##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294649_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4285334 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.270597344337688 31.0 31.0 34.0 28.0 34.0 2 31.44276618811976 31.0 31.0 34.0 28.0 34.0 3 31.590765387248695 31.0 31.0 34.0 28.0 34.0 4 35.1771252835835 37.0 35.0 37.0 32.0 37.0 5 35.263481912961744 37.0 35.0 37.0 32.0 37.0 6 34.88585347139803 37.0 35.0 37.0 32.0 37.0 7 34.7205078997343 37.0 35.0 37.0 32.0 37.0 8 34.77947296523445 37.0 35.0 37.0 32.0 37.0 9 36.23268011314871 39.0 35.0 39.0 31.0 39.0 10 36.00178865871365 39.0 35.0 39.0 30.0 39.0 11 36.15437839850989 39.0 35.0 39.0 31.0 39.0 12 36.0820185777818 39.0 35.0 39.0 30.0 39.0 13 35.94940441048469 39.0 35.0 39.0 30.0 39.0 14 37.05036246882973 40.0 36.0 41.0 30.0 41.0 15 37.11190399628127 40.0 36.0 41.0 31.0 41.0 16 37.14198099844726 40.0 36.0 41.0 31.0 41.0 17 37.17163352961519 40.0 36.0 41.0 31.0 41.0 18 37.173614705411524 40.0 36.0 41.0 31.0 41.0 19 37.18374460427122 40.0 37.0 41.0 30.0 41.0 20 37.25261158173435 40.0 37.0 41.0 31.0 41.0 21 37.09155599073491 40.0 37.0 41.0 30.0 41.0 22 37.08587078626777 40.0 36.0 41.0 30.0 41.0 23 37.094930756855824 40.0 36.0 41.0 30.0 41.0 24 37.03948793722963 40.0 36.0 41.0 30.0 41.0 25 35.92830523828481 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.555527380853615 1101 2 0.5214441112929684 1101 3 0.4462791481723869 1101 4 0.4012687792386771 1101 5 0.3951469195944952 1101 6 0.4962536927698835 1101 7 0.5940645167667213 1101 8 0.5426605738581713 1101 9 0.7156570137530238 1101 10 0.7689279371045288 1101 11 0.7798446744094676 1101 12 0.8768458262079477 1101 13 0.8316557853639353 1101 14 1.0251382914884601 1101 15 0.9553812201473519 1101 16 1.0183274672202387 1101 17 1.0716969042181717 1101 18 1.0438604975879144 1101 19 1.1426494306328934 1101 20 1.0745671100353533 1101 21 1.3581468871176199 1101 22 1.2772263197021516 1101 23 1.2620572368010627 1101 24 1.3395967512631728 1101 25 1.2693485042985984 1102 1 0.40869207757433657 1102 2 0.4599935435656306 1102 3 0.346203740920366 1102 4 0.3074398115780568 1102 5 0.41871987845996017 1102 6 0.453831710498811 1102 7 0.5225876034838848 1102 8 0.49779202561220615 1102 9 0.7038419189125449 1102 10 0.8151658552066152 1102 11 0.7302002160790195 1102 12 0.7850558788638224 1102 13 0.8037192484361171 1102 14 1.006746674143848 1102 15 0.9504830962844792 1102 16 0.9807463192681567 1102 17 0.8545796341956091 1102 18 0.8726368211202598 1102 19 0.9956131294408195 1102 20 0.9029765866831809 1102 21 1.1427200256919505 1102 22 1.1097271683273746 1102 23 1.0401673454838232 1102 24 1.078089011448732 1102 25 1.0060143409373552 1103 1 0.32295374844422753 1103 2 0.2976485246321694 1103 3 0.2333591481429096 1103 4 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0.14865775996304365 2207 1 0.26929262188842884 2207 2 0.26034871415286887 2207 3 0.3014524033465271 2207 4 0.23118884331950085 2207 5 0.2528115483136517 2207 6 0.34604784468891125 2207 7 0.3444701517247495 2207 8 0.35711979804382565 2207 9 0.41500524028992203 