##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294648_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4720292 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27881876799147 31.0 31.0 34.0 28.0 34.0 2 31.42264376864821 31.0 31.0 34.0 28.0 34.0 3 31.569434899366396 31.0 31.0 34.0 28.0 34.0 4 35.1408605654057 37.0 35.0 37.0 32.0 37.0 5 35.26506877116924 37.0 35.0 37.0 32.0 37.0 6 34.88404191944058 37.0 35.0 37.0 32.0 37.0 7 34.72203499275045 37.0 35.0 37.0 32.0 37.0 8 34.77607762401139 37.0 35.0 37.0 32.0 37.0 9 36.196597583369844 39.0 37.0 39.0 31.0 39.0 10 35.99006226733431 39.0 35.0 39.0 30.0 39.0 11 36.14154653991744 39.0 35.0 39.0 31.0 39.0 12 36.082056364309665 39.0 35.0 39.0 30.0 39.0 13 35.93647299785691 39.0 35.0 39.0 30.0 39.0 14 37.0235936675104 40.0 36.0 41.0 30.0 41.0 15 37.07150468657448 40.0 36.0 41.0 30.0 41.0 16 37.112997246780495 40.0 36.0 41.0 31.0 41.0 17 37.173385460052046 40.0 37.0 41.0 31.0 41.0 18 37.19374373449778 40.0 37.0 41.0 31.0 41.0 19 37.22536953222385 40.0 37.0 41.0 30.0 41.0 20 37.281681514618164 40.0 37.0 41.0 31.0 41.0 21 37.13254031742104 40.0 37.0 41.0 30.0 41.0 22 37.12716437881385 40.0 37.0 41.0 30.0 41.0 23 37.139598143504685 40.0 37.0 41.0 30.0 41.0 24 37.06308359737067 40.0 36.0 41.0 30.0 41.0 25 36.010439396545806 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5134879880463501 1101 2 0.5196784287880192 1101 3 0.47085859101763106 1101 4 0.3649660593417181 1101 5 0.4316245297102199 1101 6 0.4552135240414401 1101 7 0.561700762877166 1101 8 0.5918757012251277 1101 9 0.7589971657662957 1101 10 0.7835511214985047 1101 11 0.7781089624204753 1101 12 0.8548925016009221 1101 13 0.8273453982671413 1101 14 1.0401689218213193 1101 15 0.8943639530179723 1101 16 0.9591753122005358 1101 17 0.9409386249511371 1101 18 0.9487724501897077 1101 19 1.086183829146961 1101 20 0.9992239450651965 1101 21 1.2996087670295537 1101 22 1.2187842947167837 1101 23 1.1705796062336589 1101 24 1.2881043565431014 1101 25 1.2141043485598715 1102 1 0.3909355507728449 1102 2 0.40700314460298515 1102 3 0.37459696896803507 1102 4 0.2900270050837719 1102 5 0.3502974698206103 1102 6 0.43316954398901686 1102 7 0.4575711910278315 1102 8 0.4742251181164505 1102 9 0.6735328667719358 1102 10 0.7916938311701287 1102 11 0.711235101087361 1102 12 0.7681924535713307 1102 13 0.7342503179436974 1102 14 0.9088069371860215 1102 15 0.8574665277856326 1102 16 0.9303020620628786 1102 17 0.809057288043995 1102 18 0.8235601109140518 1102 19 0.9344460635838203 1102 20 0.8645986096724272 1102 21 1.1854846496577807 1102 22 1.1731235292545534 1102 23 1.0142529088502954 1102 24 1.1867884042421721 1102 25 1.0032776426352683 1103 1 0.33844587602264653 1103 2 0.3364277323515523 1103 3 0.29255552738938206 1103 4 0.2774694162763751 1103 5 0.2422754792353956 1103 6 0.3573186221346347 1103 7 0.3717950230559879 1103 8 0.42460960092398636 1103 9 0.33516574429423684 1103 10 0.5328362795222077 1103 11 0.5430165273411802 1103 12 0.64885610823886 1103 13 0.6922645973474602 1103 14 0.8155435839121452 1103 15 0.735202827348509 1103 16 0.7654541316838888 1103 17 0.7550038393849476 1103 18 0.8007939744233283 1103 19 0.8391357622926208 1103 20 0.7268216179267171 1103 21 1.0215928589850023 1103 22 0.932168725397851 