##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294648_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4720292 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.956514554608063 31.0 30.0 33.0 25.0 34.0 2 30.11592481990521 31.0 30.0 34.0 25.0 34.0 3 30.031316918529615 31.0 30.0 34.0 25.0 34.0 4 33.660309150366125 35.0 35.0 37.0 28.0 37.0 5 34.01275577866793 35.0 35.0 37.0 28.0 37.0 6 34.135783125281236 37.0 35.0 37.0 29.0 37.0 7 34.036052854357315 37.0 35.0 37.0 29.0 37.0 8 33.92631515168977 37.0 35.0 37.0 28.0 37.0 9 34.9853231537371 39.0 35.0 39.0 27.0 39.0 10 35.146341158555444 39.0 35.0 39.0 27.0 39.0 11 35.34660313387392 39.0 35.0 39.0 28.0 39.0 12 35.139634793779706 39.0 35.0 39.0 27.0 39.0 13 34.97957901756925 38.0 35.0 39.0 27.0 39.0 14 35.87861492466991 39.0 35.0 41.0 27.0 41.0 15 35.87261063510478 39.0 35.0 41.0 27.0 41.0 16 35.98951653838364 39.0 35.0 41.0 27.0 41.0 17 35.982473965593655 39.0 35.0 41.0 27.0 41.0 18 35.89655004393796 39.0 35.0 41.0 27.0 41.0 19 36.19841145420665 39.0 35.0 41.0 27.0 41.0 20 36.310813610683404 39.0 36.0 41.0 27.0 41.0 21 36.358380795086404 39.0 36.0 41.0 27.0 41.0 22 36.36388130225842 39.0 36.0 41.0 27.0 41.0 23 36.354788220728715 39.0 36.0 41.0 27.0 41.0 24 36.3731737358621 39.0 35.0 41.0 27.0 41.0 25 35.18953488470628 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.984573716166576 1101 2 0.9879481427885182 1101 3 1.246793975791885 1101 4 1.081640322368962 1101 5 0.8164857932641283 1101 6 0.7998897886101872 1101 7 0.7692084186774935 1101 8 0.9344007750152983 1101 9 1.14132904249265 1101 10 1.1009006567777533 1101 11 0.8751221827486262 1101 12 1.1239476336452512 1101 13 1.193506553624843 1101 14 1.200066997985509 1101 15 1.3700681722652206 1101 16 1.2943199662783016 1101 17 1.230459440484367 1101 18 1.2987304460412261 1101 19 1.181061337403996 1101 20 1.0067407348863 1101 21 0.936324734800273 1101 22 0.9378611001569226 1101 23 0.9825587874554031 1101 24 1.0138746565871557 1101 25 0.9241047868649588 1102 1 0.8980699180942722 1102 2 0.887490908196721 1102 3 1.0888633124114122 1102 4 0.9520148171445015 1102 5 0.8199135629508945 1102 6 0.7701562565241176 1102 7 0.7222796013973252 1102 8 0.7737547908694395 1102 9 1.1525622668953446 1102 10 1.0336826876311065 1102 11 0.7728682331189205 1102 12 0.9718239230750072 1102 13 1.004078392828589 1102 14 1.2906256767227404 1102 15 1.3887695219405316 1102 16 1.2205676233954605 1102 17 1.2313602436790276 1102 18 1.348940093832141 1102 19 1.0337333113607556 1102 20 0.3477643899583285 1102 21 0.6511155955112713 1102 22 0.6142754356601259 1102 23 0.7594830699313917 1102 24 0.9252068203660855 1102 25 0.8998510581157717 1103 1 0.694991176563029 1103 2 0.7759048666813548 1103 3 1.0376281146288449 1103 4 0.8684457193433701 1103 5 0.5541444554756936 1103 6 0.5501729108042923 1103 7 0.5091276950139729 1103 8 0.6376334496292415 1103 9 0.6833042918934638 1103 10 0.7870993370249906 1103 11 0.656000697276582 1103 12 0.8293172507234274 1103 13 0.7263357120229372 1103 14 0.5837068973581836 1103 15 0.8856773652686343 1103 16 0.8654174999180455 1103 17 0.8481628464970825 1103 18 0.9730920116431108 1103 19 0.8326748205593972 1103 20 -0.07059620399819266 1103 21 0.3777524889557995 1103 22 -0.1984237472846644 1103 23 0.39808351537218556 1103 24 0.437591701859688 1103 25 0.531276373587076 1104 1 0.42879398379787403 1104 2 0.43029456804280386 1104 3 0.749502598056889 1104 4 0.5595917138402129 1104 5 0.447951581793113 1104 6 0.30844638421492476 1104 7 0.3636097359728083 1104 8 0.5183915978756843 1104 9 0.7915134592489892 1104 10 0.7346088917901383 1104 11 0.5559651221003392 1104 12 0.652412588097377 1104 13 0.5803814649611567 1104 14 0.8955515347805374 1104 15 0.932993022538767 1104 16 0.7465747081988141 1104 17 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0.4370161973851978 2207 7 0.41981833759501797 2207 8 0.4547894698492172 2207 9 -1.131434568670329 2207 10 -0.04281374302165375 2207 11 0.37472037105906253 2207 12 0.5657892064726582 2207 13 0.7447856063731706 2207 14 1.0029504119821198 2207 15 0.9463429640181431 2207 16 0.9459940187883475 2207 17 0.8501652643357005 2207 18 0.9337592129824444 2207 19 -0.13942577633331865 2207 20 0.37398773248661854 2207 21 -0.1665119374752564 2207 22 0.3983039094554428 2207 23 -0.3708155987187354 2207 24 -1.4715108549804796 2207 25 -0.11221248950819529 2208 1 0.5284976157967414 2208 2 0.21253743956258475 2208 3 0.6513419079831166 2208 4 0.5688458636163034 2208 5 0.43787746205600797 2208 6 0.441770541281187 2208 7 0.4723393476710953 2208 8 0.5725367858366042 2208 9 -0.12142781633996691 2208 10 0.15953579369283943 2208 11 0.28678399645666985 2208 12 0.3606519282567433 2208 13 0.5808081498231843 2208 14 0.8996879394127717 2208 15 0.807269276254921 2208 16 0.6044510195151176 2208 17 0.538120692762547 2208 18 0.7779516559459339 2208 19 -1.2421041601620502 2208 20 0.04915949336963621 2208 21 -0.16140338928600073 2208 22 0.20988687642011428 2208 23 0.37147736704719136 2208 24 0.6021797502786796 2208 25 0.6599099295998627 2209 1 0.21183935648243946 2209 2 -1.3675606591194267 2209 3 -0.2058683243271524 2209 4 0.11263864086061659 2209 5 0.14177262638943233 2209 6 0.14785142366312698 2209 7 0.1734052041933083 2209 8 0.24215252308544422 2209 9 -1.9927247976256837 2209 10 -0.3200283796788881 2209 11 -0.006729727541710417 2209 12 0.2429933985339403 2209 13 0.3959258695563861 2209 14 0.5969728430364682 2209 15 0.6796810899947374 2209 16 0.5610632962363624 2209 17 0.4322102619628012 2209 18 0.6131185663600789 2209 19 0.3564085163868711 2209 20 0.33314290641577315 2209 21 0.41972345596714433 2209 22 0.2826048390388749 2209 23 0.35683636829755727 2209 24 0.4661528685181935 2209 25 0.5274857918940015 2210 1 0.1943576910460827 2210 2 0.15596255217961286 2210 3 0.07852011424715499 2210 4 0.3076939902665785 2210 5 0.20468980563136796 2210 6 0.1328378832579986 2210 7 0.11469842067027258 2210 8 0.18209342190001365 2210 9 0.4173021833028656 2210 10 -1.4211160304373038 2210 11 -0.42221939838808 2210 12 -2.172891568354949 2210 13 -0.6373866325521931 2210 14 -0.3982270884158936 2210 15 -0.7424922265366547 2210 16 -0.14362118431146342 2210 17 0.11038992041576279 2210 18 0.3486881290533077 2210 19 0.2665921349533633 2210 20 0.24082877346567244 2210 21 0.21438876293406395 2210 22 0.24521763204094782 2210 23 0.12614808801394872 2210 