2207 10 0.5692384626708886 2207 11 0.4673542336830323 2207 12 0.44199088373011364 2207 13 0.5616830318514374 2207 14 0.6007825254912902 2207 15 0.47944635882614506 2207 16 0.5476006406704386 2207 17 0.498492350652306 2207 18 0.576158148282218 2207 19 0.7316777060432713 2207 20 0.5661054435867214 2207 21 0.8960358608210299 2207 22 0.8364069691216898 2207 23 0.7382811448705979 2207 24 0.8668590585765159 2207 25 0.845480090142388 2208 1 0.16068814447712754 2208 2 0.15915659528603499 2208 3 0.20587492213060798 2208 4 0.19695715590568597 2208 5 0.11778644520481407 2208 6 0.3034104768580477 2208 7 0.34704454205672164 2208 8 0.25235044246365135 2208 9 0.3748945196836715 2208 10 0.44026769194160664 2208 11 -0.0036446618356436034 2208 12 -0.13698941753612814 2208 13 0.11059204759472863 2208 14 -0.40753068279609295 2208 15 0.23361517001433185 2208 16 0.304038093402049 2208 17 0.3012355538603515 2208 18 0.3615877235216729 2208 19 0.5324014722607089 2208 20 0.4289876009385978 2208 21 0.6786079628596227 2208 22 0.7215530877457113 2208 23 0.6056491047364858 2208 24 0.6924140631573223 2208 25 0.7363627360171705 2209 1 0.012322141788736474 2209 2 0.054105881621520524 2209 3 0.10875735794629549 2209 4 -0.517352858473437 2209 5 -0.06691550434926796 2209 6 0.10661433069697779 2209 7 0.10995572327819048 2209 8 0.22922345325886084 2209 9 0.20264137043454866 2209 10 0.2515319899245725 2209 11 0.26403579670436983 2209 12 0.2293876928854317 2209 13 0.35695580511496416 2209 14 0.34990534351141633 2209 15 -0.1691188690281038 2209 16 -0.2603618536741763 2209 17 0.13714278288940562 2209 18 -0.15073093373585777 2209 19 0.270179118451189 2209 20 0.18098205116405808 2209 21 0.5325022528454539 2209 22 0.5754798332572406 2209 23 0.5783290942961585 2209 24 0.5847078937430084 2209 25 0.607358801316245 2210 1 -0.13387358689953288 2210 2 -0.14184057427238272 2210 3 -0.05265435125336282 2210 4 -0.017609359202090502 2210 5 -0.04333627170665011 2210 6 -0.4550428992379594 2210 7 -0.1444202351977637 2210 8 -0.2505103890072675 2210 9 -0.028337731067878735 2210 10 0.17625028573259982 2210 11 0.02591758434429181 2210 12 0.07868038596255644 2210 13 0.1618794497021412 2210 14 0.17677063893184197 2210 15 0.11835739094635045 2210 16 0.19309558457077003 2210 17 -0.8435809967180106 2210 18 -1.4018545238676623 2210 19 -0.3012683451097189 2210 20 -0.19102338467364888 2210 21 -0.3766324953846478 2210 22 -0.2439976376151236 2210 23 -0.2566441919862186 2210 24 0.22145453652583313 2210 25 0.3521828391758035 2211 1 -0.16972706564003914 2211 2 -0.17054213997232281 2211 3 -0.15469839716976352 2211 4 -0.09886109229480411 2211 5 -0.043866225530720726 2211 6 -0.07760246577169738 2211 7 -0.22578918677056237 2211 8 -0.17196017446658374 2211 9 -0.11495222024329621 2211 10 0.007126927476242884 2211 11 -0.36740086318125265 2211 12 -0.13658742371762145 2211 13 -0.16878228677516915 2211 14 -0.1860576947527406 2211 15 -0.2509781470590795 2211 16 -0.21382664877612712 2211 17 -0.3458554585717053 2211 18 -0.5081096426191536 2211 19 -0.15633183585736532 2211 20 -0.3355286250646188 2211 21 0.005213577452927609 2211 22 -0.049632407399883505 2211 23 -0.7525664429604859 2211 24 -0.19900128464455946 2211 25 -0.7609552088398814 2212 1 -0.20778637348565354 2212 2 -0.3092612927703442 2212 3 -0.15083250888898547 2212 4 -0.06580320560775021 2212 5 -0.07629890968066633 2212 6 -0.16311456040163108 2212 7 -0.2982034307084689 2212 8 -0.4269836195617245 2212 9 -0.231100862935385 2212 10 -0.5679885593684091 2212 11 -0.26061653331744594 2212 12 -0.37192751259000545 2212 13 -0.37264307405028774 2212 14 -0.35554980538880443 2212 15 -0.22651884025511038 2212 16 -0.41150850977617637 2212 17 -0.6984583692545527 2212 18 -0.4572770949249403 2212 19 -0.5202165626268993 2212 20 -0.707108686076019 2212 21 -0.1579052785063908 2212 22 -0.5509040360276813 2212 23 -0.422542053872931 2212 24 -0.5486892386656521 2212 25 -0.2666099952950134 2213 1 -0.1765269039373294 2213 2 -0.2188635050910399 2213 3 -0.1208912328579288 2213 4 -0.01910084461467676 2213 5 -0.07877369305676751 2213 6 -0.20849354539810605 2213 7 -0.32357022192904594 2213 8 -0.335287072662652 2213 9 -0.3486401918491637 2213 10 -0.32002536095146183 2213 11 -0.0957368503057836 2213 12 0.01518665120075724 2213 13 0.08154378373026816 2213 14 -0.6803227293261997 2213 15 -0.2298920861540381 2213 16 -0.34033881671598465 2213 17 -0.21453070769636895 2213 18 -0.1330105590162205 2213 19 -0.2548349515086912 2213 20 -0.2264253562433538 2213 21 0.029944598023526225 2213 22 -0.34251345249296605 2213 23 -0.4010622218151596 2213 24 -0.5814160973381988 2213 25 -0.32852178700518664 2214 1 -0.2982600291842097 2214 2 -0.23570948760245258 2214 3 -0.1680233383234757 2214 4 -0.15178253807080466 2214 5 -0.2592643915337689 2214 6 -0.18498712413097707 2214 7 -0.17233163781149585 2214 8 -0.21772271197484372 2214 9 -0.2673416187150863 2214 10 -0.1901401436206811 2214 11 -0.11043667784526434 2214 12 -0.29110788249205655 2214 13 -0.3189886883812676 2214 14 -0.24721815378293854 2214 15 -0.1815207202151896 2214 16 -0.35292144682627224 2214 17 -0.3880056937155132 2214 18 -0.41015242643648975 2214 19 -0.5590891000335532 2214 20 -0.6531248768056912 2214 21 -0.680097646680899 2214 22 -0.8297094603218369 2214 23 -1.100748848181901 2214 24 -0.6328048596228086 2214 25 -0.9775637443773775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 17.0 9 74.0 10 153.0 11 321.0 12 798.0 13 1885.0 14 3896.0 15 6393.0 16 9348.0 17 12194.0 18 14383.0 19 16436.0 20 18173.0 21 19493.0 22 21915.0 23 24224.0 24 27289.0 25 30618.0 26 35409.0 27 39698.0 28 46718.0 29 58580.0 30 73807.0 31 96174.0 32 127987.0 33 171969.0 34 235620.0 35 327014.0 36 467896.0 37 739525.0 38 1657324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.74718407995527 21.881372346125872 12.310350804004678 25.06109276991419 2 14.38361630622024 21.88361513945004 39.85467643828929 23.87809211604043 3 17.548970286845744 26.936362402203713 30.371730455988953 25.14293685496159 4 11.733285972604838 17.737658593871796 39.43485457113491 31.094200862388455 5 12.764231722161213 38.047874184095384 35.856155710719584 13.331738383023817 