1103 23 0.9736456943171987 1103 24 1.0551179367421284 1103 25 0.9629627806395575 1104 1 0.14832453199471374 1104 2 0.1922807981997856 1104 3 0.06420752545871622 1104 4 0.0737170138753882 1104 5 0.13423054076182694 1104 6 0.12058878130024198 1104 7 0.13030878397189127 1104 8 0.1097645077004259 1104 9 -0.08457238722370874 1104 10 0.3726813112700498 1104 11 0.3886535633323476 1104 12 0.35961919619743554 1104 13 -0.09981026048671282 1104 14 0.23900867360963218 1104 15 0.47013818259579665 1104 16 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2 0.3071411818109553 2207 3 0.2795473678425182 2207 4 0.26375886818351546 2207 5 0.22851341180133744 2207 6 0.4187062642753645 2207 7 0.40444036305665776 2207 8 0.37213186653319497 2207 9 0.4649821807996233 2207 10 0.5948259171459824 2207 11 0.5583269611058199 2207 12 0.514687335114786 2207 13 0.5668509047052979 2207 14 0.5586396907555553 2207 15 0.6165283234205887 2207 16 0.6356746284939945 2207 17 0.67099537850671 2207 18 0.6276385573577556 2207 19 0.7171004788369189 2207 20 0.6454306111667947 2207 21 0.9634606983775029 2207 22 0.8117166929891084 2207 23 0.7717732372652293 2207 24 0.9161164129472823 2207 25 0.9232371894375575 2208 1 0.16367598764653124 2208 2 0.18087075728334057 2208 3 0.17029905282872448 2208 4 0.16492640427077276 2208 5 0.16107832197743477 2208 6 0.26476890148391163 2208 7 0.24493811132146703 2208 8 0.19303170025733607 2208 9 0.29198394927762905 2208 10 0.3913162756675206 2208 11 -0.023252458286663114 2208 12 -0.1930071808782614 2208 13 0.012637056876677377 2208 14 -0.42388655884578696 2208 15 0.1126246227542893 2208 16 0.25051109957088613 2208 17 0.172744117061697 2208 18 0.39801736906262164 2208 19 0.48815144931023724 2208 20 0.4114633262405718 2208 21 0.7163487185093089 2208 22 0.651739853946701 2208 23 0.6570699549647401 2208 24 0.7637815287675522 2208 25 0.7418801568180342 2209 1 0.048183202119037816 2209 2 0.06120131085819125 2209 3 0.13189077938042715 2209 4 -0.4634549839109283 2209 5 0.04212881079448749 2209 6 0.17550672663698919 2209 7 0.13751699763287917 2209 8 0.18919493451831215 2209 9 0.3444581322780067 2209 10 0.3657978867937999 2209 11 0.3570951875597217 2209 12 0.38571701691350313 2209 13 0.4375883548593933 2209 14 0.40992812280189384 2209 15 -0.22398671608333842 2209 16 -0.26332466692765166 2209 17 0.14332267511384345 2209 18 -0.14578330787897897 2209 19 0.26771018821614234 2209 20 0.378454413716625 2209 21 0.6370501765677261 2209 22 0.5435288581816451 2209 23 0.4696042107255991 2209 24 0.5778459372675826 2209 25 0.6171599660682787 2210 1 -0.11875932779874887 2210 2 -0.1253539305642981 2210 3 -0.12045308778771613 2210 4 -0.02199821658596335 2210 5 -0.013507231681934684 2210 6 -0.4087079701017515 2210 7 -0.15325635744239463 2210 8 -0.29528520331670194 2210 9 -0.0498990657835634 2210 10 0.09415990460584567 2210 11 0.05563867669958 2210 12 0.11396701673358933 2210 13 0.10794237947404639 2210 14 0.13760767375116956 2210 15 0.04262757592947253 2210 16 0.1470752893163052 2210 17 -0.9231912815777505 2210 18 -1.4854881892660714 2210 19 -0.3364259373823586 2210 20 -0.2229137454466681 2210 21 -0.37542960692711347 2210 22 -0.35393642752660526 2210 23 -0.14771686760170155 2210 24 0.1537048638170333 2210 25 