTATCA59320.13842561629968633No Hit
CATGTACTCTGCGTTGATACCACTG50440.11770377758186411No Hit
ATTCCATTCCATTCCATTCCATTCC45120.10528934267433998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG951.3906229E-510.97910612
CGCCCTA2053.6379788E-1210.17575616
ACCGTCC750.00260198310.1553218
TAGACGG951.6097551E-410.0216985
TAGGCCG900.00109247839.5206145
GCGATTT1401.0878484E-69.48184513
TATACTG11250.09.3090445
TATTCCG2054.0745363E-109.28840355
TCTATAC6600.09.2132213
CTAGGGC2700.09.149243
CGATTTT1151.1348975E-49.06959114
TCGGTTT1054.582316E-49.03032913
ACTCCCG950.00178917929.0195288
CGTGCAA950.00178975159.01921210
CTATACT7850.08.9748464
ACTGCGT850.00730810138.9605788
CTTAGAC3500.08.9582123
CCCCGTA2607.2759576E-128.75247215
AGCGTAC2659.094947E-128.6136631
GGACCGT1000.00285007878.5685526
Produced by FastQC (version 0.11.5)