6 30.149123245124954 39.77884035065527 17.43716944028372 12.634866963936053 7 25.19398155366364 33.48374625504268 24.020546033216103 17.301726158077578 8 23.341883473799925 38.472107792453315 21.288916170501782 16.897092563244975 9 23.822148295472708 16.682779804462815 22.693575256754276 36.8014966433102 10 13.93503357767445 30.606975001399704 34.48494047098934 20.973050949936503 11 32.0160213555806 26.06972881009234 23.401612223043646 18.512637611283413 12 20.124654748025563 27.913277931223103 32.7904433693419 19.17162395140943 13 26.31591351986579 24.06913258275732 27.40686775420615 22.20808614317074 14 19.59274539820035 23.341181906433185 29.374673300227755 27.691399395138706 15 22.478697399816813 30.835010588585334 24.69853159948895 21.98776041210891 16 20.812726207783296 31.764648230124635 26.732130314351522 20.690495247740547 17 19.27411475374259 30.82105871352355 29.544751831421266 20.360074701312595 18 19.585655213612984 30.31740920164655 30.330243715755184 19.76669186898528 19 20.125041187237883 29.21972002945879 30.723905959618303 19.93133282368502 20 21.369022616747408 28.77173699980398 29.845029076008327 20.014211307440284 21 21.45193188381278 29.43032325436227 29.004007864047647 20.1137369977773 22 20.09681681580769 29.189425199161956 30.26171903987813 20.45203894515223 23 20.09066281725394 29.55355353112912 29.96502718927765 20.390756462339297 24 20.216729998982576 28.826121290019067 30.057554467191807 20.899594243806547 25 20.808823924061535 28.749160785847483 29.14943997550673 21.29257531458425 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1941.0 1 1941.0 2 2162.5 3 2384.0 4 2384.0 5 2384.0 6 4993.0 7 7602.0 8 7602.0 9 7602.0 10 12573.5 11 17545.0 12 17545.0 13 17545.0 14 29468.0 15 41391.0 16 41391.0 17 41391.0 18 67495.0 19 93599.0 20 93599.0 21 93599.0 22 135661.0 23 177723.0 24 177723.0 25 177723.0 26 231953.5 27 286184.0 28 286184.0 29 286184.0 30 340326.5 31 394469.0 32 394469.0 33 394469.0 34 439060.5 35 483652.0 36 483652.0 37 483652.0 38 504724.5 39 525797.0 40 525797.0 41 525797.0 42 517867.5 43 509938.0 44 509938.0 45 509938.0 46 510663.0 47 511388.0 48 511388.0 49 511388.0 50 469395.0 51 427402.0 52 427402.0 53 427402.0 54 374143.0 55 320884.0 56 320884.0 57 320884.0 58 272010.0 59 223136.0 60 223136.0 61 223136.0 62 182338.5 63 141541.0 64 141541.0 65 141541.0 66 106559.5 67 71578.0 68 71578.0 69 71578.0 70 51187.5 71 30797.0 72 30797.0 73 30797.0 74 21146.5 75 11496.0 76 11496.0 77 11496.0 78 7529.0 79 3562.0 80 3562.0 81 3562.0 82 2289.0 83 1016.0 84 1016.0 85 1016.0 86 626.5 87 237.0 88 237.0 89 237.0 90 150.0 91 63.0 92 63.0 93 63.0 94 33.5 95 4.0 96 4.0 97 4.0 98 4.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09660857240065769 2 0.0 3 0.13177502617065553 4 0.011434347941140644 5 0.022845360478319778 6 0.012974484602600405 7 0.021141875989129436 8 0.01876166478505526 9 2.566894435766267E-4 10 0.3867843206620534 11 0.06533913109223226 12 0.001353453429767668 13 7.000621188453456E-5 14 0.0012601118139216221 15 0.0013767888337291795 16 9.334161584604607E-5 17 2.333540396151152E-5 18 3.2669565546116126E-4 19 6.067205029992995E-4 20 3.2669565546116126E-4 21 2.1001863565360367E-4 22 0.0029869317070734744 23 1.1667701980755759E-4 24 4.667080792302304E-5 25 0.0017501552971133638 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4285334.