24 0.15124233494290706 2210 25 0.07027563983849205 2211 1 -0.4446684268286667 2211 2 -0.17191050843214484 2211 3 -1.5403462015109248 2211 4 -0.6594353471520265 2211 5 -0.6612810902415873 2211 6 -0.494283648592301 2211 7 -0.5729922555742917 2211 8 -0.14037825658170533 2211 9 -0.04508204983219599 2211 10 -0.03652743149167037 2211 11 -0.3962420813203167 2211 12 -0.3621773520156708 2211 13 -0.379967512384475 2211 14 -0.9003979693507489 2211 15 -0.37630437252622784 2211 16 -0.22306397671864175 2211 17 -0.2742576375193053 2211 18 -0.12902065348323077 2211 19 -0.18252311085637274 2211 20 -0.11784269856382679 2211 21 -0.22902948502364495 2211 22 -0.23729774449085994 2211 23 -0.5757817997615078 2211 24 -0.34891039756686126 2211 25 -0.16413558967393982 2212 1 -0.4329697450317518 2212 2 -0.45060951594641807 2212 3 -2.4593789819493495 2212 4 -1.4048463940162534 2212 5 -0.6916745053714024 2212 6 -0.4898497989606341 2212 7 -0.41311072912806424 2212 8 -0.2589510078803059 2212 9 -0.9726343319839259 2212 10 -0.25797160021836874 2212 11 -0.4297335416944108 2212 12 -0.08594743521987169 2212 13 -0.40943629466293174 2212 14 -0.06744797727280627 2212 15 0.0986470005618969 2212 16 -1.1791021463166658 2212 17 -1.275939038037194 2212 18 -0.2760661869636962 2212 19 -0.1443786435259824 2212 20 -0.16330461545073405 2212 21 -0.11403378374670581 2212 22 -0.3406370738507931 2212 23 -0.05611256485568106 2212 24 -0.1616854976822495 2212 25 0.031241668186417826 2213 1 -3.07431205792615 2213 2 -1.4715208417319197 2213 3 -2.4071691078292297 2213 4 -1.0456834039358114 2213 5 -0.8224587277335118 2213 6 -0.718082446482363 2213 7 -0.3822410249403916 2213 8 -0.2758506998395944 2213 9 -0.3613752824258114 2213 10 -0.2323696499618677 2213 11 -0.5111222142674521 2213 12 -0.3175982228540519 2213 13 -0.6674403280234529 2213 14 -0.34683258768423997 2213 15 -0.42260598566262075 2213 16 -1.6788514963033379 2213 17 -1.1259693899512229 2213 18 -0.406719646351803 2213 19 -0.34484387170258657 2213 20 -0.27997323407913655 2213 21 -0.12858588506288982 2213 22 -0.1999790133381012 2213 23 -0.030814863836937434 2213 24 -0.0312544410882154 2213 25 -0.017471416700956865 2214 1 -0.8802787102497405 2214 2 -0.7156893544437608 2214 3 -6.281474708653128 2214 4 -2.0501034350446474 2214 5 -1.2091262635523634 2214 6 -0.7733872347728408 2214 7 -0.5199500079120796 2214 8 -0.5008121621788533 2214 9 -0.1262832423578999 2214 10 -0.27228739946880154 2214 11 -0.7237825151685584 2214 12 -0.6559611034907107 2214 13 -5.2028489634839445 2214 14 -3.7551650836069896 2214 15 -2.103023269548629 2214 16 -2.870942567555602 2214 17 -3.5483922156630356 2214 18 -1.3033974256762306 2214 19 -0.8695631481922348 2214 20 -0.5414415397273373 2214 21 -0.4677936342668545 2214 22 -0.3606958842787549 2214 23 -0.33196396663601746 2214 24 -0.190562113890401 2214 25 -0.07556224031709746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1765.0 3 218.0 4 235.0 5 513.0 6 85.0 7 71.0 8 136.0 9 384.0 10 974.0 11 2482.0 12 5650.0 13 10695.0 14 16913.0 15 23765.0 16 29944.0 17 34106.0 18 36108.0 19 37186.0 20 36591.0 21 36723.0 22 37129.0 23 37954.0 24 39548.0 25 42684.0 26 46834.0 27 52574.0 28 61511.0 29 74729.0 30 92695.0 31 115537.0 32 150938.0 33 200757.0 34 271355.0 35 371715.0 36 523135.0 37 793383.0 38 1533270.