FastQCFastQC Report
Wed 25 May 2016
SRR1294647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4740102
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT256910.5419925562783248No Hit
GGTATCAACGCAGAGTACTTTTTTT164260.34653262735696405No Hit
TATCAACGCAGAGTACTTTTTTTTT151820.32028846636633557No Hit
CCCATGTACTCTGCGTTGATACCAC115530.2437289324153784No Hit
GTACATGGGAAGCAGTGGTATCAAC105270.22208382857584075No Hit
ACGCAGAGTACTTTTTTTTTTTTTT92610.195375542551616No Hit
GAGTACATGGGAAGCAGTGGTATCA49400.10421716663481081No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49310.10402729730288506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC1201.3091994E-711.063642518
TAGACAG7350.010.2323575
TAGCACG951.6109238E-410.02102854
TATTCCG2002.3646862E-119.9959765
CAGGACT6700.09.6620664
ATCGTTC1201.66037E-59.5199788
CACGACA800.00444070589.5199774
GCGTTCT800.0045592219.48962911
AAATCGC1304.3407017E-69.48312217
AGACCGT1252.6893065E-59.1391786
TAGACTG6050.08.811885
TATACTG9200.08.795635
CCGTACA3150.08.730494517
CCAGGAC7650.08.6920373
TACGCTG1107.001277E-48.6545245
CCCCGTA2850.08.65117815
GCGACTA3750.08.59803116
TTAGACA5650.08.5932534
GTCTAGG4100.08.58120251
TGTCCCG3100.08.57127811
Produced by FastQC (version 0.11.5)