FastQCFastQC Report
Wed 25 May 2016
SRR1294648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4720292
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT390800.8279148832318001No Hit
GGTATCAACGCAGAGTACTTTTTTT297400.6300457683550085No Hit
TATCAACGCAGAGTACTTTTTTTTT253470.5369794919466846No Hit
ACGCAGAGTACTTTTTTTTTTTTTT149360.31642110276228674No Hit
CCCATGTACTCTGCGTTGATACCAC135970.2880542135952606No Hit
GTACATGGGAAGCAGTGGTATCAAC130320.2760846151043198No Hit
GTACTTTTTTTTTTTTTTTTTTTTT76350.16174846810324445No Hit
GAGTACTTTTTTTTTTTTTTTTTTT74210.15721485026773768No Hit
GAGTACATGGGAAGCAGTGGTATCA62790.13302143172498648No Hit
ATTCCATTCCATTCCATTCCATTCC54270.1149717009032492No Hit
CATGTACTCTGCGTTGATACCACTG51760.10965423325506134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG550.0030705912.0906182
ACCGTTC600.00578516411.1072318
TATTCCG1657.8216544E-1110.9629815
CGAACCG750.002689722210.11484112
CGCCCTA1701.6134436E-910.04046816
GGTATCA112350.09.8679281
TAGACAG7100.09.7886675
TGTCCCG1752.6284397E-99.76052511
GCCTAAC2350.09.710551
ATACCGA800.00443875149.5204846
ATACCGT1356.670067E-69.1678736
TATACTG9850.08.9888845
GAACCGG950.00185564668.98348113
ACATCGC850.00730915048.9604558
TATACCG850.00730915048.9604555
AACCGGG850.00755237238.92476714
ACCGGGG850.00755302848.92467115
GGAACGA1701.7358798E-78.92467115
CCTATAC4700.08.8934033
TTAGACA7000.08.8404494
Produced by FastQC (version 0.11.5)