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.81876372252808 #Duplication Level Percentage of deduplicated Percentage of total 1 88.89201838070231 70.06358993567197 2 8.630488428849494 13.604888565670018 3 1.393434659262583 3.294863915135968 4 0.418047017803906 1.3179979648477425 5 0.17950433831269202 0.7074155014318408 6 0.10390810741964687 0.4913945140538528 7 0.07090459538416159 0.39120287852979807 8 0.05025018855583719 0.31685261910360124 9 0.03565393230580144 0.2529178979570977 >10 0.19507239690707456 2.8148849966807545 >50 0.01635615698417125 0.896571284504507 >100 0.01207148245891433 1.7804968694994219 >500 0.0010410525016756945 0.5720368368472706 >1k 0.0010113077800587072 1.627514682626827 >5k 1.1897738588922027E-4 0.6303422202471884 >10k+ 1.1897738588922027E-4 1.2370293171920594 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 15556 0.3630055440252732 No Hit CCCATGTACTCTGCGTTGATACCAC 13340 0.3112942888465637 No Hit GGTATCAACGCAGAGTACTTTTTTT 12031 0.2807482450609451 No Hit GTACATGGGAAGCAGTGGTATCAAC 11838 0.27624451209637335 No Hit TATCAACGCAGAGTACTTTTTTTTT 9893 0.23085715139123342 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6018 0.14043246104037632 No Hit GAGTACATGGGAAGCAGTGGTATCA 5932 0.13842561629968633 No Hit CATGTACTCTGCGTTGATACCACTG 5044 0.11770377758186411 No Hit ATTCCATTCCATTCCATTCCATTCC 4512 0.10528934267433998 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.333540396151152E-5 0.0 0.0 0.0 5 0.0 4.667080792302304E-5 0.0 0.0 0.0 6 0.0 4.667080792302304E-5 0.0 2.333540396151152E-5 0.0 7 0.0 4.667080792302304E-5 0.0 4.667080792302304E-5 0.0 8 0.0 4.667080792302304E-5 0.0 7.000621188453456E-5 0.0 9 4.667080792302304E-5 4.667080792302304E-5 0.0 9.334161584604607E-5 0.0 10 4.667080792302304E-5 4.667080792302304E-5 0.0 1.1667701980755759E-4 0.0 11 4.667080792302304E-5 4.667080792302304E-5 0.0 1.1667701980755759E-4 0.0 12 4.667080792302304E-5 4.667080792302304E-5 0.0 1.1667701980755759E-4 0.0 13 4.667080792302304E-5 4.667080792302304E-5 0.0 1.1667701980755759E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACCG 95 1.3906229E-5 10.979106 12 CGCCCTA 205 3.6379788E-12 10.175756 16 ACCGTCC 75 0.002601983 10.155321 8 TAGACGG 95 1.6097551E-4 10.021698 5 TAGGCCG 90 0.0010924783 9.520614 5 GCGATTT 140 1.0878484E-6 9.481845 13 TATACTG 1125 0.0 9.309044 5 TATTCCG 205 4.0745363E-10 9.2884035 5 TCTATAC 660 0.0 9.213221 3 CTAGGGC 270 0.0 9.14924 3 CGATTTT 115 1.1348975E-4 9.069591 14 TCGGTTT 105 4.582316E-4 9.030329 13 ACTCCCG 95 0.0017891792 9.019528 8 CGTGCAA 95 0.0017897515 9.019212 10 CTATACT 785 0.0 8.974846 4 ACTGCGT 85 0.0073081013 8.960578 8 CTTAGAC 350 0.0 8.958212 3 CCCCGTA 260 7.2759576E-12 8.752472 15 AGCGTAC 265 9.094947E-12 8.613663 1 GGACCGT 100 0.0028500787 8.568552 6 >>END_MODULE