0.23507646369387913 2211 1 -0.25753866605180775 2211 2 -0.2509685882888988 2211 3 -0.18562315369319649 2211 4 -0.1519242675128467 2211 5 -0.16837601304749938 2211 6 -0.14229165342713657 2211 7 -0.2339369466383161 2211 8 -0.16644360108769263 2211 9 -0.18386093477531773 2211 10 -0.13747594333522528 2211 11 -0.513088695565294 2211 12 -0.3289264825385203 2211 13 -0.2588908087189452 2211 14 -0.3575727366526422 2211 15 -0.35999050415242806 2211 16 -0.2743258799594628 2211 17 -0.43069117966488335 2211 18 -0.49581873442742364 2211 19 -0.3215167000271464 2211 20 -0.48889758220222035 2211 21 -0.12889573888635653 2211 22 -0.2855868852590291 2211 23 -0.9407161647448845 2211 24 -0.278802414403458 2211 25 -0.8908245076383778 2212 1 -0.21152416838984323 2212 2 -0.2666084206750199 2212 3 -0.18943001213454735 2212 4 -0.07364308724193336 2212 5 -0.041224761803974275 2212 6 -0.05170944002944822 2212 7 -0.17100507418365396 2212 8 -0.2797161809674762 2212 9 -0.10378914344961032 2212 10 -0.4884366917430114 2212 11 -0.18734041441643257 2212 12 -0.2828700676637439 2212 13 -0.21937009228997084 2212 14 -0.31962411203787156 2212 15 -0.17816163554217468 2212 16 -0.3269488176473203 2212 17 -0.547309135977919 2212 18 -0.39413326980506014 2212 19 -0.4101825998149664 2212 20 -0.5494592669639644 2212 21 -0.04696182201045218 2212 22 -0.3173450639747841 2212 23 -0.22647444319807875 2212 24 -0.43992869555349756 2212 25 -0.15134234130184154 2213 1 -0.23726357837643164 2213 2 -0.3193073090437224 2213 3 -0.20562279351486623 2213 4 -0.1313542961312777 2213 5 -0.10293345573412438 2213 6 -0.380275228508026 2213 7 -0.4230922969643913 2213 8 -0.3633452457347701 2213 9 -0.3617763593926071 2213 10 -0.3458787413894342 2213 11 -0.1387687166800049 2213 12 -0.05856388432226822 2213 13 -0.016357226764156962 2213 14 -0.7016881952605232 2213 15 -0.39500997395614945 2213 16 -0.3090024176773696 2213 17 -0.3153252980196797 2213 18 -0.313478848702367 2213 19 -0.45528122389846004 2213 20 -0.45519192682183274 2213 21 -0.20565301303707884 2213 22 -0.4754266236160447 2213 23 -0.5597433181561513 2213 24 -0.7413739813947089 2213 25 -0.5235745292146348 2214 1 -0.28634626411654907 2214 2 -0.160419167329799 2214 3 -0.22172902476312828 2214 4 -0.09828883612035355 2214 5 -0.2477182613436426 2214 6 -0.17548300000854056 2214 7 -0.2531194971955273 2214 8 -0.1906531196187231 2214 9 -0.22751404998609104 2214 10 -0.2273988988814608 2214 11 -0.12887506550123362 2214 12 -0.31546694747985526 2214 13 -0.3621656810033187 2214 14 -0.2898396071450904 2214 15 -0.19385453315896228 2214 16 -0.4323858733242574 2214 17 -0.4811468896695814 2214 18 -0.267058560873636 2214 19 -0.48532416073891227 2214 20 -0.42823360282388023 2214 21 -0.5775772710693658 2214 22 -0.8041188958983057 2214 23 -0.9342912906341709 2214 24 -0.5408495533409763 2214 25 -0.9613123944643505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 17.0 9 81.0 10 169.0 11 344.0 12 817.0 13 2092.0 14 4352.0 15 7416.0 16 11193.0 17 14615.0 18 17499.0 19 20024.0 20 21385.0 21 22886.0 22 24769.0 23 26737.0 24 29529.0 25 32712.0 26 37545.0 27 42100.0 28 49275.0 29 61324.0 30 78734.0 31 101644.0 32 137633.0 33 186082.0 34 256053.0 35 358402.0 36 515866.0 37 824569.0 38 1834427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.999427870914275 