##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294647_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4740102 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.285626764993665 31.0 30.0 34.0 26.0 34.0 2 30.48119344267275 31.0 30.0 34.0 26.0 34.0 3 30.42542038124918 31.0 30.0 34.0 26.0 34.0 4 34.15186529741343 37.0 35.0 37.0 30.0 37.0 5 34.436717184566916 37.0 35.0 37.0 30.0 37.0 6 34.51760953667242 37.0 35.0 37.0 30.0 37.0 7 34.46341007007866 37.0 35.0 37.0 30.0 37.0 8 34.26712885081376 37.0 35.0 37.0 30.0 37.0 9 35.50559101892744 39.0 35.0 39.0 28.0 39.0 10 35.61618610738756 39.0 35.0 39.0 29.0 39.0 11 35.825012837276496 39.0 35.0 39.0 30.0 39.0 12 35.61166911598105 39.0 35.0 39.0 29.0 39.0 13 35.484217428232554 39.0 35.0 39.0 28.0 39.0 14 36.43477165681245 39.0 36.0 41.0 27.0 41.0 15 36.41970721305153 39.0 36.0 41.0 27.0 41.0 16 36.53802217758183 39.0 36.0 41.0 28.0 41.0 17 36.517597933546575 39.0 36.0 41.0 28.0 41.0 18 36.414210706858206 39.0 36.0 41.0 27.0 41.0 19 36.69317896534716 40.0 36.0 41.0 28.0 41.0 20 36.78744486932982 40.0 36.0 41.0 29.0 41.0 21 36.83554151366363 40.0 36.0 41.0 29.0 41.0 22 36.83485903889832 40.0 36.0 41.0 29.0 41.0 23 36.80748388958719 40.0 36.0 41.0 29.0 41.0 24 36.801695617520465 40.0 36.0 41.0 29.0 41.0 25 35.65475489767942 39.0 34.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.007048586028425 1101 2 1.0313837518402877 1101 3 1.2883913683263764 1101 4 1.0428460713317094 1101 5 0.8039209163122294 1101 6 0.810913933631916 1101 7 0.7914638438363752 1101 8 0.9658335122502848 1101 9 1.2250037456590306 1101 10 1.1920994034050238 1101 11 0.9370885311592403 1101 12 1.053121107075654 1101 13 1.1698545194574805 1101 14 1.307475819569376 1101 15 1.4120668622321872 1101 16 1.3910210968566332 1101 17 1.3655892140549923 1101 18 1.3481781062705167 1101 19 1.2037785635852885 1101 20 1.1832889183982758 1101 21 1.1258571339533887 1101 22 1.0405659732620123 1101 23 1.0741367915175744 1101 24 1.146126300143436 1101 25 1.0783511856858254 1102 1 0.79688553975517 1102 2 0.7669375019243532 1102 3 1.0443103258429645 1102 4 0.8768873956400967 1102 5 0.67755075795381 1102 6 0.6396952205957263 1102 7 0.5591495579525443 1102 8 0.6595252849705489 1102 9 0.9632888125746604 1102 10 0.8693471000277952 1102 11 0.6045999343105848 1102 12 0.8730614408032693 1102 13 0.9258613000303839 1102 14 1.0603782825709445 1102 15 1.1947937582409835 1102 16 1.045373480936135 1102 17 1.1108525546541657 1102 18 1.1421983748033568 1102 19 0.8659070463430965 1102 20 0.15399374787517672 1102 21 0.4604511105576421 1102 22 0.538847094074093 1102 23 0.704367603959966 1102 24 0.8062852915233236 1102 25 0.7562059295154597 1103 1 0.6937106631435341 1103 2 0.7394721627718503 1103 3 1.025773368285467 1103 4 0.7894028946876901 1103 5 0.5796665772747787 1103 6 0.5407763486267996 1103 7 0.44159926034183883 1103 8 0.6013386469616151 1103 9 0.6602009504913013 1103 10 0.6273825891018703 1103 11 0.5787268414303384 1103 12 0.7793028698178688 1103 13 0.697300819678297 1103 14 0.6805994901001711 1103 15 0.8979649020344596 1103 16 0.8546355571465085 1103 17 0.8893447932916629 1103 18 1.0015213962164466 1103 19 0.7247106020154774 