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23564801050467 21.379488330055572 13.071308999159815 22.313554660279948 2 14.763639415884494 22.134140370347918 41.32445100866203 21.777769205105564 3 17.875961118193214 28.68379004773955 29.899556604281507 23.54069222978573 4 11.266698983462264 17.862801425302102 39.68719404935737 31.183305541878266 5 11.921594477361868 39.818907659650684 35.19291399915431 13.066583863833142 6 28.945208862658056 39.96580522546186 18.335098190729678 12.753887721150411 7 24.56640971878187 34.60346694156974 24.063473610640326 16.766649729008062 8 23.506509418603862 37.91284823736103 21.792307405322173 16.788334938712936 9 24.690283684034238 17.098078953281806 22.383204514523385 35.82843284816057 10 14.166388430461618 31.184289882907606 34.12723397173968 20.522087714891097 11 30.89919853753078 27.303989972657135 23.30204063549748 18.494770854314606 12 19.746184801613076 29.498173588770875 32.38125783262476 18.374383776991287 13 25.933883025640885 25.409974547565866 27.140896933858222 21.515245492935026 14 19.761573360591623 24.36187581905234 29.18582116793737 26.69072965241867 15 22.506149287725613 31.386859147551743 24.958974716638828 21.148016848083813 16 20.519159246497303 32.36072145138195 26.570032231498686 20.550087070622062 17 18.59996972843352 31.19573959499697 29.40331520289793 20.80097547367158 18 18.98912868452691 30.66452223217216 30.547286923341133 19.799062159959792 19 19.852437059018815 29.51328778780243 30.902337441419807 19.73193771175895 20 21.902718180943268 28.843124926600677 29.679824206238 19.574332686218053 21 22.166243167108256 29.460561122252987 28.685267578446695 19.687928132192063 22 20.507129366937317 29.138432101050228 30.243054164363915 20.111384367648547 23 20.231580134502263 29.644661162322745 29.857327710841332 20.26643099233366 24 20.273625485743434 28.85883658179942 30.07628787600925 20.791250056447897 25 20.798115437108947 28.940026652183075 29.142016789264165 21.119841121443812 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4282.0 1 4282.0 2 4694.5 3 5107.0 4 5107.0 5 5107.0 6 8915.5 7 12724.0 8 12724.0 9 12724.0 10 20159.0 11 27594.0 12 27594.0 13 27594.0 14 43957.5 15 60321.0 16 60321.0 17 60321.0 18 91841.0 19 123361.0 20 123361.0 21 123361.0 22 169888.0 23 216415.0 24 216415.0 25 216415.0 26 273716.0 27 331017.0 28 331017.0 29 331017.0 30 386783.0 31 442549.0 32 442549.0 33 442549.0 34 483778.5 35 525008.0 36 525008.0 37 525008.0 38 543476.0 39 561944.0 40 561944.0 41 561944.0 42 549541.5 43 537139.0 44 537139.0 45 537139.0 46 539959.5 47 542780.0 48 542780.0 49 542780.0 50 501745.5 51 460711.0 52 460711.0 53 460711.0 54 405537.0 55 350363.0 56 350363.0 57 350363.0 58 297345.5 59 244328.0 60 244328.0 61 244328.0 62 198229.5 63 152131.0 64 152131.0 65 152131.0 66 113621.0 67 75111.0 68 75111.0 69 75111.0 70 53177.5 71 31244.0 72 31244.0 73 31244.0 74 21292.0 75 11340.0 76 11340.0 77 11340.0 78 7429.0 79 3518.0 80 3518.0 81 3518.0 82 2245.5 83 973.0 84 973.0 85 973.0 86 599.5 87 226.0 88 226.0 89 226.0 90 144.5 91 63.0 92 63.0 93 63.0 94 39.5 95 16.0 96 16.0 97 16.0 98 21.5 99 27.0 100 27.