21.793771002894786 12.199730306237502 24.007070819953434 2 14.537109144942729 22.341096694865488 40.06078437520391 23.061009784987878 3 17.324016743218326 28.280973299267632 30.634120119778668 23.760889837735373 4 11.224392073275379 17.848160805808806 39.59783716441407 31.32960995650175 5 11.979853356438955 39.127750096520636 35.35032859396872 13.542067953071687 6 29.122942247323564 40.16906116305402 17.646881843637917 13.0611147459845 7 24.395953671487963 34.78115583958774 23.88509834002212 16.93779214890218 8 23.921277714705134 37.60529352510242 21.368742724221125 17.104686035971323 9 24.74544428798019 15.997261181162232 22.704168372955603 36.55312615790197 10 14.244114762790206 30.81875985356798 34.09944370208532 20.837681681556504 11 31.265019477623202 26.368839364301934 23.266326182775913 19.099814975298948 12 20.173618695194662 28.952281764295755 32.52933279296407 18.34476674754551 13 26.504981685447294 24.441888104332573 27.508373426208983 21.544756784011152 14 20.033422050630083 23.579896517907894 29.84350150214915 26.54317992931287 15 22.780468200670263 30.50558223616125 25.594561803094372 21.119387760074115 16 20.38272640394416 31.741602371548705 27.15239378250197 20.723277442005163 17 18.423854646157185 30.77473238921015 29.931669423560596 20.86974354107207 18 18.726782347730865 29.798611395051605 31.574545307404627 19.900060949812904 19 19.48863453400064 28.719156386168233 32.31471799034969 19.477491089481436 20 21.452849639068177 27.975680266560342 31.21057174126461 19.36089835310687 21 21.744519762497088 28.724806309118595 30.025174359396978 19.505499568987343 22 20.025634724246636 28.482953967662112 31.596683163282602 19.894728144808653 23 19.818379802935837 28.91277866402276 31.26778227608897 20.001059256952434 24 19.795029586099673 28.2258657358201 31.541190998605806 20.437913679474423 25 20.337383365863673 28.247011943750795 30.58932348122721 20.826281209158324 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2825.0 1 2825.0 2 3502.5 3 4180.0 4 4180.0 5 4180.0 6 9675.0 7 15170.0 8 15170.0 9 15170.0 10 21365.0 11 27560.0 12 27560.0 13 27560.0 14 39713.5 15 51867.0 16 51867.0 17 51867.0 18 83029.5 19 114192.0 20 114192.0 21 114192.0 22 164204.5 23 214217.0 24 214217.0 25 214217.0 26 275436.5 27 336656.0 28 336656.0 29 336656.0 30 394580.0 31 452504.0 32 452504.0 33 452504.0 34 492339.5 35 532175.0 36 532175.0 37 532175.0 38 544448.5 39 556722.0 40 556722.0 41 556722.0 42 544053.5 43 531385.0 44 531385.0 45 531385.0 46 537194.0 47 543003.0 48 543003.0 49 543003.0 50 503814.5 51 464626.0 52 464626.0 53 464626.0 54 407849.0 55 351072.0 56 351072.0 57 351072.0 58 298804.0 59 246536.0 60 246536.0 61 246536.0 62 200102.0 63 153668.0 64 153668.0 65 153668.0 66 114563.5 67 75459.0 68 75459.0 69 75459.0 70 53228.0 71 30997.0 72 30997.0 73 30997.0 74 21091.0 75 11185.0 76 11185.0 77 11185.0 78 7195.5 79 3206.0 80 3206.0 81 3206.0 82 2032.5 83 859.0 84 859.0 85 859.0 86 518.5 87 178.0 88 178.0 89 178.0 90 108.5 91 39.0 92 39.0 93 39.0 94 24.5 95 10.0 96 10.0 97 10.