1103 20 -0.0969613099605553 1103 21 0.2957538578151713 1103 22 -0.21778185945147044 1103 23 0.33960712283473526 1103 24 0.4866889867475379 1103 25 0.5554317261157706 1104 1 0.3814886505087216 1104 2 0.44954586634084137 1104 3 0.6910113668239717 1104 4 0.6322222404681028 1104 5 0.41527071699717766 1104 6 0.33701432881387205 1104 7 0.2670808215133391 1104 8 0.4382848841806677 1104 9 0.7195675492576612 1104 10 0.6699692507384398 1104 11 0.48573087363138967 1104 12 0.5748220273607174 1104 13 0.5340433620016682 1104 14 0.7842517924078507 1104 15 0.8564507342764784 1104 16 0.6801034841053948 1104 17 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-1.2378841607582771 2207 10 -0.18946472108683565 2207 11 0.1160727271007147 2207 12 0.49627580579571884 2207 13 0.5763485436447908 2207 14 0.7548817158440997 2207 15 0.8381244031635902 2207 16 0.7239995057385684 2207 17 0.7333549224287523 2207 18 0.800215696554325 2207 19 -0.3511216925209695 2207 20 0.21807701214841302 2207 21 -0.3537757000499866 2207 22 0.21897092128574513 2207 23 -0.49601364474515464 2207 24 -1.651001904735594 2207 25 -0.3046007416894412 2208 1 0.5292019462809279 2208 2 0.15745303073473949 2208 3 0.6379906484744069 2208 4 0.5400257032368927 2208 5 0.4048767308349568 2208 6 0.2824913793918 2208 7 0.3792601502701416 2208 8 0.47262349894919 2208 9 -0.15017465607538583 2208 10 0.09169978992040484 2208 11 0.23830328135841228 2208 12 0.26565491414146436 2208 13 0.5371698724622647 2208 14 0.835886715806474 2208 15 0.6791064966079503 2208 16 0.5715692753599555 2208 17 0.4275649639457484 2208 18 0.6304476186359125 2208 19 -1.5325678575860806 2208 20 -0.16865858072234374 2208 21 -0.314962179526475 2208 22 0.18757179119984357 2208 23 0.40189768322405683 2208 24 0.5916861462504812 2208 25 0.518095814158066 2209 1 0.17119149570202907 2209 2 -1.4218251029064213 2209 3 -0.17955824904894158 2209 4 0.11655035046796769 2209 5 0.1448056654315124 2209 6 0.16909169008998504 2209 7 0.1185704091631905 2209 8 0.3045836747071675 2209 9 -1.9773317795975416 2209 10 -0.35158409150290026 2209 11 -0.025238517215186107 2209 12 0.32886689306427286 2209 13 0.4590818729223045 2209 14 0.6234350122659933 2209 15 0.7430566070734201 2209 16 0.6369883568273877 2209 17 0.43068365153620647 2209 18 0.726565859298411 2209 19 0.40065191469750516 2209 20 0.37293322393118444 2209 21 0.39190094169372713 2209 22 0.3928400037270592 2209 23 0.45578375917639136 2209 24 0.5101349828081752 2209 25 0.45184526461323316 2210 1 0.12483976511369832 2210 2 0.14174320612648117 2210 3 0.06678231682031566 2210 4 0.24741401446048883 2210 5 0.09795434478496645 2210 6 0.1594875420513091 2210 7 0.07471169252340104 2210 8 0.1999530226942241 2210 9 0.3663733352324101 2210 10 -1.4089039781533188 2210 11 -0.46679496876699034 2210 12 -2.3436016501465673 2210 13 -0.7696268285897432 2210 14 -0.42170202392947687 2210 15 -0.6770201869635457 2210 16 -0.1112317107314027 2210 17 0.14879357491322054 2210 18 0.38314614018531756 2210 19 0.27518086152818455 2210 20 0.2466057475253436 2210 21 0.26826794639898566 2210 22 0.1969129091280024 2210 23 0.08085892840548325 2210 24 0.16341349440904196 2210 25 0.0481751883718502 