FastQCFastQC Report
Wed 25 May 2016
SRR1294648_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294648_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4720292
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT154110.326484039546706No Hit
GTACATGGGAAGCAGTGGTATCAAC132650.2810207504112034No Hit
CCCATGTACTCTGCGTTGATACCAC120170.25458170808077124No Hit
GGTATCAACGCAGAGTACTTTTTTT106670.22598178248294812No Hit
TATCAACGCAGAGTACTTTTTTTTT94630.2004748858757043No Hit
GAGTACATGGGAAGCAGTGGTATCA61850.1310300294981751No Hit
ATTCCATTCCATTCCATTCCATTCC57130.12103064810397324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG751.4842342E-512.6632255
TACACCG600.00589056511.0803225
CGTCGGT700.001495367710.85453819
TGTCCCG1909.094947E-1210.49614511
CCCGTAC1856.002665E-1110.27477716
CCCCGTA1856.002665E-1110.27466915
GTCTTGC9100.09.6050441
AGCGTCG800.00450200269.50416917
TCGCGCG1106.864582E-59.4972179
CCGTACC1451.7152088E-69.1697279
TAGGACA4600.09.0844874
GTCCCGC2002.6411726E-99.02867312
TTCCGTA1901.0648364E-88.9974597
ACCGCTC850.00745733278.9385588
CCGTACA2252.6011548E-108.87055717
CGGTGAA1951.6600097E-88.7666639
TATCGCG1201.7706057E-48.7058757
GCGCGCC1201.7722347E-48.70513711
CGCCCTA2304.0199666E-108.6777216
CCCGCCC2304.0199666E-108.67762814
Produced by FastQC (version 0.11.5)