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09687959982136699 2 0.0 3 0.1327036547738996 4 0.01190604310072343 5 0.023176532299273012 6 0.01228737544202774 7 0.02016824382898346 8 0.01834632264275176 9 1.9066617065215455E-4 10 0.38800565727713454 11 0.06533494114347163 12 0.001334663194565082 13 4.237026014492324E-5 14 0.0011651821539853892 15 0.0010804416336955426 16 2.118513007246162E-5 17 8.474052028984648E-5 18 3.177769510869243E-4 19 5.931836420289253E-4 20 2.1185130072461618E-4 21 2.7540669094200105E-4 22 0.002796437169564934 23 4.237026014492324E-5 24 2.118513007246162E-5 25 0.0017583657960143144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4720292.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.56069149387406 #Duplication Level Percentage of deduplicated Percentage of total 1 87.43830504116599 65.1946048692163 2 9.602983807845206 14.320102262348286 3 1.676058936782158 3.749043399328951 4 0.5077617960251959 1.5143628250324015 5 0.20829187077930894 0.7765192958928967 6 0.12143019206987446 0.5432351453378268 7 0.08338520591188585 0.43520810292044987 8 0.05194731069613124 0.3098581925400532 9 0.0375458461467178 0.2519499826279697 >10 0.2296849842213846 3.222837416034058 >50 0.023875612428519415 1.225737199087437 >100 0.015844988521738507 2.332644572159875 >500 0.00137080807393237 0.7142449307204651 >1k 0.0011994560743811874 1.8393876554232969 >5k 1.4279238980680016E-4 0.6800685660235181 >10k+ 1.7135086776816016E-4 2.890195585306035 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 39080 0.8279148832318001 No Hit GGTATCAACGCAGAGTACTTTTTTT 29740 0.6300457683550085 No Hit TATCAACGCAGAGTACTTTTTTTTT 25347 0.5369794919466846 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 14936 0.31642110276228674 No Hit CCCATGTACTCTGCGTTGATACCAC 13597 0.2880542135952606 No Hit GTACATGGGAAGCAGTGGTATCAAC 13032 0.2760846151043198 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 7635 0.16174846810324445 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 7421 0.15721485026773768 No Hit GAGTACATGGGAAGCAGTGGTATCA 6279 0.13302143172498648 No Hit ATTCCATTCCATTCCATTCCATTCC 5427 0.1149717009032492 No Hit CATGTACTCTGCGTTGATACCACTG 5176 0.10965423325506134 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.118513007246162E-5 0.0 3 0.0 0.0 0.0 2.118513007246162E-5 0.0 4 0.0 0.0 0.0 2.118513007246162E-5 0.0 5 0.0 0.0 0.0 2.118513007246162E-5 0.0 6 0.0 0.0 0.0 2.118513007246162E-5 0.0 7 0.0 0.0 0.0 4.237026014492324E-5 0.0 8 0.0 0.0 0.0 4.237026014492324E-5 0.0 9 0.0 0.0 0.0 6.355539021738485E-5 0.0 10 0.0 0.0 0.0 8.474052028984648E-5 0.0 11 0.0 0.0 0.0 8.474052028984648E-5 0.0 12 0.0 0.0 0.0 1.0592565036230809E-4 0.0 13 0.0 0.0 0.0 1.4829591050723133E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTAAG 55 0.00307059 12.090618 2 ACCGTTC 60 0.005785164 11.107231 8 TATTCCG 165 7.8216544E-11 10.962981 5 CGAACCG 75 0.0026897222 10.114841 12 CGCCCTA 170 1.6134436E-9 10.040468 16 GGTATCA 11235 0.0 9.867928 1 TAGACAG 710 0.0 9.788667 5 TGTCCCG 175 2.6284397E-9 9.760525 11 GCCTAAC 235 0.0 9.71055 1 ATACCGA 80 0.0044387514 9.520484 6 ATACCGT 135 6.670067E-6 9.167873 6 TATACTG 985 0.0 8.988884 5 GAACCGG 95 0.0018556466 8.983481 13 ACATCGC 85 0.0073091504 8.960455 8 TATACCG 85 0.0073091504 8.960455 5 AACCGGG 85 0.0075523723 8.924767 14 ACCGGGG 85 0.0075530284 8.924671 15 GGAACGA 170 1.7358798E-7 8.924671 15 CCTATAC 470 0.0 8.893403 3 TTAGACA 700 0.0 8.840449 4 >>END_MODULE