2211 1 -0.2699393378256296 2211 2 -0.016655898109100065 2211 3 -1.4983581723853945 2211 4 -0.5124637201193423 2211 5 -0.3537759162621228 2211 6 -0.37313997826457523 2211 7 -0.40019666969044465 2211 8 -0.006005275129730592 2211 9 0.19726076526423242 2211 10 0.10098248979860358 2211 11 -0.1670871531794873 2211 12 -0.1639664738503157 2211 13 -0.1724414613468639 2211 14 -0.644751139564292 2211 15 -0.32240263060892005 2211 16 0.010663240019759712 2211 17 -0.08844289809980665 2211 18 -0.024193229171729058 2211 19 -0.0467273648431501 2211 20 -0.03455779796161096 2211 21 -0.12101845815379875 2211 22 -0.14116920926020526 2211 23 -0.5413905010850328 2211 24 -0.21655302669545762 2211 25 -0.07216244893722035 2212 1 -0.3087495171207486 2212 2 -0.23800584413577042 2212 3 -2.337699026866659 2212 4 -1.2516886839171235 2212 5 -0.5711245214929193 2212 6 -0.3758389838813372 2212 7 -0.2473111369892962 2212 8 -0.24086342469095712 2212 9 -0.8794602477147961 2212 10 -0.16513361740790344 2212 11 -0.29969511013451466 2212 12 0.08020139108000279 2212 13 -0.2773164507810719 2212 14 0.017423521864166958 2212 15 0.1264465882249013 2212 16 -1.1285785321655553 2212 17 -1.259147864639253 2212 18 -0.1964189188302896 2212 19 -0.03897007602152769 2212 20 -0.15369695663642347 2212 21 -0.05015947931420328 2212 22 -0.33513303635081826 2212 23 -0.0718009914564135 2212 24 -0.11489543873781827 2212 25 0.040131699782129715 2213 1 -3.2061788136061082 2213 2 -1.5062809424165877 2213 3 -2.4845118679157707 2213 4 -1.1368090412485827 2213 5 -0.8253010895853947 2213 6 -0.6681723465522253 2213 7 -0.46616968595368036 2213 8 -0.2943454260991629 2213 9 -0.46892183018267275 2213 10 -0.3577779581929761 2213 11 -0.636342854076311 2213 12 -0.29154324014689337 2213 13 -0.5988718547592455 2213 14 -0.33347457412832426 2213 15 -0.4752157511519641 2213 16 -1.67696680007613 2213 17 -1.2507353198994764 2213 18 -0.4675419605482958 2213 19 -0.32638646398139315 2213 20 -0.3364842446725689 2213 21 -0.17744964243102146 2213 22 -0.15117774678912355 2213 23 -0.10623925056572858 2213 24 -0.093700153283379 2213 25 -0.14157779971912987 2214 1 -0.9232362685851285 2214 2 -0.8356690364380519 2214 3 -6.52391093652728 2214 4 -2.2074212375601903 2214 5 -1.295915995672324 2214 6 -0.785903374253877 2214 7 -0.7348347151418295 2214 8 -0.42576710972127785 2214 9 -0.4201101634385864 2214 10 -0.36467917526920957 2214 11 -0.8573836787770688 2214 12 -0.5981387365003101 2214 13 -5.185499016772837 2214 14 -3.8294254923550426 2214 15 -2.328412995697576 2214 16 -3.228702314391491 2214 17 -3.6639052150698816 2214 18 -1.338996413096261 2214 19 -0.8636952312658863 2214 20 -0.512703933900049 2214 21 -0.45998097904706015 2214 22 -0.5263236875456911 2214 23 -0.46817435918900685 2214 24 -0.42805497513501223 2214 25 -0.3320041635103337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1858.0 3 250.0 4 212.0 5 573.0 6 73.0 7 76.0 8 110.0 9 308.0 10 843.0 11 1942.0 12 4151.0 13 7616.0 14 11828.0 15 16666.0 16 21202.0 17 23871.0 18 25920.0 19 26957.0 20 27570.0 21 28499.0 22 29643.0 23 31194.0 24 33851.0 25 36875.0 26 41721.0 27 48626.0 28 57361.0 29 71212.0 30 88274.0 31 112096.0 32 149093.0 33 200024.0 34 272151.0 35 377449.0 36 534353.0 37 811083.0 38 1644571.