FastQCFastQC Report
Wed 25 May 2016
SRR1294647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4740102
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC105750.22309646501277822No Hit
CCCATGTACTCTGCGTTGATACCAC103050.21740038505500514No Hit
GTATCAACGCAGAGTACTTTTTTTT98450.20769595253435474No Hit
GGTATCAACGCAGAGTACTTTTTTT56210.11858394608386064No Hit
TATCAACGCAGAGTACTTTTTTTTT53060.11193851946645875No Hit
GAGTACATGGGAAGCAGTGGTATCA48020.10130583687861569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG905.422353E-712.66482319
AGGACCG951.3656694E-510.9971215
CTATGCG803.7851E-410.6840269
GTCTTAG4900.010.27520751
GGACCGT856.616716E-410.0557676
CGAACCG1252.559913E-69.88398812
TAGGACC3350.09.6392674
CCCCGTA3000.09.50403515
CTGGTCG900.00111771839.4969129
GTCCTAA4000.09.2622271
CGCCCTA3200.09.20732716
AAATCGC1356.81436E-69.15242117
TAGACAG7200.09.1017845
CTAGGAC5150.09.0364693
GCGTCGG950.00181488619.00544118
AGCGTAC3800.08.9997351
TAACGTG850.00745543378.9388375
CCACGAC850.00745543378.9388373
GTATAGG5900.08.8556711
GTCTTAC4550.08.7689721
Produced by FastQC (version 0.11.5)