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09901929795868561 2 0.05207304971811066 3 0.055293189489124824 4 0.051162089124994814 5 0.047814838573545876 6 0.0677288608416598 7 0.06340709430687762 8 0.05974206680434176 9 0.0650807195826021 10 0.0651866452329644 11 0.05912769803224038 12 0.060250509926080845 13 0.06069539765760254 14 0.06249613371376178 15 0.06522901549310933 16 0.06052591661702284 17 0.06342827943695009 18 0.14183444583513055 19 0.06893641325579011 20 0.06241139319347193 21 0.060441176096732996 22 0.06594930991557302 23 0.06457227646086301 24 0.07527076714745613 25 0.06842797013405102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4720292.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.45878837114638 #Duplication Level Percentage of deduplicated Percentage of total 1 88.37988690297965 68.45798955883751 2 8.815953360068804 13.657461312149326 3 1.596051820034216 3.7088472047224 4 0.464773534956039 1.440031795386777 5 0.21362715902370996 0.8273650450573391 6 0.12279164832363654 0.5706775380746876 7 0.06504466180818641 0.35267964855711686 8 0.05107632565224359 0.3165048239578313 9 0.035421008328173145 0.2469301549186119 >10 0.21706790308501728 3.1359482460408983 >50 0.021632280329463835 1.156384633486157 >100 0.013940638538463042 2.1322683451943036 >500 0.0014353884705435138 0.7631672320226235 >1k 0.0011041433207581912 1.6788676028642493 >5k 8.281074905634248E-5 0.45672810576496087 >10k+ 1.104143320751233E-4 1.0981487529651417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 15411 0.326484039546706 No Hit GTACATGGGAAGCAGTGGTATCAAC 13265 0.2810207504112034 No Hit CCCATGTACTCTGCGTTGATACCAC 12017 0.25458170808077124 No Hit GGTATCAACGCAGAGTACTTTTTTT 10667 0.22598178248294812 No Hit TATCAACGCAGAGTACTTTTTTTTT 9463 0.2004748858757043 No Hit GAGTACATGGGAAGCAGTGGTATCA 6185 0.1310300294981751 No Hit ATTCCATTCCATTCCATTCCATTCC 5713 0.12103064810397324 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.355539021738485E-5 0.0 5 0.0 0.0 0.0 6.355539021738485E-5 0.0 6 0.0 0.0 0.0 6.355539021738485E-5 0.0 7 0.0 0.0 0.0 6.355539021738485E-5 0.0 8 0.0 0.0 0.0 8.474052028984648E-5 0.0 9 0.0 0.0 0.0 1.271107804347697E-4 0.0 10 0.0 0.0 0.0 1.4829591050723133E-4 0.0 11 0.0 0.0 0.0 1.4829591050723133E-4 0.0 12 0.0 0.0 0.0 1.4829591050723133E-4 0.0 13 0.0 0.0 0.0 1.6948104057969295E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCAG 75 1.4842342E-5 12.663225 5 TACACCG 60 0.005890565 11.080322 5 CGTCGGT 70 0.0014953677 10.854538 19 TGTCCCG 190 9.094947E-12 10.496145 11 CCCGTAC 185 6.002665E-11 10.274777 16 CCCCGTA 185 6.002665E-11 10.274669 15 GTCTTGC 910 0.0 9.605044 1 AGCGTCG 80 0.0045020026 9.504169 17 TCGCGCG 110 6.864582E-5 9.497217 9 CCGTACC 145 1.7152088E-6 9.169727 9 TAGGACA 460 0.0 9.084487 4 GTCCCGC 200 2.6411726E-9 9.028673 12 TTCCGTA 190 1.0648364E-8 8.997459 7 ACCGCTC 85 0.0074573327 8.938558 8 CCGTACA 225 2.6011548E-10 8.870557 17 CGGTGAA 195 1.6600097E-8 8.766663 9 TATCGCG 120 1.7706057E-4 8.705875 7 GCGCGCC 120 1.7722347E-4 8.705137 11 CGCCCTA 230 4.0199666E-10 8.67772 16 CCCGCCC 230 4.0199666E-10 8.677628 14 >>END_MODULE