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32639645621202 21.17920575629024 13.089892196266655 23.404505591231086 2 14.724591610360719 21.530107588163403 41.094224603219836 22.651076198256046 3 18.059718694694748 27.08540903932668 29.923961612245176 24.930910653733402 4 11.74596701507401 17.437169106953412 39.37239396330346 31.44446991466912 5 12.48957829837622 38.85258912006012 35.52631967765018 13.131512903913494 6 30.0499918720985 39.42652904827551 17.898673568697657 12.624805510928333 7 25.59081896835624 33.66853255151379 23.75655058155147 16.984097898578504 8 23.69491874629787 37.90761425991812 21.576846610759493 16.82062038302452 9 24.46224658704891 17.19286712380272 22.184757360483676 36.16012892866469 10 14.328005623966881 30.499549280432014 34.058671654489146 21.113773441111956 11 32.091994970839146 26.44427941396259 23.38979880823411 18.073926806964156 12 20.444151252078 27.75818666385202 32.32884924928094 19.468812834789034 13 26.23656513227252 24.174486828900466 27.294297751753493 22.294650287073523 14 19.847476662346054 23.272464734915495 28.853826699711632 28.026231903026822 15 22.497021765652274 30.868546657527013 24.591021871629497 22.043409705191216 16 21.214485535827528 31.61398446808205 26.19014144566573 20.981388550424693 17 19.631523404341557 30.400490778585347 29.146100571188754 20.821885245884335 18 20.06257955811902 30.088978328500428 29.726600009507358 20.121842103873195 19 20.55984498925835 29.200178014078986 29.924014673494483 20.315962323168176 20 21.93632951053132 28.72748528925606 28.93502109134573 20.401164108866894 21 22.03020051347459 29.218887015746635 28.37685961439164 20.374052856387127 22 20.911066707622027 28.832487309644673 29.443672133362465 20.81277384937084 23 20.825518799214677 29.20293863075007 29.121957398298466 20.84958517173679 24 20.865080102355353 28.5791227166395 29.193465978667522 21.36233120233762 25 21.340169033257535 28.562938635983144 28.549194893238987 21.547697437520334 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3604.0 1 3604.0 2 3506.0 3 3408.0 4 3408.0 5 3408.0 6 5715.5 7 8023.0 8 8023.0 9 8023.0 10 13089.0 11 18155.0 12 18155.0 13 18155.0 14 31092.0 15 44029.0 16 44029.0 17 44029.0 18 72003.0 19 99977.0 20 99977.0 21 99977.0 22 144203.5 23 188430.0 24 188430.0 25 188430.0 26 247620.0 27 306810.0 28 306810.0 29 306810.0 30 367619.0 31 428428.0 32 428428.0 33 428428.0 34 475459.5 35 522491.0 36 522491.0 37 522491.0 38 549610.0 39 576729.0 40 576729.0 41 576729.0 42 568777.5 43 560826.0 44 560826.0 45 560826.0 46 558243.0 47 555660.0 48 555660.0 49 555660.0 50 515343.5 51 475027.0 52 475027.0 53 475027.0 54 420884.0 55 366741.0 56 366741.0 57 366741.0 58 312739.5 59 258738.0 60 258738.0 61 258738.0 62 213515.0 63 168292.0 64 168292.0 65 168292.0 66 128381.0 67 88470.0 68 88470.0 69 88470.0 70 64578.5 71 40687.0 72 40687.0 73 40687.0 74 28655.5 75 16624.0 76 16624.0 77 16624.0 78 11353.5 79 6083.0 80 6083.0 81 6083.0 82 4075.5 83 2068.0 84 2068.0 85 2068.0 86 1305.0 87 542.0 88 542.0 89 542.0 90 357.0 91 172.0 92 172.0 93 172.0 94 111.5 95 51.0 96 51.0 97 51.0 98 44.0 99 37.0 100 37.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10189654146682921 2 0.05468236759462138 3 0.05818440193903 4 0.05366973115768395 5 0.05046304910738208 6 0.0702938459974068 7 0.06577917521606075 8 0.06253029998088648 9 0.06790993105211661 10 0.06818418675378717 11 0.061813015838055804 12 0.06280455568255704 13 0.0630366181993552 14 0.06586356158580554 15 0.06824747653109575 16 0.0636695159724411 17 0.06622220365722088 18 0.1461360958055333 19 0.07168622109819578 20 0.06514627744297485 21 0.06253029998088648 22 0.06828966971596813 23 0.06755128898070126 24 0.07725572150135165 25 0.07174951087550437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4740102.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.59637432989058 #Duplication Level Percentage of deduplicated Percentage of total 1 87.08458164870251 66.70363214325937 2 9.418344527882065 14.428220860510624 3 1.8885253526820116 4.3396258453656 4 0.6121846337261303 1.8756449345557455 5 0.2806669662369167 1.0749036003958818 6 0.1689433460854714 0.7764268666384213 7 0.09705779672353473 0.5203992730629207 8 0.078399129817989 0.48040712757410964 9 0.058221549632890286 0.4013603648772452 >10 0.281682840290383 3.8455351683644965 >50 0.01754863075946121 0.9345186053221612 >100 0.01137329746689326 1.6088396001896363 >500 0.0014433106782441342 0.7694016368944191 >1k 8.881896782611902E-4 1.3555610332885464 >5k 8.326778233622814E-5 0.44161342800985204 >10k+ 5.551185489081876E-5 0.44390951169101245 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 10575 0.22309646501277822 No Hit CCCATGTACTCTGCGTTGATACCAC 10305 0.21740038505500514 No Hit GTATCAACGCAGAGTACTTTTTTTT 9845 0.20769595253435474 No Hit GGTATCAACGCAGAGTACTTTTTTT 5621 0.11858394608386064 No Hit TATCAACGCAGAGTACTTTTTTTTT 5306 0.11193851946645875 No Hit GAGTACATGGGAAGCAGTGGTATCA 4802 0.10130583687861569 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.109659243619652E-5 0.0 4 0.0 0.0 0.0 2.109659243619652E-5 0.0 5 0.0 0.0 0.0 2.109659243619652E-5 0.0 6 0.0 0.0 0.0 2.109659243619652E-5 0.0 7 0.0 0.0 0.0 2.109659243619652E-5 0.0 8 0.0 0.0 0.0 2.109659243619652E-5 0.0 9 2.109659243619652E-5 2.109659243619652E-5 0.0 4.219318487239304E-5 0.0 10 2.109659243619652E-5 2.109659243619652E-5 0.0 4.219318487239304E-5 0.0 11 2.109659243619652E-5 2.109659243619652E-5 0.0 4.219318487239304E-5 0.0 12 2.109659243619652E-5 2.109659243619652E-5 0.0 6.328977730858956E-5 0.0 13 2.109659243619652E-5 2.109659243619652E-5 0.0 6.328977730858956E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 90 5.422353E-7 12.664823 19 AGGACCG 95 1.3656694E-5 10.997121 5 CTATGCG 80 3.7851E-4 10.684026 9 GTCTTAG 490 0.0 10.2752075 1 GGACCGT 85 6.616716E-4 10.055767 6 CGAACCG 125 2.559913E-6 9.883988 12 TAGGACC 335 0.0 9.639267 4 CCCCGTA 300 0.0 9.504035 15 CTGGTCG 90 0.0011177183 9.496912 9 GTCCTAA 400 0.0 9.262227 1 CGCCCTA 320 0.0 9.207327 16 AAATCGC 135 6.81436E-6 9.152421 17 TAGACAG 720 0.0 9.101784 5 CTAGGAC 515 0.0 9.036469 3 GCGTCGG 95 0.0018148861 9.005441 18 AGCGTAC 380 0.0 8.999735 1 TAACGTG 85 0.0074554337 8.938837 5 CCACGAC 85 0.0074554337 8.938837 3 GTATAGG 590 0.0 8.855671 1 GTCTTAC 455 0.0 8.768972 1 >>END_MODULE